Offrir à l'oeil : une phénoménologie du récit Au Château d'Argol de Julien Gracq

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Electron photodetachment dissociation of DNA anions with covalently or noncovalently bound chromophores

Double stranded DNA multiply charged anions coupled to chromophores were subjected to UV-Vis photoactivation. in a quadrupole ion trap mass spectrometer. The chromophores included noncovalently bound minor groove binders (activated in the near UV), noncovalently bound intercalators (activated with visible light), and covalently linked fluorophores and quenchers (activated at their maximum absorption wavelength). We found that the activation of only chromophores having long fluorescence lifetimes did result in efficient electron photodetachment from the DNA complexes. In the case of ethidium-dsDNA complex excited at 500 nm, photodetachment is a multiphoton process. The MS3 fragmentation of radicals produced by photodetachment at lambda = 260 nm (DNA excitation) and by photodetachment at lambda > 300 nm (chromophore excitation) were compared. The radicals keep no memory of the way they were produced. A weakly bound noncovalent ligand (m-amsacrine) allowed probing experimentally that a fraction of the electronic internal energy was converted into vibrational internal energy. This fragmentation channel was used to demonstrate that excitation of the quencher DABSYL resulted in internal conversion, unlike the fluorophore 6-FAM. Altogether, photodetachment of the DNA complexes upon chromophore excitation can be interpreted by the following mechanism: (1) ligands with sufficiently long excited-state lifetime undergo resonant two-photon excitation to reach the level of the DNA excited states, then (2) the excited-state must be coupled to the DNA excited states for photodetachment to occur. Our experiments also pave the way towards photodissociation probes of biomolecule conformation in the gas-phase by Forster resonance energy transfer (FRET)

Sheared sheet intrusions as mechanism for lateral flank displacement on basaltic volcanoes: Applications to Réunion Island volcanoes

International audienceField work carried out on the Piton des Neiges volcano (Réunion Island) suggests that the injection of magma along detachments could trigger flank failure by conjugate opening and shear displacement. We use 3-D numerical models to compare the ability of purely opened sheet intrusions, sheared sheet intrusions, and normal faults to induce flank displacement on basaltic volcanoes. We assume that shear stress change on fractures results from stress anisotropy of the host rock under gravity. Exploring a large range of stress anisotropies, fracture dips, and fracture depth over length ratios, we determine that the amount of shear displacement is independent of the proximity to the ground surface. Sheared sheet intrusions are the most efficient slip medium on volcanoes. Consequently, the largest flank displacement is induced by the longest, deepest sheared intrusion dipping closest to 45° in a host rock with the highest stress anisotropy. Using our model in a forward way, we provide shear and normal displacements for buried fractures. Applying the model to a pile of sills at the Piton des Neiges volcano, we determine that the mean shear displacement caused by each intrusion was 3.7 m, leading to a total of a 180–260 m of lateral displacement for the 50 m high pile of sills. Using our model in an inverse way, we formulate a decision tree to determine some fracture characteristics and the host rock stress anisotropy from ratios of maximum surface displacements. This procedure provides a priori models, which can be used to bound the parameter space before it is explored through a formal inversion. Applying the decision tree to the 1.4 m coeruptive flank displacement recorded at Piton de la Fournaise in 2007, we find that it probably originated from a shallow eastward dipping subhorizontal normal fault

Draft Genome Sequences of Four Aeromonas salmonicida subsp. achromogenes Strains, 23051, 23053, 23055, and 23056, Isolated from Senegalese Sole ( Solea senegalensis )

International audienceThe bacterial species Aeromonas salmonicida officially has five subspecies. A large majority of the currently available sequences come from Aeromonas salmonicida subsp. salmonicida, which causes furunculosis in salmonids. We present the genomic sequences of four Aeromonas salmonicida subsp. achromogenes strains. This will help increase the robustness of genomic analyses for this subspecies

The Citius End: World Records Progression Announces the Completion of a Brief Ultra-Physiological Quest

World records (WR) in sports illustrate the ultimate expression of human integrated muscle biology, through speed or strength performances. Analysis and prediction of man's physiological boundaries in sports and impact of external (historical or environmental) conditions on WR occurrence are subject to scientific controversy. Based on the analysis of 3263 WR established for all quantifiable official contests since the first Olympic Games, we show here that WR progression rate follows a piecewise exponential decaying pattern with very high accuracy (mean adjusted r2 values = 0.91±0.08 (s.d.)). Starting at 75% of their estimated asymptotic values in 1896, WR have now reached 99%, and, present conditions prevailing, half of all WR will not be improved by more than 0,05% in 2027. Our model, which may be used to compare future athletic performances or assess the impact of international antidoping policies, forecasts that human species' physiological frontiers will be reached in one generation. This will have an impact on the future conditions of athlete training and on the organization of competitions. It may also alter the Olympic motto and spirit

Psychological Balance in High Level Athletes: Gender-Based Differences and Sport-Specific Patterns

OBJECTIVES: Few epidemiological studies have focused on the psychological health of high level athletes. This study aimed to identify the principal psychological problems encountered within French high level athletes, and the variations in their prevalence based on sex and the sport practiced. METHODS: Multivariate analyses were conducted on nationwide data obtained from the athletes' yearly psychological evaluations. RESULTS: A representative sample of 13% of the French athlete population was obtained. 17% of athletes have at least one ongoing or recent disorder, generalized anxiety disorder (GAD) being the most prevalent (6%), followed by non-specific eating disorders (4.2%). Overall, 20.2% of women had at least one psychopathology, against 15.1% in men. This female predominance applied to anxiety and eating disorders, depression, sleep problems and self-harming behaviors. The highest rates of GAD appeared in aesthetic sports (16.7% vs. 6.8% in other sports for men and 38.9% vs. 10.3% for women); the lowest prevalence was found in high risk sports athletes (3.0% vs. 3.5%). Eating disorders are most common among women in racing sports (14% vs. 9%), but for men were found mostly in combat sports (7% vs. 4.8%). DISCUSSION: This study highlights important differences in psychopathology between male and female athletes, demonstrating that the many sex-based differences reported in the general population apply to elite athletes. While the prevalence of psychological problems is no higher than in the general population, the variations in psychopathology in different sports suggest that specific constraints could influence the development of some disorders

Success in Developing Regions: World Records Evolution through a Geopolitical Prism

A previous analysis of World Records (WR) has revealed the potential limits of human physiology through athletes' personal commitment. The impact of political factors on sports has only been studied through Olympic medals and results. Here we studied 2876 WR from 63 nations in four summer disciplines. We propose three new indicators and show the impact of historical, geographical and economical factors on the regional WR evolution. The south-eastward path of weighted annual barycenter (i.e. the average of country coordinates weighting by the WR number) shows the emergence of East Africa and China in WR archives. Home WR ratio decreased from 79.9% before the second World War to 23.3% in 2008, underlining sports globalization. Annual Cumulative Proportions (ACP, i.e. the cumulative sum of the WR annual rate) highlight the regional rates of progression. For all regions, the mean slope of ACP during the Olympic era is 0.0101, with a maximum between 1950 and 1989 (0.0156). For European countries, this indicator reflects major historical events (slowdown for western countries after 1945, slowdown for eastern countries after 1990). Mean North-American ACP slope is 0.0029 over the century with an acceleration between 1950 and 1989 at 0.0046. Russia takes off in 1935 and slows down in 1988 (0.0038). For Eastern Europe, maximal progression is seen between 1970 and 1989 (0.0045). China starts in 1979 with a maximum between 1990 and 2008 (0.0021), while other regions have largely declined (mean ACP slope for all other countries  = 0.0011). A similar trend is observed for the evolution of the 10 best performers. The national analysis of WR reveals a precise and quantifiable link between the sport performances of a country, its historical or geopolitical context, and its steps of development

Conception d'un système modulaire de collecte de données embarqué sur le drone marin PAMELI

International audienceThe PAMELI project (Multidimensional Autonomous Platform for Interdisciplinary Littoral Exploration) focuses on the repeated observation of environmental parameters such as physico-chemical parameters of the water column (Ph, salinity, conductivity), water depth and precise altimetry using a marine drone. This project first developed in Pertuis Charentais, since July 2018. One of the challenges of this project is to design an information system for data collection that is robust, reliable and flexible, ie to guarantee the good conditions for archiving and replaying data according to FAIR principles (Findable, Accessible, Interoperable and Reusable).This poster details the solutions put in place in an embedded information system to ensure this level of data quality requirements, and to meet the constraints of this embedded system (low energy resources, no Internet access, sensors removable, scalable and heterogeneous in their mode of communication). In particular, it shows how the operators of the drone can visualize the collected data in real time to intervene quickly in case of failure, or record annotations during the mission of the drone that will adjust the data qualification methods.Le projet PAMELI (Plateforme Autonome Multicapteurs pour l'Exploration Littorale Interdisciplinaire) porte sur l'observation répétée des paramètres environnementaux tels que les paramètres physico-chimiques de la colonne d'eau (Ph, salinité, conductivité), la profondeur d'eau et l'altimétrie précise à l'aide d'un drone marin. Ce projet se développe dans un premier temps dans les Pertuis Charentais, depuis juillet 2018. Un des enjeux de ce projet est de concevoir un système d'information pour la collecte des données qui soit robuste, fiable et flexible, i.e. permettant de garantir les bonnes conditions d'archivage et de rediffusion des données suivant les principes du FAIR (Findable, Accessible, Interoperable and Reusable). Ce poster détaille donc les solutions mises en place dans un système d’information embarqué pour assurer ce niveau d’exigences concernant la qualité des données, et de répondre aux contraintes de ce système embarqué (faibles ressources énergétiques, pas d’accès Internet, capteurs amovibles, évolutifs et hétérogènes dans leur mode de communication). En particulier, il montre comment les opérateurs du drone peuvent visualiser les données collectées en temps réel pour intervenir rapidement en cas de panne, ou enregistrer des annotations durant la mission du drone qui permettront d’ajuster les méthodes de qualification des données

Athlete Atypicity on the Edge of Human Achievement: Performances Stagnate after the Last Peak, in 1988

The growth law for the development of top athletes performances remains unknown in quantifiable sport events. Here we present a growth model for 41351 best performers from 70 track and field (T&F) and swimming events and detail their characteristics over the modern Olympic era. We show that 64% of T&F events no longer improved since 1993, while 47% of swimming events stagnated after 1990, prior to a second progression step starting in 2000. Since then, 100% of swimming events continued to progress

From Oxford to Hawaii Ecophysiological Barriers Limit Human Progression in Ten Sport Monuments

In order to understand the determinants and trends of human performance evolution, we analyzed ten outdoor events among the oldest and most popular in sports history. Best performances of the Oxford-Cambridge boat race (since 1836), the channel crossing in swimming (1875), the hour cycling record (1893), the Elfstedentocht speed skating race (1909), the cross country ski Vasaloppet (1922), the speed ski record (1930), the Streif down-hill in Kitzbühel (1947), the eastward and westward sailing transatlantic records (1960) and the triathlon Hawaii ironman (1978) all follow a similar evolutive pattern, best described through a piecewise exponential decaying model (r2 = 0.95±0.07). The oldest events present highest progression curvature during their early phase. Performance asymptotic limits predicted from the model may be achieved in fourty years (2049±32 y). Prolonged progression may be anticipated in disciplines which further rely on technology such as sailing and cycling. Human progression in outdoor sports tends to asymptotic limits depending on physiological and environmental parameters and may temporarily benefit from further technological progresses