Digestibilidade de gerações de perfilhos do capim Tanzânia.

O objetivo do presente experimento foi determinar o efeito da intensidade e do ciclo de pastejo sobre a digestibilidade in vitro da matéria orgânica de gerações de perfilhos de capim Tanzânia. O delineamento experimental foi de blocos completos ao acaso com três tratamentos (intensidade de pastejo alta, média e baixa) e quatro repetições. As gerações de perfilhos eram identificadas com fios de arame coloridos. Um dia antes de cada pastejo duas amostras eram cortadas por parcela e a digestibilidade in vitro da matéria orgânica de cada geração determinada. A digestibilidade das gerações de perfilhos foi maior nas áreas submetidas a elevada intensidade de pastejo e decresceu com o avanço da idade (redução de 3 a 6 pontos percentuais de digestibilidade por ciclo) .Os efeitos negativos do florescimento sobre o valor nutritivo da forragem podem ser reduzidos através de práticas de manejo que promovam uma maior renovação de perfilhos

Controle genético da resistência a murcha-de-fusário em feijoeiro comum.

Os objetivos do trabalho foram avaliar o controle genético da reação ao agente causal da murcha-de-fusário, a partir dos cruzamentos Milionário 1732 x Macanudo e FT Tarumã x Macanudo, e estimar parâmetros genéticos que permitam selecionar famílias resistentes à doença

El balneario de Busot (1780-1820)

Durante los últimos años del siglo XVIII y primeros del XIX, los Baños de Busot, pertenecientes al término municipal de Alicante, experimentaron una transformación con repercusiones tanto en el ámbito sanitario como social. El consistorio dirigió una serie de obras, mediante las cuales su ruinoso estado evolucionó hasta que en 1818, se abrieron oficialmente al público sus instalaciones. Tanto la procedencia de las personas que acudían a estos Baños como los oficios que se generaron en ellos, nos permiten acceder a un mayor conocimiento de la sociedad alicantina.During the last years of the eighteeth century and early nineteenth, the «Baths of Busot» belonged to the municipality of Alicante and experimented a transformation with implications both in health as social background. The municipality consistory channels a series of works and by its, the ruinous state of the spa evolved. In 1818, the spa was officially opened to the public. The study of people who went to the baths and who worked there, gives us a greater understanding of the Alicante society

Factors Related to Aedes aegypti (Diptera: Culicidae) Populations and Temperature Determine Differences on Life-History Traits With Regional Implications in Disease Transmission

Aedes aegypti (L.) (Diptera: Culicidae) is a vector of many medically significant viruses in the Americas, including dengue virus, chikungunya virus, and Zika virus. Traits such as longevity, fecundity, and feeding behavior contribute to the ability of Ae. aegypti to serve as a vector of these pathogens. Both local environmental factors and population genetics could contribute to variability in these traits. We performed a comparative study of Ae. aegypti populations from four geographically and environmentally distinct collection sites in Argentina in which the cohorts from each population were held at temperature values simulating a daily cycle, with an average of 25°C in order to identify the influence of population on life-history traits. In addition, we performed the study of the same populations held at a daily temperature cycle similar to that of the surveyed areas. According to the results, Aguaray is the most outstanding population, showing features that are important to achieve high fitness. Whereas La Plata gathers features consistent with low fitness. Iguazu was outstanding in blood-feeding rate while Posadas's population showed intermediate values. Our results also demonstrate that climate change could differentially affect unique populations, and that these differences have implications for the capacity for Ae. aegypti to act as vectors for medically important arboviruses.Fil: Muttis, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Chuchuy, Ailen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Mangudo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Ciota, Alexander. Wadsworth Center, New York State Department Of Health; Estados UnidosFil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentin

Mapeamento genético da resistência a murcha do fusario em feijoeiro comum.

Este trabalho objetivou identificar marcadores microssatélites ligados a genes de resistência a Fusarium oxysporum f. sp. phaseoli em feijoeiro comum

Marcadores moleculares funcionais associados com resistência a murcha de fusário em feijoeiro comum.

Este trabalho objetivou selecionar marcadores microssatélites polimórficos em populações segregantes derivadas dos cruzamentos entre cultivares resistentes (Milionário 1732 ou FT Tarumã) e susceptível (Macanudo) à murcha do fusário

A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.).

Sugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selectio

Real-time monitoring in three-dimensional hepatocytes reveals that insulin acts as a synchronizer for liver clock

Resetting the peripheral clock and understanding the integration between the circadian rhythm and metabolic pathways are fundamental questions. To test whether insulin acts as a synchronizer for the hepatic clock by cell-autonomous mechanisms, the phase-resetting capabilities of insulin were investigated in cultured hepatic cells. We provide evidence that three-dimensional (3D) cell culture conditions that preserve the differentiated state of primary hepatocytes sustained the robustness of the molecular clock, while this robustness rapidly dampened under classical monolayer cell culture conditions. Herein, we established a 3D cell culture system coupled with a real-time luciferase reporter, and demonstrated that insulin directly regulates the phase entrainment of hepatocyte circadian oscillators. We found that insulin-deficient diabetic rats had a pronounced phase advance in their hepatic clock. Subsequently, a single administration of insulin induced phase-dependent bi-directional phase shifts in diabetic rat livers. Our results clearly demonstrate that insulin is a liver clock synchronizer

Complex and unexpected dynamics in simple genetic regulatory networks

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