240 research outputs found
Randomised trials of 6Â % tetrastarch (hydroxyethyl starch 130/0.4 or 0.42) for severe sepsis reporting mortality: systematic review and meta-analysis.
Ethyl pyruvate reduces mortality in an endotoxin-induced severe acute lung injury mouse model
<p>Abstract</p> <p>Background</p> <p>Ethyl pyruvate (EP) was recently identified as an experimental therapeutic agent in a wide variety of model systems for inflammation-mediated tissue and cellular injury.</p> <p>Objective</p> <p>To evaluate the effect of ethyl EP on improving the survival in mice with LPS-induced acute lung injury (ALI).</p> <p>Methods</p> <p>ALI was induced by administering lipopolysaccharide (LPS) intratracheally. The mice were treated intraperitoneally (i.p.) with 100, 50 and 10 mg/kg EP immediately before intratracheal instillation of LPS, and 100 mg/kg EP was administered 0, 12, 24 and 48 hours after induction of ALI. The mortality rate was recorded and analyzed by the Kaplan-Meier method. Serum tumor necrosis factor (TNF)-α, interleukin (IL) -6 and IL-1 ÎČ were measured in bronchial alveolar lavage fluid using an enzyme-linked immunosorbent assay. High-mobility group box 1 levels were measured by Western immunoblotting.</p> <p>Results</p> <p>Treatment with EP significantly inhibited the release of HMGB1, TNF-α, IL-6 and IL-1ÎČ into bronchoalveolar lavage (BAL) fluids of ALI mice, and reduced the permeability index of the injured lung. High EP doses reduced the mortality from ALI and the permeability index (100 mg/kg and 50 mg/kg EP versus control; P < 0.0001). Early administration of high-dose EP significantly increased survival rate (0, 12 and 24 h versus control; P < 0.0001, P < 0.0001 and P = 0.01 respectively by log-rank test). There was no survival advantage when EP was initiated at 48 h.</p> <p>Conclusion</p> <p>Ethyl pyruvate improves survival and reduces the lung permeability index in mice with LPS-induced ALI.</p
Assessing Attachment to Parents and Peers in Middle Childhood: Psychometric Studies of the Portuguese Version of the People in My Life Questionnaire
Common HLA Alleles Associated with Health, but Not with Facial Attractiveness
Three adaptive hypotheses have been proposed to explain the link between the human leucocyte antigen (HLA) genes, health measures and facial attractiveness: inbreeding avoidance, heterozygote advantage and frequency-dependent selection. This paper reports findings that support a new hypothesis relating HLA to health. We suggest a new method to quantify the level of heterozygosity. HLA heterozygosity did not significantly predict health measures in women, but allele frequency did. Women with more common HLA alleles reported fewer cold and flu bouts per year, fewer illnesses in the previous year and rated themselves healthier than women with rare alleles. To our knowledge, this is the first study to show a positive correlation between HLA allele frequency and general health measures. We propose that certain common HLA alleles confer resistance to prevalent pathogens. Nevertheless, neither HLA heterozygosity nor allele frequency significantly predicted how healthy or attractive men rated the female volunteers. Three non-mutually exclusive explanations are put forward to explain this finding
Inflexible Youngsters: Psychological and Psychopathological Correlates of the Avoidance and Fusion Questionnaire for Youths in Nonclinical Dutch Adolescents
The Contribution of Occult Precipitation to Nutrient Deposition on the West Coast of South Africa
The Strandveld mediterranean-ecosystem of the west coast of South Africa supports floristically
diverse vegetation growing on mostly nutrient-poor aeolian sands and extending from
the Atlantic Ocean tens of kilometers inland. The cold Benguela current upwelling interacts
with warm onshore southerly winds in summer causing coastal fogs in this region. We hypothesized
that fog and other forms of occult precipitation contribute moisture and nutrients
to the vegetation. We measured occult precipitation over one year along a transect running
inland in the direction of the prevailing wind and compared the nutrient concentrations with
those in rainwater. Occult deposition rates of P, N, K, Mg, Ca, Na, Al and Fe all decreased
with distance from the ocean. Furthermore, ratios of cations to Na were similar to those of
seawater, suggesting a marine origin for these. In contrast, N and P ratios in occult precipitation
were higher than in seawater. We speculate that this is due to marine foam contributing
to occult precipitation. Nutrient loss in leaf litter from dominant shrub species was
measured to indicate nutrient demand. We estimated that occult precipitation could meet
the demand of the dominant shrubby species for annual N, P, K and Ca. Of these species,
those with small leaves intercepted more moisture and nutrients than those with larger
leaves and could take up foliar deposits of glycine, NO3-, NH4
+ and Li (as tracer for K)
through leaf surfaces. We conclude that occult deposition together with rainfall deposition
are potentially important nutrient and moisture sources for the Strandveld vegetation that
contribute to this vegetation being floristically distinct from neighbouring nutrient-poor Fynbos
vegetation
Alveolar Epithelial Type II Cells Activate Alveolar Macrophages and Mitigate P. Aeruginosa Infection
Although alveolar epithelial type II cells (AECII) perform substantial roles in the maintenance of alveolar integrity, the extent of their contributions to immune defense is poorly understood. Here, we demonstrate that AECII activates alveolar macrophages (AM) functions, such as phagocytosis using a conditioned medium from AECII infected by P. aeruginosa. AECII-derived chemokine MCP-1, a monocyte chemoattractant protein, was identified as a main factor in enhancing AM function. We proposed that the enhanced immune potency of AECII may play a critical role in alleviation of bacterial propagation and pneumonia. The ability of phagocytosis and superoxide release by AM was reduced by MCP-1 neutralizing antibodies. Furthermore, MCP-1â/â mice showed an increased bacterial burden under PAO1 and PAK infection vs. wt littermates. AM from MCP-1â/â mice also demonstrated less superoxide and impaired phagocytosis over the controls. In addition, AECII conditioned medium increased the host defense of airway in MCP-1â/â mice through the activation of AM function. Mechanistically, we found that Lyn mediated NFÎșB activation led to increased gene expression and secretion of MCP-1. Consequently Lynâ/â mice had reduced MCP-1 secretion and resulted in a decrease in superoxide and phagocytosis by AM. Collectively, our data indicate that AECII may serve as an immune booster for fighting bacterial infections, particularly in severe immunocompromised conditions
Habitat use at fine spatial scale: how does patch clustering criteria explain the use of meadows by red deer ?
Large mammalian herbivores are keystone species
in different ecosystems. To mediate the effects of large
mammalian herbivores on ecosystems, it is crucial to understand
their habitat selection pattern. At finer scales, herbivore
patch selection depends strongly on plant community
traits and therefore its understanding is constrained by patch
definition criteria. Our aim was to assess which criteria for
patch definition best explained use of meadows by wild,
free-ranging, red deer (Cervus elaphus) in a study area in
Northeast Portugal. We used two clustering criteria types
based on floristic composition and gross forage classes, respectively.
For the floristic criteria, phytosociological approach
was used to classify plant communities, and its
objectivity evaluated with a mathematical clustering of the
floristic relevés. Cover of dominant plant species was tested
as a proxy for the phytosociological method. For the gross
forage classes, the graminoids/forbs ratio and the percentage
cover of legumes were used. For assessing deer relative use of
meadows we used faecal accumulation rates. Patches clustered
according to floristic classification better explained selection of patches by deer. Plant community classifications based on
phytosociology, or proxies of this, used for characterizing
meadow patches resulted useful to understand herbivore selection
pattern at fine scales and thus potentially suitable to
assist wildlife management decisions
The impact of sex-role reversal on the diversity of the major histocompatibility complex: Insights from the seahorse (Hippocampus abdominalis)
Background: Both natural and sexual selection are thought to influence genetic diversity, but the study of the relative importance of these two factors on ecologically-relevant traits has traditionally focused on species with conventional sex-roles, with male-male competition and female-based mate choice. With its high variability and significance in both immune function and olfactory-mediated mate choice, the major histocompatibility complex(MHC/MH) is an ideal system in which to evaluate the relative contributions of these two selective forces to genetic diversity. Intrasexual competition and mate choice are both reversed in sex-role reversed species, and sexrelated differences in the detection and use of MH-odor cues are expected to influence the intensity of sexual selection in such species. The seahorse, Hippocampus abdominalis, has an exceptionally highly developed form of male parental care, with female-female competition and male mate choice. Results: Here, we demonstrate that the sex-role reversed seahorse has a single MH class II beta-chain gene and that the diversity of the seahorse MHIIb locus and its pattern of variation are comparable to those detected in species with conventional sex roles. Despite the presence of only a single gene copy, intralocus MHIIb allelic diversity in this species exceeds that observed in species with multiple copies of this locus. The MHIIb locus of the seahorse exhibits a novel expression domain in the male brood pouch. Conclusions: The high variation found at the seahorse MHIIb gene indicates that sex-role reversed species are capable of maintaining the high MHC diversity typical in most vertebrates. Whether such species have evolved the capacity to use MH-odor cues during mate choice is presently being investigated using mate choice experiments. If this possibility can be rejected, such systems would offer an exceptional opportunity to study the effects of natural selection in isolation, providing powerful comparative models for understanding the relative importance of selective factors in shaping patterns of genetic variation
Early Stage Biomineralization in the Periostracum of the âLiving Fossilâ Bivalve Neotrigonia
A detailed investigation of the shell formation of the palaeoheterodont âliving fossilâ Neotrigonia concentrated on the timing and manufacture of the calcified âbossesâ which stud the outside of all trigonioid bivalves (extant and fossil) has been conducted. Electron microscopy and optical microscopy revealed that Neotrigonia spp. have a spiral-shaped periostracal groove. The periostracum itself is secreted by the basal cell, as a thin dark pellicle, becoming progressively transformed into a thin dark layer by additions of secretions from the internal outer mantle fold. Later, intense secretion of the internal surface of the outer mantle fold forms a translucent layer, which becomes transformed by tanning into a dark layer. The initiation of calcified bosses occurred at a very early stage of periostracum formation, deep within the periostracal groove immediately below the initialmost dark layer. At this stage, they consist of a series of polycyclically twinned crystals. The bosses grow as the periostracum traverse through the periostracal groove, in coordination with the thickening of the dark periostracal layer and until, upon reaching the mantle edge, they impinge upon each other and become transformed into large prisms separated by dark periostracal walls. In conclusion, the initial bosses and the external part of the prismatic layer are fully intraperiostracal. With later growth, the prisms transform into fibrous aggregates, although the details of the process are unknown. This reinforces the relationships with other groups that have the ability to form intraperiostracal calcifications, for example the unionoids with which the trigonioids form the clade Paleoheterodonta. The presence of similar structures in anomalodesmatans and other euheterodonts raises the question of whether this indicates a relationship or represents a convergence. The identification of very early calcification within an organic sheet has interesting implications for our understanding of how shells may have evolved.Coordinated Research Projects CGL2010-20748-C02-01 (AGC, EMH) and 02 (CS) (DGI, Spanish MICINN); the Research Group RNM363 (ConsejerĂa de EconomĂa, InvestigaciĂłn, Ciencia y Empleo, Junta de AndalucĂa); and the FP7 COST Action TD0903 of the European Community
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