33 research outputs found
The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
Many bacteria produce and respond to the quorum sensing signal autoinducer-2 (AI-2). Escherichia coli and Salmonella typhimurium are among the species with the lsr operon, an operon containing AI-2 transport and processing genes that are up regulated in response to AI-2. One of the Lsr proteins, LsrF, has been implicated in processing the phosphorylated form of AI-2. Here, we present the structure of LsrF, unliganded and in complex with two phospho-AI-2 analogues, ribose-5-phosphate and ribulose-5-phosphate. The crystal structure shows that LsrF is a decamer of (αβ)8-barrels that exhibit a previously unseen N-terminal domain swap and have high structural homology with aldolases that process phosphorylated sugars. Ligand binding sites and key catalytic residues are structurally conserved, strongly implicating LsrF as a class I aldolase
Improving carbon dioxide capture by ionic liquids : effect of the fluorination of the cation
Communication oral
De la biocatalyse à la catalyse hybride, les enzymes au cœur de procédés sélectifs et durables
National audienc
De la biocatalyse à la catalyse hybride, les enzymes au cœur de procédés sélectifs et durables
National audienc
De la biocatalyse à la catalyse hybride, les enzymes au cœur de procédés sélectifs et durables
National audienc
Enzyme encapsulation in Water/Water Pickering emulsion vesicles stabilized by Layered Double Hydroxides nanoparticles.
International audienc
Continuous colorimetric screening assays to explore both acceptor and donor substrates of amine-Transaminases.
International audienc