United Methodist Children\u27s Center Playground Improvements

The project consists of multiple pieces that were done as a team to improve the outdoor areas of the United Methodist Children’s Center. The scope of the work consists of placing gorilla hair mulch, making a bench for the kids, removing, grading, and placing artificial turf, and replacing the canvas of a shade structure. There are a few other smaller pieces that will be completed as well but those are the main pieces of the project. The project was executed by teaming up with multiple companies and getting teams of students together to construct the pieces of the project in the timeline we were given. Being a children’s center, there was a challenge with working around their schedule and not interfering with the kids. This project began in February of 2023 and was completed on June 4, 2023. The children’s center was left with a cleaner, safer playground for their students to enjoy during their recess, and the members of the project team left with a new understanding of what goes into placing turf, mulch, building benches, and replacing a canvas shade structure

Metaanalyse zur genetischen Prädisposition bei Migräne unter Verwendung von Polymorphismen

Die Migräne gehört zu den primären Kopfschmerzarten und gilt als eine der häufigsten Ursachen für Kopfschmerzen. Es scheinen mehrere Faktoren eine Rolle bei der Pathogenese zu spielen, darunter auch genetische, wofür das familiäre Auftreten spricht1. Polymorphismen auf Genabschnitten für Östrogenhormonrezeptoren, Neurotransmitter-rezeptoren, für am Katecholaminstoffwechsel und am Folatstoffwechsel beteiligte Enzyme sowie für Zytokine wurden in verschiedenen Studien auf ihre Auswirkung hinsichtlich der Pathophysiologie der Migräne anhand von Fall-Kontroll-Gruppen untersucht und kamen zum Teil auf unterschiedliche Ergebnisse. Im Rahmen dieser Dissertation wurde daher eine Metaanalyse durchgeführt, die die Studien zu den Polymorphismen ESR1 rs2228480, ESR1 rs1801132, ESR1 rs2234693, GRIA1 rs548294, 5-HT1A rs6295, DBH rs1611115, COMT rs4680, MTHFR rs1801133 und TNF alpha 1800629 zusammenfasst. Es wurde die Literaturdatenbank PubMed für die Suche der einzelnen Studien verwendet. Anschließend wurde sowohl für das dominante als auch für das rezessive Modell die Odds Ratio und das 95%-Konfidenzintervall berechnet. Es wurden insgesamt 68 Fall-Kontroll-Gruppen mit 21558 Migränepatienten und 40849 Kontrollpersonen in die Metaanalyse eingeschlossen. Die Ergebnisse der Analyse zum Polymorphismus ESR1 rs2228480 lassen vermuten, dass Personen mit dem Genotyp AA ein höheres Risiko haben, an Migräne zu erkranken. Auch das Ergebnis der Analyse zum Polymorphismus ESR1 rs1801132 weist darauf hin, dass Personen mit dem Genotyp GG ein erhöhtes Risiko tragen, eine Migräne zu entwickeln. Bei den anderen sieben Polymorphismen war kein statistisch signifikanter Zusammenhang hinsichtlich des Polymorphismus und des Erkrankungsrisikos für Migräne ersichtlich.Migraine is one of the primary headaches and is one of the most common causes of headaches. Several factors appear to play a role in pathogenesis of migraine, including genetic, as the familial occurrence shows1. Polymorphisms on gene sections for estrogen hormone receptors, neurotransmitter receptors, enzymes involved in katecholamine metabolism and folate metabolism as well as for cytokines were examined in various studies for their effect on the pathophysiology of migraine by case-control groups. In this thesis, a meta-analysis was performed that summarizes the studies on the polymorphisms ESR1 rs2228480, ESR1 rs1801132, ESR1 rs2234693, GRIA1 rs548294, 5-HT1A rs6295, DBH rs1611115, COMT rs4680, MTHFR rs1801133 and TNF alpha 1800629. The literature database PubMed was used for the search of the individual studies. The odds ratio and the 95% confidence interval were then calculated for both, the dominant and the recessive model. A total of 68 case control groups with 21558 migraine patients and 40849 controls were included in the meta-analysis. The results of the analysis on the polymorphism ESR1 rs2228480 suggest that persons with the genotype AA have a higher risk of developing migraine. Also, the result of the analysis on the polymorphism ESR1 rs1801132 indicates that individuals with the genotype GG carry an increased risk of developing migraine. In the other seven polymorphisms, no statistically significant association was observed with regard to the polymorphism and the risk of migraine

Bacchic Echoes: Dionysus as Metaphor in Apollonius' Argonautica

This thesis argues that an interpretation of Apollonius’ Argonautica must take account of a Dionysiac schema mobilising the epic’s meanings: this schema operates as an organising principle for understanding the epic’s initial reception not only because Hellenistic literary production is steeped in Dionysiac forms that gave prominence to Attic tragedy and theatrical performance, but also because Dionysiac cult played a critical role in Ptolemaic royal ideology both ritually and politically. As Hellenistic epic proclaimed and bolstered Ptolemaic regional and political hegemonic claims, the Argonautica ought to be expected to contain a reflex of this Ptolemaic focus on Dionysus. (1) Euripides’ Bacchae provides the main frame and a patterning (the ‘hospitality plot’) within which Argonautic action can be invested with Bacchic significance: resistance to foreign strangers, inversions of identity and power, Dionysiac social cohesion, intimacy with natural environments, the Argonauts as a chorus with Jason as 'exarchos' and Orpheus as 'choregos'/'koryphaios', etc. Bacchic intertextuality animates meaning in the Argonautica. (2) The women of the Argonautica are demonstrably Maenadic. Jason’s relationship with Medea is mapped onto the model of the ‘tragic marriage’ which opposes the endogamy of über-elite households with a civic cohesion resulting from the mixing of citizen households. This triggers a destructive necessity to admit the foreigner into the 'oikos'/'polis' which, as R. Seaford argues, was so allergic to tyrants and royal houses in tragedy and, of these, the Bacchae most programmatically. The maenadic submission of local women to Dionysus in tragedy offers a way of reframing the meanings of Medea’s seduction by and escape with Jason. (3) Jason is assimilated figurally to the god Dionysus and emerges as an epic hero caught between twin pairs of Dionysian imperative: the literary and the cultural-political on the one hand, and between the Euripidean martial Dionysus and the ‘Philadelphian’ cosmopolitan wine-god on the other. In the end, Apollonius’ innovation in the Argonautica is the elaboration of a Dionysian epic hero apt for the third century Alexandrian tastes of the court of Philadelphus

Synthetic Communities of Bacterial Endophytes to Improve the Quality and Yield of Legume Crops

Plant-associated microbiomes confer fitness advantages to the plant host by growth promotion through different mechanisms including nutrient uptake, phytohormones production, resistance to pathogens, and stress tolerance. These effects of the potentially beneficial microbes have been used in a diversity of biotechnological approaches to improve crop performance applying individual bacterial cultures. However, healthy plants host a diversity of microorganisms (microbiota). Next-generation sequencing technologies have offered insights into the relative abundances of different phylogenetic groups in a community and the metabolic and physiological potential of its members. In the last decade, researchers have started to explore the possibilities to use temporal and functional combinations of those bacteria in the form of synthetic communities. In this chapter, we review the benefits of using endophytic bacteria in legumes, the available methodological approaches to study the effects of bacterial communities, and the most recent findings using synthetic communities to improve the performance of legume crops.Instituto de Patología VegetalFil: Monteoliva, Mariela Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Monteoliva, Mariela Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Taurian, Tania. Universidad Nacional de Río Cuarto (UNRC). Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Guzzo, María Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Guzzo, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentin

Peanut Endophytic Phosphate Solubilizing Bacteria Increase Growth and P Content of Soybean and Maize Plants

Phosphorus (P) is a limiting factor of plant development due to its low availability in the soil. The use of endophytic phosphate solubilizing bacteria as a more sustainable alternative to the use of chemical phosphorus fertilizers is proposed in this study. The objectives were to analyze the effect of simple inoculations of native peanut endophytic phosphate solubilizing bacteria on plant growth promotion and P content of soybean and maize and to evaluate their survival and endophytic colonization capacity on these plants. In addition, bacterial plant cell wall degrading enzymes activities in presence or absence of root exudates was determined. Soybean, maize and peanut plants were grown on a microcosm scale and inoculated with Enterobacter sp. J49 or Serratia sp. S119. It was observed that phosphate solubilizing strains promoted the growth of maize and soybean plants and contributed significantly P to their tissues. A significant increase in the phosphate solubilizing capacity of the plant rhizosphere after the end of the assay was observed. The strains showed to survive in plant’s growth substrate and in the case of Enterobacter sp. J49, it showed also to colonize endophytically maize and soybean. Root exudates of the three plants showed to produce changes in pectinase and cellulase activities of the strains. The bacterial strains analyzed in this study constitutes potential sources for the formulation of biofertilizers for their application for several crops in agricultural soils with low P content.Fil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa; ArgentinaFil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Taurian, Tania. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentin

Motility and biofilm production involved in the interaction of phosphate solubilizing endophytic strains with peanut, maize and soybean plants

Endophytic plant growth promoting bacteria are of great interest since the ability to colonize the internal tissues of plants gives it an adaptive advantage. To confer any beneficial effect on the plant, bacteria must present a successful colonization and thus be competent in the rhizosphere and finally infect internal tissues. In this sense, bacterial motility is an essential property involved in plant-microorganism interaction where bacteria can colonize the root and at the same time form biofilms. Within plant beneficial bacteria those that present phosphate solubilizing activity are of great interest due to the contribution of phosphorus to plants. The objective of this work was to evaluate the motility and biofilm formation properties of the strains Serratia sp. S119 and Enterobacter sp. J49 growing in the presence of peanut, maize and soybean root exudates. Results obtained indicated that both strains have flagella, possess swimming, swarming and twitching motilities and showed differential chemotactic attraction against root exudates. In addition, the strains under study showed the ability to form biofilms, being this ability greater in minimal media, in which a greater decrease was also seen by the addition of root exudates in the first hours of growth. The changes produced by the addition of root exudates in the chemotaxis and biofilm formation of the strains suggests that compounds released by the plants are detected by these bacteria and could be part of the molecular dialogue involved in their interaction with the roots of plant.Fil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Anzuay, María Soledad. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Taurian, Tania. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentin