Impact of sorbic acid on germinant receptor-dependent and -independent germination pathways in Bacillus cereus

Amino acid- and inosine-induced germination of Bacillus cereus ATCC 14579 spores was reversibly inhibited in the presence of 3 mM undissociated sorbic acid. Exposure to high hydrostatic pressure, Ca-dipicolinic acid (DPA), and bryostatin, an activator of PrkC kinase, negated this inhibition, pointing to specific blockage of signal transduction in germinant receptor-mediated germination

UVES and X-Shooter spectroscopy of the emission line AM CVn systems GP Com and V396 Hya

We present time-resolved spectroscopy of the AM CVn-type binaries GP Com and V396 Hya obtained with VLT/X-Shooter and VLT/UVES. We fully resolve the narrow central components of the dominant helium lines and determine radial velocity semi-amplitudes of $K_{\rm spike} = 11.7\pm0.3$ km s$^{-1}$ for GP Com and $K_{\rm spike} = 5.8\pm0.3$ km s$^{-1}$ for V396 Hya. The mean velocities of the narrow central components show variations from line to line. Compared to calculated line profiles that include Stark broadening we are able to explain the displacements, and the appearance of forbidden helium lines, by additional Stark broadening of emission in a helium plasma with an electron density $n_e\simeq 5\times 10^{15}$ cm$^{-3}$. More than $30$ nitrogen and more than $10$ neon lines emission lines were detected in both systems. Additionally, $20$ nitrogen absorption lines are only seen in GP Com. The radial velocity variations of these lines show the same phase and velocity amplitude as the central helium emission components. The small semi-amplitude of the central helium emission component, the consistency of phase and amplitude with the absorption components in GP Com as well as the measured Stark effect shows that the central helium emission component, the so-called central-spike, is consistent with an origin on the accreting white dwarf. We use the dynamics of the bright spot and the central spike to constrain the binary parameters for both systems and find a donor mass of $9.6$ - $42.8$ M$_{\rm Jupiter}$ for GP Com and $6.1$ - $30.5$ M$_{\rm Jupiter}$ for V396 Hya. We find an upper limit for the rotational velocity of the accretor of $v_{\rm rot}<46$ km s$^{-1}$ for GP Com and $v_{\rm rot}<59$ km s$^{-1}$ for V396 Hya which excludes a fast rotating accretor in both systems.Comment: Accepted for publication in MNRAS, 15 pages, 14 figures, 5 table