The Shapes of Dense Cores and Bok Globules

The shapes of isolated Bok globules and embedded dense cores of molecular clouds are analyzed using a nonparametric method, under the alternate hypotheses that they are randomly oriented prolate objects or that they are randomly oriented oblate objects. In all cases, the prolate hypothesis gives a better fit to the data. If Bok globules are oblate, they must be very flat; the average axis ratio is b/a = 0.3, and few or no globules can have b/a > 0.7. If Bok globules are prolate, then the mean axis ratio is b/a = 0.5. For most data samples of dense cores, the randomly-oriented oblate hypothesis can be rejected at the 99% confidence level. If the dense cores are prolate, their mean axis ratio is approximately 0.4 to 0.5. Dense cores are significantly different in shape from the clouds in which they are embedded; clouds have flatter apparent shapes, and are inconsistent with a population of randomly oriented axisymmetric objects.Comment: 26 pages (LaTeX) including 8 postscript figures; to appear in Ap

Annual Report 2022 - Institute of Resource Ecology

The Institute of Resource Ecology (IRE) is one of the ten institutes of the Helmholtz-Zentrum Dresden – Rossendorf (HZDR). Our research activities are mainly integrated into the program “Nuclear Waste Management, Safety and Ra-diation Research (NUSAFE)” of the Helmholtz Association (HGF) and focus on the topics “Safety of Nuclear Waste Disposal” and “Safety Research for Nuclear Reactors”. The program NUSAFE, and therefore all work which is done at IRE, belong to the research field “Energy” of the HGF

Ultrasensitive detection of lipoarabinomannan with plasmonic grating biosensors in clinical samples of HIV negative patients with tuberculosis.

BACKGROUND:Timely diagnosis of tuberculosis disease is critical for positive patient outcomes, yet potentially millions go undiagnosed or unreported each year. Sputum is widely used as the testing input, but limited by its complexity, heterogeneity, and sourcing problems. Finding methods to interrogate noninvasive, non-sputum clinical specimens is indispensable to improving access to tuberculosis diagnosis and care. In this work, economical plasmonic gratings were used to analyze tuberculosis biomarker lipoarabinomannan (LAM) from clinical urine samples by single molecule fluorescence assay (FLISA) and compared with gold standard sputum GeneXpert MTB/ RIF, culture, and reference ELISA testing results. METHODS AND FINDINGS:In this study, twenty sputum and urine sample sets were selected retrospectively from a repository of HIV-negative patient samples collected before initiation of anti-tuberculosis therapy. GeneXpert MTB/RIF and culture testing of patient sputum confirmed the presence or absence of pulmonary tuberculosis while all patient urines were reference ELISA LAM-negative. Plasmonic gratings produced by low-cost soft lithography were bound with anti-LAM capture antibody, incubated with patient urine samples, and biotinylated detection antibody. Fluorescently labeled streptavidin revealed single molecule emission by epifluorescence microscope. Using a 1 fg/mL baseline for limit of detection, single molecule FLISA demonstrated good qualitative agreement with gold standard tests on 19 of 20 patients, including accurately predicting the gold-standard-negative patients, while one gold-standard-positive patient produced no observable LAM in urine. CONCLUSIONS:Single molecule FLISA by plasmonic grating demonstrated the ability to quantify tuberculosis LAM from complex urine samples of patients from a high endemic setting with negligible interference from the complex media itself. Moreover, agreement with patient diagnoses by gold standard testing suggests that single molecule FLISA could be used as a highly sensitive test to diagnose tuberculosis noninvasively

Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration.

Recessive Stargardt disease (STGD1) is an inherited blinding disorder caused by mutations in the Abca4 gene. ABCA4 is a flippase in photoreceptor outer segments (OS) that translocates retinaldehyde conjugated to phosphatidylethanolamine across OS disc membranes. Loss of ABCA4 in Abca4 -/- mice and STGD1 patients causes buildup of lipofuscin in the retinal pigment epithelium (RPE) and degeneration of photoreceptors, leading to blindness. No effective treatment currently exists for STGD1. Here we show by several approaches that ABCA4 is additionally expressed in RPE cells. (i) By in situ hybridization analysis and by RNA-sequencing analysis, we show the Abca4 mRNA is expressed in human and mouse RPE cells. (ii) By quantitative immunoblotting, we show that the level of ABCA4 protein in homogenates of wild-type mouse RPE is about 1% of the level in neural retina homogenates. (iii) ABCA4 immunofluorescence is present in RPE cells of wild-type and Mertk -/- but not Abca4 -/- mouse retina sections, where it colocalizes with endolysosomal proteins. To elucidate the role of ABCA4 in RPE cells, we generated a line of genetically modified mice that express ABCA4 in RPE cells but not in photoreceptors. Mice from this line on the Abca4 -/- background showed partial rescue of photoreceptor degeneration and decreased lipofuscin accumulation compared with nontransgenic Abca4 -/- mice. We propose that ABCA4 functions to recycle retinaldehyde released during proteolysis of rhodopsin in RPE endolysosomes following daily phagocytosis of distal photoreceptor OS. ABCA4 deficiency in the RPE may play a role in the pathogenesis of STGD1

Grizzly Bear Scavenging of Carrion on the Northern Yellowstone Winter Range (1997-2012)

The Northern Yellowstone Winter Range (NYWR) in northwestern Wyoming and southwestern Montana is an important winter migratory destination for ungulates.  The NYWR is within the Greater Yellowstone Ecosystem (GYE), a landscape characterized by a complex ecological system of predators, scavengers, and ungulates.  Grizzly bears (Ursus arctos) are dominant members of the scavenging community throughout the spring.  However, little is known about factors associated with grizzly bear use of carcasses.  Of particular interest to managers is how habitat and anthropogenic factors are associated with carcass use.  Such information, for example, may be useful to manage spring recreation in important bear foraging areas to reduce conflict and support conservation efforts.  We used logistic regression to analyze spring survey data from 23 transects located in Yellowstone National Park and the Gallatin National Forest during 1997–2012, to identify factors associated with grizzly bear scavenging of winter- or predator-killed ungulates.  Multi-model inference was used to evaluate relative support for a set of a priori candidate models containing environmental and temporal correlates. Our preliminary findings showed support for models with distance to forest edge, road density, and elevation. Results indicated negative relationships between these factors and probability of carcass use.  Our results suggest that spatial heterogeneity in landscape-level habitat characteristics and human activity affect grizzly bear use of a valuable spring food source

Annual Report 2013 - Institute of Resource Ecology

The Institute of Resource Ecology (IRE) ISone of the eight institutes of the Helmholtz-Zentrum Dresden-Rossendorf (HZDR). The Research activities are mainly integrated into the program “Nuclear Safety Research (NUSAFE)” of the Helmholtz Association (HGF) and focused on the topics “Safety of Nuclear Waste Disposal” and “Safety Research for Nuclear Reactors”. Additionally, various activities have been started investigating chemical and environmental aspects of processing and recycling of strategic metals, namely rare earth elements. These activities are located in the HGF program “Energy Efficiency, Materials and Resources (EMR)”. Both programs, and therefore all work which is done at IRE, belong to the research sector “Energy” of the HGF. The research objectives are the protection of humans and the environment from hazards caused by pollutants resulting from technical processes that produce energy and raw materials. Treating technology and ecology as a unity is the major scientific challenge in assuring the safety of technical processes and gaining their public acceptance. Namely, we investigate the ecological risks exerted by radioactive and non-radioactive metals in the context of nuclear waste disposal, the production of energy in nuclear power plants and in processes along the value chain of metalliferous raw materials. A common goal is to generate better understanding about the dominating processes essential for metal mobilization and immobilization on the molecular level. This in turn enables us to assess the macroscopic phenomena, including models, codes and data for predictive calculations, which determine the transport and distribution of contaminants in the environment

Spin-triplet superconductivity in quasi-one dimension

We consider a system with electron-phonon interaction, antiferromagnetic fluctuations and disconnected open Fermi surfaces. The existence of odd-parity superconductivity in this circumstance is shown for the first time. If it is applied to the quasi-one-dimensional systems like the organic conductors (TMTSF)_2X we obtain spin-triplet superconductivity with nodeless gap. Our result is also valid in higher dimensions(2d and 3d).Comment: 2 page

Transforming Post-Secondary Education in Mathematics

In this manuscript, I introduce and describe the work of mathematicians and mathematics educators in the group Transforming Post-Secondary Education in Mathematics (TPSE Math or TPSE, pronounced "tipsy", for short). TPSE aims to coordinate and drive constructive change in education in the mathematical sciences at two-year colleges, four-year colleges, and universities across the nation. It seeks to build on the successes of the entire mathematical sciences community. This manuscript reviews the events that led to the founding of TPSE Math and articulates its vision and mission. In its first phase with national events, TPSE found broad consensus with the mathematical sciences community on the challenges facing the community. Learning from educational transformations experiences in other scientific fields, and with the support of the Mathematical Advisory Group of 34 mathematical sciences department chairs and leaders, TPSE moves into a second phase focused on action. This is a snapshot in time, and TPSE's ongoing activities will continue to be documented and disseminated. The piece concludes with a reflection of the impact that my involvement in this work has had on my career.Comment: 17 page

Shifts of the nuclear resonance in the vortex lattice in YBa2_2Cu3_3O7_7

The NMR and NQR spectra of $^{63}$Cu in the CuO$_2$ plane of YBa$_2$Cu$_3$O$_7$ in the superconducting state are discussed in terms of the phenomenological theory of Ginzburg-Landau type extended to lower temperatures. We show that the observed spectra, Kumagai {\em et al.}, PRB {\bf 63}, 144502 (2001), can be explained by a standard theory of the Bernoulli potential with the charge transfer between CuO$_2$ planes and CuO chains assumed.Comment: 11 pages 7 figure

A Galactic Plane Relative Extinction Map from 2MASS

We present three 14400 square degree relative extinction maps of the Galactic Plane (|b|<20degrees) obtained from 2MASS using accumulative star counts (Wolf diagrams). This method is independent of the colour of the stars and the variation of extinction with wavelength. Stars were counted in 3.5'x3.5' boxes, every 20". 1x1degree surrounding fields were chosen for reference, hence the maps represent local extinction enhancements and ignore any contribution from the ISM or very large clouds. Data reduction was performed on a Beowulf-type cluster (in approximately 120 hours). Such a cluster is ideal for this type of work as areas of the sky can be independently processed in parallel. We studied how extinction depends on wavelength in all of the high extinction regions detected and within selected dark clouds. On average a power law opacity index (\beta) of 1.0 to 1.8 in the NIR was deduced. The index however differed significantly from region to region and even within individual dark clouds. That said, generally it was found to be constant, or to increase, with wavelength within a particular region.Comment: 4 pages, 2 figures, A&A Letters accepted, version with high resolution figures at http://www.dias.ie/~d