Decay of Hypernuclei

We present a nonrelativistic transition potential for the weak strangeness-changing reaction $\Lambda N \to NN$. The potential is based on a one meson exchange model (OME), where, in addition to the long-ranged pion, the exchange of the pseudoscalar $K, \eta$, as well as the vector $\rho, \omega, K^*$ mesons is considered. Results obtained for different hypernuclear decay observables are compared to the available experimental data.Comment: 8 pages. Invited talk given at the KEK-Tanashi International Symposium on Physics of Hadrons and Nuclei. Tokyo, Japan, December 14-17, 1998. In honor of Prof. K. Yazaki. Submitted to Nucl. Phys. A. LateX file (uses espcrc1.sty

The pestivirus N terminal protease N(pro) redistributes to mitochondria and peroxisomes suggesting new sites for regulation of IRF3 by N(pro.)

The N-terminal protease of pestiviruses, N(pro) is a unique viral protein, both because it is a distinct autoprotease that cleaves itself from the following polyprotein chain, and also because it binds and inactivates IRF3, a central regulator of interferon production. An important question remains the role of N(pro) in the inhibition of apoptosis. In this study, apoptotic signals induced by staurosporine, interferon, double stranded RNA, sodium arsenate and hydrogen peroxide were inhibited by expression of wild type N(pro), but not by mutant protein N(pro) C112R, which we show is less efficient at promoting degradation of IRF3, and led to the conclusion that N(pro) inhibits the stress-induced intrinsic mitochondrial pathway through inhibition of IRF3-dependent Bax activation. Both expression of N(pro) and infection with Bovine Viral Diarrhea Virus (BVDV) prevented Bax redistribution and mitochondrial fragmentation. Given the role played by signaling platforms during IRF3 activation, we have studied the subcellular distribution of N(pro) and we show that, in common with many other viral proteins, N(pro) targets mitochondria to inhibit apoptosis in response to cell stress. N(pro) itself not only relocated to mitochondria but in addition, both N(pro) and IRF3 associated with peroxisomes, with over 85% of N(pro) puncta co-distributing with PMP70, a marker for peroxisomes. In addition, peroxisomes containing N(pro) and IRF3 associated with ubiquitin. IRF3 was degraded, whereas N(pro) accumulated in response to cell stress. These results implicate mitochondria and peroxisomes as new sites for IRF3 regulation by N(pro), and highlight the role of these organelles in the anti-viral pathway

The neurochemistry of therapeutics: Levodopa pharmacodynamics in Parkinson's disease

No abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34884/1/62_ftp.pd

Front-end electronics for the ALICE TPC-detector

The Front-End electronics for the Time Projection Chamber (TPC) for the ALICE experiment consists of 5x105 channels. A single readout channel is comprised of three basic units: a charge sensitive amplifier/shaper with a fast tail cancellation; a 10 bit 10 Msamples/sec low power ADC; a digital ASIC which contains the zero suppression circuit and a multiple-event buffer. Data from a number of channels (4096) are multiplexed into an optical link (DDL) by means of a local custom bus which can support a data throughput of 2 Mbyte/event at a trigger rate of 50 Hz. The construction of a prototype of this electronics is presented in this paper

XMM-Newton observations of the Small Magellanic Cloud: Be/X-ray binary pulsars active between October 2006 and June 2007

(abridged) We analysed eight XMM-Newton observations toward the Small Magellanic Cloud (SMC), performed between October 2006 and June 2007, to investigate high mass X-ray binary systems. We found new X-ray binary pulsars with periods of 202 s (XMMU J005929.0-723703), 342 s (XMMU J005403.8-722632), 645 s (XMMU J005535.2-722906) and 325 s (XMMU J005252.1-721715), in the latter case confirming the independent discovery in Chandra data. In addition we detected sixteen known Be/X-ray binary pulsars and six ROSAT-classified candidate high mass X-ray binaries. From one of the candidates, RX J0058.2-7231, we discovered X-ray pulsations with a period of 291 s which makes it the likely counterpart of XTE J0051-727. From the known pulsars, we revise the pulse period of CXOU J010206.6-714115 to 967 s, and we detected the 18.37 s pulsar XTE J0055-727 (= XMM J004911.4-724939) in outburst, which allowed us to localise the source. The pulse profiles of the X-ray pulsars show a large variety of shapes from smooth to highly structured patterns and differing energy dependence. For all the candidate high mass X-ray binaries optical counterparts can be identified with magnitudes and colours consistent with Be stars. Twenty of the Be/X-ray binaries were detected with X-ray luminosities in the range 1.5x10^35 erg/s - 5.5x10^36 erg/s. The majority of the spectra is well represented by an absorbed power-law with an average power-law index of 0.93. The absorption (in addition to the Galactic foreground value) varies over a wide range between a few 10^20 H cm^-2 and several 10^22 H cm^-2. An overall correlation of the absorption with the total SMC HI column density suggests that the absorption seen in the X-ray spectra is often largely caused by interstellar gas.Comment: Accepted for publication in A&A, 22 pages, 12 figure

Host Factors interacting with the Pestivirus N terminal protease, Npro are Components of the Ribonucleoprotein Complex

The viral N-terminal protease N(pro) of pestiviruses counteracts cellular antiviral defenses through inhibition of IRF3. Here we used mass spectrometry to identify a new role for N(pro) through its interaction with over 55 associated proteins, mainly ribosomal proteins and ribonucleoproteins, including RNA helicase A (DHX9), Y-box binding protein (YBX1), DDX3, DDX5, eIF3, IGF2BP1, multiple myeloma tumor protein 2, interleukin enhancer binding factor 3 (IEBP3), guanine nucleotide binding protein 3, and polyadenylate-binding protein 1 (PABP-1). These are components of the translation machinery, ribonucleoprotein particles (RNPs), and stress granules. Significantly, we found that stress granule formation was inhibited in MDBK cells infected with a noncytopathic bovine viral diarrhea virus (BVDV) strain, Kyle. However, ribonucleoproteins binding to N(pro) did not inhibit these proteins from aggregating into stress granules. N(pro) interacted with YBX1 though its TRASH domain, since the mutant C112R protein with an inactive TRASH domain no longer redistributed to stress granules. Interestingly, RNA helicase A and La autoantigen relocated from a nuclear location to form cytoplasmic granules with N(pro). To address a proviral role for N(pro) in RNP granules, we investigated whether N(pro) affected RNA interference (RNAi), since interacting proteins are involved in RISC function during RNA silencing. Using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) silencing with small interfering RNAs (siRNAs) followed by Northern blotting of GAPDH, expression of N(pro) had no effect on RNAi silencing activity, contrasting with other viral suppressors of interferon. We propose that N(pro) is involved with virus RNA translation in the cytoplasm for virus particle production, and when translation is inhibited following stress, it redistributes to the replication complex. IMPORTANCE Although the pestivirus N-terminal protease, N(pro), has been shown to have an important role in degrading IRF3 to prevent apoptosis and interferon production during infection, the function of this unique viral protease in the pestivirus life cycle remains to be elucidated. We used proteomic mass spectrometry to identify novel interacting proteins and have shown that N(pro) is present in ribosomal and ribonucleoprotein particles (RNPs), indicating a translational role in virus particle production. The virus itself can prevent stress granule assembly from these complexes, but this inhibition is not due to N(pro). A proviral role to subvert RNA silencing through binding of these host RNP proteins was not identified for this viral suppressor of interferon