Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA as Rab-effector kinases in malaria parasites

Background information The pathology causing stages of the human malaria parasite Plasmodium falciparum reside within red blood cells that are devoid of any regulated transport system. The parasite, therefore, is entirely responsible for mediating vesicular transport within itself and in the infected erythrocyte cytoplasm, and it does so in part via its family of 11 Rab GTPases. Putative functions have been ascribed to Plasmodium Rabs due to their homology with Rabs of yeast, particularly with Saccharomyces that has an equivalent number of rab/ypt genes and where analyses of Ypt function is well characterized. Results Rabs are important regulators of vesicular traffic due to their capacity to recruit specific effectors. In order to identify P. falciparum Rab (PfRab) effectors, we first built a Ypt-interactome by exploiting genetic and physical binding data available at the Saccharomyces genome database (SGD). We then constructed a PfRab-interactome using putative parasite Rab-effectors identified by homology to Ypt-effectors. We demonstrate its potential by wet-bench testing three predictions; that casein kinase-1 (PfCK1) is a specific Rab5B interacting protein and that the catalytic subunit of cAMP-dependent protein kinase A (PfPKA-C) is a PfRab5A and PfRab7 effector. Conclusions The establishment of a shared set of physical Ypt/PfRab-effector proteins sheds light on a core set Plasmodium Rab-interactants shared with yeast. The PfRab-interactome should benefit vesicular trafficking studies in malaria parasites. The recruitment of PfCK1 to PfRab5B+ and PfPKA-C to PfRab5A+ and PfRab7+ vesicles, respectively, suggests that PfRab-recruited kinases potentially play a role in early and late endosome function in malaria parasites

Summer School Report: Jyväskylä Summer School Course on Ultrafast Spectroscopy

The 32nd edition of the Jyväskylä Summer School (JSS32) was in high demand for the ultrafast spectroscopy chapter. On August 7–11 2023 nearly 40 students gathered to Jyväskylä to tune in for the JSS32 course entitled UltrafastSpectroscopy: phenomena, experiments and data analysis methods. The course was coordinated by Senior Lecturer Tatu Kumpulainen and featured lectures each morning from two invited speakers, Prof. Eric Vauthey, University of Geneva, Switzerland, and Assoc. Prof. Jens Uhlig, Lund University, Sweden. In addition to the lectures, the course featured four workshops in the afternoons aimed at introducing the participants to the practical aspects of steady-state and time-resolved spectroscopies. Besides the scientific content, JSS32 offered several social events where participants could network with other participants of the whole summer school

Orientation gradients in rapidly solidified pure aluminum thin films: comparison of experiments and phase-field crystal simulations

Rapid solidification experiments on thin film aluminum samples reveal the presence of lattice orientation gradients within crystallizing grains. To study this phenomenon, a single-component phase-field crystal (PFC) model that captures the properties of solid, liquid, and vapor phases is proposed to model pure aluminium quantitatively. A coarse-grained amplitude representation of this model is used to simulate solidification in samples approaching micrometer scales. The simulations reproduce the experimentally observed orientation gradients within crystallizing grains when grown at experimentally relevant rapid quenches. We propose a causal connection between formation of defects and orientation gradients

Treatment of medial-sided injuries in patients with early bicruciate ligament reconstruction for knee dislocation

Purpose In knee dislocation with bicruciate ligament and medial side injury (KDIIIM), treatment method of medial side injuries is controversial. The purpose of this study was to evaluate the outcomes of non-operative treatment of proximal and midsubstance and operative treatment of distal avulsion medial collateral ligament (MCL) ruptures in patients with early bicruciate reconstruction. Methods One-hundred and forty-seven patients with a knee dislocation and bicruciate ligament injury (KDII-KDV) were identified. Sixty-two patients had KDIIIM injury. Of these, 24 patients were excluded and 13 were lost to follow-up. With a minimum of 2 years of follow-up, IKDC2000 (subjective and objective), Lysholm and Tegner scores and stress radiographs were recorded. Results Twenty-five patients were available for follow-up: 18 had a proximal or midsubstance grade-III MCL rupture (proximal MCL group) and 7 had a distal MCL avulsion (distal MCL group). In the proximal MCL and distal MCL groups, respectively, median IKDC2000 subjective scores were 80 (range 57-99) and 62 (range 39-87), and median Lysholm scores were 88 (range 57-99) and 75 (range 40-100). The median medial opening (side-to-side difference) was 2.4 mm (range 0.1-9.2) in the proximal MCL group and 2.5 mm (range 0.2-4.8) in the distal MCL group. Conclusion We found acceptable recorded outcomes in patients who underwent non-operative treatment of proximal and midsubstance grade-III MCL rupture and operative treatment of distal MCL avulsion with early bicruciate ligament reconstruction.Peer reviewe

Temporal and spatial neural dynamics in the perception of basic emotions from complex scenes

The different temporal dynamics of emotions are critical to understand their evolutionary role in the regulation of interactions with the surrounding environment. Here, we investigated the temporal dynamics underlying the perception of four basic emotions from complex scenes varying in valence and arousal (fear, disgust, happiness and sadness) with the millisecond time resolution of Electroencephalography (EEG). Event-related potentials were computed and each emotion showed a specific temporal profile, as revealed by distinct time segments of significant differences from the neutral scenes. Fear perception elicited significant activity at the earliest time segments, followed by disgust, happiness and sadness. Moreover, fear, disgust and happiness were characterized by two time segments of significant activity, whereas sadness showed only one long-latency time segment of activity. Multidimensional scaling was used to assess the correspondence between neural temporal dynamics and the subjective experience elicited by the four emotions in a subsequent behavioral task. We found a high coherence between these two classes of data, indicating that psychological categories defining emotions have a close correspondence at the brain level in terms of neural temporal dynamics. Finally, we localized the brain regions of time-dependent activity for each emotion and time segment with the low-resolution brain electromagnetic tomography. Fear and disgust showed widely distributed activations, predominantly in the right hemisphere. Happiness activated a number of areas mostly in the left hemisphere, whereas sadness showed a limited number of active areas at late latency. The present findings indicate that the neural signature of basic emotions can emerge as the byproduct of dynamic spatiotemporal brain networks as investigated with millisecond-range resolution, rather than in time-independent areas involved uniquely in the processing one specific emotion. Keywords: basic emotions, EEG, LORETA, ERP, IAPS, time, rapid perceptio

PENGARUH SISTEM INFORMASI AKUNTANSI TERHADAP KINERJA KARYAWAN BADAN USAHA MILIK DESA (BUMDesa) DI KABUPATEN MANGGARAI TIMUR

ABSTRACT In the era of government now the village plays an important role where the village is the front guard to identify any problems that exist in society.  One way for the village to prosper the community is to establish a Village-Owned Enterprise (BUMDesa).  This study aims to determine whether there is an influence of the accounting information system on the performance of BUMDesa employees / administrators in East Manggarai Regency.  This research uses a mix method.  This research was conducted at 12 BUMDesa in East Manggarai Regency.  The results of this study indicate that the accounting information system has a positive effect on employee / management performance in East Manggarai Regency.  The magnitude of the influence of the accounting information system on performance is 29.8%.  The results of the interviews also showed the same results where from the elaboration of each indicator the researchers concluded that the accounting information system affected the performance of BUMDesa employees/administrators in East Manggarai Regency.   Keywords: Accounting Information System, Performance of Employees, Village Owned EnterprisesABSTRAK Di era pemerintahan sekarang desa memainkan peranan yang penting dimana desa menjadi garda terdepan untuk mengidentifikasi setiap persoalan yang ada dalam masyarakat. Salah satu cara desa untuk mensejahterakan masyarakat adalah dengan mendirikan Badan Usaha Milik Desa (BUMDesa). Penelitian ini bertujuan untuk mengetahui apakah terdapat pengaruh dari sistem informasi akuntansi terhadap kinerja karyawan/pengurus BUMDesa di Kabupaten Manggarai Timur. Penelitian ini menggunakan mix method. Penelitian ini dilakukan pada 12 BUMDesa yang ada di Kabupaten Manggarai Timur. Hasil penelitian ini menunjukan bahwa sistem informasi akuntansi berpengaruh positif terhadap kinerja karyawan/pengurus  di Kabupaten Manggarai Timur. Besarnya pengaruh dari sistem informasi akuntansi terhadap kinerja adalah sebesar 29,8%. Hasil wawancara juga menunjukan hasil yang sama dimana dari penjabaran setiap indikator peneliti dapat menyimpulkan bahwa sistem informasi akuntansi berpengaruh terhadap kinerja karyawan/pengurus BUMDesa di Kabupaten Manggarai Timur. Katakunci: Sistem Informasi Akuntansi, Kinerja Karyawan, BUMDes

Executive Summary: Building Holistic Security

Women peacebuilders inevitably face risks and insecurity in their daily work. International partners have an important role to play in supporting their safety and protection. Understanding women peacebuilders’ roles and the types of risks they face is the first step in ensuring an adequate response. The diversity of roles that women peacebuilders play, as well as the multiple factors that impact the types of risks they might face, need to be taken into account by international partners from the very beginning of a partnership.Building Holistic Security: Addressing Security Risks of Women Peacebuilders Through Partnerships addresses how international partners who wish to work with women peacebuilders and support them in addressing the risks and insecurity they face need to recognize the scope and nature of peacebuilding work, which is often cross-cutting, overlapping with humanitarian response and development work. Understanding the nuances and breadth of women peacebuilders’ work is crucial to identifying the risks they face and providing them with effective legal, political and financial protection — and is thereby essential to creating partnerships that mitigate and address these risks. Through case studies, the report identifies challenges and opportunities drawn directly from the lived realities of women peacebuilders and their partners, as well as from experts working in the Women, Peace and Security field.https://digital.sandiego.edu/ipj-research/1072/thumbnail.jp

A custom-made electronic dynamometer for evaluation of peak ankle torque after COVID-19

The negative effects of SARS-CoV-2 infection on the musculoskeletal system include symptoms of fatigue and sarcopenia. The aim of this study is to assess the impact of COVID-19 on foot muscle strength and evaluate the reproducibility of peak ankle torque measurements in time by using a custom-made electronic dynamometer. In this observational cohort study, we compare two groups of four participants, one exposed to COVID-19 throughout measurements and one unexposed. Peak ankle torque was measured using a portable custom-made electronic dynamometer. Ankle plantar flexor and dorsiflexor muscle strength was captured for both feet at different ankle angles prior and post COVID-19. Average peak torque demonstrated no significant statistical differences between initial and final moment for both groups (p = 0.945). An increase of 4.8%, p = 0.746 was obtained in the group with COVID-19 and a decrease of 1.3%, p = 0.953 was obtained in the group without COVID-19. Multivariate analysis demonstrated no significant differences between the two groups (p = 0.797). There was a very good test–retest reproducibility between the measurements in initial and final moments (ICC = 0.78, p < 0.001). In conclusion, peak torque variability is similar in both COVID-19 and non-COVID-19 groups and the custom-made electronic dynamometer is a reproducible method for repetitive ankle peak torque measurements

Building Holistic Security: Addressing Security Risks of Women Peacebuilders Through Partnerships

Women peacebuilders inevitably face risks and insecurity in their daily work. International partners have an important role to play in supporting their safety and protection. Understanding women peacebuilders’ roles and the types of risks they face is the first step in ensuring an adequate response. The diversity of roles that women peacebuilders play, as well as the multiple factors that impact the types of risks they might face, need to be taken into account by international partners from the very beginning of a partnership.Building Holistic Security: Addressing Security Risks of Women Peacebuilders Through Partnerships addresses how international partners who wish to work with women peacebuilders and support them in addressing the risks and insecurity they face need to recognize the scope and nature of peacebuilding work, which is often cross-cutting, overlapping with humanitarian response and development work. Understanding the nuances and breadth of women peacebuilders’ work is crucial to identifying the risks they face and providing them with effective legal, political and financial protection — and is thereby essential to creating partnerships that mitigate and address these risks. Through case studies, the report identifies challenges and opportunities drawn directly from the lived realities of women peacebuilders and their partners, as well as from experts working in the Women, Peace and Security field.https://digital.sandiego.edu/ipj-research/1071/thumbnail.jp

Liposomal sunitinib for ocular drug delivery : A potential treatment for choroidal neovascularization

Choroidal neovascularization (CNV) is a prevalent vision-threatening vascular disorder in aging population. CNV is associated with several diseases in the posterior segment of the eye such as age-related macular degeneration (AMD). In this study we developed sunitinib-loaded liposomes to block the neovascularization signalling pathway through inhibition of tyrosine kinase of vascular endothelial growth factor receptors (VEGFRs). Liposomal sunitinib formulations were prepared by thin film hydration method and studied for their encapsulation efficiency (EE), loading capacity (LC) and drug release profile in buffer andvitreous. Our finding showed that the liposomes (mean size 104 nm) could effectively entrap sunitinib (EE approximate to 95%) at relatively high loading capacity (LC approximate to 5%) and release sunitinib over at least 3 days. Intravitreal sunitinib-loaded liposomes revealed inhibitory effect on established neovascularization in laser-induced CNV mouse model while the intravitreal injection of sunitinib solubilized with cyclodextrin was inefficient in management of neovascularization. Accordingly, liposomal sunitinib is a promising drug delivery system that should be further studied to inhibit the CNV related to AMD.Peer reviewe