The Role of the Circadian Clock in Fat Body Transcriptomics and Metabolomics

Drosophila melanogaster circadian rhythms oscillate based on many different factors and are dictated by the central brain clock, input pathways that take in outside information, and output pathways. These output pathways include peripheral tissues that can affect many different behaviors. An example of one of these tissues is the fat body, which may play a role in feeding/fasting rhythms. The mechanism used to control this behavior is not well known. By determining a connection between the central brain clock and peripheral tissues such as the fat body, the way that physiological processes such as metabolism, energy storage, and behavioral outputs are controlled can be better understood. Long term, this project aims to identify connections between metabolic rhythms utilizing metabolomics and transcriptomic of the fat body. To do so, the best, most specific driver with which to manipulate the fat body must be identified, and the expression of any other tissues must be identified. Using the GAL4-UAS system, the manipulation or selective inactivation of fat body tissues can be accomplished by certain drivers. Takeout-GAL4 is well-known as a fat body driver but may show expression in other tissues which must be identified in more detail. Lsp is another driver that must be characterized and compared to Takeout-GAL4. By characterizing each driver in detail, the most selective one can be utilized in later experiments to learn more about circadian relation to metabolism

The Ursinus Weekly, February 7, 1944

Sally Deibler sells $1400 in bonds as fourth loan drive reaches new high • Loughead charms top crowd at festive quarterdeck hop • Typical student is quiet and reserved at Ursinus College • Dr. James Dean gives talk on central nervous system • Cap is aiding war effort, seeks air-minded recruits • French Club will feature songs, games at meeting • Joh Ziegler addresses student body at vespers • Community club plans public forum on labor relations topic, Feb. 8 • Y party to feature ace novelty act • Don\u27t be be a Joe or Maisie Zilch, try studying now for those exams • Phys-edders meet tonight • Perkiomen AAUW to hear speech on four freedoms • Ursinus debaters to meet Kutztown team tomorrow • War prisoners receive thousands of books • Improved girls\u27 team defeats Albright, Rosemont sextettes • Juniata downs bears in close contest, 64-52 • Hauser and Moore set pace as bears take Swarthmore • Lynnewood downs south in first inter-dorm game • Ursinus wrestlers to meet Muhlenberg this Saturday • Jack Bradford has sandwich shop where the elite meet to eat - meathttps://digitalcommons.ursinus.edu/weekly/1724/thumbnail.jp

Protective Factors of Low Bone Mineral Density: An Examination between Two Samples of Premenopausal Women

While minimal longitudinal data exists to support osteoporosis screening among premenopausal women, an increasingly modernized society may contribute to modifiable osteoporosis risk factors including poor nutrition and low physical activity. Evaluation of bone mineral density (BMD) and body composition differences in populations with varied levels of physical activity is of importance. PURPOSE: The purpose of the current research was to determine if there is a difference across body composition measurements including total lean mass, body mass index (BMI), and BMD among a subset of physically active premenopausal women (ACTIVE) at a small, southern Christian university compared to premenopausal women from the general U.S. population in the National Health and Nutrition Examination Survey (NHANES). METHODS: BMD z-scores were calculated for premenopausal females who underwent a Dual-Energy X-Ray Absorptiometry (DXA) scan in a university performance lab from 2009 to 2023, matched to age, sex, and race/ethnicity participants from NHANES. Participants were classified by low or normal BMD using the International Society for Clinical Densitometry recommended cutoff of -2.0 for total body and by BMI using standard weight status categories published by the Centers for Disease Control and Prevention. Descriptive statistics were used to analyze participant characteristics and percentage across BMI and BMD categories. Total lean mass was reported by mean and standard deviation for ACTIVE and NHANES participants across BMI and BMD categories. Independent t-tests were used to determine if any differences existed between the ACTIVE and NHANES participants across total lean mass, BMI, and BMD. RESULTS: Data analysis included 2037 from the ACTIVE sample and 4326 from the NHANES sample. The frequency of low BMD (\u3c-2.0) among ACTIVE participants is 0.39% (n = 8) compared to 3.7% (n = 78) among NHANES participants. ACTIVE participants had significantly higher BMD [t(3361.21) = 26.49, p \u3c 0.001] and a significantly greater total lean mass [t(4677.71) = 11.14, p \u3c 0.01) than NHANES participants. Intriguingly, ACTIVE participants had a higher mean total lean mass than NHANES participants across all BMI categories except those classified as obese, regardless of BMD. CONCLUSION: The ACTIVE sample demonstrated a decreased prevalence of low BMD, likely attributed to increased physical activity. These observed differences in BMD are supported by higher total lean mass and lower rates of obesity likely also attributed to their physical activity history. This data supports the importance of lifestyle habits and its beneficial effects on both BMD and indices of body composition among premenopausal women

Columbus State University Honors College: Senior Theses, Fall 2019

This is a collection of senior theses written by honors students at Columbus State University in 2019.https://csuepress.columbusstate.edu/honors_theses/1000/thumbnail.jp

A sensitive high performance liquid chromatography assay for the quantification of doxorubicin associated with DNA in tumor and tissues

A HPLC method was validated to quantify doxorubicin associated to DNA from tissue.Successfully applied to an in vivo mouse-based pharmacokinetic study.Important tool for future studies evaluating intracellular pharmacokinetics.Doxorubicin, a widely used anticancer agent, exhibits antitumor activity against a wide variety of malignancies. The drug exerts its cytotoxic effects by binding to and intercalating within the DNA of tumor and tissue cells. However, current assays are unable to accurately determine the concentration of the intracellular active form of doxorubicin. Thus, the development of a sample processing method and a high-performance liquid chromatography (HPLC) methodology was performed in order to quantify doxorubicin that is associated with DNA in tumors and tissues, which provided an intracellular cytotoxic measure of doxorubicin exposure after administration of small molecule and nanoparticle formulations of doxorubicin. The assay uses daunorubicin as an internal standard; liquid–liquid phase extraction to isolate drug associated with DNA; a Shimadzu HPLC with fluorescence detection equipped with a Phenomenex Luna C18 (2 μm, 2.0 × 100 mm) analytical column and a gradient mobile phase of 0.1% formic acid in water or acetonitrile for separation and quantification. The assay has a lower limit of detection (LLOQ) of 10 ng/mL and is shown to be linear up to 3000 ng/mL. The intra- and inter-day precision of the assay expressed as a coefficient of variation (CV%) ranged from 4.01 to 8.81%. Furthermore, the suitability of this assay for measuring doxorubicin associated with DNA in vivo was demonstrated by using it to quantify the doxorubicin concentration within tumor samples from SKOV3 and HEC1A mice obtained 72 h after administration of PEGylated liposomal doxorubicin (Doxil®; PLD) at 6 mg/kg IV x 1. This HPLC assay allows for sensitive intracellular quantification of doxorubicin and will be an important tool for future studies evaluating intracellular pharmacokinetics of doxorubicin and various nanoparticle formulations of doxorubicin

The Ursinus Weekly, January 10, 1944

Fine cast chosen for coming play, Jupiter Laughs • Ensign Miriam Waltemyer to speak on Navy waves • Senator Ball will speak at Ursinus this Wednesday on post-war world • Ursinus honor grad to speak tonight • Dorothy Waltz engaged • Intersorority dance postponed • Students joyful at belated banquet • Rosicrucians elect girls to fill coveted offices • Memorial marks site of girls\u27 seminary • Post-war employment ideas solicited in Pabst contest • Dr. Hartzell holds office on Collegeville council • Combined Y\u27s will sponsor amateur night on Friday • Rev. Shaffer addresses student body at vespers • Ursinus students speak • Publishers offer awards to writers in services • German Club features sing • James Boswell to teach mathematics at Illinois • Leona Miller to give make-up demonstration • Captain Fury presented • Loraine Walton to review Taps for Private Tussie • The librarian\u27s angle • Courtmen lose close tilt to F. & M. when Mackin scores in last minute • Garnet crushes Ursinus grapplers • Carney beats Shope in intramural games • Men\u27s varsity defeats Valley Forge hospital • Five girls return from 1943 varsity • Freshman receives rating in 1943 tennis lineup • Basketball five downs Superior Tube team • War cannot stop Russian colleges • Ursinus students flock to Thompson-Gay gymhttps://digitalcommons.ursinus.edu/weekly/1722/thumbnail.jp

N- to C-sulfonyl photoisomerisation of dihydropyridinones : a synthetic and mechanistic study

The authors thank the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007–2013) ERC Grant Agreement no. 279850 (CF and JET) and EPSRC grant number EP/J018139/1 (DSBD) for funding. ADS thanks the Royal Society for a Wolfson Research Merit Award.The scope and limitations of a photoinitiated N- to C-sulfonyl migration process within a range of dihydropyridinones is assessed. This sulfonyl transfer proceeds without erosion of either diastereo- or enantiocontrol, and is general across a range of N-sulfonyl substituents (SO2R; R = Ph, 4-MeC6H4, 4-MeOC6H4, 4-NO2C6H4, Me, Et) as well as C(3)-(aryl, heteroaryl, alkyl and alkenyl) and C(4)-(aryl and ester) substitution. Crossover reactions indicate an intermolecular step is operative within the formal migration process, although no crossover from C-sulfonyl products was observed. EPR studies indicate the intermediacy of a sulfonyl radical and a mechanism is proposed based upon these observations.Publisher PDFPeer reviewe

Is it safe to move away from a full sternotomy for aortic valve replacement?

Minimally invasive surgical approaches have gained popularity among patients and surgeons. The aim of this project was to assess the safety of initiating aortic valve replacement via an anterior right thoracotomy program

The Lantern Vol. 14, No. 2, February 1946

• Dog Daze • Locomotion • The Battle • Thoughts at Midnight • We Have a Race to Run • A Parable • Darkness at Dawn • Room for Error • Elegy Americana • Will This Happen Here • Last Mission • Free Trade • Love Letterhttps://digitalcommons.ursinus.edu/lantern/1038/thumbnail.jp