Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection

Arsenite is an inorganic form of arsenic that poses hazardous effect to human. It is a common environmental heavy metal contaminant ubiquitously found in water and groundwater. In this study, an optical biosensor for arsenite determination was developed by immobilization of crude arsenite oxidase (Aio) extracted from recombinant E. coli, in chitosan solution coated on triacetyl-cellulose membrane employing DCPIP as colour indicator. The arsenite oxidase (Aio) was successfully expressed and extracted from recombinant E. coli strain BL21 (DE3). The protein concentration and specific activity of the crude arsenite oxidase were determined. Expression of Aio was confirmed by SDS-PAGE. The crude Aio was also successfully immobilized in chitosan and coated on triacetyl cellulose membrane. The response time and dynamic range of the optical biosensor were optimized. The response time of the developed biosensor was 15 minutes. The amount of DCPIP reduced (ΔA) was inversely proportional to the arsenite concentration. Standard calibration curve for arsenite detection was achieved within the range of arsenite concentration from 25 μM to 200 μM. The maximum detection limit was determined to be 250 μM arsenite

Beyond ‘greeting’ and ‘thanking’: politeness in job interviews

Despite the steps taken by Malaysian institutions of higher learning to equip their graduates with the necessary communication skills, local graduates are still failing to create a positive impression on the employers especially during job interviews. Hence this study explored the face-related concept of politeness proposed by Brown and Levinson (1999) at job interviews. Eight final-year undergraduates taking a communication for employment course volunteered for a mock-interview session with a human resource manager from the banking industry. The interviews were audio- and video-recorded and subsequently transcribed. Then a micro-analysis of the data was carried out using Brown and Levinson’s (1999) framework to identify the politeness strategies that the candidates used. Two positive politeness strategies were identified, the first is noticing and attending to interviewer’s interests, wants, needs or goods, and second is raising/asserting common grounds to establish solidarity. One negative politeness strategy was identified which is requesting for clarification or repetition. The pedagogical implications of these findings will be discussed and recommendations for teaching face-related politeness in communication for employment courses will be offered

A survey on touch dynamics authentication in mobile devices

© 2016 Elsevier Ltd. All rights reserved. There have been research activities in the area of keystroke dynamics biometrics on physical keyboards (desktop computers or conventional mobile phones) undertaken in the past three decades. However, in terms of touch dynamics biometrics on virtual keyboards (modern touchscreen mobile devices), there has been little published work. Particularly, there is a lack of an extensive survey and evaluation of the methodologies adopted in the area. Owing to the widespread use of touchscreen mobile devices, it is necessary for us to examine the techniques and their effectiveness in the domain of touch dynamics biometrics. The aim of this paper is to provide some insights and comparative analysis of the current state of the art in the topic area, including data acquisition protocols, feature data representations, decision making techniques, as well as experimental settings and evaluations. With such a survey, we can gain a better understanding of the current state of the art, thus identifying challenging issues and knowledge gaps for further research

The cost of work-related stress to society: a systematic review

Objective. A global and systematic review of the available evidence examining the cost of work-related stress would yield important insights into the magnitude and nature of this social phenomenon. The objective of this systematic review was to collate, extract, review, and synthesize economic evaluations of the cost of work-related stress to society. Method. A research protocol was developed outlining the search strategy. Included cost-of-illness (COI) studies estimated the cost of work-related stress at a societal level, and were published in English, French or German. Searches were carried out in ingenta connect, EBSCO, JSTOR, Science Direct, Web of Knowledge, Google and Google scholar. Included studies were assessed against ten COI quality assessment criteria. Results. Fifteen COI studies met the inclusion criteria and were reviewed. These originated from Australia, Canada, Denmark, France, Sweden, Switzerland, the United Kingdom, the United States, and the EU-15. At a national and pan-European level, the total estimated cost of work-related stress in 2014 was observed to be considerable and ranged substantially from US $221.13 million to$187 billion. Productivity related losses were observed to proportionally contribute the majority of the total cost of work-related stress (between 70 to 90%), with healthcare and medical costs constituting the remaining 10% to 30%. Conclusion. The evidence reviewed here suggests a sizeable financial burden imposed by work-related stress on society. The observed range of cost estimates across studies was understood to be attributable to variations in definitions of work-related stress; the number and type of costs estimated; and, in how production loss was estimated. It is postulated that the cost estimates identified by this review are likely conservative due to narrow definitions of work - related stress (WRS) and the exclusion of diverse range of cost components

Murine HPV16 E7-expressing transgenic skin effectively emulates the cellular and molecular features of human high-grade squamous intraepithelial lesions.

Currently available vaccines prevent HPV infection and development of HPV-associated malignancies, but do not cure existing HPV infections and dysplastic lesions. Persistence of infection(s) in immunocompetent patients may reflect induction of local immunosuppressive mechanisms by HPV, providing a target for therapeutic intervention. We have proposed that a mouse, expressing HPV16 E7 oncoprotein under a Keratin 14 promoter (K14E7 mice), and which develops epithelial hyperplasia, may assist with understanding local immune suppression mechanisms that support persistence of HPV oncogene-induced epithelial hyperplasia. K14E7 skin grafts recruit immune cells from immunocompetent hosts, but consistently fail to be rejected. Here, we review the literature on HPV-associated local immunoregulation, and compare the findings with published observations on the K14E7 transgenic murine model, including comparison of the transcriptome of human HPV-infected pre-malignancies with that of murine K14E7 transgenic skin. We argue from the similarity of i) the literature findings and ii) the transcriptome profiles that murine K14E7 transgenic skin recapitulates the cellular and secreted protein profiles of high-grade HPV-associated lesions in human subjects. We propose that the K14E7 mouse may be an appropriate model to further study the immunoregulatory effects of HPV E7 expression, and can facilitate development and testing of therapeutic vaccines

E-readiness to G-readiness: developing a green information technology readiness framework

Businesses are under increasing pressure from competitors, regulators and community groups to implement sustainable business practices. Balancing economic and environmental performance to be green and competitive is therefore a key strategic issue. The increased discussion on green information technology (IT) has sparked the interest of this research. Green IT is poised to influence not only technology but also competitive strategy and even the legality of some business strategic options. Understanding and leveraging Green IT is therefore critical for businesses¿ continued progress. Nevertheless, the principles, practices and value of Green IT is yet to be researched. This paper introduces the concept of Green IT and describes the main pillars of a g-readiness framework to help organisations evaluate their readiness for adopting Green IT. It argues that just as e-readiness has been, and continues to be, a critical quality in the digital economy, g-readiness is an equally critical quality in the low carbon digital economy. Without a clear understanding of g-readiness, organisations would approach Green IT initiatives on an ad hoc and somewhat reactive basis which is undesirable

Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes

10.1186/1476-8518-6-2Journal of Immune Based Therapies and Vaccines6

The interaction between supportive and unsupportive manager behaviors on employee work attitudes

Purpose: To use Social Exchange Theory (SET) to examine a model where supportive (SMB) and unsupportive (UMB) manager behaviors interact to predict employees’ engagement, job satisfaction and turnover intention. Design/Methodology: A cross-sectional online survey collected data from 252 UK based employees of a global data management company. Findings: Factor analysis confirmed manager behaviors to consist of two constructs: supportive and unsupportive behaviors. Structural equation modelling indicated SMB predicted job satisfaction and turnover intentions, but not engagement. Job satisfaction, but not engagement, mediated the SMB-turnover intention relationship. UMB only predicted job dissatisfaction. Neither job satisfaction nor engagement mediated the UMB-turnover intention relationship. UMB undermined the positive relationship between SMB and turnover intention. Implications: The behaviors assessed can be integrated into various stages of a manager’s development process to serve as guidelines of good practice. Crucially, findings suggest managers can exhibit both supportive and unsupportive behaviors, and that consistency in behaviors is important. The study also provides evidence that supportive managers can help reduce turnover intention through job satisfaction. Originality/value: SET was used as a framework for SMB, UMB and engagement. To our knowledge this is the first study to examine the interaction between SMB and UMB

Evolution of precision agriculture computing towards sustainable oil palm industry

Precision technology elements have not been implemented yet into the sustainable oil palm industry because the knowledge and technology gap. To resolve the gaps, promote sustainability and integrate the technologies, Oil Palm Management System (OPAMS) was introduced. The precision technologies in OPAMS comprises of Geographical Information System (GIS), Global Positioning System (GPS), remote sensing and yield monitoring. A phase by phase System Development Life Cycle (SDLC) methodology was used to generate the said system with feedbacks from oil palm planters as the inputs for OPAMS’s key features. OPAMS ultimately aims to increase the awareness of the industry on the benefits of utilizing technology to improve plantation performances, increase business and environmental sustainability