Evaluating Terra MODIS Satellite Sensor Data Products for Maize Yield Estimation in South Africa

The Free State Province of the Republic of South Africa contains some of the most important maize-producing areas in South Africa. For this reason this province has also been selected as a Joint Experiment for Crop Assessment and Monitoring (JECAM, 2012) site representative of South Africa.The Terra (EOS AM-1) research satellite carries the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor. Two data products which are used in this research, created by the National Aeronautics and Space Administration (NASA) from the MODIS sensor, are the Normalised Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI).Objective yield points (OYP) are Global Positioning Points (GPS) that fieldworkers visit to record certain yield related data, including a final objective yield. Three research studies utilizing Terra MODIS data were performed. Study one utilised objective yields from the 2001/2002, 2004/2005 and 2005/2006 growth seasons for three provinces in South Africa. In study two, the 2006/2007 growth season OYP were extracted for the Free State province only. NDVI was downloaded from United States Geological Survey (USGS) for study one and two and extracted into a GIS for comparison with the OYP. For the Free State pilot study, study three, OYP from the 2001 to 2010 growth seasons were used. MODIS NDVI and EVI data were obtained from the Wide Area Monitoring Information System WAMIS portal for the data set of 35 points.Statistical analysis was done on the data of studies two and three. The 2006/2007 dataset yielded a R2 of 0.47 for all 225 points and 0.51 for the medium growth cultivar data only (186 data points), while the 2001 to 2010 growth season yielded an R2 of 0.63 for the medium yield group. Therefore it is concluded that Terra MODIS NDVI and EVI data can be utilized for maize yield estimation on points

Hands-On TAROT: Intercontinental use of the TAROT for Education and Public Outreach

The TAROT telescope has for primary goal the search for the prompt optical counterpart of Cosmic Gamma-Ray Bursts. It is a completely autonomous 25cm telescope installed near Nice (France), able to point any location of the sky within 1-2 seconds. The control, scheduling, and data processing activities are completely automated, so the instrument is completely autonomous. In addition to its un-manned modes, we added recently the possibility to remotely control the telescope, as a request of the "Hands-On Universe" (HOU) program for exchange of time within automatic telescopes for the education and public outreach. To this purpose we developed a simple control interface. A webcam was installed to visualize the telescope. Access to the data is possible through a web interface. The images can be processed by the HOU software, a program specially suited for use within the classroom. We experienced these feature during the open days of the University of California Berkeley and the Astronomy Festival of Fleurance (France). We plan a regular use for an astronomy course of the Museum of Tokyo, as well as for French schools. Not only does Hands-On TAROT gives the general public an access to professional astronomy, but it is also a more general tool to demonstrate the use of a complex automated system, the techniques of data processing and automation. Last but not least, through the use of telescopes located in many countries over the globe, a form of powerful and genuine cooperation between teachers and children from various countries is promoted, with a clear educational goal.Comment: 4 pages, Based on a demonstration presented at the ADASS X Conference, Boston, MA, USA, October 2000, to appear in ASP Conf. Serie

Imaging the spotty surface of Betelgeuse in the H band

This paper reports on H-band interferometric observations of Betelgeuse made at the three-telescope interferometer IOTA. We image Betelgeuse and its asymmetries to understand the spatial variation of the photosphere, including its diameter, limb darkening, effective temperature, surrounding brightness, and bright (or dark) star spots. We used different theoretical simulations of the photosphere and dusty environment to model the visibility data. We made images with parametric modeling and two image reconstruction algorithms: MIRA and WISARD. We measure an average limb-darkened diameter of 44.28 +/- 0.15 mas with linear and quadratic models and a Rosseland diameter of 45.03 +/- 0.12 mas with a MARCS model. These measurements lead us to derive an updated effective temperature of 3600 +/- 66 K. We detect a fully-resolved environment to which the silicate dust shell is likely to contribute. By using two imaging reconstruction algorithms, we unveiled two bright spots on the surface of Betelgeuse. One spot has a diameter of about 11 mas and accounts for about 8.5% of the total flux. The second one is unresolved (diameter < 9 mas) with 4.5% of the total flux. Resolved images of Betelgeuse in the H band are asymmetric at the level of a few percent. The MOLsphere is not detected in this wavelength range. The amount of measured limb-darkening is in good agreement with model predictions. The two spots imaged at the surface of the star are potential signatures of convective cells.Comment: 10 pages, 10 figures, accepted for publication in A&A, references adde

Dynamical Masses of Young Stars in Multiple Systems

We present recent measurements of the orbital motion in the young binaries DF Tau and ZZ Tau, and the hierarchical triple Elias 12, that were obtained with the Fine Guidance Sensors on the HST and at the Keck Observatory using adaptive optics. Combining these observations with previous measurements from the literature, we compute preliminary orbital parameters for DF Tau and ZZ Tau. We find that the orbital elements cannot yet be determined precisely because the orbital coverage spans only about 90 degr in position angle. Nonetheless, the range of possible values for the period and semi-major axis already defines a useful estimate for the total mass in DF Tau and ZZ Tau, with values of 0.90{+0.85}{-0.35} M_sun and 0.81{+0.44}{-0.25} M_sun, respectively, at a fiducial distance of 140 pc.Comment: 26 pages, 9 figures, accepted for publication in A

Genome-wide identification of microRNA and siRNA responsive to endophytic beneficial diazotrophic bacteria in maize

Background: Small RNA (sRNA) has been described as a regulator of gene expression. In order to understand the role of maize sRNA (Zea mays - hybrid UENF 506-8) during association with endophytic nitrogen-fixing bacteria, we analyzed the sRNA regulated by its association with two diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. Results: Deep sequencing analysis was done with RNA extracted from plants inoculated with H. seropedicae, allowing the identification of miRNA and siRNA. A total of 25 conserved miRNA families and 15 novel miRNAs were identified. A dynamic regulation in response to inoculation was also observed. A hypothetical model involving copper-miRNA is proposed, emphasizing the fact that the up-regulation of miR397, miR398, miR408 and miR528, which is followed by inhibition of their targets, can facilitate association with diazotrophic bacteria. Similar expression patterns were observed in samples inoculated with A. brasilense. Moreover, novel miRNA and siRNA were classified in the Transposable Elements (TE) database, and an enrichment of siRNA aligned with TE was observed in the inoculated samples. In addition, an increase in 24-nt siRNA mapping to genes was observed, which was correlated with an increase in methylation of the coding regions and a subsequent reduction in transcription. Conclusion: Our results show that maize has RNA-based silencing mechanisms that can trigger specific responses when plants interact with beneficial endophytic diazotrophic bacteria. Our findings suggest important roles for sRNA regulation in maize, and probably in other plants, during association with diazotrophic bacteria, emphasizing the up-regulation of Cu-miRNA

Observations of T-Tauri Stars using HST-GHRS: I. Far Ultraviolet Emission Lines

We have analyzed GHRS data of eight CTTS and one WTTS. The GHRS data consists of spectral ranges 40 A wide centered on 1345, 1400, 1497, 1550, and 1900 A. These UV spectra show strong SiIV, and CIV emission, and large quantities of sharp (~40 km/s) H2 lines. All the H2 lines belong to the Lyman band and all the observed lines are single peaked and optically thin. The averages of all the H2 lines centroids for each star are negative which may indicate that they come from an outflow. We interpret the emission in H2 as being due to fluorescence, mostly by Ly_alpha, and identify seven excitation routes within 4 A of that line. We obtain column densities (10^12 to 10^15 cm^-2) and optical depths (~1 or less) for each exciting transition. We conclude that the populations are far from being in thermal equilibrium. We do not observe any lines excited from the far blue wing of Ly_alpha, which implies that the molecular features are excited by an absorbed profile. SiIV and CIV (corrected for H2 emission) have widths of ~200 km/s, and an array of centroids (blueshifted lines, centered, redshifted). These characteristics are difficult to understand in the context of current models of the accretion shock. For DR Tau we observe transient strong blueshifted emission, perhaps the a result of reconnection events in the magnetosphere. We also see evidence of multiple emission regions for the hot lines. While CIV is optically thin in most stars in our sample, SiIV is not. However, CIV is a good predictor of SiIV and H2 emission. We conclude that most of the flux in the hot lines may be due to accretion processes, but the line profiles can have multiple and variable components.Comment: 67 pages, 19 figures, Accepted in Ap

A Test of Pre-Main Sequence Evolutionary Models Across the Stellar/Substellar Boundary Based on Spectra of the Young Quadruple GG Tau

We present spatially separated optical spectra of the components of the young hierarchical quadruple GG Tau. Spectra of GG Tau Aa and Ab (separation 0".25 ~ 35 AU) were obtained with the Faint Object Spectrograph aboard the Hubble Space Telescope. Spectra of GG Tau Ba and Bb (separation 1".48 ~ 207 AU) were obtained with both the HIRES and the LRIS spectrographs on the W. M. Keck telescopes. The components of this mini-cluster, which span a wide range in spectral type (K7 - M7), are used to test both evolutionary models and the temperature scale for very young, low mass stars under the assumption of coeval formation. Of the evolutionary models tested, those of Baraffe et al. (1998, A&A, 337, 403) yield the most consistent ages when combined with a temperature scale intermediate between that of dwarfs and giants. The version of the Baraffe et al. models computed with a mixing length nearly twice the pressure scale height is of particular interest as it predicts masses for GG Tau Aa and Ab that are in agreement with their dynamical mass estimate. Using this evolutionary model and a coeval (at 1.5 Myrs) temperature scale, we find that the coldest component of the GG Tau system, GG Tau Bb, is substellar with a mass of 0.044 +/- 0.006 Msun. This brown dwarf companion is especially intriguing as it shows signatures of accretion, although this accretion is not likely to alter its mass significantly. GG Tau Bb is currently the lowest mass, spectroscopically confirmed companion to a T Tauri star, and is one of the coldest, lowest mass T Tauri objects in the Taurus-Auriga star forming region.Comment: 25 pages, 6 figures, accepted for publication in The Astrophysical Journa

Differential sRNA Regulation in Leaves and Roots of Sugarcane under Water Depletion

Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638 - sensitive cultivar- and; SP83-2847 and SP83-5073 - tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion