Digital subtraction radiographic analysis of the combination of bioabsorbable membrane and bovine morphogenetic protein pool in human periodontal infrabony defects

Objectives: This study assessed the bone density gain and its relationship with the periodontal clinical parameters in a case series of a regenerative therapy procedure. Material and Methods: Using a split-mouth study design, 10 pairs of infrabony defects from 15 patients were treated with a pool of bovine bone morphogenetic proteins associated with collagen membrane (test sites) or collagen membrane only (control sites). The periodontal healing was clinically and radiographically monitored for six months. Standardized presurgical and 6-month postoperative radiographs were digitized for digital subtraction analysis, which showed relative bone density gain in both groups of 0.034 ± 0.423 and 0.105 ± 0.423 in the test and control group, respectively (p>0.05). Results: As regards the area size of bone density change, the influence of the therapy was detected in 2.5 mm2 in the test group and 2 mm2 in the control group (p>0.05). Additionally, no correlation was observed between the favorable clinical results and the bone density gain measured by digital subtraction radiography (p>0.05). Conclusions: The findings of this study suggest that the clinical benefit of the regenerative therapy observed did not come with significant bone density gains. Long-term evaluation may lead to a different conclusions

Estimulando a Interação de Alunos de Graduação e Pós-graduação na Resolução de Casos Clínicos em Odontologia. Um relato da Aprendizagem e Avaliação.

Aiming to surpass the traditional methods of learning used to teach undergraduate students towards the surgical techniques in Dentistry, it was applied a new pedagogical strategy of learning and evaluation including the Problem Based Learning – PBL and One-Minute Paper concepts. In this task, we enhanced the experience of undergraduate students with different clinical cases and inspired more interactions between graduate and undergraduate students in order to generate a debate seeking the resolution of clinical cases during the last phase of the discipline in the undergraduate program. In this article we present our experience with this new strategy and real testimonials of undergraduate and graduate students.Com o objetivo de superar as formas de ensino tradicionalmente desenvolvidas para a formação dos alunos de graduação em técnicas cirúrgicas odontológicas, desenvolveu-se uma nova prática pedagógica para aprendizagem e prática avaliativa com a incorporação de estratégias do tipo Problem Based Learning – PBL e One-Minute Paper. Nesse processo, ampliamos a exposição do aluno às mais diferentes situações clínicas e estimulamos a interação entre os alunos de graduação e os de pós-graduação para trocarem experiências e discutirem a solução dos casos clínicos durante a última fase da disciplina no curso de graduação. Neste artigo, apresentamos a experiência com essas estratégias, enriquecida com relatos de alunos de graduação e de pós-graduação

Estimulando a Interação de Alunos de Graduação e Pós-graduação na Resolução de Casos Clínicos em Odontologia. Um relato da Aprendizagem e Avaliação.

Com o objetivo de superar as formas de ensino tradicionalmente desenvolvidas para a formação dos alunos de graduação em técnicas cirúrgicas odontológicas, desenvolveu-se uma nova prática pedagógica para aprendizagem e prática avaliativa com a incorporação de estratégias do tipo Problem Based Learning – PBL e One-Minute Paper. Nesse processo, ampliamos a exposição do aluno às mais diferentes situações clínicas e estimulamos a interação entre os alunos de graduação e os de pós-graduação para trocarem experiências e discutirem a solução dos casos clínicos durante a última fase da disciplina no curso de graduação. Neste artigo, apresentamos a experiência com essas estratégias, enriquecida com relatos de alunos de graduação e de pós-graduação.Aiming to surpass the traditional methods of learning used to teach undergraduate students towards the surgical techniques in Dentistry, it was applied a new pedagogical strategy of learning and evaluation including the Problem Based Learning – PBL and One-Minute Paper concepts. In this task, we enhanced the experience of undergraduate students with different clinical cases and inspired more interactions between graduate and undergraduate students in order to generate a debate seeking the resolution of clinical cases during the last phase of the discipline in the undergraduate program. In this article we present our experience with this new strategy and real testimonials of undergraduate and graduate students

The importance of Dentistry in COVID-19 pandemic and the role of saliva as a diagnostic tool

ABSTRACT News of the symptoms caused by a coronavirus in humans began to be documented in December 2019, when several cases of pneumonia caused by the virus occurred in China. Studies are showing the increasing role of saliva and oral cavity in the transmission of the virus, because the receptors of epithelial cells in this region play a critical role by allowing the virus to enter those cells. The standard method to diagnose the coronavirus is based on the collection of samples in the nasopharynx and oropharynx to detect viral RNA. However, this method results in the interaction between health care professionals and patients, which increases risk of transmission; moreover, this collection can be painful for the patient and cause bleeding. As an alternative, diagnosis can be made using saliva, which only requires patients to deposit the saliva sample in a sterile container. Thus, the objective of this literature review is to present the main forms of contagion and to describe the diagnosis tools, emphasizing the risk that dental professionals are exposed to and, therefore, the growing need for better and faster diagnostic methods for coronavirus, especially using saliva. Medline, PubMed, SciELO, and Bireme databases were used for the literature search with the descriptors: Salivary diagnosis, COVID-19, and Contagion. The review explored the scientific race to develop more effective forms of diagnosis, including saliva tests, and prevention methods taking into account time, accessibility, and cost-benefit and, as a consequence, the role of dentistry has been continuously increasing

Antimicrobial photodynamic therapy as an adjunct to non-surgical treatment of aggressive periodontitis: a split-mouth randomized controlled trial

The management of aggressive periodontitis (AgP) represents a challenge for clinicians because there are no standardized protocols for an efficient control of the disease. This randomized controlled clinical trial evaluated the effects of repeated applications of antimicrobial photodynamic therapy (aPDT) adjunctive to scaling and root planing (SRP) in patients with AgP. Using a split-mouth design, 20 patients with generalized AgP were treated with aPDT + SRP (test group) or SRP only (control group). aPDT was applied at four periods. All patients were monitored for 90 days. Clinical, microbiologic, and immunologic parameters were statistically analyzed. In deep periodontal pocket analysis (probing depth [PD] >= 7 mm at baseline), the test group presented a decrease in PD and a clinical attachment gain significantly higher than the control group at 90 days (P <0.05). The test group also demonstrated significantly less periodontal pathogens of red and orange complexes and a lower interleukin-1 beta/interleukin-10 ratio than the control group (P <0.05). The application of four sessions of aPDT, adjunctive to SRP, promotes additional clinical, microbiologic, and immunologic benefits in the treatment of deep periodontal pockets in single-rooted teeth in patients with AgP86337638

Adjunct effect of the antimicrobial photodynamic therapy to an association of non-surgical and surgical periodontal treatment in modulation of gene expression

Background: This study has evaluated the effect of antimicrobial photodynamic therapy (aPDT) used in conjunction with non-surgical and surgical periodontal treatment (PT) in modulating gene expression during periodontal wound healing. Methods: Fifteen patients with chronic periodontitis, presenting bilaterally lower molars with class III furcation lesions and scheduled for extraction, were selected. In initial therapy, scaling and root planing (SRP) was performed in the Control Group (CG), while SRP + aPDT were performed in the Test Group (TG). 45 days later, flap surgery plus SRP, and flap surgery plus SRP + aPDT were performed in the CG and TG, respectively. At 21 days post-surgery, the newly formed granulation tissue was collected, and Real-time PCR evaluated the expression of the genes: tumor necrosis factor-?, interleukin-1?, interleukin-4, interleukin-10, matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2), osteoprotegerin (OPG), receptor activator of nuclear factor- ?B ligand (RANKL), type I collagen, alkaline phosphatase, osteopontin, osteocalcin, and bone sialoprotein. Results: There were statistically significant differences between the groups in relation to mRNA levels for MMP-2 (TG = 3.26 ± 0.89; CG = 4.23 ± 0.97; p = 0.01), TIMP-2/MMP-2 ratio (TG = 0.91 ± 0.34; CG = 0.73 ± 0.32; p = 0.04), OPG (TG = 0.84 ± 0.45; CG = 0.30 ± 0.26; p = 0.001), and OPG/RANKL ratio (TG = 0.60 ± 0.86; CG = 0.23 ± 0.16; p = 0.04), favoring the TG. Conclusion: The present data suggest that the aPDT associated to nonsurgical and surgical periodontal therapy may modulate the extracellular matrix and bone remodeling by up regulating the TIMP- 2/MMP-2 and OPG/RANKL mRNA ratio, but the clinical relevance needs to be evaluated in further studies.FAPESP 07/04916-9FAPESP 07/04916