88 research outputs found
The clinical application of a new synthetic bone grafting material in oral and maxillofacial surgery
A novel bone formation material based on hydroxyapatite-xerogel is presented.
With the use of the innovative sol-gel technology this material is produced in the
low-temperature range by the addition of silicon dioxide; in its structure it mimics
to a great extent the natural bone matrix. This results in high osteoconductivity
and an osteoprotective effect as well as in complete biodegradation corresponding
to bone formation in the course of natural bone remodelling. Two case reports
are presented
The manufacture of synthetic non-sintered and degradable bone grafting substitutes
A new synthetic bone grafting substitute (NanoBone®, ARTOSS GmbH, Germany)
is presented. This is produced by a new technique, the sol-gel-method. This
bone grafting substitute consists of nanocrystalline hydroxyapatite (HA) and
nanostructured silica (SiO2). By achieving a highly porous structure good osteoconductivity
can be seen. In addition, the material will be completely biodegraded
and new own bone is formed. It has been demonstrated that NanoBone® is
biodegraded by osteoclasts in a manner comparable to the natural bone remodelling
process
High-excitation OH and H_2O lines in Markarian 231: the molecular signatures of compact far-infrared continuum sources
The ISO/LWS far-infrared spectrum of the ultraluminous galaxy Mkn 231 shows
OH and H_2O lines in absorption from energy levels up to 300 K above the ground
state, and emission in the [O I] 63 micron and [C II] 158 micron lines. Our
analysis shows that OH and H_2O are radiatively pumped by the far-infrared
continuum emission of the galaxy. The absorptions in the high-excitation lines
require high far-infrared radiation densities, allowing us to constrain the
properties of the underlying continuum source. The bulk of the far-infrared
continuum arises from a warm (T_dust=70-100 K), optically thick
(tau_100micron=1-2) medium of effective diameter 200-400 pc. In our best-fit
model of total luminosity L_IR, the observed OH and H2O high-lying lines arise
from a luminous (L/L_IR~0.56) region with radius ~100 pc. The high surface
brightness of this component suggests that its infrared emission is dominated
by the AGN. The derived column densities N(OH)>~10^{17} cm^{-2} and
N(H_2O)>~6x10^{16} cm^{-2} may indicate XDR chemistry, although significant
starburst chemistry cannot be ruled out. The lower-lying OH, [C II] 158 micron,
and [O I] 63 micron lines arise from a more extended (~350 pc) starburst
region. We show that the [C II] deficit in Mkn 231 is compatible with a high
average abundance of C+ because of an extreme overall luminosity to gas mass
ratio. Therefore, a [C II] deficit may indicate a significant contribution to
the luminosity by an AGN, and/or by extremely efficient star formation.Comment: 16 pages, 6 figures, accepted for publication in The Astrophysical
Journa
Infrared Dark Clouds in the Small Magellanic Cloud?
We have applied the unsharp-masking technique to the 24 m image of the
Small Magellanic Cloud (SMC), obtained with the Spitzer Space Telescope, to
search for high-extinction regions. This technique has been used to locate very
dense and cold interstellar clouds in the Galaxy, particularly infrared dark
clouds (IRDCs). Fifty five candidate regions of high-extinction, namely
high-contrast regions (HCRs), have been identified from the generated
decremental contrast image of the SMC. Most HCRs are located in the southern
bar region and mainly distributed in the outskirts of CO clouds, but most
likely contain a significant amount of H2. HCRs have a peak-contrast at 24
m of 2 - 2.5 % and a size of 8 - 14 pc. This corresponds to the size of
typical and large Galactic IRDCs, but Galactic IRDCs are 2 - 3 times darker at
24 m than our HCRs. To constrain the physical properties of the HCRs, we
have performed NH3, N2H+, HNC, HCO+, and HCN observations toward one of the
HCRs, HCR LIRS36-EAST, using the Australia Telescope Compact Array and the
Mopra single-dish radio telescope. We did not detect any molecular line
emission, however, our upper limits to the column densities of molecular
species suggest that HCRs are most likely moderately dense with n ~ 10^{3}
cm-3. This volume density is in agreement with predictions for the cool atomic
phase in low metallicity environments. We suggest that HCRs may be tracing
clouds at the transition from atomic to molecule-dominated medium, and could be
a powerful way to study early stages of gas condensation in low metallicity
galaxies. Alternatively, if made up of dense molecular clumps < 0.5 pc in size,
HCRs could be counterparts of Galactic IRDCs, and/or regions with highly
unusual abundance of very small dust grains.Comment: accepted for publication in the Astronomical Journa
First-Order Phase Transition with Breaking of Lattice Rotation Symmetry in Continuous-Spin Model on Triangular Lattice
Using a Monte Carlo method, we study the finite-temperature phase transition
in the two-dimensional classical Heisenberg model on a triangular lattice with
or without easy-plane anisotropy. The model takes account of competing
interactions: a ferromagnetic nearest-neighbor interaction and an
antiferromagnetic third nearest-neighbor interaction . As a result, the
ground state is a spiral spin configuration for . In this
structure, global spin rotation cannot compensate for the effect of 120-degree
lattice rotation, in contrast to the conventional 120-degree structure of the
nearest-neighbor interaction model. We find that this model exhibits a
first-order phase transition with breaking of the lattice rotation symmetry at
a finite temperature. The transition is characterized as a vortex
dissociation in the isotropic case, whereas it can be viewed as a vortex
dissociation in the anisotropic case. Remarkably, the latter is continuously
connected to the former as the magnitude of anisotropy decreases, in contrast
to the recent work by Misawa and Motome [J. Phys. Soc. Jpn. \textbf{79} (2010)
073001.] in which both the transitions were found to be continuous.Comment: 11pages, 16figures, accepted to JPS
Phenotypic and molecular characterization of Staphylococcus aureus isolates expressing low- and high-level mupirocin resistance in Nigeria and South Africa
<p>Abstract</p> <p>Background</p> <p>Mupirocin is a topical antimicrobial agent which is used for the treatment of skin and postoperative wound infections, and the prevention of nasal carriage of methicillin-resistant <it>Staphylococcus aureus </it>(MRSA). However, the prevalence of mupirocin resistance in <it>S. aureus</it>, particularly in MRSA, has increased with the extensive and widespread use of this agent in hospital settings. This study characterized low- and high-level mupirocin-resistant <it>S. aureus </it>isolates obtained from Nigeria and South Africa.</p> <p>Methods</p> <p>A total of 17 mupirocin-resistant <it>S. aureus </it>isolates obtained from two previous studies in Nigeria and South Africa, were characterized by antibiogram, PCR-RFLP of the coagulase gene and PFGE. High-level mupirocin resistant isolates were confirmed by PCR detection of the <it>mupA </it>gene. The genetic location of the resistance determinants was established by curing and transfer experiments.</p> <p>Results</p> <p>All the low-level mupirocin resistant isolates were MRSA and resistant to gentamicin, tetracycline and trimethoprim. PFGE identified a major clone in two health care institutions located in Durban and a health care facility in Pietermaritzburg, Greytown and Empangeni. Curing and transfer experiments indicated that high-level mupirocin resistance was located on a 41.1 kb plasmid in the South African strain (A15). Furthermore, the transfer of high-level mupirocin resistance was demonstrated by the conjugative transfer of the 41.1 kb plasmid alone or with the co-transfer of a plasmid encoding resistance to cadmium. The size of the mupirocin-resistance encoding plasmid in the Nigerian strain (35 IBA) was approximately 35 kb.</p> <p>Conclusion</p> <p>The emergence of mupirocin-resistant <it>S. aureus </it>isolates in Nigeria and South Africa should be of great concern to medical personnel in these countries. It is recommended that methicillin-susceptible <it>S. aureus </it>(MSSA) and MRSA should be routinely tested for mupirocin resistance even in facilities where the agent is not administered. Urgent measures, including judicious use of mupirocin, need to be taken to prevent clonal dissemination of the mupirocin/methicillin resistant <it>S. aureus </it>in KZN, South Africa and the transfer of the conjugative plasmid encoding high-level mupirocin resistance identified in this study.</p
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
Abstract
BACKGROUND:
Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation.
METHODS:
Protein maps were obtained by 2-dimensional (2-D) separations consisting of isoelectrofocusing (IEF) from pI 3 to 11 followed by SDS-polyacrylamide gel electrophoresis. 2-D gels were stained with Sypro Ruby. Map images of DC and SU spermatozoa were compared using dedicated software. Intensities of a given spot were considered different between DC and SU when their group mean differed by >1.5-fold (p<0.05, Anova).
RESULTS:
No differences were observed for 853 spots, indicating a 98.7% similarity between DC and SU. Five spots were DC>SU and 1 was SU>DC. Proteins present in 3 of the differential spots could be identified. One DC>SU spot contained lactate dehydrogenase C and gamma-glutamylhydrolase, a second DC>SU spot contained fumarate hydratase and glyceraldehyde-3-phosphate dehydrogenase-2, and a SU>DC spot contained pyruvate kinase M1/M2.
CONCLUSIONS:
The differences in protein levels found on comparison of DC with SU spermatozoa indicate possible dissimilarities in their glycolytic metabolism and DNA methylation and suggest that DC cells may have a better capacitation potential
Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells
Protection from ultraviolet damage and photocarcinogenesis by vitamin d compounds
© Springer Nature Switzerland AG 2020. Exposure of skin cells to UV radiation results in DNA damage, which if inadequately repaired, may cause mutations. UV-induced DNA damage and reactive oxygen and nitrogen species also cause local and systemic suppression of the adaptive immune system. Together, these changes underpin the development of skin tumours. The hormone derived from vitamin D, calcitriol (1,25-dihydroxyvitamin D3) and other related compounds, working via the vitamin D receptor and at least in part through endoplasmic reticulum protein 57 (ERp57), reduce cyclobutane pyrimidine dimers and oxidative DNA damage in keratinocytes and other skin cell types after UV. Calcitriol and related compounds enhance DNA repair in keratinocytes, in part through decreased reactive oxygen species, increased p53 expression and/or activation, increased repair proteins and increased energy availability in the cell when calcitriol is present after UV exposure. There is mitochondrial damage in keratinocytes after UV. In the presence of calcitriol, but not vehicle, glycolysis is increased after UV, along with increased energy-conserving autophagy and changes consistent with enhanced mitophagy. Reduced DNA damage and reduced ROS/RNS should help reduce UV-induced immune suppression. Reduced UV immune suppression is observed after topical treatment with calcitriol and related compounds in hairless mice. These protective effects of calcitriol and related compounds presumably contribute to the observed reduction in skin tumour formation in mice after chronic exposure to UV followed by topical post-irradiation treatment with calcitriol and some, though not all, related compounds
Varying constants, Gravitation and Cosmology
Fundamental constants are a cornerstone of our physical laws. Any constant
varying in space and/or time would reflect the existence of an almost massless
field that couples to matter. This will induce a violation of the universality
of free fall. It is thus of utmost importance for our understanding of gravity
and of the domain of validity of general relativity to test for their
constancy. We thus detail the relations between the constants, the tests of the
local position invariance and of the universality of free fall. We then review
the main experimental and observational constraints that have been obtained
from atomic clocks, the Oklo phenomenon, Solar system observations, meteorites
dating, quasar absorption spectra, stellar physics, pulsar timing, the cosmic
microwave background and big bang nucleosynthesis. At each step we describe the
basics of each system, its dependence with respect to the constants, the known
systematic effects and the most recent constraints that have been obtained. We
then describe the main theoretical frameworks in which the low-energy constants
may actually be varying and we focus on the unification mechanisms and the
relations between the variation of different constants. To finish, we discuss
the more speculative possibility of understanding their numerical values and
the apparent fine-tuning that they confront us with.Comment: 145 pages, 10 figures, Review for Living Reviews in Relativit
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