51 research outputs found
Intracellular Trafficking of Guanylate-Binding Proteins Is Regulated by Heterodimerization in a Hierarchical Manner
Guanylate-binding proteins (GBPs) belong to the dynamin family of large GTPases and represent the major IFN-γ-induced proteins. Here we systematically investigated the mechanisms regulating the subcellular localization of GBPs. Three GBPs (GBP-1, GBP-2 and GBP-5) carry a C-terminal CaaX-prenylation signal, which is typical for small GTPases of the Ras family, and increases the membrane affinity of proteins. In this study, we demonstrated that GBP-1, GBP-2 and GBP-5 are prenylated in vivo and that prenylation is required for the membrane association of GBP-1, GBP-2 and GBP-5. Using co-immunoprecipitation, yeast-two-hybrid analysis and fluorescence complementation assays, we showed for the first time that GBPs are able to homodimerize in vivo and that the membrane association of GBPs is regulated by dimerization similarly to dynamin. Interestingly, GBPs could also heterodimerize. This resulted in hierarchical positioning effects on the intracellular localization of the proteins. Specifically, GBP-1 recruited GBP-5 and GBP-2 into its own cellular compartment and GBP-5 repositioned GBP-2. In addition, GBP-1, GBP-2 and GBP-5 were able to redirect non-prenylated GBPs to their compartment in a prenylation-dependent manner. Overall, these findings prove in vivo the ability of GBPs to dimerize, indicate that heterodimerization regulates sub-cellular localization of GBPs and underscore putative membrane-associated functions of this family of proteins
Health and Sport Sciences 50
Gathering before new student convocation.https://digitalcommons.otterbein.edu/health_sports_images_all/1176/thumbnail.jp
Health and Sport Sciences 02
HSS 2018-19 Faculty/Staffhttps://digitalcommons.otterbein.edu/health_sports_images_all/1008/thumbnail.jp
Public Health Education 32
Inaugural initiation of Eta Sigma Gamma Public Health Honorary.https://digitalcommons.otterbein.edu/health_sports_images_all/1172/thumbnail.jp
Public Health Education 31
Inaugural initiation of Eta Sigma Gamma Public Health Honorary.https://digitalcommons.otterbein.edu/health_sports_images_all/1171/thumbnail.jp
Public Health Education 30
Inaugural initiation of Eta Sigma Gamma Public Health Honorary.https://digitalcommons.otterbein.edu/health_sports_images_all/1170/thumbnail.jp
HSS general 112
Dr. Kristy McCray New Teacher Award 2017https://digitalcommons.otterbein.edu/health_sports_images_all/1384/thumbnail.jp
Allied Health 11
Allied Health major Meggin Kelley addresses guests at the Stand campaign celebration donor dinner held at The Point. Meggin\u27s speech was about her Otterbein experience relative to the impact of donor gifts.https://digitalcommons.otterbein.edu/health_sports_images_all/1025/thumbnail.jp
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