Is Someone Reading \u3cem\u3eUs\u3c/em\u3e? Fourth Grade Students Respond to Postmodern Picture Books

The purpose of this naturalistic case study was to gain understanding about the comprehension strategies successful readers employ as they construct meaning while navigating through postmodem picture books. Participants were eight fourth grade students from a large suburban elementary school in Tennessee. Data included transcripts and field notes from ten individual think aloud sessions and five group book club discussions. Themes identified from the think aloud sessions related to the ways in which students navigated through postmodem picture books include: emotional responses, general story problem solving and postmodem story problem solving. Themes identified from the group book club discussions related to the ways in which students navigated through postmodem picture books include: aesthetic responses, reflecting on reading behaviors, general story problem solving, and postmodern story problem solving. A general linear navigation pattern was identified from the think aloud session transcripts which involved encountering metafictive elements, emotional responses and problem solving. Book club discussions, on the other hand, produced a more interactive, dynamic navigation pattern in which participants shared aesthetic responses, reflected on / their own reading behaviors, and spent time problem solving within the postmodem picture book story world. Data revealed that the fourth grade students in this study came to further their understanding of each postmodem picture book through the group book club discussions that followed their individual readings. The choice to explore the phenomenon in two ways, individual think aloud sessions and group book club discussions, proved to be integral in providing a rich description of participants\u27 experience with postmodern picture books. Literacy in the 21st century means thinking critically, making sense of a bombardment of media sources, negotiating multiple digital literacies, and making choices about what to read and how to go about reading it. The findings from this study indicate that postmodem picture books have great potential to nurture growth in these areas if thoughtfully integrated into the curriculum. With the insight gained from this study, teachers should be mindful to support and encourage students who might become frustrated as a result of more negative emotional and/or aesthetic responses to postmodem picture books. They can work to facilitate a classroom environment where students feel safe in their comments, questions, and responses during discussions about literature. The classroom environment should be conceptualized as a place where this sort of active engagement is valued and encouraged. Data from this study revealed the complexity of the ways in which children independently constructed meaning while navigating through postmodem picture books. It is recommended that further studies are conducted in order to provide insight about how students might be supported toward more positive engagements with this emerging genre

Use of Research Aircraft Data to Validate Mesoscale Model Forecasts

A NSF funded Student Training in Airborne Research and Technology (START) two-week deployment of the University of Wyoming King Air (UWKA) research aircraft was conducted at Embry-Riddle Aeronautical University (ERAU) in Prescott, Arizona during late March and early April 2014. Some of the goals of this program were to build knowledge on airborne atmospheric research for undergraduate students across multiple departments and to collect a valuable set of aircraft data for atmospheric model validation. Data collection for 10 research flights is available for mesoscale model case study validation. This project utilizes the Weather Research and Forecasting mesoscale model (WRF), version 3.6.1 Advanced Research WRF (ARW) to simulate the general features of the boundary layer thermodynamic profiles, winds and cloud structure prior to and during the days of selected research flights. Data assimilation and two-way nesting procedures are executed. A fine-grid resolution used for this study is 10 km, while the coarse grid resolution is 30 km. This study investigates case studies of cross-wind and cloud microphysical conditions which limit ERAU pilot training operations, and provide insight on the potential value of implementing the WRF model at ERAU with specialized forecast products that support the ERAU pilot training program

Bridging the Gap Between Researchers and Wider Audiences: Navigating A Community Literacy Collaboration in Real-Time

While campus-community partnerships are common, such engaged scholarship efforts often lead university researchers to community-centered presentations and publications; however, this type of scholarship and especially the venues in which it is often disseminated are of questionable value within the academy’s tenure and promotion process. Three literacy scholars who were invited to study the impact of community-wide Imagination Library implementation share challenges they encountered related to collaboration, communication, and dissemination of findings during the first two years of a five-year early literacy community partnership. Selected outcomes and implications for other community-engaged scholars include (a) investing in true multi-directional consistent collaboration and communication and (b) leveraging user-friendly technology tools and platforms to archive and share project work. The authors call for continued efforts to communicate beyond the academy with the audiences targeted for ultimate impact and continued advocacy for valuing non-traditional publications within the academy

Global Dissemination of Scholarly Research through Open Source System

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Comparison of SIVmac239(352–382) and SIVsmmPBj41(360–390) enterotoxic synthetic peptides

AbstractTo characterize the active domain of the simian immunodeficiency virus (SIV) surface unit (SU) enterotoxin, peptides corresponding to the V3 loop of SIVmac239 (SIVmac) and SIVsmmPBj41 (SIVpbj) were synthesized and examined for enterotoxic activity, α-helical structure, and interaction(s) with model membranes. SIVmac and SIVpbj induced a dose-dependent diarrhea in 6–8-day-old mouse pups similar to full-length SU. The peptides mobilized [Ca2+]i in HT-29 cells with distinct oscillations and elevated inositol triphosphate levels. Circular dichroism analyses showed the peptides were predominantly random coil in buffer, but increased in α-helical content when placed in a hydrophobic environment or with cholesterol-containing membrane vesicles that are rich in anionic phospholipids. None of the peptides underwent significant secondary structural changes in the presence of neutral vesicles indicating ionic interactions were important. These data show that the SIV SU enterotoxic domain localizes in part to the V3 loop region and interacts with anionic membrane domains on the host cell surface

A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

During the first week after hatch, young chicks are vulnerable to pathogens as the immune system is not fully developed. The objectives of this study were to determine if supplementing the starter diet with a microencapsulated feed additive containing citric and sorbic acids, thymol, and vanillin affects in vitro functional activity of peripheral blood leukocytes (PBLs). Day-old chicks (n = 800) were assigned to either a control diet (0 g/metric ton [MT]) or a diet supplemented with 500 g/MT of the microencapsulated additive. At 4 D of age, peripheral blood was collected (100 birds per treatment), and heterophils and monocytes isolated (n = 4). Heterophils were assayed for the ability to undergo degranulation and production of an oxidative burst response while nitric oxide production was measured in monocytes. Select cytokine and chemokine mRNA expression levels were also determined. Statistical analysis was performed using Student t test comparing the supplemented diet to the control (P ≤ 0.05). Heterophils isolated from chicks fed the microencapsulated citric and sorbic acids, thymol, and vanillin had higher (P ≤ 0.05) levels of degranulation and oxidative burst responses than those isolated from chicks on the control diet. Heterophils from the supplemented chicks also had greater (P ≤ 0.05) expression of IL10, IL1β, and CXCL8 mRNA than those from control-fed chicks. Similarly, nitric oxide production was significantly (P ≤ 0.05) higher in monocytes isolated from birds fed the supplement. The cytokine and chemokine profile in monocytes from the supplement-fed chicks showed a significant (P ≤ 0.05) drop in IL10 mRNA expression while IL1β, IL4, and CXCL8 were unchanged. In conclusion, 4 D of supplementation with a microencapsulated blend made up of citric and sorbic acids, thymol, and vanillin enhanced the in vitro PBL functions of degranulation, oxidative burst, and nitric oxide production compared with the control diet. Collectively, the data suggest feeding broiler chicks a diet supplemented with a microencapsulated blend of citric and sorbic acids, thymol, and vanillin may prime key immune cells making them more functionally efficient and acts as an immune-modulator to boost the inefficient and undeveloped immune system of young chicks

Gene Expression Profiling of the Local Cecal Response of Genetic Chicken Lines That Differ in Their Susceptibility to Campylobacter jejuni Colonization

Campylobacter jejuni (C. jejuni) is one of the most common causes of human bacterial enteritis worldwide primarily due to contaminated poultry products. Previously, we found a significant difference in C. jejuni colonization in the ceca between two genetically distinct broiler lines (Line A (resistant) has less colony than line B (susceptible) on day 7 post inoculation). We hypothesize that different mechanisms between these two genetic lines may affect their ability to resist C. jejuni colonization in chickens. The molecular mechanisms of the local host response to C. jejuni colonization in chickens have not been well understood. In the present study, to profile the cecal gene expression in the response to C. jejuni colonization and to compare differences between two lines at the molecular level, RNA of ceca from two genetic lines of chickens (A and B) were applied to a chicken whole genome microarray for a pair-comparison between inoculated (I) and non-inoculated (N) chickens within each line and between lines. Our results demonstrated that metabolism process and insulin receptor signaling pathways are key contributors to the different response to C. jejuni colonization between lines A and B. With C. jejuni inoculation, lymphocyte activation and lymphoid organ development functions are important for line A host defenses, while cell differentiation, communication and signaling pathways are important for line B. Interestingly, circadian rhythm appears play a critical role in host response of the more resistant A line to C. jejuni colonization. A dramatic differential host response was observed between these two lines of chickens. The more susceptible line B chickens responded to C. jejuni inoculation with a dramatic up-regulation in lipid, glucose, and amino acid metabolism, which is undoubtedly for use in the response to the colonization with little or no change in immune host defenses. However, in more resistant line A birds the host defense responses were characterized by an up-regulation lymphocyte activation, probably by regulatory T cells and an increased expression of the NLR recognition receptor NALP1. To our knowledge, this is the first time each of these responses has been observed in the avian response to an intestinal bacterial pathogen

Teacher educators teaching and learning together: A collaborative self-study of support within an online literacy learning community

Abstract In this self-study, five university instructors who taught the same online literacy course during a summer session formed an online learning community. Through engaging in email exchanges, participating in online discussions, posting weekly teaching reflections, and visiting one another's online courses, they sought to answer the research question: How can an online literacy learning community support university instructors who teach various sections of a common online course? In answering the research question, the researchers identified the themes that were most prominent in their discussions and reflections, and also the themes that played minor roles in the forming of the literacy learning community

A blend of microencapsulated organic acids and botanicals reduces necrotic enteritis via specific signaling pathways in broilers

Necrotic enteritis (NE) is a devastating disease that has seen a resurgence of cases following the removal of antibiotics from feed resulting in financial loss and significant animal health concerns across the poultry industry. The objective was to evaluate the efficacy of a microencapsulated blend of organic (25 % citric and 16.7% sorbic) acids and botanicals (1.7% thymol and 1% vanillin [AviPlus®P]) to reduce clinical NE and determine the signaling pathways associated with any changes. Day-of-hatch by-product broiler breeder chicks were randomly assigned to a control (0) or supplemented (500 g/MT) diet (n=23-26) and evaluated in a NE challenge model (n=3). Birds were administered 2X cocci vaccine on d14 and challenged with a cocktail of Clostridium perfringens strains (107) on d17-19. On d20-21 birds were weighed, euthanized, and scored for NE lesions. Jejunal tissue was collected for kinome analysis using an immuno-metabolism peptide array (n=5; 15/treatment) to compare tissue from supplement-fed birds to controls. Mortality and weight were analyzed using Student's t-test and lesion scores analyzed using F-test two-sample for variances (P<0.05). The kinome data was analyzed using PIIKA2 peptide array analysis software and fold-change between control and treated groups determined. Mortality in the supplemented group was 47.4% and 70.7% in controls (P=0.004). Lesions scores were lower (P=0.006) in supplemented birds (2.47) compared to controls (3.3). Supplement-fed birds tended (P=0.19) to be heavier (848.6g) than controls (796.2g). Kinome analysis showed T cell receptor, TNF and NF-kB signaling pathways contributed to the improvements seen in the supplement-fed birds. The following peptides were significant (P<0.05) in all three pathways: CHUK, MAP3K14, MAP3K7, and NFKB1 indicating their importance. Additionally, there were changes to IL6, IL10, and IFN- γ mRNA expression in tissue between control- and supplement-fed chickens. In conclusion, the addition of a microencapsulated blend of organic acids and botanicals to a broiler diet reduced the clinical signs of NE that was mediated by specific immune-related pathways

The biological effects of microencapsulated organic acids and botanicals induces tissue-specific and dose-dependent changes to the Gallus gallus microbiota

Abstract Background: Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum have not been evaluated. Microbiota diversity is linked to, but not correlated with, the efficacy of natural products; therefore, understanding the effects on the microbiota is necessary for evaluating their potential as an antibiotic alternative. Results: Ileal and jejunal segments from control and supplement-fed chickens (300 and 500g/metric ton [MT]) were subjected to alpha diversity analysis including Shannon's diversity and Pielou's Evenness. In both analytics, the diversity in the ileum was significantly decreased compared to the jejunum irrespective of treatment. Similarly, beta diversity metrics including Bray-Curtis dissimilarity index and Weighted Unifrac Distance Matrix, were significant (Q<0.05) for both tissue and treatments comparisons. Alpha and beta diversity analytics indicated compartmentalization effects between the ileum and jejunum. Additionally, analysis of communities in the microbiota (ANCOM) analysis showed Lactobacilliaceae predominated the total operational taxonomic units (OTU), with a stepwise increase from 53% in the no treatment control (NTC) to 56% in the 300g/MT and 67% in the 500g/MT group. Staphylococcaceae were 2% in NTC and 2 and 0% in 300 and 500g/MT groups. Enterobacteriaceae decreased in the 500g/MT (31%) and increased in the 300g/MT (37%) compared to the NTC (35%). Aerococcaceae was 0% for both doses and 7% in NTC. Ruminococcaceae were 0% in NTC and 2% and 1% in the 300 and 500g/MT. These changes in the microbial consortia were statistically (Q<0.05) associated with treatment groups in the jejunum that were not observed in the ileum. Least discriminant analysis effect size (LEfSE) indicated different changes directly corresponding to treatment. Enterobacteriaceae demonstrated a stepwise decrease (from NTC onward) while Clostridiaceae, were significantly increased in the 500g/MT compared to NTC and 300g/MT (P<0.05).Conclusion: The bioactive site for the microencapsulated blend of organic acids and botanicals was the jejunum, and dietary inclusion enhanced the GIT microbiota and may be a viable antibiotic alternative for the poultry industry