A study of some spaces related to {\}-open sets

The purpose of this paper is to introduce weakly ${}-{R0}$ spaces, ${}-US$ spaces and to obtain their basic properties. It is shown that weakly ${}-{R0}$ and }-US spaces are preserved under pre-${}$-closed injection and pre-{}$-closed bijection respectively

Operation approach to ß-open sets and applications

In this paper, we introduce the concept of an operation $gamma$ on a family of $beta$-open sets denoted by $beta O(X)$ in a topological space $(X,tau)$. Using the operation $gamma$ on $beta O(X)$, we introduce the concept of $beta$-$gamma$-open sets, and investigate the related topological properties. We also introduce the notion of $beta$-$gamma$-$T_{i}$ spaces $(i =0, 1/2, 1, 2)$ and study some topological properties on them. Further, we introduce $beta$-$(gamma, b)$-continuous maps and investigate basic properties. Finally, we investigate a general operation approach to $beta$-closed graphs of mappings

Friedreich ataxia patient tissues exhibit increased 5-hydroxymethylcytosine modification and decreased CTCF binding at the FXN locus

© 2013 Al-Mahdawi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original author and source are credited.This article has been made available through the Brunel Open Access Publishing Fund.Friedreich ataxia (FRDA) is caused by a homozygous GAA repeat expansion mutation within intron 1 of the FXN gene, which induces epigenetic changes and FXN gene silencing. Bisulfite sequencing studies have identified 5-methylcytosine (5 mC) DNA methylation as one of the epigenetic changes that may be involved in this process. However, analysis of samples by bisulfite sequencing is a time-consuming procedure. In addition, it has recently been shown that 5-hydroxymethylcytosine (5 hmC) is also present in mammalian DNA, and bisulfite sequencing cannot distinguish between 5 hmC and 5 mC.The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number 242193/EFACTS (CS), the Wellcome Trust [089757] (SA) and Ataxia UK (RMP) to MAP

Non-catalytic Roles of Tet2 Are Essential to Regulate Hematopoietic Stem and Progenitor Cell Homeostasis

The Ten-eleven translocation (TET) enzymes regulate gene expression by promoting DNA demethylation and partnering with chromatin modifiers. TET2, a member of this family, is frequently mutated in hematological disorders. The contributions of TET2 in hematopoiesis have been attributed to its DNA demethylase activity, and the significance of its nonenzymatic functions has remained undefined. To dissect the catalytic and non-catalytic requirements of Tet2, we engineered catalytically inactive Tet2 mutant mice and conducted comparative analyses of Tet2 mutant and Tet2 knockout animals. Tet2 knockout mice exhibited expansion of hematopoietic stem and progenitor cells (HSPCs) and developed myeloid and lymphoid disorders, while Tet2 mutant mice predominantly developed myeloid malignancies reminiscent of human myelodysplastic syndromes. HSPCs from Tet2 knockout mice exhibited distinct gene expression profiles, including downregulation of Gata2. Overexpression of Gata2 in Tet2 knockout bone marrow cells ameliorated disease phenotypes. Our results reveal the non-catalytic roles of TET2 in HSPC homeostasis

Comparison of sequencing-based methods to profile DNA methylation and identification of monoallelic epigenetic modifications.

Analysis of DNA methylation patterns relies increasingly on sequencing-based profiling methods. The four most frequently used sequencing-based technologies are the bisulfite-based methods MethylC-seq and reduced representation bisulfite sequencing (RRBS), and the enrichment-based techniques methylated DNA immunoprecipitation sequencing (MeDIP-seq) and methylated DNA binding domain sequencing (MBD-seq). We applied all four methods to biological replicates of human embryonic stem cells to assess their genome-wide CpG coverage, resolution, cost, concordance and the influence of CpG density and genomic context. The methylation levels assessed by the two bisulfite methods were concordant (their difference did not exceed a given threshold) for 82% for CpGs and 99% of the non-CpG cytosines. Using binary methylation calls, the two enrichment methods were 99% concordant and regions assessed by all four methods were 97% concordant. We combined MeDIP-seq with methylation-sensitive restriction enzyme (MRE-seq) sequencing for comprehensive methylome coverage at lower cost. This, along with RNA-seq and ChIP-seq of the ES cells enabled us to detect regions with allele-specific epigenetic states, identifying most known imprinted regions and new loci with monoallelic epigenetic marks and monoallelic expression

Voltage Profile improvement by optimal DG allocation using atom search optimisation in radial and mesh distribution system

Recent years have seen a considerable increase in the generation of power using renewable energy sources. This paper deals with the allocation of distributed generations (DG) in radial as well as mesh distribution network (RDSm&MDSm, respectively). The allocation of DG is been done through a meta-heuristic technique known as Atom Search Optimisation. In the paper, the power loss is reduced, along with simultaneous improvement of voltage profile of the system. The proposed algorithm has been tested on IEEE 33 & 69 radial and weakly meshed system with 5 tie lines is considered for power loss minimisation using single and multiple DGs. Two power factors are considered for the simulation 0.8 lag and 0.9 pf lag. The simulation results are carried out in MATLAB

Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Partner TET1

DNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse embryonic stem cells, and hmC levels decrease upon RNA interference–mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC.Chemistry and Chemical Biolog

Defending the genome from the enemy within:mechanisms of retrotransposon suppression in the mouse germline

The viability of any species requires that the genome is kept stable as it is transmitted from generation to generation by the germ cells. One of the challenges to transgenerational genome stability is the potential mutagenic activity of transposable genetic elements, particularly retrotransposons. There are many different types of retrotransposon in mammalian genomes, and these target different points in germline development to amplify and integrate into new genomic locations. Germ cells, and their pluripotent developmental precursors, have evolved a variety of genome defence mechanisms that suppress retrotransposon activity and maintain genome stability across the generations. Here, we review recent advances in understanding how retrotransposon activity is suppressed in the mammalian germline, how genes involved in germline genome defence mechanisms are regulated, and the consequences of mutating these genome defence genes for the developing germline

An Exploratory Study of Confidence in Policing in India

The November 2008 terrorist attack in Mumbai has brought a great deal of attention upon policing in India. In light of the proposed overhauls in policing in India, community policing initiatives have become increasingly utilized across the sub-continent. There remains, however, the important question as to how successful these initiatives can be in a country with such ethnic, class and religious diversity. The study undertaken here is an exploratory examination as to which variables are most closely associated with police confidence. The data for the study drew upon the India Human Development Study 2004-2005 of 41,554 households across India. The results of this study suggest that the variables most significantly associated with confidence in police (human/social capital, religion/caste) are best examined at the state level due to the degree of variance across states. Possible implications for community policing policies in India are also considered