Performance Evaluation of Two 25 kW Residential Wood Pellet Boiler Heating Systems

A significant increase in the use of wood pellets for residential space heating has occurred over the past decade. The performance of two modern residential wood pellet boilers (designated PB and WPB) were evaluated including boiler thermal efficiency, thermal energy storage (TES) tank discharge efficiency, and system efficiency. A correlation applicable to both systems between the boiler thermal efficiency (ηth, in %) and the boiler output load (χ, in %) was found in the form of ηth = 52.69 ln χ – 137.7 with R2 = 0.79 (for 25 < χ < 75). This equation provides an easy, accurate estimation of the boiler thermal efficiency in field operations. The boiler thermal efficiency decreased with time and this decline was determined using a Mann-Kendall trend analysis with Sen’s slope. This decrease was primarily the result of fouling in the heat exchanger and thus, this analysis identifies the need for manual cleaning of the heat exchanger tubes to restore maximal system performance. The evaluation of the TES tank performance found that the TES tank discharge efficiency was correlated with a dimensionless function of tank inlet Reynolds number (Red) and temperature differences in the tank and inlet and outlet pipes. Overall system efficiency showed a seasonal average of 62.8%, 62.0%, and 75.8% for three heating seasons of the PB system. These results provide a comprehensive performance evaluation of these wood pellet boiler heating systems in the field over an extended period of operation

Nondestructive Evaluation of Weld seam Failures in Tubes and Other Structures for Thermal, Wind and Nuclear Power Applications

The paper gives inspection and maintenance practices being followed in an important Tube manufacturing plant (Bundy India Limited, Baroda) where the first author (R.M Joshi) worked. Advances in the eddy current technique of on line surface and subsurface inspection along with some unconventional modern methods and their scope of appli-cation have been highlighted. The inspection techniques contribute to improved customer confidence

Geospatial Technologies in the extraction of Groundwater Potential Zones

The utmost source which is contributing to the supply of water to the society is groundwater. Depletion of undergroundwater is enormously caused by eruptive growing rate of human population along with needs in the form of industrialand urbanized growth, indigent practices in irrigation methods and deforestation. As surface fresh water is very limit, waterdemand is drastically increasing for the needs. In this connection, there is immense predominant for the natural sourcecalled groundwater. It is mandatory for the communities not only to targeting the resource called groundwater but also toprovide remedial measures to replenishment the groundwater. Enormous investigations are in the process globally, to meetthe requirement to compensate resource for the needs in all aspects. Geospatial techniques are playing vital role in theextraction of groundwater resources by means of spatial and temporal data variations along with its integration analysis inthe form of separable layers to derive the solutions. In this connection, Nandyal mandal of Kurnool is selected to demarcatethe potential zones of groundwater by using this geospatial technology.The main motto of the research work is to identifythe effective potential groundwater zones by applying methodsand integration techniques of Geographic InformationSystem and remote sensing. This gives more information for the planning and management of the ground water.Byemploying geospatial technologies, the integrated composite output for potential zones is demarcated with help of keyparameters such as drainage, lineament, slope, geology, geomorphology, land use land cover and existing groundwaterlevels. All these thematic layers are extracted by using the satellite data and other available sources by using remote sensingand GIS. All these themes are demarcated using basic elements to identify the respective classes. Proper weightages areassignedtoeachclassofallthemesintheformofseparatecategorybasedonimportanceofweightagefromexcellenttopoor based on suitability to avail the groundwater sources. This process is applied for the considered themes and isreclassified based on results. It is then integrated with weighted overlay operation in ARCGIS environment. Appropriateweightage percentages which are equal to 100%, are given prior to overlay analysis of hydrology tools of ArcGIS. Variouszones like excellent, very good, good, moderate, poor are categorized for the integrated potential zones of groundwatersource. The use of suggested methodology is applied and demonstrated for a selected case study area in Nandyal Mandal ofKurnool District of Andhra Pradesh, India. Integrated output layout will be effectively useful in the demarcation of potentialresource zones. This demarcation area zone system is not only to identify the zones but also helpful in the replenishing theresources of the study area. The digital elevation model is also used to extract slope and drainage themes of the study area.The groundwater potential zones were obtained by overlaying all the thematic maps in terms of weighted overlay analysismethod using the spatial analysis tool in ArcGIS 10.1. This ground water potential information will be useful for effectiveidentificationofsuitablelocationsfor extractionofsuchgroundwater

Job Contracts

This chapter considers the distribution of job contracts — in terms of casual jobs, temporary jobs (that is, those of less than a year’s duration), and permanent jobs — across different subgroups of the population. Although the analysis of chapter 5 echoes that of chapter 3, which is cast in terms of regular salaried and wage employment and casual employment, the novelty of chapter 5 is two-fold. First, it explicitly addresses the question of job tenure: while much of the regular salaried and wage employment discussed in chapter 3 may have been permanent employment, some of it may not have been. Second, and more importantly, it addresses the issue of “desirable jobs” using a data set different from the NSS data used in the earlier chapter (that is, unit record data from the Indian Human Development Survey relating to the period 2011–12). The Survey provides details about the job tenure of persons by distinguishing between three types of jobs: casual (daily or piecework), contracts of less than one year duration (hereafter, simply, “contract jobs”), and permanent. The importance of the analysis contained in this chapter is that if one defines job insecurity as workers’ fear of involuntary job loss, job insecurity has negative consequences for employees’ attitudes towards their job, their health, and the quality of their relationship with their employers

A Dual-Color Fluorescence-Based Platform to Identify Selective Inhibitors of Akt Signaling

Background: Inhibition of Akt signaling is considered one of the most promising therapeutic strategies for many cancers. However, rational target-orientated approaches to cell based drug screens for anti-cancer agents have historically been compromised by the notorious absence of suitable control cells. Methodology/Principal Findings: In order to address this fundamental problem, we have developed BaFiso, a live-cell screening platform to identify specific inhibitors of this pathway. BaFiso relies on the co-culture of isogenic cell lines that have been engineered to sustain interleukin-3 independent survival of the parental Ba/F3 cells, and that are individually tagged with different fluorescent proteins. Whilst in the first of these two lines cell survival in the absence of IL-3 is dependent on the expression of activated Akt, the cells expressing constitutively-activated Stat5 signaling display IL-3 independent growth and survival in an Akt-independent manner. Small molecules can then be screened in these lines to identify inhibitors that rescue IL-3 dependence. Conclusions/Significance: BaFiso measures differential cell survival using multiparametric live cell imaging and permits selective inhibitors of Akt signaling to be identified. BaFiso is a platform technology suitable for the identification of smal

Akt-Induced Phosphorylation of N-CoR at Serine 1450 Contributes to Its Misfolded Conformational Dependent Loss (MCDL) in Acute Myeloid Leukemia of the M5 Subtype

10.1371/journal.pone.0070891PLoS ONE88-POLN

Albiglutide, a Long Lasting Glucagon-Like Peptide-1 Analog, Protects the Rat Heart against Ischemia/Reperfusion Injury: Evidence for Improving Cardiac Metabolic Efficiency

BACKGROUND: The cardioprotective effects of glucagon-like peptide-1 (GLP-1) and analogs have been previously reported. We tested the hypothesis that albiglutide, a novel long half-life analog of GLP-1, may protect the heart against I/R injury by increasing carbohydrate utilization and improving cardiac energetic efficiency. METHODS/PRINCIPAL FINDINGS: Sprague-Dawley rats were treated with albiglutide and subjected to 30 min myocardial ischemia followed by 24 h reperfusion. Left ventricle infarct size, hemodynamics, function and energetics were determined. In addition, cardiac glucose disposal, carbohydrate metabolism and metabolic gene expression were assessed. Albiglutide significantly reduced infarct size and concomitantly improved post-ischemic hemodynamics, cardiac function and energetic parameters. Albiglutide markedly increased both in vivo and ex vivo cardiac glucose uptake while reducing lactate efflux. Analysis of metabolic substrate utilization directly in the heart showed that albiglutide increased the relative carbohydrate versus fat oxidation which in part was due to an increase in both glucose and lactate oxidation. Metabolic gene expression analysis indicated upregulation of key glucose metabolism genes in the non-ischemic myocardium by albiglutide. CONCLUSION/SIGNIFICANCE: Albiglutide reduced myocardial infarct size and improved cardiac function and energetics following myocardial I/R injury. The observed benefits were associated with enhanced myocardial glucose uptake and a shift toward a more energetically favorable substrate metabolism by increasing both glucose and lactate oxidation. These findings suggest that albiglutide may have direct therapeutic potential for improving cardiac energetics and function

Corticotropin Releasing Factor-Induced CREB Activation in Striatal Neurons Occurs via a Novel Gβγ Signaling Pathway

The peptide corticotropin-releasing factor (CRF) was initially identified as a critical component of the stress response. CRF exerts its cellular effects by binding to one of two cognate G-protein coupled receptors (GPCRs), CRF receptor 1 (CRFR1) or 2 (CRFR2). While these GPCRs were originally characterized as being coupled to Gαs, leading to downstream activation of adenylyl cyclase (AC) and subsequent increases in cAMP, it has since become clear that CRFRs couple to and activate numerous other downstream signaling cascades. In addition, CRF signaling influences the activity of many diverse brain regions, affecting a variety of behaviors. One of these regions is the striatum, including the nucleus accumbens (NAc). CRF exerts profound effects on striatal-dependent behaviors such as drug addiction, pair-bonding, and natural reward. Recent data indicate that at least some of these behaviors regulated by CRF are mediated through CRF activation of the transcription factor CREB. Thus, we aimed to elucidate the signaling pathway by which CRF activates CREB in striatal neurons. Here we describe a novel neuronal signaling pathway whereby CRF leads to a rapid Gβγ- and MEK-dependent increase in CREB phosphorylation. These data are the first descriptions of CRF leading to activation of a Gβγ-dependent signaling pathway in neurons, as well as the first description of Gβγ activation leading to downstream CREB phosphorylation in any cellular system. Additionally, these data provide additional insight into the mechanisms by which CRF can regulate neuronal function

Innate Immune Response of Human Plasmacytoid Dendritic Cells to Poxvirus Infection Is Subverted by Vaccinia E3 via Its Z-DNA/RNA Binding Domain

Plasmacytoid dendritic cells (pDCs) play important roles in antiviral innate immunity by producing type I interferon (IFN). In this study, we assess the immune responses of primary human pDCs to two poxviruses, vaccinia and myxoma virus. Vaccinia, an orthopoxvirus, was used for immunization against smallpox, a contagious human disease with high mortality. Myxoma virus, a Leporipoxvirus, causes lethal disease in rabbits, but is non-pathogenic in humans. We report that myxoma virus infection of human pDCs induces IFN-α and TNF production, whereas vaccinia infection does not. Co-infection of pDCs with myxoma virus plus vaccinia blocks myxoma induction effects. We find that heat-inactivated vaccinia (Heat-VAC; by incubating the virus at 55°C for 1 h) gains the ability to induce IFN-α and TNF in primary human pDCs. Induction of IFN-α in pDCs by myxoma virus or Heat-VAC is blocked by chloroquine, which inhibits endosomal acidification required for TLR7/9 signaling, and by inhibitors of cellular kinases PI3K and Akt. Using purified pDCs from genetic knockout mice, we demonstrate that Heat-VAC-induced type I IFN production in pDCs requires the endosomal RNA sensor TLR7 and its adaptor MyD88, transcription factor IRF7 and the type I IFN feedback loop mediated by IFNAR1. These results indicate that (i) vaccinia virus, but not myxoma virus, expresses inhibitor(s) of the poxvirus sensing pathway(s) in pDCs; and (ii) Heat-VAC infection fails to produce inhibitor(s) but rather produces novel activator(s), likely viral RNA transcripts that are sensed by the TLR7/MyD88 pathway. Using vaccinia gene deletion mutants, we show that the Z-DNA/RNA binding domain at the N-terminus of the vaccinia immunomodulatory E3 protein is an antagonist of the innate immune response of human pDCs to poxvirus infection and TLR agonists. The myxoma virus ortholog of vaccinia E3 (M029) lacks the N-terminal Z-DNA/RNA binding domain, which might contribute to the immunostimulating properties of myxoma virus

PRAS40 and PRR5-Like Protein Are New mTOR Interactors that Regulate Apoptosis

TOR (Target of Rapamycin) is a highly conserved protein kinase and a central controller of cell growth. TOR is found in two functionally and structurally distinct multiprotein complexes termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). In the present study, we developed a two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) based proteomic strategy to identify new mammalian TOR (mTOR) binding proteins. We report the identification of Proline-rich Akt substrate (PRAS40) and the hypothetical protein Q6MZQ0/FLJ14213/CAE45978 as new mTOR binding proteins. PRAS40 binds mTORC1 via Raptor, and is an mTOR phosphorylation substrate. PRAS40 inhibits mTORC1 autophosphorylation and mTORC1 kinase activity toward eIF-4E binding protein (4E-BP) and PRAS40 itself. HeLa cells in which PRAS40 was knocked down were protected against induction of apoptosis by TNFα and cycloheximide. Rapamycin failed to mimic the pro-apoptotic effect of PRAS40, suggesting that PRAS40 mediates apoptosis independently of its inhibitory effect on mTORC1. Q6MZQ0 is structurally similar to proline rich protein 5 (PRR5) and was therefore named PRR5-Like (PRR5L). PRR5L binds specifically to mTORC2, via Rictor and/or SIN1. Unlike other mTORC2 members, PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knock down of tuberous sclerosis complex 1 (TSC1) and TSC2. Hyperactivation of mTOR by TSC1/2 knock down enhanced apoptosis whereas PRR5L knock down reduced apoptosis. PRR5L knock down reduced apoptosis also in mTORC2 deficient cells. The above suggests that mTORC2-dissociated PRR5L may promote apoptosis when mTOR is hyperactive. Thus, PRAS40 and PRR5L are novel mTOR-associated proteins that control the balance between cell growth and cell death