Far Infrared Observations of the Galactic Star Forming Regions associated with IRAS 00338+6312 and RAFGL 5111

Two Galactic star forming regions, one in a very early phase of evolution and another evolved one, associated with the IRAS sources 00338+6312 and 03595+5110 (RAFGL 5111) respectively have been studied in detail. These sources have been mapped simultaneously in two far infrared bands at 143 & 185 \micron), with about 1.5 arcmin angular resolution, using the TIFR 100 cm balloon borne telescope. The HIRES processed IRAS maps at 12, 25, 60 & 100 \micron, have been used for comparison. Whereas IRAS 00338+6312 is resolved only in the TIFR bands, RAFGL 5111 is very well resolved in both the TIFR bands, as well as in at least 3 IRAS bands. The neighbouring fainter source IRAS 04004+5114 has also been resolved in the TIFR bands. Taking advantage of the identical beams in the two TIFR bands at 143 & 185 \micron, dust colour temperature, $T(143/185)$, and optical depth, $\tau_{150}$, maps have been generated for RAFGL 5111. These maps show interesting structural details. Radiative transfer modelling in spherical geometry has been carried out for individual sources. The best fit models are in good agreement with the observed spectral energy distribution (SED), radio continuum data etc. Another scheme of radiative transfer through the interstellar dust-gas cloud including the heavier elements has been used to predict ionic nebular line emission, which are in reasonable agreement with the measurements for RAFGL 5111. An important conclusion from the present study is that, for all the three sources (IRAS 00338+6312; 03595+5110; and 04004+5114, a faint source in the neighbourhood of RAFGL 5111), the best fit to the observed SED is obtained for a uniform density ($n(r) \sim r^0$) cloud

Comparison of Friedewald’s formula, modified Friedewald’s formula and Anandaraja’s formula with direct homogenous serum LDL cholesterol method in CHD patients

Background: Elevated serum Low-Density Lipoprotein Cholesterol (LDL-C) concentration is a well-known atherogenic risk factor with a high predictive value for coronary heart disease. An important aspect of the assessment of coronary heart disease risk for a dyslipidemic subject is the estimation of serum Low-Density Lipoprotein Cholesterol (LDL-C). There are many homogenous assays currently available for the estimation of serum LDL-C. Most clinical laboratories determine LDL-C (mg/dl) by Friedewald’s formula (FF), LD-=(TC)-HDL-C)-(TG/5), Modified Friedewald’s formula (MFF), LDL-C=(TC)-(HDL-C)-(TG/6), Recently Anandaraja and colleagues have derived a new formula for calculating LDL-C, AR-LDL-C=0.9 TC-(0.9 TG/5)-28.Methods: It is cross-sectional study. Lipid profile data was collected from known of CHD patients, who had come for lipid profile investigation to the Central Biochemistry laboratory of ACPM Medical College and hospital. LDL-C estimation was done by direct homogenous assay and also calculated using the Friedewald’s Formula, Modified Friedewald’s Formula and Anandaraja’s Formula for assessing and validity of the LDL cholesterol.Results: From the present study, The LDL-FF, MFW and AR are increased with levels of TGL > 200 mg/dl and decreased level of TC < 200 mg/dl seem to interfere with the estimation of Direct LDL cholesterolConclusions: Authors conclude that, LDL-C by direct method is most reliable and sensitive in CHD patients compare with FF, MFW, and ARF

Author Correction: LKB1 loss links serine metabolism to DNA methylation and tumorigenesis

Erratum for: LKB1 loss links serine metabolism to DNA methylation and tumorigenesis. [Nature. 2016

Arabidopsis Sec1/Munc18 protein SEC11 is a competitive and dynamic modulator of SNARE binding and SYP121-dependent vesicle traffic

The Arabidopsis thaliana Qa-SNARE SYP121 (=SYR1/PEN1) drives vesicle traffic at the plasma membrane of cells throughout the vegetative plant. It facilitates responses to drought, to the water stress hormone abscisic acid, and to pathogen attack, and it is essential for recovery from so-called programmed stomatal closure. How SYP121-mediated traffic is regulated is largely unknown, although it is thought to depend on formation of a fusion-competent SNARE core complex with the cognate partners VAMP721 and SNAP33. Like SYP121, the Arabidopsis Sec1/Munc18 protein SEC11 (=KEULE) is expressed throughout the vegetative plant. We find that SEC11 binds directly with SYP121 both in vitro and in vivo to affect secretory traffic. Binding occurs through two distinct modes, one requiring only SEC11 and SYP121 and the second dependent on assembly of a complex with VAMP721 and SNAP33. SEC11 competes dynamically for SYP121 binding with SNAP33 and VAMP721, and this competition is predicated by SEC11 association with the N terminus of SYP121. These and additional data are consistent with a model in which SYP121-mediated vesicle fusion is regulated by an unusual “handshaking” mechanism of concerted SEC11 debinding and rebinding. They also implicate one or more factors that alter or disrupt SEC11 association with the SYP121 N terminus as an early step initiating SNARE complex formation

Transepithelial Transport of Fc-Targeted Nanoparticles by the Neonatal Fc Receptor for Oral Delivery

Nanoparticles are poised to have a tremendous impact on the treatment of many diseases, but their broad application is limited because currently they can only be administered by parenteral methods. Oral administration of nanoparticles is preferred but remains a challenge because transport across the intestinal epithelium is limited. We show that nanoparticles targeted to the neonatal Fc receptor (FcRn), which mediates the transport of immunoglobulin G antibodies across epithelial barriers, are efficiently transported across the intestinal epithelium using both in vitro and in vivo models. In mice, orally administered FcRn-targeted nanoparticles crossed the intestinal epithelium and reached systemic circulation with a mean absorption efficiency of 13.7%*hour compared with only 1.2%*hour for nontargeted nanoparticles. In addition, targeted nanoparticles containing insulin as a model nanoparticle-based therapy for diabetes were orally administered at a clinically relevant insulin dose of 1.1 U/kg and elicited a prolonged hypoglycemic response in wild-type mice. This effect was abolished in FcRn knockout mice, indicating that the enhanced nanoparticle transport was specifically due to FcRn. FcRn-targeted nanoparticles may have a major impact on the treatment of many diseases by enabling drugs currently limited by low bioavailability to be efficiently delivered though oral administration.Prostate Cancer Foundation (Award in Nanotherapeutics)National Cancer Institute (U.S.) (Center for Cancer Nanotechnology Excellence U54-CA151884)National Heart, Lung, and Blood Institute (Program of Excellence in Nanotechnology Award Contract HHSN268201000045C)National Institutes of Health (U.S.) (Grant EB000244)National Institutes of Health (U.S.) (R01 Grant EB015419-01)American Society for Engineering Education. National Defense Science and Engineering Graduate FellowshipNational Cancer Institute (U.S.) (Center for Cancer Nanotechnology Excellence Graduate Research Fellowship 5 U54 CA151884-02

Far and mid infrared observations of two ultracompact H II regions and one compact CO clump

Two ultracompact H II regions (IRAS 19181+1349 and 20178+4046) and one compact molecular clump (20286+4105) have been observed at far infrared wavelengths using the TIFR 1 m balloon-borne telescope and at mid infrared wavelengths using ISO. Far infrared observations have been made simultaneously in two bands with effective wavelengths of ~ 150 and ~ 210 micron, using liquid 3He cooled bolometer arrays. ISO observations have been made in seven spectral bands using the ISOCAM instrument; four of these bands cover the emission from Polycyclic Aromatic Hydrocarbon (PAH) molecules. In addition, IRAS survey data for these sources in the four IRAS bands have been processed using the HIRES routine. In the high resolution mid infrared maps as well as far infrared maps multiple embedded energy sources have been resolved. There are structural similarities between the images in the mid infrared and the large scale maps in the far infrared bands, despite very different angular resolutions of the two. Dust temperature and optical depth (tau_150 um) maps have also been generated using the data from balloon-borne observations. Spectral energy distributions (SEDs) for these sources have been constructed by combining the data from all these observations. Radiation transfer calculations have been made to understand these SEDs. Parameters for the dust envelopes in these sources have been derived by fitting the observed SEDs. In particular, it has been found that radial density distribution for three sources is diffrent. Whereas in the case of IRAS 20178+4046, a steep distribution of the form r^-2 is favoured, for IRAS 20286+4105 it is r^-1 and for IRAS 19181+1349 it the uniform distribution (r^0). Line ratios for PAH bands have generally been found to be similar to those for other compact H II regions but different from general H II regions.Comment: To appear in Astronomy & Astrophysics; (19 pages including 14 Figures and 6 Tables

Mental health issues in unaccompanied refugee minors

Previous studies about unaccompanied refugee minors (URMs) showed that they are a highly vulnerable group who have greater psychiatric morbidity than the general population. This review focuses on mental health issues among URMs. Articles in databases PsycINFO, Medline and PubMed from 1998 to 2008 addressing this topic were reviewed. The literature had a considerable emphasis on the assessment of PTSD symptoms. Results revealed higher levels of PTSD symptoms in comparison to the norm populations and accompanied refugee minors. In several studies, age and female gender predicted or influenced PTSD symptoms. The existing literature only permits limited conclusions on this very hard to reach population. Future research should include the analysis of long-term outcomes, stress management and a more thorough analysis of the whole range of psychopathology. Additionally, the development of culturally sensitive norms and standardized measures for diverse ethnic groups is of great importance

Untapped Potential of Clinical Text for Opioid Surveillance

Accurate surveillance is needed to combat the growing opioid epidemic. To investigate the potential volume of missed opioid overdoses, we compare overdose encounters identified by ICD-10-CM codes and an NLP pipeline from two different medical systems. Our results show that the NLP pipeline identified a larger percentage of OOD encounters than ICD-10-CM codes. Thus, incorporating sophisticated NLP techniques into current diagnostic methods has the potential to improve surveillance on the incidence of opioid overdoses

A new scheme of radiation transfer in H II regions including transient heating of grains

A new scheme of radiation transfer for understanding infrared spectra of H II regions, has been developed. This scheme considers non-equilibrium processes (e. g. transient heating of the very small grains, VSG; and the polycyclic aromatic hydrocarbon, PAH) also, in addition to the equilibrium thermal emission from normal dust grains (BG). The spherically symmetric interstellar dust cloud is segmented into a large number of "onion skin" shells in order to implement the non-equilibrium processes. The scheme attempts to fit the observed SED originating from the dust component, by exploring the following parameters : (i) geometrical details of the dust cloud, (ii) PAH size and abundance, (iii) composition of normal grains (BG), (iv) radial distribution of all dust (BG, VSG & PAH). The scheme has been applied to a set of five compact H II regions (IRAS 18116- 1646, 18162-2048, 19442+2427, 22308+5812 & 18434-0242) whose spectra are available with adequate spectral resolution. The best fit models and inferences about the parameters for these sources are presented.Comment: 16 pages total including 3 tables and 2 figure