Effects of Changing Climate and Water Availability on Four Riparian Species in the Southwestern United States

The Lower Colorado River and Rio Grande Basins are home to many riparian species of different degrees of rarity. In our study we focused on two species of birds and two species of gartersnakes that are associated with water: the Yellow-Breasted Chat (Icteria virens), the American Yellow Warbler (Setophaga petechia), the Mexican Gartersnake (Thamnophis eques) and the Narrow-headed Gartersnake (T. rufipunctatus). While the extent of distributions of these species is relatively large, they are often patchily distributed in populations that are small. To evaluate the vulnerability of these species at a landscape scale due to changes in hydrology, we built models of suitability of current and future landscapes for these species. We relied on climatic and hydrological predictions, developed by the Bureau of Reclamation (as part of the WATERSmart program) and NASA’s Moderate-Resolution Imaging Spectroradiometer (MODIS), to derive spatially-explicit metrics that quantify the greenness of riparian vegetation in time. Using WATERSmart data, we were able to project these metrics into the future. The projected changes in water availability by end of the century will directly affect the availability of permanent water and riparian vegetation that surrounds suitable habitats of our study species. Our results suggest significant changes in future landscape suitability (up to 64% of area) for all species that are in addition to other threats. Best models included riparian vegetation as an important component of the predictions but we note that finer scale examination of hydrology and climate effects on habitats would be more useful for effective management

Czech Students’ Perceptions of Ethical Behavior in Business and Entrepreneurship

The central notion of business culture and communication with stakeholders consists in the ethical standards regarding the behaviour of corporation owners and managers, which, on the one hand, form an essential element of successful business activities and partnerships, and on the other hand, are complicated in terms of measuring and managing the phenomenon. This study uses agency and stakeholder theories to compare the ethical standards in the behaviour of businessmen and managers. These theories influence freedom of decision-making and behaviour in entrepreneurial activity based on the personal ethical considerations and individuals’ interests, regardless of the size of their share in the business

Recovery of ectomycorrhizal fungus communities fifteen years after fuels reduction treatments in ponderosa pine forests of the Blue Mountains, Oregon

Managers use restorative fire and thinning for ecological benefits and to convert fuel-heavy forests to fuel-lean landscapes that lessen the threat of stand-replacing wildfire. In this study, we evaluated the long-term impact of thinning and prescribed fire on soil biochemistry and the mycorrhizal fungi associated with ponderosa pine (Pinus ponderosa). Study sites were located in the Blue Mountains of northeastern Oregon where prescribed fire treatments implemented in 1998 and thinning treatments in 2000 included prescribed fire, mechanical thinning of forested areas, a combination of thinning followed by fire, and an untreated control. Soil sampling for this study occurred in 2014 and included four replications of each treatment for a total of 16 experimental units. Differences among treatments in Bray-P, total C and N, and pH were likely driven by the thinning treatments and the resultant deposition of residual slash following harvesting or the consumption of slash by prescribed fire. Similar litter depths across treatments suggest that litter depth stabilizes over time in these forests. After more than a decade of recovery, mycorrhizal fungi in dry inland forests dominated by ponderosa pine that were subjected to fire returned to levels similar to the untreated controls. The results of this study demonstrate the resiliency of these forests to disturbances associated with restoration treatments, providing managers increased flexibility if maintaining abundant and persistent fungal communities for healthy soils is an objective

TGF-beta(2)- and H2O2-Induced Biological Changes in Optic Nerve Head Astrocytes Are Reduced by the Antioxidant Alpha-Lipoic Acid

Background/Aims: The goal of the present study was to determine whether transforming growth factor-beta(2) (TGF-beta(2))- and oxidative stress-induced cellular changes in cultured human optic nerve head (ONH) astrocytes could be reduced by pretreatment with the antioxidant alpha-lipoic acid (LA). Methods: Cultured ONH astrocytes were treated with 1.0 ng/ml TGF-beta(2) for 24 h or 200 mu M hydrogen peroxide (H2O2) for 1 h. Lipid peroxidation was measured by a decrease in cis-pari-naric acid fluorescence. Additionally, cells were pretreated with different concentrations of LA before TGF-beta 2 or H2O2 exposure. Expressions of the heat shock protein (Hsp) alpha B-crystallin and Hsp27, the extracellular matrix (ECM) component fibronectin and the ECM-modulating protein connective tissue growth factor (CTGF) were examined with immunohistochemistry and real-time PCR analysis. Results: Both TGF-beta(2) and H2O2 increased lipid peroxidation. Treatment of astrocytes with TGF-beta(2) and H2O2 upregulated the expression of alpha B-crystallin, Hsp27, fibronectin and CTGF. Pretreatment with different concentrations of LA reduced the TGF-beta(2)- and H2O2-stimulated gene expressions. Conclusion: We showed that TGF-beta(2)- and H2O2-stimulated gene expressions could be prevented by pretreatment with the antioxidant LA in cultured human ONH astrocytes. Therefore, it is tempting to speculate that the use of antioxidants could have protective effects in glaucomatous optic neuropathy. Copyright (C) 2012 S. Karger AG, Base

Structure-guided microbial targeting of antistaphylococcal prodrugs

Carboxy ester prodrugs are widely employed to increase oral absorption and potency of phosphonate antibiotics. Prodrugging can mask problematic chemical features that prevent cellular uptake and may enable tissue-specific compound delivery. However, many carboxy ester promoieties are rapidly hydrolyzed by serum esterases, limiting their therapeutic potential. While carboxy ester-based prodrug targeting is feasible, it has seen limited use in microbes as microbial esterase-specific promoieties have not been described. Here we identify the bacterial esterases, GloB and FrmB, that activate carboxy ester prodrugs i

of Education or Offices within it. NCEO Core Staff

assessment: Results of a comparative study (Technical Report 64). Minneapolis, MN: University of Minnesota, National Center on Educationa

The Role of Thioredoxin Reductases in Brain Development

The thioredoxin-dependent system is an essential regulator of cellular redox balance. Since oxidative stress has been linked with neurodegenerative disease, we studied the roles of thioredoxin reductases in brain using mice with nervous system (NS)-specific deletion of cytosolic (Txnrd1) and mitochondrial (Txnrd2) thioredoxin reductase. While NS-specific Txnrd2 null mice develop normally, mice lacking Txnrd1 in the NS were significantly smaller and displayed ataxia and tremor. A striking patterned cerebellar hypoplasia was observed. Proliferation of the external granular layer (EGL) was strongly reduced and fissure formation and laminar organisation of the cerebellar cortex was impaired in the rostral portion of the cerebellum. Purkinje cells were ectopically located and their dendrites stunted. The Bergmann glial network was disorganized and showed a pronounced reduction in fiber strength. Cerebellar hypoplasia did not result from increased apoptosis, but from decreased proliferation of granule cell precursors within the EGL. Of note, neuron-specific inactivation of Txnrd1 did not result in cerebellar hypoplasia, suggesting a vital role for Txnrd1 in Bergmann glia or neuronal precursor cells

Astrocytes are important mediators of Aβ-induced neurotoxicity and tau phosphorylation in primary culture

Alzheimer's disease (AD) is pathologically characterised by the age-dependent deposition of β-amyloid (Aβ) in senile plaques, intraneuronal accumulation of tau as neurofibrillary tangles, synaptic dysfunction and neuronal death. Neuroinflammation, typified by the accumulation of activated microglia and reactive astrocytes, is believed to modulate the development and/or progression of AD. We have used primary rat neuronal, astrocytic and mixed cortical cultures to investigate the contribution of astrocyte-mediated inflammatory responses during Aβ-induced neuronal loss. We report that the presence of small numbers of astrocytes exacerbate Aβ-induced neuronal death, caspase-3 activation and the production of caspase-3-cleaved tau. Furthermore, we show that astrocytes are essential for the Aβ-induced tau phosphorylation observed in primary neurons. The release of soluble inflammatory factor(s) from astrocytes accompanies these events, and inhibition of astrocyte activation with the anti-inflammatory agent, minocycline, reduces astrocytic inflammatory responses and the associated neuronal loss. Aβ-induced increases in caspase-3 activation and the production of caspase-3-truncated tau species in neurons were reduced when the astrocytic response was attenuated with minocycline. Taken together, these results show that astrocytes are important mediators of the neurotoxic events downstream of elevated Aβ in models of AD, and suggest that mechanisms underlying pro-inflammatory cytokine release might be an important target for therapy

Combinatorial Guidance by CCR7 Ligands for T Lymphocytes Migration in Co-Existing Chemokine Fields

Chemokines mediate the trafficking and positioning of lymphocytes in lymphoid tissues that is crucial for immune surveillance and immune responses. In particular, a CCR7 ligand, CCL21, plays important roles in recruiting T cells to secondary lymphoid tissues (SLT). Furthermore, CCL21 together with another CCR7 ligand, CCL19, direct the navigation and compartmentation of T cells within SLT. However, the distinct roles of these two chemokines for regulating cell trafficking and positioning are not clear. In this study, we explore the effect of co-existing CCL19 and CCL21 concentration fields on guiding T cell migration. Using microfluidic devices that can configure single and superimposed chemokine fields we show that under physiological gradient conditions, human peripheral blood T cells chemotax to CCL21 but not CCL19. Furthermore, T cells migrate away from the CCL19 gradient in a uniform background of CCL21. This repulsive migratory response is predicted by mathematical modeling based on the competition of CCL19 and CCL21 for CCR7 signaling and the differential ability of the two chemokines for desensitizing CCR7. These results suggest a new combinatorial guiding mechanism by CCL19 and CCL21 for the migration and trafficking of CCR7 expressing leukocytes