475 research outputs found
Expression and trans-specific polymorphism of self-incompatibility RNases in Coffea (Rubiaceae)
Self-incompatibility (SI) is widespread in the angiosperms, but identifying the biochemical components of SI mechanisms has proven to be difficult in most lineages. Coffea (coffee; Rubiaceae) is a genus of old-world tropical understory trees in which the vast majority of diploid species utilize a mechanism of gametophytic self-incompatibility (GSI). The S-RNase GSI system was one of the first SI mechanisms to be biochemically characterized, and likely represents the ancestral Eudicot condition as evidenced by its functional characterization in both asterid (Solanaceae, Plantaginaceae) and rosid (Rosaceae) lineages. The S-RNase GSI mechanism employs the activity of class III RNase T2 proteins to terminate the growth of "self" pollen tubes. Here, we investigate the mechanism of Coffea GSI and specifically examine the potential for homology to S-RNase GSI by sequencing class III RNase T2 genes in populations of 14 African and Madagascan Coffea species and the closely related self-compatible species Psilanthus ebracteolatus. Phylogenetic analyses of these sequences aligned to a diverse sample of plant RNase T2 genes show that the Coffea genome contains at least three class III RNase T2 genes. Patterns of tissue-specific gene expression identify one of these RNase T2 genes as the putative Coffea S-RNase gene. We show that populations of SI Coffea are remarkably polymorphic for putative S-RNase alleles, and exhibit a persistent pattern of trans-specific polymorphism characteristic of all S-RNase genes previously isolated from GSI Eudicot lineages. We thus conclude that Coffea GSI is most likely homologous to the classic Eudicot S-RNase system, which was retained since the divergence of the Rubiaceae lineage from an ancient SI Eudicot ancestor, nearly 90 million years ago.United States National Science Foundation [0849186]; Society of Systematic Biologists; American Society of Plant Taxonomists; Duke University Graduate Schoolinfo:eu-repo/semantics/publishedVersio
Lactate signalling regulates fungal β-glucan masking and immune evasion
AJPB: This work was supported by the European Research Council (STRIFE, ERC- 2009-AdG-249793), The UK Medical Research Council (MR/M026663/1), the UK Biotechnology and Biological Research Council (BB/K017365/1), the Wellcome Trust (080088; 097377). ERB: This work was supported by the UK Biotechnology and Biological Research Council (BB/M014525/1). GMA: Supported by the CNPq-Brazil (Science without Borders fellowship 202976/2014-9). GDB: Wellcome Trust (102705). CAM: This work was supported by the UK Medical Research Council (G0400284). DMM: This work was supported by UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC/K000306/1). NARG/JW: Wellcome Trust (086827, 075470,101873) and Wellcome Trust Strategic Award in Medical Mycology and Fungal Immunology (097377). ALL: This work was supported by the MRC Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1).Peer reviewedPostprin
Three-dimensional and two-dimensional echocardiography and biochemical analysis in patients with ST-segment elevation myocardial infarction percutaneously treated: relationship between LV function, remodeling and serum cardiac markers
Basin-wide variations in Amazon forest nitrogen-cycling characteristics as inferred from plant and soil ¹⁵N:¹⁴N measurements
Background: Patterns in tropical forest nitrogen cycling are poorly understood. In particular, the extent to which leguminous trees in these forests fix nitrogen is unclear.
Aims: We aimed to determine factors that explain variation in foliar δ¹⁵N (δ¹⁵NF) for Amazon forest trees, and to evaluate the extent to which putatively N₂-fixing Fabaceae acquire nitrogen from the atmosphere.
Methods: Upper-canopy δ¹⁵NF values were determined for 1255 trees sampled across 65 Amazon forest plots. Along with plot inventory data, differences in δ¹⁵NF between nodule-forming Fabaceae and other trees were used to estimate the extent of N² fixation.
Results: δ¹⁵NF ranged from −12.1‰ to +9.3‰. Most of this variation was attributable to site-specific conditions, with extractable soil phosphorus and dry-season precipitation having strong influences, suggesting a restricted availability of nitrogen on both young and old soils and/or at low precipitation. Fabaceae constituted fewer than 10% of the sampled trees, and only 36% were expressed fixers. We estimated an average Amazon forest symbiotic fixation rate of 3 kg N ha¯¹ year‾¹.
Conclusion: Plant δ¹⁵N indicate that low levels of nitrogen availability are only likely to influence Amazon forest function on immature or old weathered soils and/or where dry-season precipitation is low. Most Fabaceae species that are capable of nodulating do not fix nitrogen in Amazonia
Acidente com material perfurocortante entre profissionais de enfermagem de um hospital universitário
Os riscos ocupacionais a que os profissionais da equipe de enfermagem estão sujeitos no desempenho de suasfunções são consideráveis. Assim estabeleceu-se como objetivo deste estudo analisar os acidentes perfurocortantesno período de 2002 a 2006, envolvendo a equipe de enfermagem de um hospital universitário, para compreender ocontexto em que ocorrem. A utilização destas informações pode ser ferramenta de prevenção. Estudo descritivo,retrospectivo quantitativo e qualitativo. Na análise quantitativa foi utilizada estatística descritiva e na qualitativao discurso do sujeito coletivo. Por meio das fichas de notificação do Núcleo de Vigilância Epidemiológica doHospital, foi possível identificar acidentes do gênero no período pesquisado. Entrevistas foram direcionadas àsvítimas de acidentes que tiveram como paciente-fonte portador de Síndrome da Imunodeficiência Adquirida/Vírus da Imunodeficiência Humana, hepatite B e C. Os achados demonstraram que ainda persiste um grau significativodo desconhecimento ou banalização dos acidentes entre profissionais da saúde
Vasculitic neuropathy presenting as Churg-Strauss paraneoplastic syndrome: a rare association
Small calcified lesions suggestive of neurocysticercosis are associated with mesial temporal sclerosis
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