159 research outputs found
Pengaruh Pemulihan Jasa Terhadap Kepercayaan Merek Hotel Di Indonesia Dengan Kepuasan Atas Pemulihan Jasa Sebagai Variabel Perantara
Penelitian ini dilakukan untuk mengetahui pengaruh penerapan pemulihan jasa (perceived justice) pada hotel bintang 1-3 di Indonesia atas kegagalan jasa terhadap kepercayaan merek (brand reliability dan brand intention) dengan menggunakan kepuasan atas pemulihan jasa sebagai variabel intervening. Teknik analisa data yang digunakan adalah PLS-SEM. Hasil penelitian menunjukkan bahwa pemulihan jasa memiliki pengaruh yang positif terhadap kepuasan atas pemulihan jasa. Sementara itu kepuasan atas pemulihan jasa terbukti memiliki pengaruh yang positif dan signifikan dalam mengembalikan kepercayaan merek serta menjadi variabel intervening yang positif dan signifikan bagi hubungan antara kepuasan atas pemulihan jasa dan kepercayaan merek. This study was conducted to determine the impact of service recovery (perceived justice) at 1-3 star hotel in Indonesia towards brand trust and satisfaction of service recovery as the intervening variable. Data analysis technique used is PLS-SEM. The predictive used to add knowledge to the community, as well as for the hotel management to pay more attention about implication of service recovery which affect the guest satisfaction to rebuild brand trust. The results shown that service recovery has the positive impact to satisfaction of service recovery thus, only procedural justice that have the insignificant impact. Furthermore, satisfaction of service recovery has the positive and significant impact towards brand trust and became a positive and significant intervening variable for the correlation between service recovery and brand trust
Factors associated with chronic kidney disease in patients with type 2 diabetes in Bangladesh
Diabetes and chronic kidney disease (CKD) are a major public health burden in low- and middle-income countries. This study aimed to explore factors associated with CKD in patients with type 2 diabetes (T2D) in Bangladesh. A cross-sectional study was conducted among 315 adults with T2D presenting at the outpatient department of Bangladesh Institute of Health Sciences (BIHS) hospital between July 2013 to December 2013. CKD was diagnosed based on the estimated glomerular filtration rate using the ‘Modification of Diet in Renal Disease’ equations and the presence of albu-minuria estimated by the albumin-to-creatinine ratio. Multivariate logistic regression analysis was used to determine the factors associated with CKD. The overall prevalence of CKD among patients with T2D was 21.3%. In the unadjusted model, factors associated with CKD included age 40–49 years (OR: 5.7, 95% CI: 1.3–25.4), age 50–59 years (7.0, 1.6–39), age ≥60 years (7.6, 1.7–34), being female (2.2, 1.2–3.8), being hypertensive (1.9, 1.1–3.5), and household income between 10,001 and 20,000 Bangladeshi taka, BDT (2.9, 1.0–8.2) compared with income ≤10,000 BDT. However, after ad-justment of other covariates, only the duration of hypertension and household income (10,001– 20,000 BDT) remained statistically significant. There is a need to implement policies and programs for early detection and management of hypertension and CKD in T2D patients in Bangladesh. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. **Please note that there are multiple authors for this article therefore only the name of the first 5 including Federation University Australia affiliate “Masudus Salehin” is provided in this record*
MANAJEMEN PELAKSANAAN PAMERAN STUDI KHUSUS MAHASISWA PENDIDIKAN SENI RUPA UNIVERSITAS MUHAMMADIYAH MAKASSAR
, PANGERAN PAITA YUNUS
Pendidikan Seni Rupa, Universitas Negeri Makassar
Email: [email protected]
Abstract : The study aims at examining (1) the management of the implementation
of exhibition special study of students of Fine Arts Education in Universitas
Muhammadiyah Makassar, (2) the supporting and inhibiting factors of the
management of the implementation of exhibition special study of students of Fine
Arts Education in Universitas Muhammadiyah Makassar.
The study is a survey research with descriptive qualitative. Data were
collected by employing observation conducted by observing and taking notes
systematically on the phenomenon; interview by collecting the data where the
researcher conducted question and answer directly to the respondents; and
documentation by collecting the archives and pictures to support the data. The
research subjects were the lecture of Exhibition Course, curator, students, and
appreciator. The research objects were (1) the management of the implementation of
exhibition special study of students of Fine Arts Education in Universitas
Muhammadiyah Makassar (2) the supporting and inhibiting factors of the
management of the implementation of exhibition special study of students of Fine
Arts Education in Universitas Muhammadiyah Makassar.
The result of the study reveal that (1) the management of the implementation
of exhibition special study of students of Fine Arts Education in Universitas
Muhammadiyah Makassar among others are (a) work process, (b) the concept of
curation, (c) exhibition activity theme, (d) correspondence, (e) form a committe or
team work, (f) publication and promotion, (g) budget, (h) work display and
arrangement of exhibition space, (i) opening ceremony and exhibition (j) evaluation;
(2) the supporting factors in management of the implementation of exhibition special
study of students of Fine Arts Education in Universitas Muhammadiyah Makassar are
(a) performance art and (b) artist talk; whereas, the inhibiting factors of management
of the implementation of exhibition are (a) non-conductive and strategic place as a
reference for exhibition activity and (b) inadequate facility and infrastructure
EFFECTS OF DIFFERENT CHEMICAL PRETREATMENTS ON CELL WALL COMPOSITION AND ASH CONCENTRATION OF SWEET SORGHUM BAGASSE FOR BIOETHANOL PRODUCTION
Pretreatment is one of the key processes in lignocellulosic bioethanol production, which is needed to improve accessibility of enzymes to cellulose. This study was conducted to investigate the effects of different chemical pretreatments on cell wall composition and ash concentration of sweet sorghum bagasse. 9 different pretreatment methods used in the study can be categorized into 3 different methods such as dilute sulphuric acid (1, 1.5 and 2 % H2SO4 w/v), dilute sodium hydroxide (1, 1.5 and 2 % NAOH w/v) and sequential dilute sulphuric acid and sodium hydroxide (1 % H2SO4 w/v + 0.5 M NAOH, 1.5 % H2SO4 w/v + 0.5 M NAOH and 2 % H2SO4 w/v + 0.5 M NAOH). According to results, while 2 % H2SO4 w/v + 0.5 M NAOH gave the highest cellulose (91.51 %) and lowest lignin (1.7 %) concentrations, the lowest cellulose (65.11 %), hemicellulose (0.4 %), and highest lignin concentrations (23.42 %) were provided by 1.5 % H2SO4 w/v among pretreatments. Cellulose, hemicellulose and lignin contents of sweet sorghum bagasse after sodium hydroxide pretreatments ranged from 76.72 to 79.88, 11.75 to 14.62, and 2.05 to 4.11 %, respectively. The most appropriate cell wall composition for enzymatic hydrolysis was derived from sequential dilute sulphuric acid and sodium hydroxide pretreatments due to the fact that they provided the highest cellulose (90.68 – 91.51 %), lowest lignin (1.7 – 3.41 %) and desirable hemicellulose (1.10 – 1.82 %) contents. However, enzymatic hydrolysis must be done to learn which method enables the highest fermentable sugar production
Elucidating the Modes of Action of the Antitumour New Zealand Marine Natural Product Pterocellin A
Pterocellin A is a novel bioactive alkaloid isolated from the New Zealand marine bryozoan Pterocella vesiculosa. It exhibits potent antitumour activity towards the P388 (murine leukaemia) cell line in vitro with an IC₅₀ value of 477 ng/mL. Further screening at the NCI indicated that it was selectively sensitive towards certain non-small cell lung, melanoma, and breast cancer cell lines, however, the biological mode of action of pterocellin A is unknown.
This project investigated and elucidated the cytotoxicity of pterocellin A using the human cervical cancer cell line HeLa. Cellular toxicity can be manifested as either necrotic or apoptotic cell death. Cancer cells are prone to resisting apoptosis, and one of the key mechanisms recognised in anticancer agents is the induction of apoptosis. Pterocellin A exhibited similar cytotoxicity against the HeLa cell line compared to P388 cells with an IC₅₀ value of 886 ng/mL. In this study, HeLa cells were incubated with various concentrations (up to 2000 ng/mL) of pure pterocellin A, and characteristic markers of apoptotic and necrotic cell death were investigated using several in vitro biochemistry techniques. Assessments were made on the morphological and biochemical changes in HeLa cells after treatment with pterocellin A.
Time-course MTT and LDH assays were carried out and the results showed that only a low level of cytosolic LDH was detected in the supernatant after all the cells had died from pterocellin A treatment at 2000 ng/mL. This indicated the cells maintained membrane integrity upon death which suggested apoptosis. Additionally, morphological changes were observed under the microscope after six hours of treatment. Cell shrinkage and nucleus condensation were observed, as well as apparent membrane blebbing, a key feature of apoptosis. The MTT data were also indicative of mitochondria impairment which could suggest that pterocellin A targets the mitochondria. This idea was supported by the observed changes in the morphology and location of the mitochondria after exposure to pterocellin A. Furthermore, the level of activated caspase-3 in HeLa cells increased after treatment with pterocellin A; activated caspase-3 can only be detected after a series of signalling events following the induction of apoptosis. These data support the notion that pterocellin A is an inducer of apoptosis in HeLa cells.
This project also utilised the biochemical assays to develop a systematic bioassay screening system at the University of Waikato, and further investigated the bioactive metabolites of P. vesiculosa. The same approach taken to study pure pterocellin A in HeLa cells was successfully applied to the bioassay development. The bioactive fractions separated from the crude extract were analysed by LC-MS and several unknown metabolites were observed, however due to time constraints associated with the project, no further investigation was carried out
Interaction of human dipeptidyl peptidase IV and human immunodeficiency virus type-1 transcription transactivator in Sf9 cells
<p>Abstract</p> <p>Background</p> <p>Dipeptidyl peptidase IV (DPPIV) also known as the T cell activation marker CD26 is a multifunctional protein which is involved in various biological processes. The association of human-DPPIV with components of the human immunodeficiency virus type-1 (HIV1) is well documented and raised some discussions. Several reports implicated the interaction of human-DPPIV with the HIV1 transcription transactivator protein (HIV1-Tat) and the inhibition of the dipeptidyl peptidase activity of DPPIV by the HIV1-Tat protein. Furthermore, enzyme kinetic data implied another binding site for the HIV1-Tat other than the active centre of DPPIV. However, the biological significance of this interaction of the HIV1-Tat protein and human-DPPIV has not been studied, yet. Therefore, we focused on the interaction of HIV1-Tat protein with DPPIV and investigated the subsequent biological consequences of this interaction in <it>Spodoptera frugiperda </it>cells, using the BAC-TO-BAC baculovirus system.</p> <p>Results</p> <p>The HIV1-Tat protein (Tat-<it>BRU</it>) co-localized and co-immunoprecipitated with human-DPPIV protein, following co-expression in the baculovirus-driven <it>Sf9 </it>cell expression system. Furthermore, tyrosine phosphorylation of DPPIV protein was up-regulated in Tat/DPPIV-co-expressing cells after 72 h culturing and also in DPPIV-expressing <it>Sf9 </it>cells after application of purified recombinant Tat protein. As opposed to the expression of Tat alone, serine phosphorylation of the Tat protein was decreased when co-expressed with human-DPPIV protein.</p> <p>Conclusions</p> <p>We show for the first time that human-DPPIV and HIV1-Tat co-immunoprecipitate. Furthermore, our findings indicate that the interaction of HIV1-Tat and human-DPPIV may be involved in signalling platforms that regulate the biological function of both human-DPPIV and HIV1-Tat.</p
Seismotectonics and landslides of the Crati Graben (Calabrian Arc, Southern Italy)
The Crati Graben is a depression of Plio-Holocene age mainly controlled by extensional N–S striking faults and WNW–ESE transcurrent faults, in its northern and southern extremity. It is characterized by high landslide susceptibility due to the particular geo-structural pattern and seismotectonic characters. Landslides involve many villages, infrastructure and food crops, bringing serious economic and social damage. The seismotectonic and landslides Main Map of the Crati Graben, described in this paper, represents an update in detail of the framework of landslide risk areas and shows the main active and recent faults of the Crati Graben. The landslides and the faults, have been identified and classified, originally at detail scale (1:5000) and, then, represented at 1:50,000 scale. The geo-structural and geomorphological data were analysed in a geographic information system. The work has revealed a correlation between the trend of faults with respect to the distribution of landslides and of the historical and instrumental seismicity. The work presents an updated knowledge framework of risk conditions of the study zone, where risk areas related to slope instability are hierarchically classified according to the destructive potential of landslides. This document may be therefore a useful reference in planning and prioritising in the design of interventions for the safety of slopes and waterways
Traumatic Healing Program for Elementary School Student in Sumberdadi Village Post Online Learning During Pandemic Covid-19
Pandemic covid 19 has brought about big impact to all sectors including education. The online learning during pandemic brought great impact to the psychology of the elementary school student. In addition, it also changed the student habit. The government policy to conduct limited offline meeting can not make students directly go to school and receive the material from the teacher. This psychological condition must be prepared so that the students can and enjoy and follow the learning maximally. This preparation is made through traumatic healing program in the form of fun learning which can boost student’s literacy, sport and skill. This program used service-learning method and involved students and Sumberdadi people as well. The target of this program is elementary school student from Sumberdadi village. This program was held for two months. The result of this program showed that students were enthusiastic and full of spirit to join the program. Besides, the no longer complained about the activity of doing assignment from schools. It can be concluded that the students are ready to join limited offline meeting at schoo
PENGARUH PEMULIHAN JASA TERHADAP KEPUASAN DAN KEPERCAYAAN PELANGGAN ATAS MEREK HOTEL: STUDI PADA HOTEL BERBINTANG SATU SAMPAI TIGA DI INDONESIA
Penelitian ini dilakukan untuk menganalisa penerapan pemulihan jasa di hotel berbintang satu sampai tiga di Indonesia atas kegagalan jasa yang terjadi dan pengaruhnya terhadap kepercayaan pelanggan atas merek hotel. Survei dilakukan kepada 286 responden yang menginap di hotel dan melakukan keluhan akibat kegagalan jasa yang dialami selama menginap. Data primer diolah dengan teknik statistik SEM-PLS. Hasil penelitian menunjukkan bahwa pemulihan jasa memiliki pengaruh yang positif terhadap kepuasan atas pemulihan jasa. Sementara itu, kepuasan atas pemulihan jasa terbukti memiliki pengaruh yang positif dan signifikan dalam mengembalikan kepercayaan responden atas merek hotel serta menjadi variabel mediasi yang signifikan bagi hubungan antara kepuasan atas pemulihan jasa dan kepercayaan merek
Potential, cultivation and quality of some crambe sp. in Southern Turkey
Crambe sp. is an oilseed crop
from the Brassicae family and native to the
Mediterranean region. It can be converted
into a number of industrial and energy uses.
Crambe oil is used in introducing in stain,
primers, plastic and solid wax, cosmetic and
engine portions in the form of nylon-13.13
exclude carburetor as eco-friendly. All these
properties make it interesting. This study is
the first report on yield, cultivation
procedure, and quality characteristics of
Crambe sp. cultivated in Turkey. Native
Crambe seeds, collected from eight different
locations in Turkey, were cultivated under
Çukurova conditions in Mediterranean
region. Two Crambe species, Crambe
orientalis and Crambe tataria, determined
at the locations were studied, and some
morphological characteristics and oil
compositions were sown from cultivars and
native forms. Fatty acid composition of
seeds was examined with GC and GC/MS.
In native populations, high seed oil contents
were obtained from C. tataria (Ankara -
Faculty of Science and Letters) and
C. tataria (Kahramanmaraş-Elbistan), as
45.62 and 45.50%, respectively. The highest
erucic acid content (49.0 %) was found in
C. tataria (Ankara-Bilkent). In Çukurova
conditions, despite cultivated all collected
species, just C. orientalis was bloom among
this species, and so that seed yield (472.77
kg/ha) oil rate (% 27.43) and erucic acid
(41.0 %) could determine just this species
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