Calibration of a single atom detector for atomic micro chips

We experimentally investigate a scheme for detecting single atoms magnetically trapped on an atom chip. The detector is based on the photoionization of atoms and the subsequent detection of the generated ions. We describe the characterization of the ion detector with emphasis on its calibration via the correlation of ions with simultaneously generated electrons. A detection efficiency of 47.8% (+-2.6%) is measured, which is useful for single atom detection, and close to the limit allowing atom counting with sub-Poissonian uncertainty

Indium joints for cryogenic gravitational wave detectors

A viable technique for the preparation of highly thermal conductive joints between sapphire components in gravitational wave detectors is presented. The mechanical loss of such a joint was determined to be as low as 2 × 10−3 at 20 K and 2 × 10−2 at 300 K. The thermal noise performance of a typical joint is compared to the requirements of the Japanese gravitational wave detector, KAGRA. It is shown that using such an indium joint in the suspension system allows it to operate with low thermal noise. Additionally, results on the maximum amount of heat which can be extracted via indium joints are presented. It is found that sapphire parts, joined by means of indium, are able to remove the residual heat load in the mirrors of KAGRA

A comparative analysis of the effectiveness of flood management measures based on the concept of retaining water in the landscape in different European hydro-climatic regions

In this paper, we analyse the effectiveness of flood management measures based on the concept known as retaining water in the landscape . The investigated measures include afforestation, micro-ponds and small-reservoirs. A comparative and model-based methodological approach has been developed and applied for three meso-scale catchments located in different European hydro-climatological regions: Poyo (184 km2) in the Spanish Mediterranean, Upper Iller (954 km2) in the German Alps and Kamp (621 km2) in Northeast-Austria representing the Continental hydroclimate. This comparative analysis has found general similarities in spite of the particular differences among studied areas. In general terms, the flood reduction through the concept of retaining water in the landscape depends on the following factors: the storage capacity increase in the catchment resulting from such measures, the characteristics of the rainfall event, the antecedent soil moisture condition and the spatial distribution of such flood management measures in the catchment. In general, our study has shown that, this concept is effective for small and medium events, but almost negligible for the largest and less frequent floods: this holds true for all different hydro-climatic regions, and with different landuse, soils and morphological settings.Most of this research was done within the ERANET-Crue project "Room for the River", which was sponsored by: Lebensministerium (Austria), Bundesministerium fur Bildung und Forschung (Germany) and Ministerio de Ciencia y Educacion (Spain). 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Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany

BackgroundArthropod-borne diseases remain a major health-threat for humans and animals worldwide. To estimate the distribution of pathogenic agents and especially Bartonella spp., we conducted tick microbiome analysis and determination of the infection status of wild animals, pets and pet owners in the state of Hesse, Germany.ResultsIn total, 189 engorged ticks collected from 163 animals were tested. Selected ticks were analyzed by next generation sequencing (NGS) and confirmatory PCRs, blood specimens of 48 wild animals were analyzed by PCR to confirm pathogen presence and sera of 54 dogs, one cat and 11 dog owners were analyzed by serology. Bartonella spp. were detected in 9.5% of all ticks and in the blood of 17 roe deer. Further data reveal the presence of the human and animal pathogenic species of genera in the family Spirochaetaceae (including Borrelia miyamotoi and Borrelia garinii), Bartonella spp. (mainly Bartonella schoenbuchensis), Rickettsia helvetica, Francisella tularensis and Anaplasma phagocytophilum in ticks. Co-infections with species of several genera were detected in nine ticks. One dog and five dog owners were seropositive for anti-Bartonella henselae-antibodies and one dog had antibodies against Rickettsia conorii.ConclusionsThis study provides a snapshot of pathogens circulating in ticks in central Germany. A broad range of tick-borne pathogens are present in ticks, and especially in wild animals, with possible implications for animal and human health. However, a low incidence of Bartonella spp., especially Bartonella henselae, was detected. The high number of various detected pathogens suggests that ticks might serve as an excellent sentinel to detect and monitor zoonotic human pathogens

Microbiome Analysis Reveals the Presence of Bartonella spp. and Acinetobacter spp. in Deer Keds (Lipoptena cervi)

The deer ked (Lipoptena cervi) is distributed in Europe, North America, and Siberia and mainly infests cervids as roe deer, fallow deer, and moose. From a one health perspective, deer keds occasionally bite other animals or humans and are a potential vector for Bartonella schoenbuchensis. This bacterium belongs to a lineage of ruminant-associated Bartonella spp. and is suspected to cause dermatitis and febrile diseases in humans. In this study, we analyzed the microbiome from 130 deer keds collected from roe deer, fallow deer and humans in the federal states of Hesse, Baden-Wuerttemberg, and Brandenburg, Germany. Endosymbiontic Arsenophonus spp. and Bartonella spp. represented the biggest portion (~90%) of the microbiome. Most Bartonella spp. (n = 93) were confirmed to represent B. schoenbuchensis. In deer keds collected from humans, no Bartonella spp. were detected. Furthermore, Acinetobacter spp. were present in four samples, one of those was confirmed to represent A. baumannii. These data suggest that deer keds harbor only a very narrow spectrum of bacteria which are potentially pathogenic for animals of humans

Anastral spindle assembly and γ-tubulin in Drosophila oocytes

<p>Abstract</p> <p>Background</p> <p>Anastral spindles assemble by a mechanism that involves microtubule nucleation and growth from chromatin. It is still uncertain whether γ-tubulin, a microtubule nucleator essential for mitotic spindle assembly and maintenance, plays a role. Not only is the requirement for γ-tubulin to form anastral <it>Drosophila </it>oocyte meiosis I spindles controversial, but its presence in oocyte meiosis I spindles has not been demonstrated and is uncertain.</p> <p>Results</p> <p>We show, for the first time, using a bright GFP fusion protein and live imaging, that the <it>Drosophila </it>maternally-expressed γTub37C is present at low levels in oocyte meiosis I spindles. Despite this, we find that formation of bipolar meiosis I spindles does not require functional γTub37C, extending previous findings by others. Fluorescence photobleaching assays show rapid recovery of γTub37C in the meiosis I spindle, similar to the cytoplasm, indicating weak binding by γTub37C to spindles, and fits of a new, potentially more accurate model for fluorescence recovery yield kinetic parameters consistent with transient, diffusional binding.</p> <p>Conclusions</p> <p>The FRAP results, together with its mutant effects late in meiosis I, indicate that γTub37C may perform a role subsequent to metaphase I, rather than nucleating microtubules for meiosis I spindle formation. Weak binding to the meiosis I spindle could stabilize pre-existing microtubules or position γ-tubulin for function during meiosis II spindle assembly, which follows rapidly upon oocyte activation and completion of the meiosis I division.</p

Association between social support and place of delivery: a cross-sectional study in Kericho, Western Kenya

Background: An estimated 358,000 maternal deaths still occur worldwide each year. The place of delivery is of great significance to the reduction of maternal mortality. Moreover, socio-economic factors, cultural traits, and local customs are associated with health-seeking behavior. This study aimed to explore determinants of association between social support and place of delivery. Methods: This cross-sectional study was conducted from September to November 2011 at Sosiot Health Center, Kericho West District, Kenya. Participants were 303 mothers who brought their babies to the health center for immunization within their first year of life. Women underwent a structured interview using a questionnaire on demographic characteristics and their experiences of delivery including place of delivery and social support. Results: The proportion of deliveries at health facilities was significantly higher in unmarried than married women (93% and 78%, respectively; P = 0.008). Unmarried women whose mothers supported them in housework and whose sisters helped them fetch water were more likely to deliver at health facilities (P = 0.002 and 0.042, respectively) than those without this support. However, married women whose husbands supported them in farming and whose neighbors helped them fetch water were less likely to deliver at health facilities (P = 0.003 and 0.021, respectively) than those without this support. Married women who were advised to deliver at a health facility by their mother-in-law or health staff were more likely to deliver at health facilities (P = 0.015 and 0.022, respectively) than those who did not receive this advice. Multivariate analysis revealed that married women were more likely to deliver at health facilities if they were highly educated (odds ratio [OR] = 2.5); had financial capability (OR = 4.3); had medical insurance (OR = 4.2); were primiparous (OR = 3.5); did not have the support of sisters-in-law for fetching water (OR = 2.2); or were advised to deliver at a health facility by family or neighbors (OR = 2.5).Conclusions: Promotion of delivery at health facilities requires approaches that consider women\u27s social situation, since factors influencing place of delivery differ for married and unmarried women

Ensemble prediction of floods &ndash; catchment non-linearity and forecast probabilities

Quantifying the uncertainty of flood forecasts by ensemble methods is becoming increasingly important for operational purposes. The aim of this paper is to examine how the ensemble distribution of precipitation forecasts propagates in the catchment system, and to interpret the flood forecast probabilities relative to the forecast errors. We use the 622 km² Kamp catchment in Austria as an example where a comprehensive data set, including a 500 yr and a 1000 yr flood, is available. A spatially-distributed continuous rainfall-runoff model is used along with ensemble and deterministic precipitation forecasts that combine rain gauge data, radar data and the forecast fields of the ALADIN and ECMWF numerical weather prediction models. The analyses indicate that, for long lead times, the variability of the precipitation ensemble is amplified as it propagates through the catchment system as a result of non-linear catchment response. In contrast, for lead times shorter than the catchment lag time (e.g. 12 h and less), the variability of the precipitation ensemble is decreased as the forecasts are mainly controlled by observed upstream runoff and observed precipitation. Assuming that all ensemble members are equally likely, the statistical analyses for five flood events at the Kamp showed that the ensemble spread of the flood forecasts is always narrower than the distribution of the forecast errors. This is because the ensemble forecasts focus on the uncertainty in forecast precipitation as the dominant source of uncertainty, and other sources of uncertainty are not accounted for. However, a number of analyses, including Relative Operating Characteristic diagrams, indicate that the ensemble spread is a useful indicator to assess potential forecast errors for lead times larger than 12 h