PCR amplification and sequence analysis of mole rat (Spalax leucodon) Sox2 gene

Sox2 gen je uključen u kontrolu razvića oka i kod sisara je pokazano da je transkripcioni aktivator γF-kristalina u sočivu oka. Slepo kuče (Spalax leucodon), je ekstremni primer prirodne degeneracije oka. Ova životinja nema spoljašnje oči, a sočivo je neorganizovano i vakuolizovano. U ovom radu smo prikazali parcijalnu karakterizaciju Sox2 gena slepog kučeta. Gen je kloniran korišćenjem pristupa zasnovanog na PCR tehnologiji (upotrebom 'prajmera' specifičnih za SOX2 gen čoveka). Naši rezultati ukazuju da u otvorenom okviru čitanja Sox2 gena kod slepog kučeta nema značajnih promena u odnosu na do sada okarakterisane Sox2 gene kod sisara. Zapaža se snažna konzervisanost sekvence u poređenju sa ortolognim genima čoveka, ovce i miša. Naši rezultati ukazuju da je Sox2 gen slepog kučeta pod selektivnim pritiskom, što ukazuje da sem u razviću oka verovatno ima i druge važne funkcije u embriogenezi.Sox2 gene is involved in control of eye development and it has been shown that in mammals this gene is a transcriptional activator of γF-crystallin in lens. The mole rat (Spalax leucodon), which is an extreme example of natural visual degeneration, has strongly rudimented eyes with undeveloped, disorganized and vacuolated lens nuclei. Here we present a partial characterization of the mole rat Sox2 gene which is cloned using PCR - based approach (and primers specific for human SOX2 gene). Our results have shown no significant changes in the sequence within the open reading frame of the mole rat Sox2 gene and have revealed that mole rat Sox2 gene is strongly conserved compared with orthologue gene in humans, sheep and mouse. Our results indicate that the mole rat Sox2 gene remains under selective pressure since it probably has, apart from the control of eye development, the other important roles in the embryonic development

Urbana obnova u Slavonskom brodu nakon Drugog svjetskog rata

U diplomskom radu prikazana je urbana obnova Slavonskog Broda nakon Drugog svjetskog rata s naglaskom na glavni gradski trg, Šetalište braće Radića, Ulicu Ante Starčevića, Ulicu Matije Mesića, Ulicu Petra Krešimira IV. te Trg pobjede. Rad sadrži kratki pregled povijesti grada i njegovog urbanističkog razvoja od osnutka do početka Drugog svjetskog rata te definiciju urbane obnove kao i njezinih metoda. Obnovom su obuhvaćene najreprezentativnije građevine, uz prikaz njihovog prošlog i sadašnjeg stanja. Zaključno poglavlje daje kritiku današnjeg društva, koje je izgubilo osjećaj brige za građevine od povijesnoumjetničkog značaja.This master’s thesis highlights the urban renewal of Slavonski Brod after the Second World War. The special emphasis is put on the main town square, Šetalište braće Radića, Ulica Ante Starčevića, Ulica Matije Mesića, Ulica Petra Krešimira IV and Trg pobjede. The paper offers a brief overview of town’s history and urban development from its founding to the beginning of the Second World War. It also includes the definition of urban renewal and the description of its methods. The renewal encompasses all the most reputable buildings, showing their past and their present condition. The closing chapter is a critique of today's society, which has lost the sense of concern for the buildings of major historical and artistic significance

Urbana obnova u Slavonskom brodu nakon Drugog svjetskog rata

U diplomskom radu prikazana je urbana obnova Slavonskog Broda nakon Drugog svjetskog rata s naglaskom na glavni gradski trg, Šetalište braće Radića, Ulicu Ante Starčevića, Ulicu Matije Mesića, Ulicu Petra Krešimira IV. te Trg pobjede. Rad sadrži kratki pregled povijesti grada i njegovog urbanističkog razvoja od osnutka do početka Drugog svjetskog rata te definiciju urbane obnove kao i njezinih metoda. Obnovom su obuhvaćene najreprezentativnije građevine, uz prikaz njihovog prošlog i sadašnjeg stanja. Zaključno poglavlje daje kritiku današnjeg društva, koje je izgubilo osjećaj brige za građevine od povijesnoumjetničkog značaja.This master’s thesis highlights the urban renewal of Slavonski Brod after the Second World War. The special emphasis is put on the main town square, Šetalište braće Radića, Ulica Ante Starčevića, Ulica Matije Mesića, Ulica Petra Krešimira IV and Trg pobjede. The paper offers a brief overview of town’s history and urban development from its founding to the beginning of the Second World War. It also includes the definition of urban renewal and the description of its methods. The renewal encompasses all the most reputable buildings, showing their past and their present condition. The closing chapter is a critique of today's society, which has lost the sense of concern for the buildings of major historical and artistic significance

A Critique of Ideological Identity Construction in Socialist Yugoslavia: Music Video Clips "Venceremos (133)" and "Discipline" by Borghesia

U ’pukotini’ socijalističke ideologije Jugoslavije, tijekom 80-ih godina, pojavio se ’prostor’ za novoideološku konstrukciju, koja je samo naizgled djelovala kao ’slobodna tvorevina’ alternativnih mišljenja i izražavanja u kasnom socijalizmu. Pravi kasnosocijalistički alternativni umjetnički i kulturalni istupi bili su usmjereni prema preispitivanju i kritiziranju ideološke konstrukcije socijalizma, a onda i kulturalnih identifikacijskih upisa. Glasnogovornik na alternativnoj sceni kasnosocijalističke Jugoslavije, grupa Borghesia iz Slovenije – u ’tekstovima’ muzičkih videoradova Venceremos (133) i Discipline – zanijekala je upise nacionalnih identiteta u socijalističkoj ideologiji. Oni su anticipirali novu homogenizaciju, odnosno interpelaciju individue u kolektivni identitet postsocijalističke nacionalne ideologije.During the 1980s, some “room” appeared in the “gap” of Yugoslav socialist ideology for a new ideological construction, which was only apparently a “free product” of alternative modes of thinking and expression in late socialism. Genuinely alternative artistic and cultural actions in this late socialist period focused on the analysis and critique of socialist ideological construct, as well as its cultural production of identity. One of such spokesmen on the alternative scene of late socialist Yugoslavia, the Slovenian music band Borghesia, denounced these productions of national identity within socialist ideology in its “texts” for the music videos Venceremos (133) and Discipline. Thus, Borghesia anticipated the new homogenization and interpellation of the individual in the collective identity of postsocialist national ideology

On the reduction of Linear Parameter-Varying State-Space models

This paper presents an overview and comparative study of the state of the art in State-Order Reduction (SOR) and Scheduling Dimension Reduction (SDR) for Linear Parameter-Varying (LPV) State-Space (SS) models, comparing and benchmarking their capabilities, limitations and performance. The use case chosen for these studies is an interconnected network of nonlinear coupled mass spring damper systems with three different configurations, where some spring coefficients are described by arbitrary user-defined static nonlinear functions. For SOR, the following methods are compared: Linear Time-Invariant (LTI), LPV and LFR-based balanced reductions, moment matching and parameter-varying oblique projection. For SDR, the following methods are compared: Principal Component Analysis (PCA), trajectory PCA, Kernel PCA and LTI balanced truncation, autoencoders and deep neural network. The comparison reveals the most suitable reduction methods for the different benchmark configurations, from which we provide use case SOR and SDR guidelines that can be used to choose the best reduction method for a given LPV-SS model

Color estructural: un nuevo campo de exploración para la ciencia y el diseño

El color observado en la naturaleza se obtiene de dos formas: por pigmentación o por estructura o color estructural.&nbsp

Evidence of genetic isolation between two Mediterranean morphotypes of Parazoanthus axinellae

Coralligenous assemblages are among the most species-rich and vulnerable habitats of the Mediterranean Sea. Nevertheless, data on connectivity patterns on species inhabiting these habitats, crucial to define management and protection priorities, are largely lacking. Moreover, unreliable species-level taxonomy can confound ecological studies and mislead management strategies. In the northwestern Mediterranean two Parazoanthus axinellae morphotypes differing in size, color and preferred substrate are found in sympatry. In this study, we used COI and ITS sequence polymorphism to assess (1) the genetic divergence between the two morphotypes, (2) their connectivity patterns and (3) their phylogenetic position within the Parazoanthidae. Specimens of P. axinellae were sampled in 11 locations along the northwestern Mediterranean; in 6 locations, samples of the two morphotypes were collected in sympatry. Small genetic diversity and structure were found within morphotypes, while marked and consistent differentiation was detected between them. Moreover, the less widespread morphotype appeared to be closer to Pacific species as P. juanfernandezii and P. elongatus. Our findings confirmed the limited knowledge on Parazoanthus species complex, and how this gap can have important implication for the conservation strategies of this widespread and valuable genus in the Mediterranean Sea

On the reduction of Linear Parameter-Varying State-Space models

This paper presents an overview and comparative study of the state of the art in State-Order Reduction (SOR) and Scheduling Dimension Reduction (SDR) for Linear Parameter-Varying (LPV) State-Space (SS) models, comparing and benchmarking their capabilities, limitations and performance. The use case chosen for these studies is an interconnected network of nonlinear coupled mass spring damper systems with three different configurations, where some spring coefficients are described by arbitrary user-defined static nonlinear functions. For SOR, the following methods are compared: Linear Time-Invariant (LTI), LPV and LFR-based balanced reductions, moment matching and parameter-varying oblique projection. For SDR, the following methods are compared: Principal Component Analysis (PCA), trajectory PCA, Kernel PCA and LTI balanced truncation, autoencoders and deep neural network. The comparison reveals the most suitable reduction methods for the different benchmark configurations, from which we provide use case SOR and SDR guidelines that can be used to choose the best reduction method for a given LPV-SS model

Alteracija lokomotorne aktivnosti (LA) miševa otrovanih subletalnim dozama olovnog acetata

It was shown that locomotometry allows for continuous recording of some injurious effects of lead poisoning upon mice. For detection of poisoning with lead acetate in mice, locomotometry was more sensitive than the recording of mortality rates, demonstration of stippled cell, in the peripheral blood or detection of manifest symptoms of illness by plain inspection. Single or multiple intraperitoneal injections of saline or distilled water, after the third post-injection day, do not induce detectable alterations of the locomotive activity (LA) of mice. Dose-dependent alterations of LA have been observed after intraperitoneal administration of lead acetate. Acute, single-dose poisoning induces dose-dependent depressions of the LA, while chronic poisoning with multiple injections tends to induce elevated LA, mostly combined with and exceptionally without depression of the LA. Time trends show intermittent alterations of the LA induced by lead poisoning. In some animals significant alterations of LA lasted as long as observed (from 4 to 6 months), while in others the normal LA was restored within 20 or 60 days after the last injection of lead acetate. Animals poisoned with lethal doses of lead acetate developed stippled cells during the 2nd or 3rd week, while significant alterations of the LA started to be manifest from the first day after the first injection. Mice poisoned with subletal doses {0.4, 0.8 and 1.0 mg) showed no stippled cells, but displayed alterations of LA. Possible applications of locomotometry for continuous measurements of degrees of illness or of clinical improvements, in cases of poisoning or of various treatments, were discussed briefly.Metod lokomotometrije omogućuje kontinuirano registrovanje patološkog dejstva otrovanja olovom na miševe. Za detekciju otrovanja olovom metoda lokomotometrije pokazala se je znatno osetljivijom od praćenja mortaliteta, od nalaza bazofilnih granulacija u eritrocitima, i od registrovanja manifestnih simptoma oboljenja. Od trećeg dana posle aplikacije jedne ili više intraperatonealnih injekcija vode, LA miševa ne ispoljava merljive alteracije. Intraperitonealne injekcije rastvora olovnog acetata izazivaju alteraciju LA, koja je proporcionalna dozi unetog otrova. Akutna otrovanja, izazvana pojedinačnim dozama izazivaju usporavanje pokretljivosti, dok je u slučajevima hroničnih otrovanja, izazvanih multiplim dozama olova, zapažene i povišenje LA miševa. U nekih životinja zapažane su mesecima značajne, većinom intermibirajuće, aliteracije LA, dok je u drugih LA normalizovana u roku od 20 do 60 dana posle poslednje injekcije olovnog acetata. Bazofilne granulacije eritrocita pojavljuju se tek oko desetog dana posle otrovanja letalnim dozama olova, dok je značajna alteraoija LA zapažena već prvih dana posle davanja otrova. U eritrocitima miševa otrovanih subletainim dozama otrova bazofilne granulacije nisu nađene. Diskutovane su mogućnosti primene lokomotometrije u cilju kontinuiranog merenja jačine bolesti usled otrovanja ili u cilju ocene kliničkog poboljšanja pod dejstvom raznih tretmana