Redox kinetics of the amyloid-β-Cu complex and its biological implications

The ability of the amyloid-β peptide to bind to redox active metals and act as a source of radical damage in Alzheimer’s disease has been largely accepted as contributing to the disease’s pathogenesis. However, a kinetic understanding of the molecular mechanism, which underpins this radical generation, has yet to be reported. Here we use a sensitive fluorescence approach, which reports on the oxidation state of the metal bound to the amyloid-β peptide and can therefore shed light on the redox kinetics. We confirm that the redox goes via a low populated, reactive intermediate and that the reaction proceeds via the Component I coordination environment rather than Component II. We also show that while the reduction step readily occurs (on the 10 ms time scale) it is the oxidation step that is rate-limiting for redox cycling

Whole-system assessment of the benefits of integrated electricity and heat system

The interaction between electricity and heat systems will play an important role in facilitating the cost effective transition to a low carbon energy system with high penetration of renewable generation. This paper presents a novel integrated electricity and heat system model in which, for the first time, operation and investment timescales are considered while covering both the local district and national level infrastructures. This model is applied to optimize decarbonization strategies of the UK integrated electricity and heat system, while quantifying the benefits of the interactions across the whole multi-energy system, and revealing the trade-offs between portfolios of (a) low carbon generation technologies (renewable energy, nuclear, CCS) and (b) district heating systems based on heat networks (HN) and distributed heating based on end-use heating technologies. Overall, the proposed modeling demonstrates that the integration of the heat and electricity system (when compared with the decoupled approach) can bring significant benefits by increasing the investment in the heating infrastructure in order to enhance the system flexibility that in turn can deliver larger cost savings in the electricity system, thus meeting the carbon target at a lower whole-system cost

Tests on RC Beams Strengthened at the Span with Externally Bonded Polymers Reinforced with Carbon or Steel Fibers

The main objective of the experimental work reported herein is the comparative evaluation of steel-reinforced polymers (SRPs) and carbon-reinforced polymers (CFRPs) used as externally-bonded reinforcement in strengthening of reinforced-concrete (RC) members. Tensile stress strain as well as bond constitutive laws for these materials were first derived from 16 tests and are summarized here. Results are then reported from four-point bending tests of five full-scale RC beams strengthened at their span using SRP and CFRP strips. The bond tests have shown that by providing a bond length greater than the effective one, neither the bond strength nor the deformation capacity are increased, whereas by increasing the width of the strip the bond strength is increased. From the bending tests of beams it was found that the use of both SRP and CFRP strips resulted in a significant increase in strength (up to 92%) with respect to the strength of the initial specimen. The experimentally measured strengths were estimated analytically using both the experimental measurements of the specimen deformations and the pertinent provisions of standards from the American Concrete Institute and the European Committee for Standardization

Interactive boundary-layer method for unsteady airfoil flows - Quasisteady model

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/76445/1/AIAA-25340-880.pd

Quantitative localized proton-promoted dissolution kinetics of calcite using scanning electrochemical microscopy (SECM)

Scanning electrochemical microscopy (SECM) has been used to determine quantitatively the kinetics of proton-promoted dissolution of the calcite (101̅4) cleavage surface (from natural “Iceland Spar”) at the microscopic scale. By working under conditions where the probe size is much less than the characteristic dislocation spacing (as revealed from etching), it has been possible to measure kinetics mainly in regions of the surface which are free from dislocations, for the first time. To clearly reveal the locations of measurements, studies focused on cleaved “mirror” surfaces, where one of the two faces produced by cleavage was etched freely to reveal defects intersecting the surface, while the other (mirror) face was etched locally (and quantitatively) using SECM to generate high proton fluxes with a 25 μm diameter Pt disk ultramicroelectrode (UME) positioned at a defined (known) distance from a crystal surface. The etch pits formed at various etch times were measured using white light interferometry to ascertain pit dimensions. To determine quantitative dissolution kinetics, a moving boundary finite element model was formulated in which experimental time-dependent pit expansion data formed the input for simulations, from which solution and interfacial concentrations of key chemical species, and interfacial fluxes, could then be determined and visualized. This novel analysis allowed the rate constant for proton attack on calcite, and the order of the reaction with respect to the interfacial proton concentration, to be determined unambiguously. The process was found to be first order in terms of interfacial proton concentration with a rate constant k = 6.3 (± 1.3) × 10–4 m s–1. Significantly, this value is similar to previous macroscopic rate measurements of calcite dissolution which averaged over large areas and many dislocation sites, and where such sites provided a continuous source of steps for dissolution. Since the local measurements reported herein are mainly made in regions without dislocations, this study demonstrates that dislocations and steps that arise from such sites are not needed for fast proton-promoted calcite dissolution. Other sites, such as point defects, which are naturally abundant in calcite, are likely to be key reaction sites

Quark Delocalization, Color Screening, and Nuclear Intermediate Range Attraction

We consider the effect of including quark delocalization and color screening, in the nonrelativistic quark cluster model, on baryon-baryon potentials and phase shifts. We find that the inclusion of these additional effects allows a good qualitative description of both.Comment: 10 pages, LaTeX, 4 figures in PostScript after text, LA-UR-91-215

Search for Intrinsic Excitations in 152Sm

The 685 keV excitation energy of the first excited 0+ state in 152Sm makes it an attractive candidate to explore expected two-phonon excitations at low energy. Multiple-step Coulomb excitation and inelastic neutron scattering studies of 152Sm are used to probe the E2 collectivity of excited 0+ states in this "soft" nucleus and the results are compared with model predictions. No candidates for two-phonon K=0+ quadrupole vibrational states are found. A 2+, K=2 state with strong E2 decay to the first excited K=0+ band and a probable 3+ band member are established.Comment: 4 pages, 6 figures, accepted for publication as a Rapid Communication in Physical Review

Electron irradiation induced nanocrystal formation in Cu-borosilicate glass

Nanoscale writing of Cu nanoparticles in glasses is introduced using focused electron irradiation by transmission electron microscopy. Two types of copper borosilicate glasses, one with high and another with low Cu loading, have been tested at energies of 200–300 keV, and formation of Cu nanoparticles in a variety of shapes and sizes using different irradiation conditions is achieved. Electron energy loss spectroscopy analysis, combined with high-resolution transmission electron microscopy imaging, confirmed the irradiation-induced precipitated nanoparticles as metallic, while furnace annealing of the glass triggered dendrite-shaped particles of copper oxide. Unusual patterns of nanoparticle rings and chains under focused electron beam irradiation are also presented. Conclusively, electron beam patterning of Cu-loaded glasses is a promising alternative route to well-established femtosecond laser photoreduction of Cu ions in glass

XMM-Newton Detection of a Compton-thick AGN in the 1-Jy ULIRG/LINER F04103-2838

We report on the detection of Fe Kalpha emission in F04103$-$2838, an ultraluminous infrared galaxy (ULIRG; log[L$_{\rm IR}$/L$_\odot$] $\ge$ 12) that is optically classified as a LINER. Previous {\it Chandra} observations suggested the presence of both a starburst and an AGN in this source. A deeper ($\sim$20 ksec) {\it XMM-Newton} spectrum reveals an Fe Kalpha line at rest frame energy $\sim$6.4 keV, consistent with cold neutral iron. The best-fit spectral model indicates the Fe Kalpha line has an equivalent width of $\sim$1.6 keV. The hard X-ray emission is dominated by a Compton-thick AGN with intrinsic 0.2--10 keV luminosity $\sim10^{44}$ ergs s$^{-1}$, while the soft X-ray emission is from $\sim$0.1 keV gas attributed to the starburst. The X-ray spectrum of this source bears a striking resemblance to that of the archetypal luminous infrared galaxy NGC 6240 despite differences in merger state and infrared properties.Comment: 23 pre-print pages including 5 figures and 1 table. Accepted by Ap