Effective population size in relation to genetic modification of Anopheles gambiae sensu stricto

Effective population size (Ne) is a single number which allows us to relate a large part of population-genetics theory built around ideal populations to real populations. In the case of Anopheles gambiae, Ne is important to interpreting the temporal and spatial distribution of genes. These patterns are in turn used to explore the structure of (typically non-ideal) natural populations. We discuss the complex structure of An. gambiae s.s. in and around Banambani, Mali, as it is currently understood, based on estimates of Ne. This reveals a population that is structured temporally between and within years, spatially between villages and non-dimensionally into chromosomal forms. We suggest that the subpopulations of this species might usefully be viewed as a metapopulation. Successful and efficient genetic modification of An. gambiae will require as complete an understanding of their population structure as possible, which we believe can be attained through the convergence of multiple population-genetic techniques and the application of new method

A method to assess demand growth vulnerability of travel times on road network links

Many national governments around the world have turned their recent focus to monitoring the actual reliability of their road networks. In parallel there have been major research efforts aimed at developing modelling approaches for predicting the potential vulnerability of such networks, and in forecasting the future impact of any mitigating actions. In practice-whether monitoring the past or planning for the future-a confounding factor may arise, namely the potential for systematic growth in demand over a period of years. As this growth occurs the networks will operate in a regime closer to capacity, in which they are more sensitive to any variation in flow or capacity. Such growth will be partially an explanation for trends observed in historic data, and it will have an impact in forecasting too, where we can interpret this as implying that the networks are vulnerable to demand growth. This fact is not reflected in current vulnerability methods which focus almost exclusively on vulnerability to loss in capacity. In the paper, a simple, moment-based method is developed to separate out this effect of demand growth on the distribution of travel times on a network link, the aim being to develop a simple, tractable, analytic method for medium-term planning applications. Thus the impact of demand growth on the mean, variance and skewness in travel times may be isolated. For given critical changes in these summary measures, we are thus able to identify what (location-specific) level of demand growth would cause these critical values to be exceeded, and this level is referred to as Demand Growth Reliability Vulnerability (DGRV). Computing the DGRV index for each link of a network also allows the planner to identify the most vulnerable locations, in terms of their ability to accommodate growth in demand. Numerical examples are used to illustrate the principles and computation of the DGRV measure

Chromatin accessibility reveals insights into androgen receptor activation and transcriptional specificity

BACKGROUND: Epigenetic mechanisms such as chromatin accessibility impact transcription factor binding to DNA and transcriptional specificity. The androgen receptor (AR), a master regulator of the male phenotype and prostate cancer pathogenesis, acts primarily through ligand-activated transcription of target genes. Although several determinants of AR transcriptional specificity have been elucidated, our understanding of the interplay between chromatin accessibility and AR function remains incomplete. RESULTS: We used deep sequencing to assess chromatin structure via DNase I hypersensitivity and mRNA abundance, and paired these datasets with three independent AR ChIP-seq datasets. Our analysis revealed qualitative and quantitative differences in chromatin accessibility that corresponded to both AR binding and an enrichment of motifs for potential collaborating factors, one of which was identified as SP1. These quantitative differences were significantly associated with AR-regulated mRNA transcription across the genome. Base-pair resolution of the DNase I cleavage profile revealed three distinct footprinting patterns associated with the AR-DNA interaction, suggesting multiple modes of AR interaction with the genome. CONCLUSIONS: In contrast with other DNA-binding factors, AR binding to the genome does not only target regions that are accessible to DNase I cleavage prior to hormone induction. AR binding is invariably associated with an increase in chromatin accessibility and, consequently, changes in gene expression. Furthermore, we present the first in vivo evidence that a significant fraction of AR binds only to half of the full AR DNA motif. These findings indicate a dynamic quantitative relationship between chromatin structure and AR-DNA binding that impacts AR transcriptional specificity

Diffusion in supersonic, turbulent, compressible flows

We investigate diffusion in supersonic, turbulent, compressible flows. Supersonic turbulence can be characterized as network of interacting shocks. We consider flows with different rms Mach numbers and where energy necessary to maintain dynamical equilibrium is inserted at different spatial scales. We find that turbulent transport exhibits super-diffusive behavior due to induced bulk motions. In a comoving reference frame, however, diffusion behaves normal and can be described by mixing length theory extended into the supersonic regime.Comment: 11 pages, incl. 5 figures, accepted for publication in Physical Review E (a high-resolution version is available at http://www.aip.de./~ralf/Publications/p21.abstract.html

Soil biochemistry and microbial activity in vineyards under conventional and organic management at Northeast Brazil.

The São Francisco Submedium Valley is located at the Brazilian semiarid region and is an important center for irrigated fruit growing. This region is responsible for 97% of the national exportation of table grapes, including seedless grapes. Based on the fact that orgThe São Francisco Submedium Valley is located at the Brazilian semiarid region and is an important center for irrigated fruit growing. This region is responsible for 97% of the national exportation of table grapes, including seedless grapes. Based on the fact that organic fertilization can improve soil quality, we compared the effects of conventional and organic soil management on microbial activity and mycorrhization of seedless grape crops. We measured glomerospores number, most probable number (MPN) of propagules, richness of arbuscular mycorrhizal fungi (AMF) species, AMF root colonization, EE-BRSP production, carbon microbial biomass (C-MB), microbial respiration, fluorescein diacetate hydrolytic activity (FDA) and metabolic coefficient (qCO2). The organic management led to an increase in all variables with the exception of EE-BRSP and qCO2. Mycorrhizal colonization increased from 4.7% in conventional crops to 15.9% in organic crops. Spore number ranged from 4.1 to 12.4 per 50 g-1 soil in both management systems. The most probable number of AMF propagules increased from 79 cm-3 soil in the conventional system to 110 cm-3 soil in the organic system. Microbial carbon, CO2 emission, and FDA activity were increased by 100 to 200% in the organic crop. Thirteen species of AMF were identified, the majority in the organic cultivation system. Acaulospora excavata, Entrophospora infrequens, Glomus sp.3 and Scutellospora sp. were found only in the organically managed crop. S. gregaria was found only in the conventional crop. Organically managed vineyards increased mycorrhization and general soil microbial activity

Seagrass spatial data synthesis from north-east Australia, Torres Strait and Gulf of Carpentaria, 1983 to 2022

The Gulf of Carpentaria and Torres Strait in north-eastern Australia support globally significant seagrass ecosystems that underpin fishing and cultural heritage of the region. Reliable data on seagrass distribution are critical to understanding how these ecosystems are changing, while managing for resilience. Spatial data on seagrass have been collected since the early 1980s, but the early data were poorly curated. Some was not publicly available, and some already lost. We validated and synthesized historical seagrass spatial data to create a publicly available database. We include a site layer of 48,612 geolocated data points including information on seagrass presence/absence, sediment, collection date, and data custodian. We include a polygon layer with 641 individual seagrass meadows. Thirteen seagrass species are identified in depths ranging from intertidal to 38 m below mean sea level. Our synthesis includes scientific survey data from 1983 to 2022 and provides an important evidence base for marine resource management

Effect of mineralocorticoid receptor antagonists on proteinuria and progression of chronic kidney disease: A systematic review and meta-analysis

Background: Hypertension and proteinuria are critically involved in the progression of chronic kidney disease. Despite treatment with renin angiotensin system inhibition, kidney function declines in many patients. Aldosterone excess is a risk factor for progression of kidney disease. Hyperkalaemia is a concern with the use of mineralocorticoid receptor antagonists. We aimed to determine whether the renal protective benefits of mineralocorticoid antagonists outweigh the risk of hyperkalaemia associated with this treatment in patients with chronic kidney disease. Methods: We conducted a meta-analysis investigating renoprotective effects and risk of hyperkalaemia in trials of mineralocorticoid receptor antagonists in chronic kidney disease. Trials were identified from MEDLINE (1966-2014), EMBASE (1947-2014) and the Cochrane Clinical Trials Database. Unpublished summary data were obtained from investigators. We included randomised controlled trials, and the first period of randomised cross over trials lasting ≥4 weeks in adults. Results: Nineteen trials (21 study groups, 1 646 patients) were included. In random effects meta-analysis, addition of mineralocorticoid receptor antagonists to renin angiotensin system inhibition resulted in a reduction from baseline in systolic blood pressure (-5.7 [-9.0, -2.3] mmHg), diastolic blood pressure (-1.7 [-3.4, -0.1] mmHg) and glomerular filtration rate (-3.2 [-5.4, -1.0] mL/min/1.73 m2). Mineralocorticoid receptor antagonism reduced weighted mean protein/albumin excretion by 38.7 % but with a threefold higher relative risk of withdrawing from the trial due to hyperkalaemia (3.21, [1.19, 8.71]). Death, cardiovascular events and hard renal end points were not reported in sufficient numbers to analyse. Conclusions: Mineralocorticoid receptor antagonism reduces blood pressure and urinary protein/albumin excretion with a quantifiable risk of hyperkalaemia above predefined study upper limit

Observation of Ds1(2536)+ -> D+pi-K+ and angular decomposition of Ds1(2536)+ -> D*+K0S

Using 462/fb of e+e- annihilation data recorded by the Belle detector, we report the first observation of the decay Ds1(2536)+ -> D+pi-K+. The ratio of branching fractions B(Ds1+ -> D+pi-K+)/B(Ds1+ -> D*+K0) is measured to be (3.27+-0.18+-0.37)%. We also study the angular distributions in the Ds1(2536)+ -> D*+K0S decay and measure the ratio of D- and S-wave amplitudes. The S-wave dominates, with a partial width of Gamma_S/Gamma_total=0.72+-0.05+-0.01.Comment: Submitted to Phys.Rev.D 16 pages, 6 figures, 3 table