133 research outputs found
X-metabolomics : a software tool for dynamic mass spectroscopy automated signal processing
One of the major challenges of today's biotechnology is
to be able to obtain the maximum of metabolic
information for the holistic interpretation of biological
systems. Herein we present a new computational
application for gas-chromatography mass spectroscopy
automated signal processing, named 'X-Metabolomics',
that shows to be a potential framework for dynamic
systems as fermentation processes interpretation by
compounds and pathways identification and
quantification and moreover for new metabolites
discovery.
Our GC-MS signal processing pipeline is implemented
into an X-window interface using Tcl/Tk interface and
based on R statistical programming environment for
comprehensive statistical computing of results and
access to 'Bioconductor' bioinformatics platform under
Unix, Linux and MacOS.
This approach focuses on the robustness of peak
extraction algorithms for further identification,
quantification and biological interpretation by
multivariate analysis, evolving the following steps: i)
peaks extraction; ii) supervised filtering; iii)
identification of candidate fragments and removal of
possible contaminants; iv) compounds
identification/quantification; v) compounds expression
and co-expression in time-course; and vi) sample
classification and biological interpretation by
multivariate analysis.
'X-Metabolomics' can be an useful tool in different
fields such as pharmacology, genetics, living cells
systems, promising to be innovative and very helpful
for new drug discovery and new advances in dynamic
systems understanding
Inheritance of resistance to Puccinia psidii G. Winter in a eucalyptus interspecific hybrid progeny evaluated under conditions of natural infection
A ferrugem, causada pelo fungo Puccinia psidii, é atualmente a mais importante doença do eucalipto. Esta doença está amplamente disseminada pelo Brasil e causa sérios danos em viveiros e plantações. A identificação de germoplasma resistente aliado ao conhecimento da base genética da resistência são os requerimentos primordiais para o sucesso de programas de melhoramento visando à produção de cultivares resistentes. Estudos anteriores sobre a herança da resistência em condições controladas sugerem tanto uma herança monogênica como a participação de pelo menos 2 genes conferindo resistência à doença. O objetivo deste estudo foi avaliar a resistência a P. psidii, em condições de campo em quatorze progênies obtidas a partir de cruzamentos e auto-cruzamentos controlados entre quatro clones híbridos de Eucalyptus grandis Hill ex Maiden x Eucalyptus urophylla ST Blake,que contrastam para a resistência ao fungo. Os resultados indicam que a resistência pode ser explicada por um loco com efeito principal e pelo menos três alelos diferentes, com interações entre os alelos, resultando no fenótipo de resistência ou suscetibilidade. Entretanto, locos com menor efeito devem influenciar a resistência, uma vez que foram observadas variações nas classes de severidade da escala utilizada. Nenhuma diferença na segregação para a resistência foi observada entre cruzamentos recíprocos, sugerindo que não existe influência citoplasmática no controle deste caráter.Rust caused by the fungus Puccinia psidii is currently the most important disease of eucalyptus. It is widely disseminated in Brazil, and causes serious damage in nurseries and plantation areas. The identification of resistant germplasm along with knowledge of the genetic basis of resistance heredity are the first requirements for the success of breeding programs aiming to develop resistant varieties. Earlier studies carried out under controlled conditions suggested a monogenic control as well as the participation of at least two genes promoting resistance to rust. The goal of this study was to evaluate the resistance to P. psidii under field conditions in fourteen progenies from controlled crosses and self-crosses among four hybrid clones of Eucalyptus grandis Hill ex Maiden x Eucalyptus urophylla ST Blake that contrast for resistance to the fungus. Results indicated that resistance could be explained by one locus with main effects and at least three different alleles. However, loci with minor effects may influence the resistance, since variation on severity classes was observed. Differences in segregation of resistance between reciprocal crosses were not observed, indicating absence of cytoplasmic effects.Votorantim Celulose e PapelConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Long-lived NIR emission in sulfur-doped zeolites due to the presence of [S3]2- clusters
Funding Information: The authors want to acknowledge the Portuguese Foundation for Science and Technology for funding through the grants SFRH/BD/145009/2019 and SFRH/BPD/120599/2016 , the individual contract 2020.00252. CEECIND and several projects PTDC/QUI-QFI/32007/2017, UIDB/04565/2020, UIDP/04565/2020, LA/P/0140/2020, LAQV-REQUIMTE (LA/P/0008/2020, UIDB/50006/2020 and UIDP/50006/2020), VICARTE (UIDP/00729/2020, UIDB/00729/2020) and C2TN (UIDB/04349/2020). MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020) and by European Union's Horizon 2020 research and innovation program under grant agreement No. 810856. Funding Information: The authors want to acknowledge the Portuguese Foundation for Science and Technology for funding through the grants SFRH/BD/145009/2019 and SFRH/BPD/120599/2016, the individual contract 2020.00252. CEECIND and several projects PTDC/QUI-QFI/32007/2017, UIDB/04565/2020, UIDP/04565/2020, LA/P/0140/2020, LAQV-REQUIMTE (LA/P/0008/2020, UIDB/50006/2020 and UIDP/50006/2020), VICARTE (UIDP/00729/2020, UIDB/00729/2020) and C2TN (UIDB/04349/2020). MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020) and by European Union's Horizon 2020 research and innovation program under grant agreement No. 810856. Publisher Copyright: © 2023 The Author(s)The exploration of novel long-lived near-infrared (NIR) luminescent materials has attracted significant attention due to their applications in optical communications, anticounterfeiting, and bioimaging. However, these materials usually present low photoluminescence quantum yields and low photo- and chemical stability. Novel emitters that overcome these limitations are in demand. In this study, NIR emission was achieved using widely available, sustainable, and non-toxic materials through the synthesis of sulfur-doped zeolites, with different S/Cl ratios. With a combination of computational calculations (TD-DFT) and spectroscopic data, this emission was assigned to the radiative decay of excited triplet states of [S3]2- clusters, which resulted in a remarkably high Stokes shift (1.97 eV, 440 nm) and an average decay time of 0.54 ms. These new materials present high stability, external quantum efficiency of up to 17%, and a long-lived NIR emission, placing these compounds in a unique position to be used in applications demanding NIR emitters.publishersversionpublishe
Development of technologies to support the diagnosis of infectious diseases and cancer to support the primary health care
54/2017).
Publisher Copyright:
© 2022, The Author(s).Purpose: Primary Health Care (PHC) is the coordinator of health care in Brazil and needs to be strengthened in the diagnostic field to increase health care quality. Aiming to improve the diagnostic tools currently available in PHC, this work describes the process of development and validation of two point-of-care biomedical devices for screening patients with syphilis or different kinds of cancer. Methods: The development of these devices followed nine stages of action based on the requirements established by the Ministry of Health. During development, both systems followed the stages of circuit planning, software simulation to verify the components used, cost assessment for the acquisition of features, simulation in contact matrix, development of the embedded system, and planning of the printed circuit board and storage box. Results: Both devices underwent preliminary functionality tests to assess their quality. The performance tests applied on the device to diagnose syphilis performed 8,733,194 requests, with a flow of 2426 requests/second, reaching the desired parameters of robustness, integrity, durability, and stability. In addition, functioning tests on the cancer-screening device indicated the ability to detect standard fluorescence in a minimal (150 uL) sample volume. Conclusions: Together, the methodology used for developing the devices resulted in promising equipment to improve the diagnosis and meet the requirements for executing technologies for testing and triaging patients in PHC.publishersversionpublishe
Lower production of IL-17A and increased susceptibility to Mycobacterium bovis in mice coinfected with Strongyloides venezuelensis
The presence of intestinal helminths can down-regulate the immune response required to control mycobacterial infection. BALB/c mice infected with Mycobacterium bovis following an infection with the intestinal helminth Strongyloides venezuelensis showed reduced interleukin-17A production by lung cells and increased bacterial burden. Also, small granulomas and a high accumulation of cells expressing the inhibitory molecule CTLA-4 were observed in the lung. These data suggest that intestinal helminth infection could have a detrimental effect on the control of tuberculosis (TB) and render coinfected individuals more susceptible to the development of TB
Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis
54/2017).
Publisher Copyright:
© 2022 by the authors.The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0′) or 15 min (T15′) after incubation, indicating its potential use as a point-of-care method as a screening strategy.publishersversionpublishe
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