10 research outputs found
Endophytic actinomycetes associated with Cinnamomum cassia Presl in Hoa Binh province, Vietnam: Distribution, antimicrobial activity and, genetic features
Endophytic microbes associated with medicinal plants are considered to be potential producers of various bioactive secondary metabolites. The present study investigated the distribution, antimicrobial activity and genetic features of endophytic actinomycetes isolated from the medicinal plant Cinnamomum cassia Press collected in Hoa Binh prov ince of northern Vietnam. Based on phenotypic characteristics, 111 actinomycetes were isolated from roots, stems and leaves of the host plants by using nine selective media. The isolated actinomycetes were mainly recovered from stems (n = 67; 60.4%), followed by roots (n = 29; 26.1%) and leaves (n = 15; 13.5%). The isolates were accordingly assigned into 5 color categories of aerial mycelium, of which gray is the most dominant (n = 42; 37.8%), followed by white (n = 33; 29.7%), yellow (n = 25; 22,5%), red (n = 8; 7.2%) and green (n = 3; 2.7%). Of the total endophytic actinomycetes tested, 38 strains (occupying 34.2%) showed antimicrobial activity against at least one of nine tested microbes and, among them, 26 actinomycetes (68.4%) revealed anthracycline-like antibiotics production. Analysis of 16S rRNA gene sequences deposited on GenBank (NCBI) of the antibioticproducing actinomycetes identified 3 distinct genera, including Streptomyces, Microbacterium, and Nocardia, among which Streptomyces genus was the most dominant and represented 25 different species. Further genetic investigation of the antibiotic-producing actinomycetes found that 28 (73.7%) and 11 (28.9%) strains possessed genes encoding polyketide synthase (pks) and non ribosomal peptide synthetase (nrps), respectively. The findings in the present study highlighted endophytic actinomycetes from C. cassia Presi which possessed broad-spectrum bioactivities with the potential for applications in the agricultural and pharmaceutical sectors.Y
Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities
Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragonâs blood. Excessive utilization of the plant for extraction of dragonâs blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33âÎŒg·mLâ1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria
Exploration de la biodiversité végétale et microbienne pour la qualité et la sureté des aliments
International audienceLa qualité et sureté des aliments jouent un rÎle important au Vietnam et dans la région de l'Asie du Sud Est. Dans son programme intégral, l'ASEAN met la sureté des aliments parmi des 12 priorités. Plusieurs travaux ont été focalises sur l'exploration de la biodiversité et des innovations technologiques en Asie du Sud Est afin d'améliorer la qualité et de gérer la sureté des aliments. C'est pour ces raisons, nous avons crée un réseau régional avec les soutiens des partenaires francophones et non-francophones en se basant sur les besoins de la région, l'expertise des partenaires et les coopérations étroites. Ce réseau est composé des 10 partenaires dynamiques et actifs provenant de la France, la Thaïlande, la Chine, et le Vietnam qui ont fait partie du Pre-projet de Coopération Scientifique Inter-universitaire PCSI http://pcsi2013.hust.edu.vn) de l'Agence Universitaire de la Francophonie (AUF) et le Laboratoire International Associe UB/AgroSup Dijon/HUST "Tropical Bioresources & Biotechnology"(www.umr-pam.fr/relation-internationale/tropical-bioresources-biotechnology.html). Notre présentation partagera nos expériences pour monter ce réseau a travers les régions et avec les partenaires francophones, souligner nos activités de recherche multidisciplinaires ainsi que notre point de vue vis-a-vis les interactions Nord-Sud et Sud-Sud. Nous allons aborder nos résultats issus de différents projets associes a ce réseau donc tels que la biodiversité des actinomycÚtes associes aux plantes médicinales, les bactéries lactiques dans les produits traditionnels fermentes du Vietnam et la valorisation de l'agriculture (riz, manioc) et leurs sous-produits par voies biologiques. (Résumé d'auteur