Comparing calf sex ratio and semen sex ratio determined by conventional PCR

Thirty-nine ejaculates from 8 Holstein bulls were collected. Straws of semen (n=750) were distributed among dairies in three states. Ten straws per ejaculate were sent to Louisiana State University (LSU) Dairy Improvement Center for conventional polymerase chain reaction (PCR) analysis. Spermatozoal DNA was extracted and PCR analysis was done using one primer set amplifying a single copy 125 base pair (bp) section of the Bos taurus factor IX (Christmas factor) precursor (found on the X chromosome) and another primer set amplifying a single copy section of the Bos taurus sex determining region Y protein (SRY) gene (found on the Y chromosome). A 294 bp product from the Bos taurus glyceraldehydes-phosphate-dehydrogenase (GAPDH) was amplified as an internal control. Standard curves were designed using PCR products in known ratios. Gel electrophoresis and image analysis allowed for determination of predicted % Y chromosome-bearing spermatozoa (predicted % Y spermatozoa). Calf sex was reported and % male calves was determined between bull, ejaculate within bull, state, and location within state. Predicted % Y spermatozoa and % male calves showed significant correlation to each other. No significant variance between bull was found in predicted % Y spermatozoa or % male calves, but significant variance was found between ejaculate within bull for both. PCR technology used for determining the % Y spermatozoa in ejaculates was shown to be an adequate method to determine semen sex ratio

Adaptive radiotherapy for bladder cancer – a systematic review

Radiotherapy has been offered as a multi-modality treatment for bladder cancer patients. Due to the significant variation of bladder volume observed throughout the course of treatment, large margins in the range of 20 – 30 mm have been used, unnecessarily irradiating a large volume of normal tissue. With the capability of visualizing soft tissue in Cone Beam Computerized Tomography, there is opportunity to modify or to adapt the plan based on the variation observed during the course of treatment for quality improvement. A literature search was conducted in May 2016, with the aim of examining the adaptive strategies that have been developed for bladder cancer and assessing the efficacy in improving treatment quality. Among the 18 identified publications, three adaptive strategies were reported: Plan of the Day, patient-specific planning target volume and daily reoptimization. Overall, any of the adaptive strategies achieved a significant improvement in reducing the irradiated volume compared to the non-adaptive approach, outweighing the additional resource required for its execution. The amount and the type of resource required vary from strategy to strategy, suggesting the need for the individual institution to assess feasibility based on the existing infrastructure in order to identify the most appropriate strategy for implementation

Three- versus four-factor prothrombin complex concentrate for the reversal of warfarin-induced bleeding

Objective: The objective of this study was to evaluate the effectiveness of 3-factor prothrombin complex concentrate (3F-PCC) compared to 4-factor PCC (4F-PCC) in warfarin-associated bleeding. Methods: This multicenter, retrospective, cohort study analyzed data from patients admitted between May 2011 and October 2014 who received PCC for warfarin-associated bleeding. The primary outcome was the rate of international normalized ratio (INR) normalization, defined as an INR ≤1.3, after administration of 3F-PCC compared to 4F-PCC. Other variables of interest included the incidence of additional reversal agents, new thromboembolic events, and mortality. Results: A total of 134 patients were included in the analysis. The average dose of PCC administered was 24.6 ± 9.3 units/kg versus 36.3 ± 12.8 units/kg in the 3F-PCC and 4F-PCC groups, respectively, P < 0.001. Baseline INR in the 3F-PCC and 4F-PCC groups was 3.61 ± 2.3 and 6.87 ± 2.3, respectively P < 0.001. 4F-PCC had a higher rate of INR normalization at first INR check post-PCC administration compared to 3F-PCC (84.2% vs. 51.9%, P = 0.0001). Thromboembolic events, intensive care unit and hospital length of stay, and mortality were similar among both groups. Conclusion: The use of 4F-PCC leads to a more significant reduction in INR compared to 3F-PCC though no difference in mortality or length of stay was observed. Thromboembolism rates were similar among both groups

Probabilistic modeling of one dimensional water movement and leaching from highway embankments containing secondary materials

Predictive methods for contaminant release from virgin and secondary road construction materials are important for evaluating potential long-term soil and groundwater contamination from highways. The objective of this research was to describe the field hydrology in a highway embankment and to investigate leaching under unsaturated conditions by use of a contaminant fate and transport model. The HYDRUS2D code was used to solve the Richards equation and the advection–dispersion equation with retardation. Water flow in a Minnesota highway embankment was successfully modeled in one dimension for several rain events after Bayesian calibration of the hydraulic parameters against water content data at a point 0.32 m from the surface of the embankment. The hypothetical leaching of Cadmium from coal fly ash was probabilistically simulated in a scenario where the top 0.50 m of the embankment was replaced by coal fly ash. Simulation results were compared to the percolation equation method where the solubility is multiplied by the liquid-to-solid ratio to estimate total release. If a low solubility value is used for Cadmium, the release estimates obtained using the percolation/equilibrium model are close to those predicted from HYDRUS2D simulations (10–4–10–2 mg Cd/kg ash). If high solubility is used, the percolation equation over predicts the actual release (0.1–1.0 mg Cd/kg ash). At the 90th percentile of uncertainty, the 10-year liquid-to-solid ratio for the coal fly ash embankment was 9.48 L/kg, and the fraction of precipitation that infiltrated the coal fly ash embankment was 92%. Probabilistic modeling with HYDRUS2D appears to be a promising realistic approach to predicting field hydrology and subsequent leaching in embankments

Examining Incidents of Sexual Misconduct Reported to Title IX Coordinators: Results from New York’s Institutions of Higher Education

A paucity of studies has examined incidents of sexual misconduct reported to Title IX coordinators at institutions of higher education (IHEs) or examined differences across types of IHEs. We used 2018 data from incidents of sexual misconduct (N = 3,829) reported to Title IX coordinators at IHEs in New York (N = 209) to examine the context, processes, and outcomes of reported incidents. Findings show that most incidents reported to Title IX coordinators did not prompt the IHE’s conduct process; “other” disciplinary sanctions were the favored response for responsible students, while suspensions and/or expulsions were rarely used. Further data collection and analysis is needed to understand victim and IHE decision-making regarding reported incidents of sexual misconduct and specific processes at community colleges and independent IHEs

The Carbon Storage Future of Public Lands

To meet the climate and energy goals set forth by the Biden Administration and the Paris Agreement, the United States must dramatically reduce carbon emissions. Use of public lands for carbon dioxide removal activities, including carbon capture, utilization, and storage (CCUS), has the potential to advance carbon reduction goals and concurrently provide economic revitalization opportunities to communities dependent on fossil industries. Current federal law presents numerous challenges and opportunities associated with utilization of federal pore space for CCUS. Although federal grant programs and tax incentives encourage deployment of CCUS technologies, legal and land-management issues related to public lands have received comparatively little legislative or agency attention. This essay seeks to bring attention to land-management aspects of geologic storage and to broaden conversations regarding CCUS technology deployment on federal lands. The authors identify opportunities for courts, agencies, and Congress to address uncertainties related to federal pore space and promote cooperation and coordination with state agencies

Comparison of 3 image-guided adaptive strategies for bladder locoregional radiotherapy

The objective of this study was to compare the dosimetric differences of a population-based planning target volume (PTV) approach and 3 proposed adaptive strategies: plan of the day (POD), patient-specific PTV (PS-PTV), and daily reoptimization (ReOpt). Bladder patients (n = 10) were planned and treated to 46 Gy in 23 fractions with a full bladder in supine position by the standard strategy using a population-based PTV. For each patient, the adaptive strategy was executed retrospectively as follows: (1) POD—multiple distributions of various PTV sizes were generated, and the appropriate distribution based on the bladder of the day was selected for each fraction; (2) PS-PTV—population-based PTV was used for the first 5 fractions and a new PTV derived using information from these fractions was used to deliver the remaining 18 fractions; and (3) ReOpt—distribution was reoptimized for each fraction based on the bladder of the day. Daily dose was computed on all cone beam computed tomographies (CBCTs) and deformed back to the planning computed tomography (CT) for dose summation afterward. V95_Accu, the volume receiving an accumulated delivered dose of 43.7 Gy (95% prescription dose), was measured for comparison. Mean V95_Accu (cm3) values were 1410 (standard deviation [SD]: 227), 1212 (SD: 186), 1236 (SD: 199), and 1101 (SD: 180) for standard, POD, PS-PTV, and ReOpt, respectively. All adaptive strategies significantly reduced the irradiated volume, with ReOpt demonstrating the greatest reduction compared with the standard (− 25%), followed by PS-PTV (− 16%) and POD (− 12%). The difference in the magnitude of reduction between ReOpt and the other 2 strategies reached statistical significance (p = 0.0006). ReOpt is the best adaptive strategy at reducing the irradiated volume because of its frequent adaptation based on the daily geometry of the bladder. The need to adapt only once renders PS-PTV to be the best alternative adaptive strategy