7 research outputs found

    Neuronal protective effects of focal ischemic pre-and/or postconditioning on the model of transient focal cerebral ischemia in rats

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    We investigated the neuroprotective effects of pre-and postconditioning on infarct volume in the transient middle cerebral artery occlusion (MCAo) model in rats. Thirty-two male rats were divided into occlusion, preconditioning, postconditioning and both pre- and postconditioning groups. MCAo (120 minutes) was monitored with continuous cerebral tissue oxygen (O-2) pressure (PtiO(2)). Pre-conditioning comprised 10 minutes of MC-Ao, 24 hours prior to the 120 minute MCAo. The postconditioning algorithm was 30 seconds of reperfusion followed by 30 seconds of MCAo. This cycle was repeated 3 times at the onset of reperfusion. Comparison of infarct volumes showed a significant difference between the conditioned groups and occlusion group. Although there was better protection in the preconditioning group compared with the other two conditioned groups, the results did not reach statistically significant levels. The results suggest that preconditioning, postconditioning and pre/post conditioning have protective effects on cerebral ischemia

    Effects of citicoline used alone and in combination with mild hypothermia on apoptosis induced by focal cerebral ischemia in rats

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    The effects of citicoline used either alone or in combination with hypothermia on the suppression of apoptotic processes after transient focal cerebral ischemia were investigated. Middle cerebral artery occlusion (MCAo) was performed for 2 hours on Sprague-Dawley (SD) rats using intraluminal thread insertion. The treatment groups were as follows: Group 1, sham-operate 1; Group 2, saline; Group 3, citicoline (400 mg/kg intraperitoneal.); Group 4, hypothermia (34 +/- 1 degrees C); Group 5, citicoline + hypothermia. All rats were reperfused for 24 hours, and after sacrifice and transcardiac perfusion, immunohistochemical (mean standard deviation, 0.71 +/- 0.75) was lower compared to Groups 3, 4 and 5 (2.33 +/- 0.81; 3.00 +/- 0.00; 2.20 +/- 0.83; p < 0.05). There was higher expression of cispase-3 proteins in Group 2 (2.28 +/- 0.95) compared to Group 5 (1.50 +/- 0.83; p < 0.05). Bax proteins were also increased in Group 2 (1.85 +/- 1.06) compared to Group 5 (0.40 +/- 0.54) and in Group 4 (2.00 +/- 0.00) compared to Group 5 (0.40 +/- 0.54; p < 0.05). Significant differences in caspase-9 immunostaining scores were found in Group 2 (2.29 +/- 0.96) compared to Group 5 (0.20 +/- 0.44) (p < 0.05); Group 3 (1.00 +/- 0.70) compared to Group 5 (0.20 +/- 0.44: p, < 0.05); and Group 4 (3.00 +/- 0.00; p < 0.05) compared to Group 5 (0.40 +/- 0.54; p < 0.05). Thus by suppressing apoptotic processes citicoline with hypothermia is more effective than ;either used alone in ameliorating cerebral damage after transient focal ischemia

    Single or multiple small subarachnoid hemorrhages by puncturing a small branch of the rat basilarartery causes chronic cerebral vasospasm

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    OBJECTIVE: This study looked at the effects of single and multiple small subarachnoid hemorrhage (SAH) caused by puncturing a small branch of the basilar artery in rats. METHODS: Rats were subjected to single SAH (n = 21), multiple SAH (n = 21), sham operation (n = 21), or no procedures (control group, n = 7). SAH was induced in rats by tranclival puncture of a small branch of the basilar artery. In the multiple-SAH hemorrhage-groups, three small hemorrhages were produced in the same artery at three different times (initial and 24 and 48 h). In the single-SAH groups, one small hemorrhage was produced. Measurements of local cerebral blood flow (LCBF) were made at the initial SAH procedure and at three different time points. Seven animals from each general grouping were killed on Days 4, 10, and 14 (after LCBF was measured). Three different levels of the basilar artery were examined in each animal. Luminal area and arterial wall thickness were measured, and the findings were compared with control and corresponding sham groups findings. RESULTS: LCBF dropped dramatically (by 40%) immediately after SAH and reached levels near baseline within 15 minutes (n = 42) (P < 0.001). LCBF continued to drop after initial SAH and reached the lowest level on Day 10 (P < 0.001) or Day 14 (P < 0.05). Significant luminal narrowing (P < 0.01) and thickening of the arterial wall (P < 0.01) were observed in both groups. CONCLUSION: Single or multiple small SAHs produced by puncturing the basilar artery in the rat cause similar acute and chronic cerebral vasospasm
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