Objectively measuring the effects of sleep on reading comprehension and sustained selective attention

The overall performance of a university is measured by retention rates of students. Because individuals who achieve lower grade point averages are at a higher risk of failing or dropping out of college, the academic performance of undergraduates should be the target of concern to maintain good retention rates. Academic performance, which is associated with attention and reading comprehension abilities, is affected by the sleep behavior of students. In regards to college students and sleep, research has indicated that college students demonstrate habitually poor sleep habits. Poor sleep habits have been linked to impaired attention and concentration abilities, but the measures used to quantify these associations have relied on self-report. Additionally, the effects of sleep and cognitive functioning has focused on clinical populations that meet the requirements of either a sleep disorder or Attention Deficit Hyperactivity Disorder, with limited research examining the effects of sleep behavior on a non-clinical population. The purpose of this study was to test the objective measures of sleep behavior and cognitive functioning with healthy young adults enrolled in an undergraduate university. Specifically, this study focused on the role that the different stages of sleep play in cognitive functioning in addition to the roles of one\u27s sleep quality and sleep quantity. Collected data were analyzed using one-way analysis of variance as well as multiple regression analyses. Results demonstrated that students\u27 sleep architecture (i.e., sleep quantity, time spent in different sleep stages), daytime sleepiness, or sleep quality did not significantly impact sustained attention or reading comprehension; however, computation of effect sizes revealed a strong effect for sleep quantity and reading comprehension

Whose Rights Should Prevail? Toward a Child-Centric Approach to Revocation of Birthparent Consent in Domestic Infant Abortion

Though overhaul of adoption laws across the United States has been nearly universal, there is still no uniformity among the states in approach to voluntary relinquishment of parental rights: the very issue at the heart of the controversial cases that sparked reform. This Note attempts to track the development of domestic adoption laws as they affect birthparent consent in infant adoptions, the competing policies driving these developments, and the way states have attempted to reconcile that friction. Part I of this Note provides an underpinning of adoption terminology by outlining the actors involved, the basic elements required for infant adoption in the United States, and how birthparent consent fits in the mix. Part II gives a brief historical background of adoption statutes in the United States, including more recent attempts at uniformity in adoption laws across the fifty states. In Part III, this Note addresses competing policy interests and the friction that arises among the dueling rights of the various actors in the adoption process. Part III also attempts to grasp a firm definition of the ever-elusive “best interests of the child” standard, taking the position that the child’s interest in stability and permanency must be the ultimate goal of the law in the adoption context. Part IV categorizes current statutes across the fifty states into three distinct groups, drawing heavily on data compiled in APPENDIX A concerning certain identified criteria in each state’s adoption laws. Finally, Part V proposes a model statute that falls squarely into the most conservative of these categories, balancing the interests and rights of the various actors with the best interests of the child being held paramount. The proposal argues, first, for only a very brief delay of birthparent consent after the child’s birth and, second, for no subsequent revocation period. Though the primary purpose of this limitation is stability for the child, this Note will show that this design best serves each of the other actors as well

Studies of sidewall boundary layer in the Langley 0.3 meter transonic cryogenic tunnel with and without suction

Boundary layer measurements on the sidewalls of the Langley 0.3 Meter Transonic Cryogenic Tunnel were made to determine the effectiveness of the passive boundary layer bleed system over a Reynolds number range from 20 to 200 x 10 to the sixth power per meter at Mach numbers from 0.30 to 0.76. The tunnel sidewall boundary layer displacement thickness was about 2 percent of the width of the test section without the boundary layer bleed. Measured velocity profiles correlated well with the defect law of Hama. With the boundary layer bleed equivalent to about 2 percent of the test section mass flow, the boundary layer displacement thickness reduced to about 1 percent of the test section width, which is generally considered acceptable for testing airfoils. It was also noticed that effectiveness of the bleed was nearly independent of the Mach number and Reynolds number over the range of conditions tested. A comparison of the measured suction effectiveness of the bleed with the finite difference and integral methods of boundary layer calculation showed good agreement

Using KCWI to Explore the Chemical Inhomogeneities and Evolution of J1044+0353

J1044+0353 is considered a local analog of the young galaxies that ionized the intergalactic medium at high-redshift due to its low mass, low metallicity, high specific star formation rate, and strong high-ionization emission lines. We use integral field spectroscopy to trace the propagation of the starburst across this small galaxy using Balmer emission- and absorption-line equivalent widths and find a post-starburst population (~ 15 - 20 Myr) roughly one kpc east of the much younger, compact starburst (~ 3 - 4 Myr). Using the direct electron temperature method to map the O/H abundance ratio, we find similar metallicity (1 to 3 sigma) between the starburst and post-starburst regions but with a significant dispersion of about 0.3 dex within the latter. We also map the Doppler shift and width of the strong emission lines. Over scales several times the size of the galaxy, we discover a velocity gradient parallel to the galaxy's minor axis. The steepest gradients (~ 30 $\mathrm{km \ s^{-1} \ kpc^{-1}}$) appear to emanate from the oldest stellar association. We identify the velocity gradient as an outflow viewed edge-on based on the increased line width and skew in a biconical region. We discuss how this outflow and the gas inflow necessary to trigger the starburst affect the chemical evolution of J1044+0353. We conclude that the stellar associations driving the galactic outflow are spatially offset from the youngest association, and a chemical evolution model with a metal-enriched wind requires a more realistic inflow rate than a homogeneous chemical evolution model.Comment: 23 pages, 10 figure

A Miniaturized Enzymatic Biosensor for Detection of Sensory-Evoked D-serine Release in the Brain

D-serine has been implicated as a brain messenger with central roles in neural signaling and plasticity. Disrupted levels of D-serine in the brain have been associated with neurological disorders, including schizophrenia, depression and Alzheimer's disease. Electrochemical biosensors are attractive tools for measuring real-time in vivo D-serine concentration changes. Current biosensors suffer from relatively large sizes (≥25 μm) making localized cellular measurements challenging, especially for single cell studies. In this work, a robust methodology for the fabrication of a reproducible miniaturized 10 μm D-serine detecting amperometric biosensor was developed. The miniature biosensor incorporated yeast D-amino acid oxidase immobilized on a poly-meta-phenylenediamine modified 10 μm Pt disk microelectrode. The biosensor offered a limit of detection of 0.361 μM (RSD < 10%) with high sensitivity (283 μA cm-2 mM-1, R2 = 0.983). The biosensor was stable for over four hours of continuous use, demonstrated a storage stability of four days and high analyte selectivity. Biosensor selectivity was validated with LC-MS and interferences with yeast D-amino acid oxidase were evaluated using drugs believed to stimulate D-serine release. Ex vivo D-serine measurements were made from Xenopus laevis tadpole brains, demonstrating the utility of the biosensors for measurements on living tissue. We observed that D-serine levels in the brain fluctuate with sensory experience. The biosensors were also used in vivo successfully. Taken together, this study addresses factors for successful and reproducible miniature biosensor fabrication for measuring D-serine in biological samples, for pharmacological evaluation, and for designing point of care devices

The role of the proteins Kar9 and Myo2 in orienting the mitotic spindle of budding yeast

AbstractBackground: Two genetic ‘pathways' contribute to the fidelity of nuclear segregation during the process of budding in the yeast Saccharomyces cerevisiae. An early pathway, involving Kar9p and other proteins, orients the mitotic spindle along the mother–bud axis. Upon the onset of anaphase, cytoplasmic dynein provides the motive force for nuclear movement into the bud. Loss of either pathway results in nuclear-migration defects; loss of both is lethal. Here, to visualize the functional steps leading to correct spindle orientation along the mother–bud axis, we imaged live yeast cells expressing Kar9p and dynein as green fluorescent protein fusions.Results: Transport of Kar9p into the bud was found to require the myosin Myo2p. Kar9p interacted with microtubules through the microtubule-binding protein Bim1p and facilitated microtubule penetration into the bud. Once microtubules entered the bud, Kar9p provided a platform for microtubule capture at the bud cortex. Kar9p was also observed at sites of microtubule shortening in the bud, suggesting that Kar9p couples microtubule shortening to nuclear migration.Conclusions: Thus, Kar9p provides a key link between the actin cytoskeleton and microtubules early in the cell cycle. A cooperative mechanism between Kar9p and Myo2p facilitates the pre-anaphase orientation of the spindle. Later, Kar9p couples microtubule disassembly with nuclear migration

Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells

A previously engineered Methanocaldococcus jannaschii –tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting -tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent protein, GFP, and in a foldon protein in mammalian cells. The methodology reported here will allow rapid transformation of the much larger collection of existing tyrosyl-tRNA synthetases that were already evolved for the incorporation of an array of over 50 unnatural amino acids into proteins in Escherichia coli into proteins in mammalian cells. Thus we will be able to introduce a large array of possibilities for protein modifications in mammalian cells

Brain microvasculature defects and Glut1 deficiency syndrome averted by early repletion of the glucose transporter-1 protein

Haploinsufficiency of the SLC2A1 gene and paucity of its translated product, the glucose transporter-1 (Glut1) protein, disrupt brain function and cause the neurodevelopmental disorder, Glut1 deficiency syndrome (Glut1 DS). There is little to suggest how reduced Glut1 causes cognitive dysfunction and no optimal treatment for Glut1 DS. We used model mice to demonstrate that low Glut1 protein arrests cerebral angiogenesis, resulting in a profound diminution of the brain microvasculature without compromising the blood–brain barrier. Studies to define the temporal requirements for Glut1 reveal that pre-symptomatic, AAV9-mediated repletion of the protein averts brain microvasculature defects and prevents disease, whereas augmenting the protein late, during adulthood, is devoid of benefit. Still, treatment following symptom onset can be effective; Glut1 repletion in early-symptomatic mutants that have experienced sustained periods of low brain glucose nevertheless restores the cerebral microvasculature and ameliorates disease. Timely Glut1 repletion may thus constitute an effective treatment for Glut1 DS

Levels, sources and chemical fate of persistent organic pollutants in the atmosphere and snow along the western Antarctic Peninsula

The Antarctic continent is among the most pristine regions; yet various organic contaminants have been measured there routinely. Air and snow samples were collected during the austral spring (October November, 2010) along the western Antarctic Peninsula and analyzed for organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) to assess the relative importance of long-range transport versus local primary or secondary emissions. Highest concentrations of PCBs, PBDEs and DDTs were observed in the glacier\u27s snow sample, highlighting the importance of melting glaciers as a possible secondary source of legacy pollutants to the Antarctic. In the atmosphere, contaminants were mainly found in the vapor phase (\u3e65%). Hexachlorobenzene (33.6 pg/m(3)), PCBs (11.6 pg/m(3)), heptachlor (5.64 pg/m(3)), PBDEs (4.22 pg/m(3)) and cis-chlordane (2.43 pg/m(3)) were the most abundant contaminants. In contrast to other compounds, PBDEs seem to have originated from local sources, possibly the research station itself. Gas-particle partitioning for analytes were better predicted using the adsorption partitioning model than an octanol-based absorption approach. Diffusive flux calculations indicated that net deposition is the dominant pathway for PBDEs and chlordanes, whereas re-volatilization from snow (during melting or metamorphosis) was observed for PCBs and some OCPs. (C) 2016 Elsevier Ltd. All rights reserved

First-Order Contaminant Removal in the Hyporheic Zone of Streams: Physical Insights from a Simple Analytical Model

A simple analytical model is presented for the removal of stream-borne contaminants by hyporheic exchange across duned or rippled streambeds. The model assumes a steady-state balance between contaminant supply from the stream and first-order reaction in the sediment. Hyporheic exchange occurs by bed form pumping, in which water and contaminants flow into bed forms in high-pressure regions (downwelling zones) and out of bed forms in low-pressure regions (upwelling zones). Model-predicted contaminant concentrations are higher in downwelling zones than upwelling zones, reflecting the strong coupling that exists between transport and reaction in these systems. When flow-averaged, the concentration difference across upwelling and downwelling zones drives a net contaminant flux into the sediment bed proportional to the average downwelling velocity. The downwelling velocity is functionally equivalent to a mass transfer coefficient, and can be estimated from stream state variables including stream velocity, bed form geometry, and the hydraulic conductivity and porosity of the sediment. Increasing the mass transfer coefficient increases the fraction of streamwater cycling through the hyporheic zone (per unit length of stream) but also decreases the time contaminants undergo first-order reaction in the sediment. As a consequence, small changes in stream state variables can significantly alter the performance of hyporheic zone treatment systems