Comparison of Compliance with Cervical Cancer Screening among Women aged 18 and above in Arkansas and the United States

Introduction: Cervical cancer preys on women without access to preventative screening. Any woman who has developed an invasive case of cervical cancer should be regarded as a failure of screening. Disparities in access to screening, triage, and treatment fuel an uneven distribution in the burden of cervical cancer within the United States; weighing unusually heavy on the Southern States like Arkansas. The purpose of this study was to evaluate demographic and behavioral risk factors for cervical cancer and identify potential barriers which may influence the likelihood of complying with current Pap test recommendations on a state level, in Arkansas, and also a national scale, in the United States. Materials and Methods: National data from the 2014 Behavioral Risk Factor Survey System (BRFSS) was used to collect demographic characteristics and behavioral risk factors among females aged 18 and above in Arkansas and the United States. BFRSS data included a total of 1,587 women in Arkansas and 162,222 in the United States who were aged 18 and above and eligible to receive Pap tests. Women with a medical history of hysterectomy that included the removal of the cervix were excluded from analysis because they are not considered to be medically eligible to receive Pap tests. Results: Socioeconomic status, age, race, and health insurance were significantly associated with likelihood to comply with current screening recommendations among women in both Arkansas and the United States. Arkansan women who had less than a 12-year education (Odds Ratio (OR) = 1.41, 95% Confidence Interval (CI) = 1.04, 1.64) and were without health insurance (OR = 3.56, 95% CI = 1.66, 7.66) were more likely to be non-compliant with Pap testing, which is similar to the finding on the national level. The prevalence of Pap test compliance was significantly lower among women in Arkansas when compared to US average in every sociodemographic sector. Discussion: According to US Census data collected in 2013, Arkansas had the highest cervical cancer incidence of 10.6 per 100,000 person-years, while the national average was 7.7 per 100,000 person-years. Therefore, future population-based cervical cancer interventions in Arkansas should target the promotion of Pap test compliance among the most vulnerable subpopulations (i.e., low socioeconomic status, minority, medically under or uninsured). Organized screening interventions might include offering free or reduced cost Pap testing in both urban and rural locations

Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues

Due to the retina’s inability to replace photoreceptors lost during retinal degeneration, significant interest has been placed in methods to implant replacement cells. Polymer scaffolds are increasingly being studied as vehicles for cellular delivery to degenerated retinas. Previously, we fabricated poly(methyl methacrylate) thin film scaffolds that increased survival and integration of implanted retinal progenitor cells (RPCs). Additionally, these scaffolds minimized the trauma and cellular response associated with implantation of foreign bodies into mouse eyes. Here, we demonstrate that biodegradable polycaprolactone (PCL) thin film scaffolds can be fabricated with integrated microtopography. Microfabricated topography in a PCL thin film enhanced the attachment and organization of RPCs compared to unstructured surfaces. Using real-time quantitative polymerase chain reaction we also observed that attachment to microtopography induced cellular differentiation. RPCs grown on PCL thin films exhibited an increase in gene expression for the photoreceptor markers recoverin and rhodopsin, an increase in the glial and Müller cell marker GFAP, and a decrease in SOX2 gene expression (a marker for undifferentiated progenitor cells) compared to cells grown on unmodified tissue culture polystyrene (TCPS)

Who Needs Crossings? Hardness of Plane Graph Rigidity

We exactly settle the complexity of graph realization, graph rigidity, and graph global rigidity as applied to three types of graphs: "globally noncrossing" graphs, which avoid crossings in all of their configurations; matchstick graphs, with unit-length edges and where only noncrossing configurations are considered; and unrestricted graphs (crossings allowed) with unit edge lengths (or in the global rigidity case, edge lengths in {1,2}). We show that all nine of these questions are complete for the class Exists-R, defined by the Existential Theory of the Reals, or its complement Forall-R; in particular, each problem is (co)NP-hard. One of these nine results - that realization of unit-distance graphs is Exists-R-complete - was shown previously by Schaefer (2013), but the other eight are new. We strengthen several prior results. Matchstick graph realization was known to be NP-hard (Eades & Wormald 1990, or Cabello et al. 2007), but its membership in NP remained open; we show it is complete for the (possibly) larger class Exists-R. Global rigidity of graphs with edge lengths in {1,2} was known to be coNP-hard (Saxe 1979); we show it is Forall-R-complete. The majority of the paper is devoted to proving an analog of Kempe\u27s Universality Theorem - informally, "there is a linkage to sign your name" - for globally noncrossing linkages. In particular, we show that any polynomial curve phi(x,y)=0 can be traced by a noncrossing linkage, settling an open problem from 2004. More generally, we show that the nontrivial regions in the plane that may be traced by a noncrossing linkage are precisely the compact semialgebraic regions. Thus, no drawing power is lost by restricting to noncrossing linkages. We prove analogous results for matchstick linkages and unit-distance linkages as well

Finding a Hamiltonian Path in a Cube with Specified Turns is Hard

We prove the NP-completeness of finding a Hamiltonian path in an N × N × N cube graph with turns exactly at specified lengths along the path. This result establishes NP-completeness of Snake Cube puzzles: folding a chain of N3 unit cubes, joined at face centers (usually by a cord passing through all the cubes), into an N × N × N cube. Along the way, we prove a universality result that zig-zag chains (which must turn every unit) can fold into any polycube after 4 × 4 × 4 refinement, or into any Hamiltonian polycube after 2 × 2 × 2 refinement

Folding equilateral plane graphs

22nd International Symposium, ISAAC 2011, Yokohama, Japan, December 5-8, 2011. ProceedingsWe consider two types of folding applied to equilateral plane graph linkages. First, under continuous folding motions, we show how to reconfigure any linear equilateral tree (lying on a line) into a canonical configuration. By contrast, such reconfiguration is known to be impossible for linear (nonequilateral) trees and for (nonlinear) equilateral trees. Second, under instantaneous folding motions, we show that an equilateral plane graph has a noncrossing linear folded state if and only if it is bipartite. Not only is the equilateral constraint necessary for this result, but we show that it is strongly NP-complete to decide whether a (nonequilateral) plane graph has a linear folded state. Equivalently, we show strong NP-completeness of deciding whether an abstract metric polyhedral complex with one central vertex has a noncrossing flat folded state with a specified “outside region”. By contrast, the analogous problem for a polyhedral manifold with one central vertex (single-vertex origami) is only weakly NP-complete

Second-scale rotational coherence and dipolar interactions in a gas of ultracold polar molecules

Ultracold polar molecules combine a rich structure of long-lived internal states with access to controllable long-range anisotropic dipole–dipole interactions. In particular, the rotational states of polar molecules confined in optical tweezers or optical lattices may be used to encode interacting qubits for quantum computation or pseudo-spins for simulating quantum magnetism. As with all quantum platforms, the engineering of robust coherent superpositions of states is vital. However, for optically trapped molecules, the coherence time between rotational states is typically limited by inhomogeneous differential light shifts. Here we demonstrate a rotationally magic optical trap for 87Rb133Cs molecules that supports a Ramsey coherence time of 0.78(4) s in the absence of dipole–dipole interactions. This is estimated to extend to >1.4 s at the 95% confidence level using a single spin-echo pulse. In our trap, dipolar interactions become the dominant mechanism by which Ramsey contrast is lost for superpositions that generate oscillating dipoles. By changing the states forming the superposition, we tune the effective dipole moment and show that the coherence time is inversely proportional to the strength of the dipolar interaction. Our work unlocks the full potential of the rotational degree of freedom in molecules for quantum computation and quantum simulation

A Digital Atlas to Characterize the Mouse Brain Transcriptome

Massive amounts of data are being generated in an effort to represent for the brain the expression of all genes at cellular resolution. Critical to exploiting this effort is the ability to place these data into a common frame of reference. Here we have developed a computational method for annotating gene expression patterns in the context of a digital atlas to facilitate custom user queries and comparisons of this type of data. This procedure has been applied to 200 genes in the postnatal mouse brain. As an illustration of utility, we identify candidate genes that may be related to Parkinson disease by using the expression of a dopamine transporter in the substantia nigra as a search query pattern. In addition, we discover that transcription factor Rorb is down-regulated in the barrelless mutant relative to control mice by quantitative comparison of expression patterns in layer IV somatosensory cortex. The semi-automated annotation method developed here is applicable to a broad spectrum of complex tissues and data modalities

The presence of prolines in the flanking region of an immunodominant HIV-2 gag epitope influences the quality and quantity of the epitope generated

Both the recognition of HIV‐infected cells and the immunogenicity of candidate CTL vaccines depend on the presentation of a peptide epitope at the cell surface, which in turn depends on intracellular antigen processing. Differential antigen processing maybe responsible for the differences in both the quality and the quantity of epitopes produced, influencing the immunodominance hierarchy of viral epitopes. Previously, we showed that the magnitude of the HIV‐2 gag‐specific T‐cell response is inversely correlated with plasma viral load, particularly when responses are directed against an epitope, 165DRFYKSLRA173, within the highly conserved Major Homology Region of gag‐p26. We also showed that the presence of three proline residues, at positions 119, 159 and 178 of gag‐p26, was significantly correlated with low viral load. Since this proline motif was also associated with stronger gag‐specific CTL responses, we investigated the impact of these prolines on proteasomal processing of the protective 165DRFYKSLRA173 epitope. Our data demonstrate that the 165DRFYKSLRA173 epitope is most efficiently processed from precursors that contain two flanking proline residues, found naturally in low viral‐load patients. Superior antigen processing and enhanced presentation may account for the link between infection with HIV‐2 encoding the “PPP‐gag” sequence and both strong gag‐specific CTL responses as well as lower viral load

The presence of prolines in the flanking region of an immunodominant HIV-2 gag epitope influences the quality and quantity of the epitope generated

Both the recognition of HIV‐infected cells and the immunogenicity of candidate CTL vaccines depend on the presentation of a peptide epitope at the cell surface, which in turn depends on intracellular antigen processing. Differential antigen processing maybe responsible for the differences in both the quality and the quantity of epitopes produced, influencing the immunodominance hierarchy of viral epitopes. Previously, we showed that the magnitude of the HIV‐2 gag‐specific T‐cell response is inversely correlated with plasma viral load, particularly when responses are directed against an epitope, 165DRFYKSLRA173, within the highly conserved Major Homology Region of gag‐p26. We also showed that the presence of three proline residues, at positions 119, 159 and 178 of gag‐p26, was significantly correlated with low viral load. Since this proline motif was also associated with stronger gag‐specific CTL responses, we investigated the impact of these prolines on proteasomal processing of the protective 165DRFYKSLRA173 epitope. Our data demonstrate that the 165DRFYKSLRA173 epitope is most efficiently processed from precursors that contain two flanking proline residues, found naturally in low viral‐load patients. Superior antigen processing and enhanced presentation may account for the link between infection with HIV‐2 encoding the “PPP‐gag” sequence and both strong gag‐specific CTL responses as well as lower viral load