Automating the determination of wave speed using the pu-loop method

The PU-loop (pressure-velocity loop) is a method for determining wave speed and relies on the linear relationship between the pressure and velocity in the absence of reflected waves. This linearity of the PU-loop during early systole, which is directly related to wave speed, has always been established by eye. This paper presents a new technique that establishes this linearity and thus determining wave speed online. Pressure and flow were measured in the ascending aorta of 11 anesthetised dogs. The slope of the PU-loop, indicating wave speed was determined by eye and by using the new technique. The difference between the slopes of the two methods is in the order of 3%. The new technique is convenient and allows for the online assessment of wave speed, which could be used as a bedside tool for the assessment of arterial compliance

Study of a bi-directional axial flow blood pump

This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.A common treatment for circulatory disorders is the application of rotary blood pumps to locally increase blood flow to required levels. Existing devices tend to support flow from inlet to outlet and in that direction only. This thesis presents a bi-directional pump that may enable ventricle assist devices (VAD) to support blood flow to the organs during systole, when rotating in one direction, and to increase coronary perfusion during diastole, when rotating in the other. For each flow direction blade profiles were designed and tested for performance. Both designs were merged to obtain a symmetric profile to provide flow support in both directions. This initial bi-directional design was optimised using computational fluid dynamics modelling. The model was set to accelerate to a maximum forward rotational speed of 8,000 rpm, change rotational direction after 300 ms and accelerate to 2,400 rpm whilst rotating backwards. Experimental testing was carried out to validate the computational results. In the forward direction, the pump was predicted to deliver 39 cm3 compared to 19 cm3 in the backward direction. Pressure heads reached maxima of 2.2 kPa in forward and 0.16 kPa in backward direction. Analysis of wall shear stress profiles at the blades’ surface showed that the maximum was 140 Pa lasting less than 300 ms in the forward direction, whilst in the backward direction this was approximately 23 Pa lasting for 700 ms. A design for the bi-directional blades is established and characterised computationally and experimentally. Analysis of the blade pressure profiles confirmed generation of pressure rise in both directions. The computational results for wall shear stress were predicted to be below the accepted limits of haemolysis. Recirculation zones were found at the outlet in the backward rotating direction. Future work may reduce those by using guide vanes at either side of the rotor

Simultaneous determination of wave speed and arrival time of reflected waves using the pressure-velocity loop

This is the post print version of the article. The official published version can be found at the link below.In a previous paper we demonstrated that the linear portion of the pressure–velocity loop (PU-loop) corresponding to early systole could be used to calculate the local wave speed. In this paper we extend this work to show that determination of the time at which the PU-loop first deviates from linearity provides a convenient way to determine the arrival time of reflected waves (Tr). We also present a new technique using the PU-loop that allows for the determination of wave speed and Tr simultaneously. We measured pressure and flow in elastic tubes of different diameters, where a strong reflection site existed at known distances away form the measurement site. We also measured pressure and flow in the ascending aorta of 11 anaesthetised dogs where a strong reflection site was produced through total arterial occlusion at four different sites. Wave speed was determined from the initial slope of the PU-loop and Tr was determined using a new algorithm that detects the sampling point at which the initial linear part of the PU-loop deviates from linearity. The results of the new technique for detecting Tr were comparable to those determined using the foot-to-foot and wave intensity analysis methods. In elastic tubes Tr detected using the new algorithm was almost identical to that detected using wave intensity analysis and foot-to-foot methods with a maximum difference of 2%. Tr detected using the PU-loop in vivo highly correlated with that detected using wave intensity analysis (r 2 = 0.83, P < 0.001). We conclude that the new technique described in this paper offers a convenient and objective method for detecting Tr, and allows for the dynamic determination of wave speed and Tr, simultaneously

Preparation and characterization of an anionic dye-polycation molecular films by electrostatic Layer-by-Layer adsorption process

This communication reports the formation and characterization of self assembled films of a low molecular weight anionic dye amaranth and polycation Poly (allylamine hydrochloride) (PAH) by electrostatic alternating Layer-by-Layer (LBL) adsorption. It was observed that there was almost no material loss occurred during adsorption process. The UV-Vis absorption and fluorescence spectra of amaranth solution reveal that with the increase in amaranth concentration in solution, the aggregated species starts to dominate over the monomeric species. New aggregated band at 600 nm was observed in amaranth-PAH mixture solution absorption spectrum. A new broad low intense band at the longer wavelength region, in the amaranth-PAH mixture solution fluorescence spectrum was observed due to the closer association of amaranth molecule while tagged into the polymer backbone of PAH and consequent formation of aggregates. The broad band system in the 650-750 nm region in the fluorescence spectra of different layered LBL films changes in intensity distribution among various bands within itself, with changing layer number and at 10 bilayer LBL films the longer wavelength band at 710 nm becomes prominent. Existence of dimeric or higher order n-meric species in the LBL films was confirmed by excitation spectroscopic studies. Almost 45 minute was required to complete the interaction between amaranth and PAH molecules in the 1-bilayer LBL film.Comment: 10 pages, 5 figure

Efficient and broadband optical parametric four wave mixing in chalcogenide-PMMA hybrid microwires

The recent development of devices based on novel nonlinear materials like chalcogenides (ChGs), silicon (Si) and other semi-conductors has revolutionized the field of nonlinear photonics [1,2,3]. Among the nonlinear effects observed in these materials, four-wave mixing (FWM) is the process that finds the most applications including wavelength conversion [4], optical regeneration [5,6], optical delay [7], time-domain demultiplexing[8], temporal cloaking[9] and negative refraction[10]. Although FWM has been observed in several media including chalcogenides [11,12,13,14], silicon[15, 16], bismuth [17] and silica [18,19], there is a continued quest for devices that realize efficient and broadband FWM while offering compactness, low-power consumption and compatibility with optical fibers. Here, we demonstrate the fabrication of 10 cm long polymer cladded chalcogenide (As2Se3) microwires to realize FWM-led sub watt threshold (70-370 mW) wavelength conversion with a 12 dB bandwidth as broad as 190 nm, and conversion efficiency as high as 21 dB. This represents a 3-30 \times increase in bandwidth and 30-50 dB improvement in conversion efficiency over previous demonstrations in tapered and microstructured chalcogenide fibers [11,13,14]. These properties, combined with low loss (< 4 dB), ease of fabrication, and the transparency of As2Se3 from near to mid infrared regions (1 15 \mum) [20] make this device a promising building block for lasers, optical instrumentation and optical communication devices

A Mock Circulatory System Incorporating a Compliant 3D-Printed Anatomical Model to Investigate Pulmonary Hemodynamics

A realistic mock circulatory system (MCS) could be a valuable in vitro testbed to study human circulatory hemodynamics. The objective of this study was to design a MCS replicating the pulmonary arterial circulation, incorporating an anatomically representative arterial model suitable for testing clinically relevant scenarios. A second objective of the study was to ensure the system's compatibility with magnetic resonance imaging (MRI) for additional measurements. A latex pulmonary arterial model with two generations of bifurcations was manufactured starting from a 3D-printed mold reconstructed from patient data. The model was incorporated into a MCS for in vitro hydrodynamic measurements. The setup was tested under physiological pulsatile flow conditions and results were evaluated using wave intensity analysis (WIA) to investigate waves traveling in the arterial system. Increased pulmonary vascular resistance (IPVR) was simulated as an example of one pathological scenario. Flow split between right and left pulmonary artery was found to be realistic (54 and 46%, respectively). No substantial difference in pressure waveform was observed throughout the various generations of bifurcations. Based on WIA, three main waves were identified in the main pulmonary artery (MPA), that is, forward compression wave, backward compression wave, and forward expansion wave. For IPVR, a rise in mean pressure was recorded in the MPA, within the clinical range of pulmonary arterial hypertension. The feasibility of using the MCS in the MRI scanner was demonstrated with the MCS running 2 h consecutively while acquiring preliminary MRI data. This study shows the development and verification of a pulmonary MCS, including an anatomically correct, compliant latex phantom. The setup can be useful to explore a wide range of hemodynamic questions, including the development of patient- and pathology-specific models, considering the ease and low cost of producing rapid prototyping molds, and the versatility of the setup for invasive and noninvasive (i.e., MRI) measurements

Plasmonic DNA: Towards Genetic Diagnosis Chips

"Plasmonics and DNA" special issueInternational audienc