Ultrasensitivity in phosphorylation-dephosphorylation cycles with little substrate

Cellular decision-making is driven by dynamic behaviours, such as the preparations for sunrise enabled by circadian rhythms and the choice of cell fates enabled by positive feedback. Such behaviours are often built upon ultrasensitive responses where a linear change in input generates a sigmoidal change in output. Phosphorylation-dephosphorylation cycles are one means to generate ultrasensitivity. Using bioinformatics, we show that in vivo levels of kinases and phosphatases frequently exceed the levels of their corresponding substrates in budding yeast. This result is in contrast to the conditions often required by zero-order ultrasensitivity, perhaps the most well known means for how such cycles become ultrasensitive. We therefore introduce a mechanism to generate ultrasensitivity when numbers of enzymes are higher than numbers of substrates. Our model combines distributive and non-distributive actions of the enzymes with two-stage binding and concerted allosteric transitions of the substrate. We use analytical and numerical methods to calculate the Hill number of the response. For a substrate with [Formula: see text] phosphosites, we find an upper bound of the Hill number of [Formula: see text], and so even systems with a single phosphosite can be ultrasensitive. Two-stage binding, where an enzyme must first bind to a binding site on the substrate before it can access the substrate's phosphosites, allows the enzymes to sequester the substrate. Such sequestration combined with competition for each phosphosite provides an intuitive explanation for the sigmoidal shifts in levels of phosphorylated substrate. Additionally, we find cases for which the response is not monotonic, but shows instead a peak at intermediate levels of input. Given its generality, we expect the mechanism described by our model to often underlay decision-making circuits in eukaryotic cells

Spherical solid-propellant rocket motor Patent

Spherical solid propellant rocket engine desig

The decision tree approach to classification

A class of multistage decision tree classifiers is proposed and studied relative to the classification of multispectral remotely sensed data. The decision tree classifiers are shown to have the potential for improving both the classification accuracy and the computation efficiency. Dimensionality in pattern recognition is discussed and two theorems on the lower bound of logic computation for multiclass classification are derived. The automatic or optimization approach is emphasized. Experimental results on real data are reported, which clearly demonstrate the usefulness of decision tree classifiers

Ground-based NIR emission spectroscopy of HD189733b

We investigate the K and L band dayside emission of the hot-Jupiter HD 189733b with three nights of secondary eclipse data obtained with the SpeX instrument on the NASA IRTF. The observations for each of these three nights use equivalent instrument settings and the data from one of the nights has previously reported by Swain et al (2010). We describe an improved data analysis method that, in conjunction with the multi-night data set, allows increased spectral resolution (R~175) leading to high-confidence identification of spectral features. We confirm the previously reported strong emission at ~3.3 microns and, by assuming a 5% vibrational temperature excess for methane, we show that non-LTE emission from the methane nu3 branch is a physically plausible source of this emission. We consider two possible energy sources that could power non-LTE emission and additional modelling is needed to obtain a detailed understanding of the physics of the emission mechanism. The validity of the data analysis method and the presence of strong 3.3 microns emission is independently confirmed by simultaneous, long-slit, L band spectroscopy of HD 189733b and a comparison star.Comment: ApJ accepte

The mid-infrared spectrum of the transiting exoplanet HD 209458b

We report the spectroscopic detection of mid-infrared emission from the transiting exoplanet HD 209458b. Using archive data taken with the Spitzer/IRS instrument, we have determined the spectrum of HD 209458b between 7.46 and 15.25 microns. We have used two independent methods to determine the planet spectrum, one differential in wavelength and one absolute, and find the results are in good agreement. Over much of this spectral range, the planet spectrum is consistent with featureless thermal emission. Between 7.5 and 8.5 microns, we find evidence for an unidentified spectral feature. If this spectral modulation is due to absorption, it implies that the dayside vertical temperature profile of the planetary atmosphere is not entirely isothermal. Using the IRS data, we have determined the broad-band eclipse depth to be 0.00315 +/- 0.000315, implying significant redistribution of heat from the dayside to the nightside. This work required development of improved methods for Spitzer/IRS data calibration that increase the achievable absolute calibration precision and dynamic range for observations of bright point sources.Comment: 35 pages, 12 figures, revised version accepted by the Astrophysical Journa

Layered classification techniques for remote sensing applications

The layered classifier method is outlined and several applications to pattern classification for which the approach is suited are discussed

Hepatitis B immunisation : a survey of surgeons and theatre nurses

Acute viral hepatitis remains a serious condition. Its long-term sequelae include cirrhosis and hepatocellular carcinoma. Health workers constitute a high-risk group for contracting hepatitis B. A group of clinicians associated with invasive procedures and nurses working in operating theatres were invited to answer a confidential questionnaire concerning hepatitis B immunisation and the use of protective measures. A self-administered confidential questionnaire was sent to 152 clinicians and 97 theatre nurses, of whom 82 and 74 respectively responded, giving an overall response rate of 63 %. Whilst 91% of respondents considered their speciality as being of high risk for hepatitis B only 63% of them were fully immunised and of these only 51% had had their immunity tested. Out of those who checked their antibody status 19% did so following a needle stick injury. 60% of our respondents had had a needle stick injury over the past year. Even so barrier precaution techniques were used infrequently with only 17% always or at least frequently using double gloving and 10% wearing a visor during operations. Some respondents also commented on the poor availability of resources such as impermeable gowns or blunt needles which are established precautions against contamination from hepatitis B.peer-reviewe

Brca2 and Trp53 deficiency cooperate in the progression of mouse prostate tumourigenesis.

Epidemiological studies have shown that one of the strongest risk factors for prostate cancer is a family history of the disease, suggesting that inherited factors play a major role in prostate cancer susceptibility. Germline mutations in BRCA2 predispose to breast and ovarian cancer with its predominant tumour suppressor function thought to be the repair of DNA double-strand breaks. BRCA2 has also been implicated in prostate cancer etiology, but it is unclear the impact that mutations in this gene have on prostate tumourigenesis. Here we have undertaken a genetic analysis in the mouse to determine the role of Brca2 in the adult prostate. We show that deletion of Brca2 specifically in prostate epithelia results in focal hyperplasia and low-grade prostate intraepithelial neoplasia (PIN) in animals over 12 months of age. Simultaneous deletion of Brca2 and the tumour suppressor Trp53 in prostate epithelia gave rise to focal hyperplasia and atypical cells at 6 months, leading to high-grade PIN in animals from 12 months. Epithelial cells in these lesions show an increase in DNA damage and have higher levels of proliferation, but also elevated apoptosis. Castration of Brca2;Trp53 mutant animals led to regression of PIN lesions, but atypical cells persisted that continued to proliferate and express nuclear androgen receptor. This study provides evidence that Brca2 can act as a tumour suppressor in the prostate, and the model we describe should prove useful in the development of new therapeutic approaches

Contextual classification of multispectral image data

There are no author-identified significant results in this report

Vision-based weed identification with farm robots

Robots in agriculture offer new opportunities for real time weed identification and quick removal operations. Weed identification and control remains one of the most challenging task in agriculture, particularly in organic agriculture practices. Considering environmental impacts and food quality, the excess use of chemicals in agriculture for controlling weeds and diseases is decreasing. The cost of herbercides and their field applications must be optimized. As an alternative, a smart weed identification technique followed by the mechanical and thermal weed control can fulfill the organic farmers’ expectations. The smart identification technique works on the concept of ‘shape matching’ and ‘active shape modeling’ of plant and weed leafs. The automated weed detection and control system consists of three major tools. Such as: i) eXcite multispectral camera, ii) LTI image processing library and iii) Hortibot robotic vehicle. The components are combined in Linux interface environment in the eXcite camera associate PC. The laboratory experiments for active shape matching have shown interesting results which will be further enhanced to develop the automated weed detection system. The Hortibot robot will be mounted with the camera unit in the front-end and the mechanical weed remover in the rear-end. The system will be upgraded for intense commercial applications in maize and other row crops