The effect of geographic location on circannual adrenocorticotropic hormone plasma concentrations in horses in Australia

Background: Longitudinal evaluation of plasma endogenous ACTH concentration in clinically normal horses has not been investigated in the Southern Hemisphere. Objectives: To longitudinally determine monthly upper reference limits for plasma ACTH in 2 disparate Australian geographic locations and to examine whether location affected the circannual rhythm of endogenous ACTH in the 2 groups of horses over a 12-month period. Animals: Clinically normal horses <20 years of age from 4 properties (institutional herd and client owned animals) in Perth (n = 40) and Townsville (n = 41) were included in the study. Methods: A prospective longitudinal descriptive study to determine the upper reference limit and confidence intervals for plasma ACTH in each geographic location using the ASVCP reference interval (RI) guidelines, for individual months and monthly groupings for 12 consecutive months. Results: Plasma endogenous ACTH concentrations demonstrated a circannual rhythm. The increase in endogenous ACTH was not confined to the autumnal months but was associated with changes in photoperiod. During the quiescent period, plasma ACTH concentrations were lower, ≤43 pg/mL (upper limit of the 90% confidence interval (CI)) in horses from Perth and ≤67 pg/mL (upper limit of the 90% CI) in horses from Townsville, than at the acrophase, ≤94 pg/mL (upper limit of the 90% CI) in horses from Perth, ≤101 pg/mL (upper limit of the 90% CI) in horses from Townsville. Conclusions and Clinical Importance: Circannual rhythms of endogenous ACTH concentrations vary between geographic locations, this could be due to changes in photoperiod or other unknown factors, and upper reference limits should be determined for specific locations

J Interpers Violence

Homicide-suicide incidents involving child victims can have a detrimental impact on survivors of the violence, family members and friends of the decedents, and other community members, but the rare occurrence of these acts makes using quantitative data to examine their associated antecedents challenging. Therefore, using qualitative data from the 2003-2011 National Violent Death Reporting System, we examined 175 cases of homicide-suicide involving child victims in an effort to better understand the complex situational factors of these events. Our findings indicate that 98% of homicide-suicides with child victims are perpetrated by adults (mostly parents) and propelled by the perpetrators' intimate partner problems, mental health problems, and criminal/legal problems. These events are often premeditated, and plans for the violence are sometimes disclosed prior to its occurrence. Findings provide support for several theoretical perspectives, and implications for prevention are discussed.CC999999/Intramural CDC HHS/United States2019-02-01T00:00:00Z26385898PMC4795978vault:1634

Floor vibration serviceability in a multistory factory building

ArticleExperimental and analytical modal analysis and in-operation vibration measurements were performed on the massive concrete structural floors of several structurally connected ‘units’ of a six-level, multitenant industrial complex with total floor usable area exceeding 0.1 km2. The aim of the systematic study was to characterize vibration sources and factors that affect vibration serviceability, which is a major concern when changing usage patterns lead to conflicting requirements for vibration generation and tolerance for different types of industrial/ commercial user. This was a rare investigation aiming to provide information on specific performance and relevant technologies for occupancy decisions by tenants and building management of similar structures. Floors evaluated were within different types of industrial singleoccupant unit stacked up to six levels and having multibay floors with spans up to 12 m with first vibration mode frequencies greater than 8 Hz. These “high-frequency floors” display typical transient response behavior to footfalls, with response levels controlled by modal mass. Units were studied in typical operational conditions including warehousing, instrument assembly and testing, light electronic/mechanical manufacturing, and machining. Vibration sources included internal and external vehicles, human footfalls, and machinery. The study showed the most onerous form of loading to be forklift trucks and that higher level floors of the same type were least serviceable. Experimental modal analysis showed a surprising range of modal properties for nominally identical floors of the same type and the relevance to performance of modal mass

Resequencing And Nucleotide Variation Of Sucrose Synthase (Nmsusy1) Gene In A Tropical Timber Tree Neolamarckia Macrophylla

Sucrose synthase (SuSy) is a key enzyme that catalyses the reversible synthesis and degradation of sucrose. It provides greater impact in regulating the photosynthetic processes and environmental stresses in plants. Thus, the nucleotide variation of partial NmSusy1 genomic DNAs (750 bp) generated through PCR amplification was examined in this study, and this followed by resequencing from 15 selected Neolamarckia macrophylla clones. The consensus sequences were aligned to detect the presence of single nucleotide polymorphisms (SNPs). In total, five SNPs were detected at nucleotide 1, 2, 34, 35 and 397. Of these, four SNPs were located at the predicted coding region while one SNP was located at the predicted non-coding region. Interestingly, one single base pair InDel polymorphism was also detected at nucleotide 17. On average, one SNP at every 150 bp was detected based on the 15 NmSusy1 sequences. There was one non-synonymous mutation detected, whereby amino acid glutamic acid (E) was replaced by arginine (R) in one of the 15 samples tested. This non-synonymous SNP might change the structural, functional or biochemical properties of the enzyme being produced and therefore possibly lead to changes in phenotypic characteristic of the trees. Overall, this study has demonstrated that resequencing is an effective technique for classifying molecular diversity or nucleotide variation in the Susy gene of N. macrophylla. Those SNPs, once validated, could potentially be used as a tool in marker-assisted selection (MAS) that enables more precise and accurate in the selection and prediction of yield or performance at the early developmental stages, such as at the seedling stage

Bio-economic evaluation and optimization of livestock intensification in the Central Highlands of Vietnam.

Beef cattle have high market demand in Vietnam and the Dak Lak local government encourages the development of beef value chains. Household surveys were carried out in Cu Jut and Ea Kar districts and farming systems and production specialization were found to differ in each district. Ea Kar farmers were more specialized in livestock production while Cu Jut farmers were more focused on cash crop production. The FarmDESIGN bio-economic model allowed us to study two representative farms, one from Ea Kar and one from Cu Jut district. The Ea Kar farm had a more integrated livestock production system, providing manure to the fields that produced feed for the livestock. Both farms had high farm-level nitrogen balances due to high feed and fertilizer imports. The soil organic matter (SOM) balance in Cu Jut was negative (-48 kg/ha) because of its manure management strategy. On both farms, the residues were removed from the fields, providing no input to SOM and were fed to livestock (Ea Kar) or burnt (CuJut). Livestock intensification scenarios that were implemented for the Ea Kar case study farm showed two possible pathways – forage-based and grain-based cattle fattening. Both strategies could lead to higher operating profits (+35% for forage-based cattle fattening and +59% for grain-based cattle fattening) and lower labor demands if they were skillfully implemented for the latter scenario. However, grain-based fattening negatively affected SOM balance, in contrast to forage-based fattening. The optimization of the current Ea Kar farm with FarmDESIGN indicated that there are options to change the farm setup in order to increase profitability and reduce family labor demands. However there are some trade-offs to consider. If reducing environmental impact is a priority, there are alternative farm configurations that will produce lower greenhouse gas emissions while increasing SOM and increasing overall farm profitability. These should be assessed along with the farmers’ interests and priorities. Quantitative farm modeling of complex mixed farming systems can assess potential impact and support decision-making, targeting, prioritization and program design for sustainable intensification of livestock systems

Telomere maintenance and telomerase activity are differentially regulated in asexual and sexual worms

In most sexually reproducing animals, replication and maintenance of telomeres occurs in the germ line and during early development in embryogenesis through the use of telomerase. Somatic cells generally do not maintain telomere sequences, and these cells become senescent in adults as telomeres shorten to a critical length. Some animals reproduce clonally and must therefore require adult somatic mechanisms for maintaining their chromosome ends. Here we study the telomere biology of planarian flatworms with apparently limitless regenerative capacity fueled by a population of highly proliferative adult stem cells. We show that somatic telomere maintenance is different in asexual and sexual animals. Asexual animals maintain telomere length somatically during reproduction by fission or when regeneration is induced by amputation, whereas sexual animals only achieve telomere elongation through sexual reproduction. We demonstrate that this difference is reflected in the expression and alternate splicing of the protein subunit of the telomerase enzyme. Asexual adult planarian stem cells appear to maintain telomere length over evolutionary timescales without passage through a germ-line stage. The adaptations we observe demonstrate indefinite somatic telomerase activity in proliferating stem cells during regeneration or reproduction by fission, and establish planarians as a pertinent model for studying telomere structure, function, and maintenance

Predicting Broccoli Development: I. Development Is Predominantly Determined By Temperature Rather Than Photoperiod

Predictive models of broccoli (Brassica oleracea L. var. italica Plenck) ontogeny will aid farmers who need to forecast changes in crop maturity arising from variable climatic conditions so that their forward marketing arrangements can match their anticipated supply. The objective of this study was to quantify the temperature and photoperiod responses of development in a sub-tropical environment from emergence to floral initiation (EFI), and from floral initiation to harvest maturity (FIHM). Three cultivars, ('Fiesta', 'Greenbelt' and 'Marathon') were sown on eight dates from 11 March to 22 May 1997 and grown under natural and extended (16 h) photoperiods at Gatton College, south-east Queensland, under non-limiting conditions of water and nutrient supply. Climatic data, dates of emergence, floral initiation and harvest maturity were obtained. The estimated base (Tbase) and optimum (Topt) temperatures of 0 and 20 degrees C, respectively were consistent across cultivars, but thermal time requirements were cultivar specific. Differences in thermal time between cultivars during FIHM were small and of little practical importance, but differences in thermal time during EFI were large. Sensitivity to photoperiod and solar radiation was low in the three cultivars used. When the thermal time models were tested on independent data for five cultivars ('Fiesta', 'Greenbelt', 'Marathon', 'CMS Liberty' and 'Triathlon') grown as commercial crops over two years, they adequately predicted floral initiation and harvest maturity

Polariton Analysis of a Four-Level Atom Strongly Coupled to a Cavity Mode

We present a complete analytical solution for a single four-level atom strongly coupled to a cavity field mode and driven by external coherent laser fields. The four-level atomic system consists of a three-level subsystem in an EIT configuration, plus an additional atomic level; this system has been predicted to exhibit a photon blockade effect. The solution is presented in terms of polaritons. An effective Hamiltonian obtained by this procedure is analyzed from the viewpoint of an effective two-level system, and the dynamic Stark splitting of dressed states is discussed. The fluorescence spectrum of light exiting the cavity mode is analyzed and relevant transitions identified.Comment: 12 pages, 9 figure

Vibration serviceability assessment of floors in a multi-use, multi-storey industrial complex

Structures Congress 2014, Boston, Massachusetts, United States, 3 - 5 April 2014Vibration serviceability assessments were carried out on the floors of several 'units' of an industrial complex in Singapore. Floors tested were within large, two-level, structural units described as detached, semi-detached and terraced and having usable single floor areas up to 2100 m2. All floors were constructed from hollow core slabs with spans up to 12 m and all nominally behaved as high frequency floors. Occupancy conditions ranged from empty and untenanted to usage for warehousing, instrument assembly and testing, electronic equipment manufacture to light manufacturing and machining of metal structures. Forms of loading included internal and external vehicles, human footfalls and excitation by machinery. The study identified the most onerous form of loading and provides guidance for facility owners with mixed vibration serviceability requirements

Physiologie appliquee, nutrition et metabolisme

This study examined whether expectancy of ergogenicity of a commonly used nutritional supplement (sodium bicarbonate; NaHCO3) influenced subsequent high-intensity cycling capacity. Eight recreationally active males (age, 21 ± 1 years; body mass, 75 ± 8 kg; height, 178 ± 4 cm; WPEAK = 205 ± 22 W) performed a graded incremental test to assess peak power output (WPEAK), one familiarisation trial and two experimental trials. Experimental trials consisted of cycling at 100% WPEAK to volitional exhaustion (TLIM) 60 min after ingesting either a placebo (PLA: 0.1 g·kg(-1) sodium chloride (NaCl), 4 mL·kg(-1) tap water, and 1 mL·kg(-1) squash) or a sham placebo (SHAM: 0.1 g·kg(-1) NaCl, 4 mL·kg(-1) carbonated water, and 1 mL·kg(-1) squash). SHAM aimed to replicate the previously reported symptoms of gut fullness (GF) and abdominal discomfort (AD) associated with NaHCO3 ingestion. Treatments were administered double blind and accompanied by written scripts designed to remain neutral (PLA) or induce expectancy of ergogenicity (SHAM). After SHAM mean TLIM increased by 9.5% compared to PLA (461 ± 148 s versus 421 ± 150 s; P = 0.048, d = 0.3). Ratings of GF and AD were mild but ~1 unit higher post-ingestion for SHAM. After 3 min TLIM overall ratings of perceived exertion were 1.4 ± 1.3 units lower for SHAM compared to PLA (P = 0.020, d = 0.6). There were no differences between treatments for blood lactate, blood glucose, or heart rate. In summary, ergogenicity after NaHCO3 ingestion may be influenced by expectancy, which mediates perception of effort during subsequent exercise. The observed ergogenicity with SHAM did not affect our measures of cardiorespiratory physiology or metabolic flux.University of Derby Departmental Teaching Budge