Echocardiographic evaluation of left atrial performance by using left atrial appendage flow velocity

Background:  Left atrial (LA) functional Study has a traditional role in evaluation of left ventricular diastolic function. The widely accepted echocardiographic parameters used in routine clinical practice to assess left ventricular diastolic function include pulsed-wave doppler mitral inflow analysis, tissue doppler imaging measurements, and LA dimension estimation. Left atrial appendage doppler studies using early diastolic emptying velocity, late diastolic emptying velocity and LAA filling velocity imaging to assess LA function were validated in patients with valvular atrial fibrillation (AF). Currently, there is no evidence regarding the Left atrial appendage doppler studies used in identifying the performance of left atrium and diastolic function in patients with Normal Sinus Rhythm. Objectives: This study aimed to identify the performance of left atrial function in patients with normal sinus rhythm using Left atrial appendage doppler studies. Methods: In a study population of 63 patients aged >18 years old who undergone routine echocardiographic study for Cardiac evaluation between Jan 2017 to Jan 2018, among which 40 (64%) were males and 23 (36%) were females. For those Patient’s, left atrial appendage flow velocity and diastolic doppler parameters are noted from G E echocardiography machine by the single observer. Results: The Mean Left Atrial Appendage (late-diastolic emptying velocity) in diastolic dysfunction patients (39.74) associated with impaired left atrial function, when compared with the mean Left Atrial Appendage (late-diastolic emptying velocity) in patients without diastolic dysfunction (54.76) respectively. Conclusion: In this study, we founded that Patient with diastolic dysfunction had an impaired left atrial function that was measured by left atrial appendage flow velocity

Extension of Raw Cow Milk Shelf Life by Microplasma Discharge

Cow's milk, the universal nutrient, is being stored and supplied, which seeks proper preservation. The prevalent milk preservation procedure of refrigeration, is effective only for two days, and after which, it starts to contaminate due to the growth of various milk-laden bacteria. This bacterial overload has to be inactivated properly to increase its shelf life, and is been achieved effectively using microplasma, a single-step, cost-effective and chemical-free process. Raw milk was treated for 5, 10, and 13 seconds in microplasma discharge. After 13 seconds of microplasma treatment, E. Coli, Pseudomonas, and S. Aureus bacteria got reduced at a respective rate of 89.93, 84.55, and 94.19% for in raw milk. The reactive species formed during microplasma discharge disrupts the structural integrity of bacterial cells and inactivates it, thereby enhancing the milk shelf life. Treated samples remained in good condition for 8 days. Thus, microplasma discharge increases the shelf life of milk by quickly inactivating the bacterial load

Isolation and screening of proteolytic bacteria from freshwater fish Cyprinus carpio

The present study was undertaken to isolate and characterize the protease producing potent bacteria from gastro intestinal tract of the freshwater fish Cyprinus carpio. The protease producer was characterized as Bacillus sp. morphologically and biochemically. The optimization studies for growth and proteases production of the isolate Bacillus sp. was carried out. The optimum temperature for the isolated Bacillus sp. was 350C but the optimum temperature for the protease production was 450C, the optimum pH was 7.0 in which the isolate produced 18 mMol/ml and showed a remarkable number of Colony Forming Unit. Hence, it was focused to study the intestinal Bacillus sp. in freshwater fish and its protease enzyme.&nbsp

A case study of bilayered spin-1/21/2 square lattice compound [VO(HCOO)2⋅_2\cdot(H2_2O)]

We present the synthesis and a detail investigation of structural and magnetic properties of polycrystalline [VO(HCOO)$_2\cdot$(H$_2$O)] by means of x-ray diffraction, magnetic susceptibility, high-field magnetization, heat capacity, and electron spin resonance measurements. It crystallizes in a orthorhombic structure with space group $Pcca$. It features distorted VO$_6$ octahedra connected via HCOO linker (formate anions) forming a two-dimensional square lattice network with a bilayered structure. Analysis of magnetic susceptibility, high field magnetization, and heat capacity data in terms of the frustrated square lattice model unambiguously establish quasi-two-dimensional nature of the compound with nearest neighbour interaction $J_1/k_{\rm B} \simeq 11.7$~K and next-nearest-neighbour interaction $J_2/k_{\rm B} \simeq 0.02$~K. It undergoes a N\'eel antiferromagnetic ordering at $T_{\rm N} \simeq 1.1$~K. The ratio $\theta_{\rm CW}/T_{\rm N} \simeq 10.9$ reflects excellent two-dimensionality of the spin-lattice in the compound. A strong in-plane anisotropy is inferred from the linear increase of $T_{\rm N}$ with magnetic field, consistent with the structural data.Comment: 9 pages, 7 figures, 1 tabl

Synthesis of triazole-linked morpholino oligonucleotides via Cu1 catalysed cycloaddition

Triazole-linked morpholino (TLMO) oligonucleic acids were synthesised using the CuI catalysed (3 + 2) azide–alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the TLMO modification gives higher Tm values than the corresponding TLDNA modification

Genome-Wide Analysis of the Complex Transcriptional Networks of Rice Developing Seeds

<div><h3>Background</h3><p>The development of rice (<em>Oryza sativa</em>) seed is closely associated with assimilates storage and plant yield, and is fine controlled by complex regulatory networks. Exhaustive transcriptome analysis of developing rice embryo and endosperm will help to characterize the genes possibly involved in the regulation of seed development and provide clues of yield and quality improvement.</p> <h3>Principal Findings</h3><p>Our analysis showed that genes involved in metabolism regulation, hormone response and cellular organization processes are predominantly expressed during rice development. Interestingly, 191 transcription factor (TF)-encoding genes are predominantly expressed in seed and 59 TFs are regulated during seed development, some of which are homologs of seed-specific TFs or regulators of <em>Arabidopsis</em> seed development. Gene co-expression network analysis showed these TFs associated with multiple cellular and metabolism pathways, indicating a complex regulation of rice seed development. Further, by employing a cold-resistant <em>cultivar</em> Hanfeng (HF), genome-wide analyses of seed transcriptome at normal and low temperature reveal that rice seed is sensitive to low temperature at early stage and many genes associated with seed development are down-regulated by low temperature, indicating that the delayed development of rice seed by low temperature is mainly caused by the inhibition of the development-related genes. The transcriptional response of seed and seedling to low temperature is different, and the differential expressions of genes in signaling and metabolism pathways may contribute to the chilling tolerance of HF during seed development.</p> <h3>Conclusions</h3><p>These results provide informative clues and will significantly improve the understanding of rice seed development regulation and the mechanism of cold response in rice seed.</p> </div

Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon

BACKGROUND: Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolerance, flowering time, and seed development. The aim of the current research was to identify and characterize NF-Y families in the emerging monocot model plant Brachypodium distachyon (Brachypodium) with the long term goal of assisting in the translation of known dicot NF-Y functions to the grasses. METHODOLOGY/PRINCIPAL FINDINGS: We identified, annotated, and further characterized 7 NF-YA, 17 NF-YB, and 12 NF-YC proteins in Brachypodium (BdNF-Y). By examining phylogenetic relationships, orthology predictions, and tissue-specific expression patterns for all 36 BdNF-Y, we proposed numerous examples of likely functional conservation between dicots and monocots. To test one of these orthology predictions, we demonstrated that a BdNF-YB with predicted orthology to Arabidopsis floral-promoting NF-Y proteins can rescue a late flowering Arabidopsis mutant. CONCLUSIONS/SIGNIFICANCE: The Brachypodium genome encodes a similar complement of NF-Y to other sequenced angiosperms. Information regarding NF-Y phylogenetic relationships, predicted orthologies, and expression patterns can facilitate their study in the grasses. The current data serves as an entry point for translating many NF-Y functions from dicots to the genetically tractable monocot model system Brachypodium. In turn, studies of NF-Y function in Brachypodium promise to be more readily translatable to the agriculturally important grasses