70 research outputs found
Association between carrier screening and incidence of cystic fibrosis
Context A downward trend in cystic fibrosis (CF) birth incidence has been reported in some areas.
Objective To evaluate the association between carrier screening and CF birth incidence.
Design, Setting, and Participants In northeastern Italy, CF birth incidence is monitored by means of a long-standing neonatal screening program. In the same area, 2 sections using different carrier detection approaches were identified—the western region, in which CF carrier tests are offered only to relatives of patients or to couples planning in vitro fertilization; and the eastern region, in which carrier testing is offered to relatives and carrier screening to infertile couples and to couples of reproductive age. A total of 779 631 newborns underwent CF neonatal screening between January 1993 and December 2007, of whom 195 had CF detected.
Main Outcome Measure Cystic fibrosis birth incidence in the 2 regions.
Results A time-related decrease in birth incidence was found, with a mean annual percentage decrease of 0.16 per 10 000 neonates (P < .001). In the western region, 2559 carrier tests were performed, 314 carriers were identified, and 9 carrier couples were detected. In the eastern region, 87 025 carrier tests were performed, 3650 carriers were identified, and 82 carrier couples were detected. The birth rate decrease was greater in the eastern region (decrease rate, 0.24; 95% confidence interval [CI], 0.12-0.36) than in the western region (decrease rate, 0.04; 95% CI, –0.16 to 0.08; P = .01). The increase in the number of screened carriers over time was significantly correlated with the decrease in CF birth incidence (correlation coefficient = −0.53; 95% CI, –0.20 to –0.74; P = .003).
Conclusion In northeastern Italy, carrier screening was associated with a decrease in the incidence of CF
EXPERIÊNCIA NA EXTENSÃO UNIVERSITÁRIA: DISFAGIA OROFARÍNGEA.
A disfagia orofaríngea é um sintoma que compromete o ato de deglutição, em qualquer etapa
do trajeto do alimento da boca ao estômago, que ocorre em consequência de alguma doença
ou evento neurológico, mecânico, imunológico, etc. Este trabalho tem o objetivo de
apresentar as ações que foram e serão desenvolvidas no ano de 2019 pelo projeto de extensão
“Disfagia Orofaríngea: eu sei o que é e posso ajudar”, vinculado ao curso de Fonoaudiologia
da UFCSPA a 4 anos. O projeto visa interagir, comunicar e compartilhar conhecimento entre
todos os estudantes e profissionais da área da saúde e da comunidade envolvidos no nosso
trabalho. A nossa principal ação ocorre semanalmente, com os pacientes à beira do leito e
com a equipe multidisciplinar do Hospital Santa Clara, da Irmandade Santa Casa de
Misericórdia de Porto Alegre. Na semana de Atenção à Disfagia, foram desenvolvidas três
ações, são elas: Ação em um parque da cidade de Porto Alegre, onde foram entregues folders
informativos e realizadas orientações e esclarecimentos sobre disfagia; Atividade nas
dependências da UFCSPA, voltada para a comunidade interna e interessados, onde também
foi explicado de forma geral e simples, os principais tópicos sobre a disfagia; Organização
do IV Evento Comemorativo de Atenção à Disfagia, o qual ultrapassou 100 inscrições
prévias. Em maio, participaremos do UFCSPA Acolhe, evento acadêmico que objetiva levar
as atividades desenvolvidas em âmbito universitário para a comunidade. Diante das nossas
ações como projeto de extensão, observamos, muitas vezes, a falta de informação de diversos
públicos, inclusive da área da saúde, acerca da disfagia. Por esta razão, percebemos a
importância da nossa atuação como projeto de extensão que visa a disseminação desse
conhecimento não só nas atividades de extensão, mas também na pesquisa e no ensino do
nosso tema principal para todos indivíduos
Evidence for the Involvement of Lipid Rafts and Plasma Membrane Sphingolipid Hydrolases in Pseudomonas aeruginosa
Cystic fibrosis (CF) is the most common autosomal genetic recessive disease caused by mutations of gene encoding for the cystic fibrosis transmembrane conductance regulator. Patients with CF display a wide spectrum of symptoms, the most severe being chronic lung infection and inflammation, which lead to onset of cystic fibrosis lung disease. Several studies indicate that sphingolipids play a regulatory role in airway inflammation. The inhibition and downregulation of GBA2, the enzyme catabolizing glucosylceramide to ceramide, are associated with a significant reduction of IL-8 production in CF bronchial epithelial cells. Herein, we demonstrate that GBA2 plays a role in the proinflammatory state characterizing CF cells. We also report for the first time that Pseudomonas aeruginosa infection causes a recruitment of plasma membrane-associated glycosphingolipid hydrolases into lipid rafts of CuFi-1-infected cells. This reorganization of cell membrane may be responsible for activation of a signaling cascade, culminating in aberrant inflammatory response in CF bronchial epithelial cells upon bacterial infection. Taken together, the presented data further support the role of sphingolipids and their metabolic enzymes in controlling the inflammatory response in CF
Evidence for the Involvement of Lipid Rafts and Plasma Membrane Sphingolipid Hydrolases in Pseudomonas aeruginosa Infection of Cystic Fibrosis Bronchial Epithelial Cells
Cystic fibrosis (CF) is the most common autosomal genetic recessive disease caused by mutations of gene encoding for the cystic fibrosis transmembrane conductance regulator. Patients with CF display a wide spectrum of symptoms, the most severe being chronic lung infection and inflammation, which lead to onset of cystic fibrosis lung disease. Several studies indicate that sphingolipids play a regulatory role in airway inflammation. The inhibition and downregulation of GBA2, the enzyme catabolizing glucosylceramide to ceramide, are associated with a significant reduction of IL-8 production in CF bronchial epithelial cells. Herein, we demonstrate that GBA2 plays a role in the proinflammatory state characterizing CF cells. We also report for the first time that Pseudomonas aeruginosa infection causes a recruitment of plasma membrane-associated glycosphingolipid hydrolases into lipid rafts of CuFi-1-infected cells. This reorganization of cell membrane may be responsible for activation of a signaling cascade, culminating in aberrant inflammatory response in CF bronchial epithelial cells upon bacterial infection. Taken together, the presented data further support the role of sphingolipids and their metabolic enzymes in controlling the inflammatory response in CF
Molecular mechanism of action of trimethylangelicin derivatives as CFTR modulators
The psoralen-related compound, 4,6,40-trimethylangelicin (TMA) potentiates the cAMP/PKA-dependent activation of WT-CFTR and rescues F508del-CFTR-dependent chloride secretion in both primary and secondary airway cells homozygous for the F508del mutation. We recently demonstrated that TMA, like lumacaftor (VX-809), stabilizes the first membrane-spanning domain (MSD1) and enhances the interface between NBD1 and ICL4 (MSD2). TMA also demonstrated anti-inflammatory properties, via reduction of IL-8 expression, thus making TMA a promising agent for treatment of cystic fibrosis. Unfortunately, TMA was also found to display potential phototoxicity and mutagenicity, despite the fact that photo-reactivity is absent when the compound is not directly irradiated with UVA light. Due to concerns about these toxic effects, new TMA analogs, characterized by identical or better activity profiles and minimized or reduced side effects, were synthesized by modifying specific structural features on the TMA scaffold, thus generating compounds with no mutagenicity and phototoxicity. Among these compounds, we found TMA analogs which maintained the potentiation activity of CFTR in FRT-YFP-G551D cells. Nanomolar concentrations of these analogs significantly rescued F508del CFTR-dependent chloride efflux in FRT-YFP-F508del, HEK-293 and CF bronchial epithelial cells. We then investigated the ability of TMA analogs to enhance the stable expression of varying CFTR truncation mutants in HEK-293 cells, with the aim
of studying the mechanism of their corrector activity. Not surprisingly, MSD1 was the smallest domain stabilized by TMA analogs, as previously observed for TMA. Moreover, we found that TMA analogs were not effective on F508del-CFTR protein which was already stabilized by a second-site mutation at the NBD1-ICL4 interface. Altogether, our findings demonstrate that these TMA analogs mediate correction by modifying MSD1
and indirectly stabilizing the interface between NBD1 and CL4
GM1 as adjuvant of innovative therapies for cystic fibrosis disease
Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein is expressed at the apical plasma membrane (PM) of different epithelial cells. The most common mutation responsible for the onset of cystic fibrosis (CF), F508del, inhibits the biosynthesis and transport of the protein at PM, and also presents gating and stability defects of the membrane anion channel upon its rescue by the use of correctors and potentiators. This prompted a multiple drug strategy for F508delCFTR aimed simultaneously at its rescue, functional potentiation and PM stabilization. Since ganglioside GM1 is involved in the functional stabilization of transmembrane proteins, we investigated its role as an adjuvant to increase the effectiveness of CFTR modulators. According to our results, we found that GM1 resides in the same PM microenvironment as CFTR. In CF cells, the expression of the mutated channel is accompanied by a decrease in the PM GM1 content. Interestingly, by the exogenous administration of GM1, it becomes a component of the PM, reducing the destabilizing effect of the potentiator VX-770 on rescued CFTR protein expression/function and improving its stabilization. This evidence could represent a starting point for developing innovative therapeutic strategies based on the co-administration of GM1, correctors and potentiators, with the aim of improving F508del CFTR function
Promoção de saúde por meios digitais durante a pandemia da Covid-19 em um projeto de extensão em Disfagia
O projeto de extensão “Disfagia Orofaríngea: eu sei o que é e posso ajudar” visa proporcionar aos estudantes da graduação uma formação global e a inserção na atuação clínica. Devido à pandemia e ao cancelamento das práticas, a extensão reinventou-se, aderindo ao uso mais frequente da Internet para a divulgação de conteúdo. Após o estabelecimento de metas para o período de suspensão das atividades, o grupo organizou-se para criação de material virtual compartilhado na internet com os temas de Disfagia e Covid-19. Houve a continuidade das ações extensionistas do projeto de forma virtual com criação de nova rede social, postagens informativas, vídeos ilustrativos, textos científicos entre outros. A utilização da Internet para divulgação de conteúdos mostrou-se eficiente, apresentando muitas visualizações e possibilitando o aumento da visibilidade do projeto por outras regiões do país e até internacionalmente. O próximo passo será nosso primeiro evento virtual
Effect of modulation of protein kinase C on the cAMP-dependent chloride conductance in T84 cells
AbstractThe regulation of chloride conductance was investigated in the T84 human colon carcinoma cell line by the quenching of the fluorescent probe 6-methoxy-N-(3-sulfopropyl)quinolinium. The permeable cAMP analog 8-Br-cAMP (100 μ) and the calcium ionophore ionomycin (1 μM) activate a chloride conductance. A prolonged (4 h) preincubation of cells with phorbol 12-myristate 13-acetate (100 nM) or with the diacylglycerol analog 1-oleoyl-2-acetyl-glycerol (100 μM): (1) down-modulates to almost zero the protein kinase C activity in the membranes; (ii) inhibits the activation of the chloride conductance mediated by 8-Br-cAMP but not by calcium; (iii) reduces the mRNA without changing the expression of the protein product of the cystic fibrosis gene. The data suggest that PKC is essential for the activation of the cAMP-dependt chloride conductance in T84 cells
Alternative splicing of a previously unidentified CFTR exon introduces an in-frame stop codon 5' of the R region
AbstractThe cystic fibrosis transmembrane conductance regulator (CFTR) has been extensively characterized as the carrier of the basic defect in cystic fibrosis. CFTR is part of a growing family of proteins encoded by a single gene, the variant isoforms of which are generated by alternative splicing or RNA editing. We have analyzed the CFTR mRNA in the region of exons 10–11 in T84 cells and detected an alternatively spliced exon (10b) accounting for about 5% of the CFTR mRNA. The exon lOb found in both the human and mice genomes, introduces an inframe stop codon. The resulting mRNA is translated into a truncated CFTR protein, identified in T84 cells by immunoprecipitation with the CFTR-specific monoclonal antibody MATG 1061. The insertion of a differentially spliced exon carrying an inframe stop codon is a novel cellular mechanism for the production of a protein sharing common sequences with another, but having different properties and functions
Observation of gravitational waves from the coalescence of a 2.5−4.5 M⊙ compact object and a neutron star
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