Use of lipids of Chlorella microalgae in poultry meat marinades and sauces recipes

ArticleThe aim of this study is to develop formulations and technologies for fermented poultry meat products with the addition of whey and lipid extracts obtained from Chlorella microalgae. Lyophilized microalgal biomass was obtained from cell suspensions of Chlorella sorokiniana (strain 211-8k) cultivated in a closed photobioreactor under laboratory conditions. For the cell wall disintegration, the biomass samples were homogenized using a high-speed homogenizer at 10,000 vol min-1 for 5 minutes. The lipid extraction was performed on a Sohxlet apparatus Buchi E-812 SOX with the solvent extraction system ethanol: n-hexane (1: 9). The higher fatty acids composition of the obtained microalgal lipid extracts was determined by gas chromatography with flame-ionization detection using nitrogen as a carrier gas. The ω-3 and ω-6 content represented 26.59% and 19.05% respectively, which indicates that these lipid extracts have high nutritional values. The curd whey was obtained from cow's milk of summer and winter production from 2017 to 2018 (Lomonosov district auxiliary farm, Leningrad region); and lyophilized Direct Vat Set (DVS) cultures (Ch. Hansen, Denmark). The organic acids and carbohydrate content in the serum was determined by ion-exclusion HPLC. The FD-DVS CHN-19 culture was selected to produce a serum with improved organoleptic characteristics and a lower propionic acid content (0.01 g L -1 ). To obtain an optimal ω-3 / ω-6 ratio, a phyto-additive mixture based on sunflower oil and lipid extracts from C. sorokiniana microalgae at a ratio of 5–10: 1 is proposed to be used in recipes and technologies of sauces and marinades. It is established that the use of curd whey marinades allow to increase the water-holding capacity (WHC) by 6−8% and to reduce losses during heat treatment of poultry meat from 2 to 11%

A method for obtaining plastid pigments from the biomass of Chlorella microalgae

Microalgae are distinguished from land plants by the high content of plastid pigments and the biodiversity of carotenoids. The aim of this study is to develop a technology for extracting a pigment complex from the biomass of the microalgae of the genus Chlorella and to determine the extracted pigments’ composition. To obtain biomass, a crude cell suspension of microalgae was used, which was obtained under laboratory conditions for pre-culture cultivation of C. sorokiniana (strain 211-8k). The extraction of plastid pigments from air-dry biomass after disintegration of cell membrane was performed in the 40 kHz mode. It was found that the highest pigment content in ethanol extracts was observed after 30 min (870.0 ± 27.1 mg L -1 ) at 45−50 °C. The pigments’ composition in the resulting total extracts was determined by spectrophotometry and the Reverse Phase HPLC method. The established content of chlorophyll a in the obtained extracts was 537.5 ± 10.0 mg L -1 , the content of chlorophyll b was 182.5 ± 27.5 mg L -1 ; the maximum output of the amount of carotenoids in extracts was 150.0 ± 10.0 mg L -1 . Thus, the main identified forms of carotenoids in extracts from the biomass of microalgae C. sorokiniana were xanthophylls: lutein and fucoxanthin (18.6 and 4.7% of the amount of pigment in extract, respectively) and β-carotene (1.8% of the amount of pigment). It is planned to further fractionate the obtained total extracts of the pigment complex to obtain various forms of chlorophylls and carotenoids to study the spectrum of physiological activity of plastid pigments

Optical studies of the X-ray transient XTE J2123-058 -I. Photometry

We present optical photometry of the X-ray transient XTE J2123-058, obtained in July-October 1998. The light curves are strongly modulated on the 5.95hrs orbital period, and exhibit dramatic changes in amplitude and form during the decline. We used synthetic models which include the effect of partial eclipses and X-ray heating effects, to estimate the system parameters, and we constrain the binary inclination to be i=73+-4 degrees. The model is successful in reproducing the light curves at different stages of the decay by requiring the accretion disc to become smaller and thinner by 30% as the system fades by 1.7 mags in the optical. From Aug 26 the system reaches quiescence with a mean magnitude of R=21.7+-0.1 and our data are consistent with the optical variability being dominated by the companion's ellipsoidal modulation.Comment: 6 pages, 6 figure

THE EFFECT OF HOST PLANT REPLACEMENT ON THE GENETIC STRUCTURE OF THE KRASNODAR GREENBUG <i> SCHIZAPHIS GRAMINUM </i> RONDANI (HOMOPTERA, APHIDIDAE) POPULATION

Characteristics of the new malting spring barley cultivar ‘Omsky 100’ are presented. This cultivar, bred at the Siberian Research As a result of studying the Krasnodar Schizaphis graminum population, a high level of the insect’s overall and seasonal variability in its virulence to sorghum resistance genes was detected. The greenbug population feeding on sorghum was shown to be labile in virulence to barley accessions as well. The selection of S. graminum genotypes specifically adapted to host plant species was observed. During the reproduction of the insect on barley plants, the individuals without “redundant” genes of virulence to sorghum had an advantage in competition. Replacement of the host led to rapid accumulation of clones virulent to the sorghum resistance genes Sgr1 - Sgr4 and Sgr12

Clip thinking impact on the change of educational techniligies in high school

The article views the necessity to change educational technologies in the high school due to such peculiarity of contemporary generation as the clip or mosaic thinking. The author reflects the approaches to the understanding of the notion of clip thinking and offers the ways to improve the educational process taking into account specific features in the youth’s way of thinkingСтатья посвящена рассмотрению вопроса о необходимости изменения образовательных технологий и подходов к обучению студентов высшей школы в связи с формированием у них такой особенности, как клиповое мышление. Автором раскрываются подходы к пониманию клипового мышления как явления, а также предлагаются пути совершенствования образовательного процесса в связи с выявленными особенностями мышления молодеж

On a class of 2D integrable lattice equations

We develop a new approach to the classification of integrable equations of the form $$u_{xy}=f(u, u_x, u_y, \triangle_z u \triangle_{\bar z}u, \triangle_{z\bar z}u),$$ where $\triangle_{ z}$ and $\triangle_{\bar z}$ are the forward/backward discrete derivatives. The following 2-step classification procedure is proposed: (1) First we require that the dispersionless limit of the equation is integrable, that is, its characteristic variety defines a conformal structure which is Einstein-Weyl on every solution. (2) Secondly, to the candidate equations selected at the previous step we apply the test of Darboux integrability of reductions obtained by imposing suitable cut-off conditions

Effects of spin polarization on resonant photoemission from d-f states in TbNi2Mnx compounds

Resonant photoemission in narrow-band materials is described by the sum of first-and secondorder transitions, their quantum-mechanical interference leads to an increase in the spectrum from the valence bands and the appearance of an asymmetric dependence on the photon energy. These effects are studied theoretically and experimentally using the example of three-component intermetallic compounds TbNi2Mnx. The competition between the elastic and inelastic photoemission channels leads to a different dependence of photoemission spectra from nickel and manganese on photon energy. The elastic channel is realized on atoms with large magnetic moments, the inelastic Auger decay occurs on atoms with small moments. © 2018 The Authors, published by EDP Sciences.The research was carried out within the state assignment of FASO of Russia (theme uQ" ant" No. 01201463332), supported in part by RFBR (No. 18-02-00060) and UD RAS (No.15 -8 2-10). The authors express their gratitude to A. Preobrazhenskii and N. Vinogradov (synchrotron MAX-Lab, Lund, Sweden) for their assistance in carrying out experiments