Effects of elevated seawater pCO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

Background: The green crab Carcinus maenas is known for its high acclimation potential to varying environmental abiotic conditions. A high ability for ion and acid-base regulation is mainly based on an efficient regulation apparatus located in gill epithelia. However, at present it is neither known which ion transport proteins play a key role in the acid-base compensation response nor how gill epithelia respond to elevated seawater pCO2 as predicted for the future. In order to promote our understanding of the responses of green crab acid-base regulatory epithelia to high pCO2, Baltic Sea green crabs were exposed to a pCO2 of 400 Pa. Gills were screened for differentially expressed gene transcripts using a 4,462-feature microarray and quantitative real-time PCR. Results: Crabs responded mainly through fine scale adjustment of gene expression to elevated pCO2. However, 2% of all investigated transcripts were significantly regulated 1.3 to 2.2-fold upon one-week exposure to CO2 stress. Most of the genes known to code for proteins involved in osmo- and acid-base regulation, as well as cellular stress response, were were not impacted by elevated pCO2. However, after one week of exposure, significant changes were detected in a calcium-activated chloride channel, a hyperpolarization activated nucleotide-gated potassium channel, a tetraspanin, and an integrin. Furthermore, a putative syntaxin-binding protein, a protein of the transmembrane 9 superfamily, and a Cl-/HCO3 - exchanger of the SLC 4 family were differentially regulated. These genes were also affected in a previously published hypoosmotic acclimation response study. Conclusions: The moderate, but specific response of C. maenas gill gene expression indicates that (1) seawater acidification does not act as a strong stressor on the cellular level in gill epithelia; (2) the response to hypercapnia is to some degree comparable to a hypoosmotic acclimation response; (3) the specialization of each of the posterior gill arches might go beyond what has been demonstrated up to date; and (4) a re-configuration of gill epithelia might occur in response to hypercapnia

Magnetic resonance imaging (MRI): a technique to study flow an microstructure of concentrated emulsions

Nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) have recently been recognized as important techniques for R&D of products and processes, as is attested by several successful applications in different areas of chemical engineering in recent years. In this article we present new experimental methods based on MRI to study flow and microstructure of concentrated emulsions. The objective is to present the unique features of this noninvasive technique to accurately measure different properties of flowing particulate opaque systems. Experimental results of velocity profiles, spatial distribution of droplet sizes and spatial homogeneity of an oil-in-water dispersion in a horizontal, concentric cylinder geometry using different pulse sequences are presented. The application of these techniques allowed probing important information on flow and microstructure of multiphase systems of interest in chemical engineering and food science

Measurement of Triple Gauge-Boson Couplings at LEP energies up to 189 GeV

The triple gauge-boson couplings involving the W are determined using data samples collected with the ALEPH detector at mean centre-of-mass energies of 183 GeV and 189 GeV, corresponding to integrated luminosities of 57 pb^-1 and 174 pb^-1, respectively. The couplings, g^Z_1, Kappa_gamma and lambda_gamma, are measured using W-pair events, single-W production and single-gamma production. Each coupling is measured individually with the other two coupling fixed at their Standard Model value. Including ALEPH results from lower energies, the 95% confidence level intervals for the deviation to the Standard Model are -0.087 Dg^Z_1 0.141 -0.200 DKappa_gamma 0.258 -0.062 Lambda_gamma 0.147. Fits are also presented where two or all three couplings are allowed to vary. In addition, W-pair events are used to set limits on the C- or P-violating couplings g^V_4, g^V_5, Kappa_V, and Lambda_V, where V denotes either gamma or Z. No deviations from the Standard Model expectations are observed