876 research outputs found
The structure of the understorey of pure stand of Mimosa scabrella Bentham in mined area, in Poços de Caldas, Brazil
Foi realizado um inventário florístico-estrutural da regeneração natural de espécies arbustivo-arbóreas do sub-bosque de um plantio homogêneo de Mimosa scabrella Bentham implantado, visando à reabilitação de área minerada, em Poços de Caldas. Foram utilizadas dezenove parcelas de 50 m2 (5 m × 10 m) e amostrados os indivíduos arbustivo-arbóreos com altura igual ou superior a 30cm, tendo sido encontrados 1.946 indivíduos, pertencentes a 63 famílias botânicas. Amostras de solo foram coletadas à profundidade de 0 cm a 20 cm, em cada uma das dezenove parcelas e analisados os teores de areia, silte, argila, matéria orgânica, pH, nitrogênio, fósforo, potássio, cálcio e magnésio. Foi analisada a influência de variáveis edáficas sobre a densidade das espécies amostradas, utilizando Análise de Correspondência Canônica (“Canonical Correspondence Analysis” – CCA). Foi verificada correlação significativa entre elas a 5% de probabilidade pelo teste de Monte-Carlo. As espécies Miconia sellowiana, Miconia pepericarpa, Cestrum amictum, Alchornea triplinervia, Cordia superba e Casearia sylvestris apresentaram comportamento próximo ao indiferente em relação às variáveis edáficas estudadas, sendo que estas se destacam de forma superior em relação às demais espécies quanto aos parâmetros florístico-estruturais. Esse comportamento reforça a indicação de tais espécies, feita por NAPPO (1999), como de potencial para uso em plantios mistos e de enriquecimento em condições similares às da área estudada. A identificação e mensuração de outras variáveis ambientais e do histórico da área são peças importantes para o entendimento dos processos de dinâmica de povoamentos e, em particular, para áreas degradadas em fase de reabilitação. _______________________________________________________________________________ ABSTRACTA floristic and structural survey of the natural regeneration of shrubby and arboreal species of the understory of an established homogeneous plantation of Mimosa scabrella Bentham was corried out viewing, the reclamation of a mined area, in Poços de Caldas, Minas Gerais State, Brazil. Nineteen plots of 50 m2 (5 m × 10 m) were used and the shrubby and arboreal individuals measuring equal or above 30 cm were sampled and 1,946 individuals belonging to 63 botanic families were found. Soil samples were collected at a depth of 0 to 20 cm, in each of the 19 plots, and the contents of sand, silt,clay, organic matters, pH nitrogen, phosphorus, potassium, calcium and magnesium were analysed. The influence of the soil variables upon the density of the sampled species was analysed using the Canonical Correspondence Analysis (CCA). A significative correlation was verified among these variables at a level of 5% of probability using the Monte Carlo test. The species Miconia sellowiana, Miconia pepericarpa, Cestrum amictum, Alchornea triplinervia, Cordia superba and Casearia sylvestris presented a close to indifferent behaviour in relation to the soil variables studied, and these species largely surpassed the other species in relation to the floristic and the strutured parameters. This behaviour reinforces the indication of the species, made by NAPPO (1999), as having a potential for use in mixed and enrichment plantation in condition similar to those of the studied area. The identification and mensuration of the enviromental variables and of the history of the area are important pieces for the understanding of the process of population dynamics, and particularly, for degraded area getting to the reclamation phase
Zika virus infection, associated microcephaly, and low yellow fever vaccination coverage in Brazil: is there any causal link?
Introduction: Since the end of 2014, Zika virus (ZIKV) infection has been rapidly spreading in Brazil.
Methodology: To analyze the possible association of yellow fever vaccine with a protective effect against ZIKV-related microcephaly, the following spatial analyses were performed, using Brazilian municipalities as units: i) yellow fever vaccination coverage in Brazilian municipalities in individuals aged 15-49; ii) reported cases of microcephaly by municipality; and iii) confirmed cases of microcephaly related to ZIKV, by municipality. SaTScan software was used to identify clusters of municipalities for high risk of microcephaly.
Results: There were seven significant high risk clusters of confirmed microcephaly cases, with four of them located in the Northeast where yellow fever vaccination rates were the lowest. The clusters harbored only 2.9% of the total population of Brazil, but 15.2% of confirmed cases of microcephaly.
Conclusion: We hypothesize that pregnant women in regions with high yellow fever vaccination coverage may pose their offspring to lower risk for development of microcephaly. There is an urgent need for systematic studies to confirm the possible link between low yellow fever vaccination coverage, Zika virus infection and microcephaly
Blockade of CXCR1/2 chemokine receptors protects against brain damage in ischemic stroke in mice
OBJECTIVE: Ischemic stroke may result from transient or permanent reductions of regional cerebral blood flow. Polymorphonuclear neutrophils have been described as the earliest inflammatory cells to arrive in ischemic tissue. CXCR1/2 receptors are involved in the recruitment of these cells. However, the contribution of these chemokine receptors during transient brain ischemia in mice remains poorly understood. In this work, we investigated the effects of reparixin, an allosteric antagonist of CXCR1/2 receptors, in a model of middle cerebral artery occlusion and reperfusion in mice. METHODS: C57BL/6J male mice treated with reparixin or vehicle were subjected to a middle cerebral artery occlusion procedure 1 h after the treatment. Ninety minutes after ischemia induction, the monofilament that prevented blood flow was removed. Twenty-four hours after the reperfusion procedure, behavioral changes, including motor signs, were analyzed with the SmithKline/Harwell/lmperial College/Royal Hospital/Phenotype Assessment (SHIRPA) battery. The animals were sacrificed, and brain tissue was removed for histological and biochemical analyses. Histological sections were stained with hematoxylin and eosin, neutrophil infiltration was estimated by myeloperoxidase activity and the inflammatory cytokine IL-iβ was measured by ELISA. RESULTS: Pre-treatment with reparixin reduced the motor deficits observed in this model of ischemia and reperfusion. Myeloperoxidase activity and IL-iβ were reduced in the reparixin-treated group. Histological analysis revealed that ischemic injury was also attenuated by reparixin pre-treatment. CONCLUSIONS: Our results suggest that the blockade of the CXCR1/2 receptors by reparixin promotes neuroprotective effects by reducing the levels of polymorphonuclear infiltration in the brain and the tissue damage associated with middle cerebral artery occlusion and reperfusion
Diametric struture dynamics for tree and shrub natural regeneration understory within pure stands of Mimosa scabrella Bentham planted on a mined-out site at Poços de Caldas, Minas Gerais state
A área do Retiro-Branco, de propriedade da Companhia Geral de Minas, subsidiária da ALCOA Alumínio S.A., foi minerada para a extração de bauxita de 1978 até 1981. Em 1982/1983, essa área teve seu processo de reabilitação implementado mediante a hidrossemeadura de gramíneas e leguminosas e o plantio puro de Mimosa scabrella Bentham. Em 1997 foi realizado o primeiro inventário da regeneração natural do Retiro-Branco, aravés da implantação de 19 parcelas permanentes para caracterização inicial do processo de sucessão natural. E em 2000 foi realizado o segundo inventário dessas parcelas para caracterizar o processo de dinâmica da regeneração, o objeto deste trabalho. O processo de dinâmica da regeneração natural foi caracterizado mediante análises quantitativas e qualitativas da distribuição diamétrica. Os estudos indicaram que o monitoramento mediante inventário florestal contínuo, com parcelas permanentes setorizadas, é uma ferramenta viável para avaliações da dinâmica da regeneração natural, rumo à compreensão dos caminhos da sucessão. Foi caracterizado o processo de estratificação da regeneração natural, em que as espécies pioneiras e clímax foram as principais componentes do estrato inferior e as secundárias, as principais componentes do estrato superior. O povoamento florestal do Retiro-Branco está sobre intensa atividade de estruturação, caracterizando o estágio inicial do processo de sucessão. As espécies secundárias são as de maior dominância nas maiores classes de altura e de diâmetro, sendo as principais responsáveis pela edificação do estrato superior. A prognose da distribuição diamétrica, realizada mediante o emprego da Cadeia de Markov, foi uma ferramenta de fácil implementação e que permitiu prever o caminho do processo de sucessão para o povoamento todo e para os grupos ecológicos de espécies, ampliando o entendimento dos mecanismos que regem o comportamento interno da sucessão. _______________________________________________________________________________ ABSTRACTRetiro-Branco mine, a property of Companhia Geral de Minas, subsidiary of ALCOA Alumínio S.A., underwent bauxite extraction from 1978 to 1981. In 1982/1983 as part of its mine rehabilitation strategy, herbaceous species were hydroseeded and tree seedlings of Mimosa scabrella Bentham planted in the mining site. In 1997, 19 sample plots (50m2) were established in this area for the initial characterization of the natural regeneration process. In 2000, a second inventory was performed in the same plots to characterize natural regeneration dynamics. The process of natural regeneration dynamics was characterized through quantitative and qualitative analyses of diameter distribution. These studies demonstrated that using continuous forest inventory with a sample plot grid is a viable tool for evaluating natural regeneration dynamics. The stratification process for natural regeneration was characterized. Pioneer and climax species are the main components of the inferior stratum and the secondary ones the main component of the superior stratum. Retiro-Branco forest was found to be undergoing intense structuring activity, characterizing initial stage of succession. Secondary species are the most dominant in classes with greatest height and diameter, being the most responsible for the superior stratum construction. Diameter distribution prediction using Markov Matrix proved to be a simple way for foreseeing both the succession process of natural regeneration and future ecological groupings of species, increasing the understanding of mechanisms that rule internal succession behavior
The Inhibition of Phosphoinositide-3 Kinases Induce Resolution of Inflammation in a Gout Model
Phosphoinositide-3 kinases (PI3Ks) are central signaling enzymes that are involved in many aspects of immune cell function. PI3Kγ and PI3Kδ are the major isoforms expressed in leukocytes. The role of PI3K isoforms in the resolution of inflammation is still poorly understood. Here, we investigated the contribution of PI3Kγ and PI3Kδ to the resolution of inflammation in a model of gout in mice.Methods and Results: Experiments were performed in wild-type male C57/Bl6 mice. Selective inhibitors of PI3K-γ (AS605240) or PI3Kδ (GSK045) were injected in the joint 12 h after injection of MSU crystals, hence at the peak of inflammation. Inhibition of either PI3K isoform decreased number of neutrophils that migrated in response to the injection of MSU crystals. This was associated with reduction of myeloperoxidase activity and IL-1β levels in periarticular tissues and reduction of histological score. Joint dysfunction, as seen by reduced mechanical hypernociception, was improved by treatment with either inhibitor. The decrease in neutrophil numbers was associated with enhanced apoptosis and efferocytosis of these cells. There was shortening of resolution intervals, suggesting inhibition of either isoform induced the resolution of neutrophilic inflammation. Blockade of PI3Kγ or PI3Kδ reduced Nuclear Factor kappa B (NF-κB) activation. A pan-PI3K inhibitor (CL27c) reduced inflammation induced by MSU crystals by a magnitude that was similar to that attained by the PI3Kγ or PI3Kδ selective inhibitors alone.Conclusion: Taken together, these results suggest that neutrophils can use PI3Kγ or PI3Kδ to remain in the cavity and blockade of either isoenzyme is sufficient to induce their apoptosis and resolve inflammation in a murine model of gout
iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
A Controversial Role for IL-12 in Immune Response and Bone Resorption at Apical Periodontal Sites
Periapical lesions are inflammatory conditions of tooth periapical tissues, triggered by dental pulp infection and characterized by exudation of immune cells to the affected tissues and production of inflammatory mediators such as cytokines. The inflammatory periapical reaction is mainly driven by Th1, Th2, and Th17 responses, and such polarization may modulate progression of the disease and expression of bone proresorptive cytokines. IL-12 is a potent inducer of IFN-γ production, which stimulates Th1 effector cells. Many evidences have shown a positive correlation between the bone resorptive cytokine IL-1β and the production of IL-12 and IFN-γ. Furthermore, IL-12 may have a potential role in the release of bone resorptive mediators and blockade of Th2 cytokines, affecting the progression of periapical bone loss. Nevertheless, IL-12 and IFN-γ have also been described as suppressors of osteoclast differentiation and activation, favoring bone maintenance. This paper focuses on the controversial roles of IL-12 in periapical lesions
Risk Factors for the Incidence of Dental Caries in Low, Very Low, and Extremely Low Birth Weight Children: A Cohort Study
Objective: To assess the incidence of caries in a two-year period among low birth weight (LBW), very low birth weight (VLBW), and extremely low birth weight (ELBW) children considering socioeconomic indicators, dietary factors and oral hygiene. Material and Methods: A convenience sample was formed of 42 low birth weight children aged two to five years at baseline. Two examiners diagnosed caries using the World Health Organization criteria. Birth weight, socioeconomic indicators and diet were collected from medical records and questionnaires. Binomial models were used to estimate unadjusted and adjusted rate ratios (RR) and respective 95% confidence intervals for the factors evaluated. Results: Thirty-six children were re-examined after two years. The incidence of dental caries was 36.7%. The dmft index was 0.44 (±1.25) at baseline and increased to 1.36 (±3.85) at follow-up. VLBW children (1,000 to 1,500 g) (RR=0.23; 95%CI: 0.08-0.72) and LBW children (1,500 to 2,500 g) (RR=0.06; 0.01-0.55) had fewer carious lesions compared to ELBW children (<1,000 g). Carious lesions were more frequent among children with a lower income (RR=6.05; 1.05-34.84) and less frequent among those who did not consume sweetened juice, tea or yogurt (RR: 0.21; 0.07-0.62). Conclusion:
An inverse dose-response relation was found between birth weight and the incidence of caries. A lower income and the consumption of sweetened beverages were risk factors for the development of caries
The atypical chemokine receptor ACKR2 is protective against sepsis
Sepsis is a systemic inflammatory response as a result of uncontrolled infections. Neutrophils are the first cells to reach the primary sites of infection and chemokines play a key role in recruiting neutrophils. However, in sepsis chemokines could also contribute to neutrophil infiltration to vital organs leading to multiple organ failure. ACKR2 is an atypical chemokine receptor, which can remove and degrade inflammatory CC chemokines. The role of ACK2 in sepsis is unknown. Using a model of cecal ligation and puncture (CLP), we demonstrate here that ACKR2 deficient (−/−) mice exhibited a significant reduction in the survival rate compared to similarly treated wild type (WT) mice. However, neutrophil migration to the peritoneal cavity and bacterial load were similar between WT and ACKR2−/− mice during CLP. In contrast, ACKR2−/− mice showed increased neutrophil infiltration and elevated CC chemokine levels in the lung, kidney and heart compared to the WT mice. In addition, ACKR2−/− mice also showed more severe lesions in the lung and kidney than those in the WT mice. Consistent with these results, WT mice under non-severe sepsis (90% survival) had higher expression of ACKR2 in these organs than mice under severe sepsis (no survival). Finally, the lungs from septic patients showed increased number of ACKR2+ cells compared to those of non-septic patients. Our data indicate that ACKR2 may have a protective role during sepsis, and the absence of ACKR2 leads to exacerbated chemokine accumulation, neutrophil infiltration and damage to vital organs
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