Isolation, Characterization and Activity Test of Soil Origin Bacteria Amilage

This study aimed to obtain amylase-producing potent bacteria from soil and test the amylase activity produced. Soil samples were taken from the Biological Education and Research Forest, Andalas University. The isolation was done by using the stratified dilution technique on agar media. The screening of amylase activity employed the qualitative and quantitative tests on agar starch. From 8 isolated amylolytic bacteria, there were three isolates with amylolytic potential. The results of characterization and identification based on Bergey's Manual of Determinative Bacteriology show that isolates A4, A1, and A6 belonged to the genus Bacillus, Corynebacterium, and Klebsiella. The bacteria obtained can then be produced and optimized for the needs of industrial enzymes

Gleichenia linearis (Burm.) C. B. Clarke Leaves Extract Potent as a Medicinal Plant Based on Its Phytochemical Profile and The Total Phenolic Content

Resam fern (Gleichenia linearis (Burm.) C.B. Clarke) is a terrestrial plant that is easy to grow and has been used for medicinal purposes as by the community empirically. This study aimed to explore the potential of Gleichenia linearis (Burm.) C.B. Clarke) as medicinal plants based on their phytochemical profiles and total phenol content of the extract. Gleichenia linearis (Burm.) C.B. Clarke leaves were extracted with aquadest and methanol as a solvent by maceration method. Phytochemical content was detected qualitatively and the total phenol content was determined using colorimetric method with Folin-Ciocalteu reagent. As the result, qualitative screening for secondary metabolic contents in Gleichenia linearis (Burm.) C.B. Clarke extract found that the aqueous extract contains alkaloids, phenols, flavonoids, tannins, triterpenoids and steroids, but the saponin and steroid were not detected. In the methanol extract, all secondary metabolites were detected except triterpenoids. The total phenol content of methanol extract was higher than that of aqueous extract with levels of 127,08 mg/g GAE and 42,32 mg/g GAE, respectively. Based on these findings, it can be concluded that the Gleichenia linearis (Burm.) C.B. Clarke leaf has the potential to be developed as a medicinal plant

Isolasi Bakteri Pendegradasi Limbah Cair Industri Minyak Sawit

An isolation of degrading bacteria of Palm Oil Mill Effluent (POME) was conducted from May to October 2014 at the Laboratory of Microbiology, Department of Biology, Faculty of Natural Sciences, Andalas University. This study aimed to describe the characteristic of the bacteria which can degradate the POME. The result obtained five isolates ie BLS1, BLS2, BLS3, BLS4 and BLS5 and their characters were rod-shaped (basil), gram-positive, motile, positive catalase and forming endospores.                                                                                                        Keywords:       isolation, characterization, degrading bacteria, POM

Uji Organoleptik dan Umur Simpan Soyghurt dengan Berbagai Konsentrasi Gula dan Waktu Inkubasi

One of fermented soybean (Glycine max L. Merr) products is soy milk, called as Thew Fu Sui by the community of Bangka Belitung. Soy milk product has unpleasant aroma or “langu” and need to be processed into alternative product such as yogurt. Soy milk yogurt (soyghurt) was made by utilizing the same bacteria in yogurt-making from animal milk including Streptococcus thermophillus and Lactobacillus bulgaricus. Sugar concentration and incubation time were the factors that influenced the fermentation process and product’s taste. This research was conducted to determine the level of people's preference for soyghurt by organoleptic assessment of color, taste, aroma and texture based on variation of sugar concentration and incubation time of soyghurt. Our  study found that various additional sugar concentration and incubation time gave difference result in organoleptic test. Soyghurt with 10%, 12.5%, and 15% of sugar addition became more favorable among the participant. The incubation time of 8 hours, 12 hours, and 16 hours equally produced good fermentation results. Storage in refrigerator for 1 days could maintain its flavor, texture, colour and aroma, but changed after 2 days stored

Isolasi dan Karakterisasi Bakteri Potensi Pendegradasi Limbah Cair Industri Minyak Sawit Di PT. Sumbar Andals Kencana, Kab. Dharmasraya, Sumatera Barat.

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ISOLASI DAN KARAKTERISASI BAKTERI POTENSIF PENDEGRADASI LIMBAH CAIR INDUSTRI MINYAK SAWIT DI PT. SUMBAR ANDALAS KENCANA, KAB. DHARMASRAYA, SUMATERA BARAT

Penelitian tentang Isolasi dan Karakterisasi Bakteri Potensif Pendegradasi Limbah Cair Industri Minyak Sawit di PT. Sumbar Andalas Kencana, Kab. Dharmasraya, Sumatera Barat telah dilaksanakan dari bulan Mei sampai Oktober 2014, di Laboratorium Riset Mikrobiologi dan Laboratorium Pendidikan III, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Penelitian ini menggunakan metoda survey dengan tiga tahapan yaitu isolasi, karakterisasi dan pengujian bakteri potensif secara in vitro dan dianalisis secara deskriptif. Dari hasil penelitian diperoleh 51,3% (2,0x103 cfu/ml) keberadaan bakteri yang berpotensi mendegradasi limbah cair industri minyak sawit. Lima isolat yang ditemukan (BLS1, BLS2, BLS3, BLS4 dan BLS5) berbentuk batang (basil), Gram-positif, motil, katalase positif dan mempunyai endospora. Isolat BLS1 merupakan isolat yang paling potensif dan memiliki nilai indeks-lipolitik (2.33), -proteolitik (2.14), -amilolitik (2.00) dan -selulolitik (2.00) secara in vitro