235 research outputs found
Theory of attosecond delays in laser-assisted photoionization
We study the temporal aspects of laser-assisted extreme ultraviolet (XUV)
photoionization using attosecond pulses of harmonic radiation. The aim of this
paper is to establish the general form of the phase of the relevant transition
amplitudes and to make the connection with the time-delays that have been
recently measured in experiments. We find that the overall phase contains two
distinct types of contributions: one is expressed in terms of the phase-shifts
of the photoelectron continuum wavefunction while the other is linked to
continuum--continuum transitions induced by the infrared (IR) laser probe. Our
formalism applies to both kinds of measurements reported so far, namely the
ones using attosecond pulse trains of XUV harmonics and the others based on the
use of isolated attosecond pulses (streaking). The connection between the
phases and the time-delays is established with the help of finite difference
approximations to the energy derivatives of the phases. This makes clear that
the observed time-delays is a sum of two components: a one-photon Wigner-like
delay and an universal delay that originates from the probing process itself.Comment: 15 pages, 10 figures, special issue 'Attosecond spectroscopy' Chem.
Phy
Two-color ionization of hydrogen by short intense pulses
Photoelectron energy spectra resulting by the interaction of hydrogen with
two short pulses having carrier frequencies, respectively, in the range of the
infrared and XUV regions have been calculated. The effects of the pulse
duration and timing of the X-ray pulse on the photoelectron energy spectra are
discussed. Analysis of the spectra obtained for very long pulses show that
certain features may be explained in terms of quantum interferences in the time
domain. It is found that, depending on the duration of the X-ray pulse, ripples
in the energy spectra separated by the infrared photon energy may appear.
Moreover, the temporal shape of the low frequency radiation field may be
inferred by the breadth of the photoelectron energy spectra.Comment: 12 pages, 8 figure
Probing single-photon ionization on the attosecond time scale
We study photoionization of argon atoms excited by attosecond pulses using an
interferometric measurement technique. We measure the difference in time delays
between electrons emitted from the and from the shell, at
different excitation energies ranging from 32 to 42 eV. The determination of
single photoemission time delays requires to take into account the measurement
process, involving the interaction with a probing infrared field. This
contribution can be estimated using an universal formula and is found to
account for a substantial fraction of the measured delay.Comment: 4 pages, 4 figures, under consideratio
Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of osmotic pressure on single cell cycle dynamics remains poorly understood. Here, we investigate the effect of osmotic pressure on single cell cycle dynamics, nuclear growth, proliferation, migration and protein expression, by quantitative time-lapse imaging of single cells genetically modified with fluorescent ubiquitination-based cell cycle indicator 2 (FUCCI2). Single cell data reveals that under hyperosmotic stress, distinct cell subpopulations emerge with impaired nuclear growth, delayed or growth arrested cell cycle and reduced migration. This state is reversible for mild hyperosmotic stress, where cells return to regular cell cycle dynamics, proliferation and migration. Thus, osmotic pressure can modulate the reversible growth arrest and reactivation of human metastatic cells
In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events
The bone structure is very dynamic and continuously adapts its geometry to external stimuli by modeling and remodeling the mineralized tissue. In vivo microCT-based time-lapse morphometry is a powerful tool to study the temporal and spatial dynamics of bone (re)modeling. Here an advancement in the methodology to detect and quantify site-specific differences in bone (re)modeling of 12-week-old BALB/c nude mice is presented. We describe our method of quantifying new bone surface interface readouts and how these are influenced by bone curvature. This method is then used to compare bone surface (re)modeling in mice across different anatomical regions to demonstrate variations in the rate of change and spatial gradients thereof. Significant differences in bone (re)modeling baseline parameters between the metaphyseal and epiphyseal are shown, as well as cortical and trabecular bone of the distal femur and proximal tibia. These results are validated using conventional static in vivo microCT analysis. Finally, the insights from these new baseline values of physiological bone (re)modeling were used to evaluate pathological bone (re)modeling in a pilot breast cancer bone metastasis model. The method shows the potential to be suitable to detect early pathological events and track their spatio-temporal development in both cortical and trabecular bone. This advancement in (re)modeling surface analysis and defined baseline parameters according to distinct anatomical regions will be valuable to others investigating various disease models with site-distinct local alterations in bone (re)modeling.ER
Experimental study of fragmentation products in the reactions 112Sn + 112Sn and 124Sn + 124Sn at 1 AGeV
Production cross-sections and longitudinal velocity distributions of the
projectile-like residues produced in the reactions 112Sn + 112Sn and 124Sn +
124Sn both at an incident beam energy of 1 AGeV were measured with the
high-resolution magnetic spectrometer, the Fragment Separator (FRS) of GSI. For
both reactions the characteristics of the velocity distributions and nuclide
production cross sections were determined for residues with atomic number Z
10. A comparison of the results of the two reactions is presented.Comment: 14 pages, 12 figure
Resolved-sideband Raman cooling to the ground state of an optical lattice
We trap neutral Cs atoms in a two-dimensional optical lattice and cool them
close to the zero-point of motion by resolved-sideband Raman cooling. Sideband
cooling occurs via transitions between the vibrational manifolds associated
with a pair of magnetic sublevels and the required Raman coupling is provided
by the lattice potential itself. We obtain mean vibrational excitations
\bar{n}_x \approx \bar{n}_y \approx 0.01, corresponding to a population \sim
98% in the vibrational ground state. Atoms in the ground state of an optical
lattice provide a new system in which to explore quantum state control and
subrecoil laser coolingComment: PDF file, 13 pages including 3 figure
Quantum-state control in optical lattices
We study the means to prepare and coherently manipulate atomic wave packets
in optical lattices, with particular emphasis on alkali atoms in the
far-detuned limit. We derive a general, basis independent expression for the
lattice operator, and show that its off-diagonal elements can be tailored to
couple the vibrational manifolds of separate magnetic sublevels. Using these
couplings one can evolve the state of a trapped atom in a quantum coherent
fashion, and prepare pure quantum states by resolved-sideband Raman cooling. We
explore the use of atoms bound in optical lattices to study quantum tunneling
and the generation of macroscopic superposition states in a double-well
potential. Far-off-resonance optical potentials lend themselves particularly
well to reservoir engineering via well controlled fluctuations in the
potential, making the atom/lattice system attractive for the study of
decoherence and the connection between classical and quantum physics.Comment: 35 pages including 8 figures. To appear in Phys. Rev. A. March 199
How to integrate atopic dermatitis in the management of skin neglected tropical diseases in Sub‐Saharan Africa?
Lack of Adiponectin Drives Hyperosteoclastogenesis in Lipoatrophic Mice.
Long bones from mammals host blood cell formation and contain multiple cell types, including adipocytes. Physiological functions of bone marrow adipocytes are poorly documented. Herein, we used adipocyte-deficient PPARγ-whole body null mice to investigate the consequence of total adipocyte deficiency on bone homeostasis in mice. We first highlighted the dual bone phenotype of PPARγ null mice: one the one hand, the increased bone formation and subsequent trabecularization extending in the long bone diaphysis, due to the well-known impact of PPARγ deficiency on osteoblasts formation and activity; on the other hand, an increased osteoclastogenesis in the cortical bone. We then further explored the cause of this unexpected increased osteoclastogenesis using two independent models of lipoatrophy, which recapitulated this phenotype. This demonstrates that hyperosteoclastogenesis is not intrinsically linked to PPARγ deficiency, but is a consequence of the total lipodystrophy. We further showed that adiponectin, a cytokine produced by adipocytes and mesenchymal stromal cells is a potent inhibitor of osteoclastogenesis in vitro and in vivo. Moreover, pharmacological activation of adiponectin receptors by the synthetic agonist AdipoRon inhibited mature osteoclast activity both in mouse and human cells by blocking podosome formation through AMPK activation. Finally, we demonstrated that AdipoRon treatment blocks bone erosion in vivo in a murine model of inflammatory bone loss, providing potential new approaches to treat osteoporosis
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