A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction

This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Tellez, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentin

KINEMATICS OF THORAX AND PELVIS DURING MAXIMAL ACCELERATED SPRINTING

Changes in thorax and pelvis movements during acceleration phase of maximal sprinting, which relate to acceleration ability, are still unknown. This study aimed to clarify the changes in thorax and pelvis movements during maximal accelerated sprinting and its relation to better acceleration ability. Twelve sprinters performed a 60-m sprint, during which 3D kinematics of the sprinters were obtained. The same patterns of motions were maintained for thorax and pelvis respectively throughout the entire acceleration phase, although phase profiles of relative movements between thorax and pelvis in three planes differed. Moreover, results indicated that effective acceleration is characterised by suppressed trunk bend, delayed trunk rotation, and forward tilted pelvis in the middle acceleration section and suppressed trunk bend in the final acceleration section

Mucosal adjuvants and delivery systems

Adjuvants comprising chitosan cross-linked with an aldehyde or mannosylated chitosan are provided herein. Methods of making the adjuvants and methods of combining or linking the adjuvants with antigens are also provided. The adjuvant-antigen combinations can be used in vaccine formulations and the vaccine formulations can be used in methods to vaccinate animals against the source of the antigen or to enhance the immune response in a subject

Regulatory review and analysis of European Union and Colombian Persistent Organic Pollutants in compost and its impact

Paper submitted to the International Conference of Agricultural Engineering - CIGR-AgEng 2012: Agriculture and Engineering for a Healthier Life, Valencia, Spain, 8-12 July 2012.Within the current Spanish and Colombian environmental policies related to urban waste, the use of organic waste through composting for use as fertilizer for agricultural use is promoted. There is a deficient classification of waste and pollution generated by the mixture of organic residues and therefore the presence of toxic elements or compounds such as Persistent Organic Pollutants (POPs). These compounds have bioaccumulating and biomagnifying characteristics and they move easily on air, water and fat. They have harmful effects on health and the environment, affecting food webs, food chains and can be precursors, as indicated by some studies, of infertility, cancer, obesity or diabetes (Porta, M., 2009). Therefore, and in order to meet existing regulations on POPs in urban compost in Spain and Colombia, a descriptive study was conducted to review legal texts and codes. This study concluded that there are health and environmental standards defining the maximum permissible quality limits to be met by the compost that is currently earmarked for agricultural use. That leads to an urgent need for political decision makers, researchers, scientific societies, academic and labor from different disciplines work together to regulate POPs content in the compost produced from organic waste

Cosmic Ray Physics with ACORDE at LHC

The use of large underground high-energy physics experiments, for comic ray studies, have been used, in the past, at CERN, in order to measure, precisely, the inclusive cosmic ray flux in the energy range from 2x10^10 - 2x10^12 eV. ACORDE, ALICE Cosmic Rays DEtector, will act as Level 0 cosmic ray trigger and, together with other ALICE apparatus, will provide precise information on cosmic rays with primary energies around 10^15 - 10^17 eV. This paper reviews the main detector features, the present status, commissioning and integration with other apparatus. Finally, we discuss the ACORDE-ALICE cosmic ray physics program.Comment: Contribution to the 2007 Europhysics Conference on High Energy Physics - Manchester, England 19-25 July 2007; 3 pages, 3 figure

ENTREVISTA A UN INSTITUTENSE Y UNIVERSITARIO ESEQUIEL CARRILLO TELLEZ

Entrevista al Capitán Ezequiel Carrillo Téllez, líder de la Federación de Pequeños Propietarios del Estad