Ovi-protective mothers: exploring the proteomic profile of weevil (Gonipterus) egg capsules

Insects of different orders produce elaborate structures to protect their eggs from the many threats they may face from the environment and natural enemies. In the weevil genus Gonipterus, their dark, hardened egg capsule is possibly generated by a mixture of the insects' excrement and glandular substances. To test this hypothesis, this study focused on the elucidation of protein components present in the egg capsule cover and interrogated them through comparative analysis and gene expression to help infer potential functions. First, female Gonipterus sp. n. 2 reproductive and alimentary tissues were isolated to establish a reference transcriptome-derived protein database. Then, proteins from weevil frass (excrement) and egg capsule cover were identified through mass spectrometry proteomics. We found that certain egg capsule cover proteins were both exclusive and shared between frass and egg capsule cover, including those of plant origin (e.g. photosystem II protein) and others secreted by the weevil, primarily from reproductive tissue. Among them, a mucin/spidroin-like protein and novel proteins with repetitive units that likely play a structural role were identified. We have confirmed the dual origin of the egg capsule cover substance as a blend of the insects? frass and secretions. Novel proteins secreted by the weevils are key candidates for holding the egg case cover together

Ovi-protective mothers: exploring the proteomic profile of weevil (Gonipterus) egg capsules

Insects of different orders produce elaborate structures to protect their eggs from the many threats they may face from the environment and natural enemies. In the weevil genus Gonipterus, their dark, hardened egg capsule is possibly generated by a mixture of the insects' excrement and glandular substances. To test this hypothesis, this study focused on the elucidation of protein components present in the egg capsule cover and interrogated them through comparative analysis and gene expression to help infer potential functions. First, female Gonipterus sp. n. 2 reproductive and alimentary tissues were isolated to establish a reference transcriptome-derived protein database. Then, proteins from weevil frass (excrement) and egg capsule cover were identified through mass spectrometry proteomics. We found that certain egg capsule cover proteins were both exclusive and shared between frass and egg capsule cover, including those of plant origin (e.g. photosystem II protein) and others secreted by the weevil, primarily from reproductive tissue. Among them, a mucin/spidroin-like protein and novel proteins with repetitive units that likely play a structural role were identified. We have confirmed the dual origin of the egg capsule cover substance as a blend of the insects? frass and secretions. Novel proteins secreted by the weevils are key candidates for holding the egg case cover together

Transcriptomic discovery and comparative analysis of neuropeptide precursors in sea cucumbers (Holothuroidea)

This research was supported by Agricultural Research Development Agency (Public Organization), Thailand

Total Quality Management Practices and Technology Transfer in Malaysian Public University

It is widely accepted that the implementation of Total Quality Management (TQM) as a management philosophy has significantly contributed to good management practice in business organization particularly in the manufacturing and service sectors. The applications of that concepts, techniques and tools have been successfully tailored to non-profit service or government-based organization. In the context of higher education institution, it has been seriously debated by the TQM scholars and academicians on the issue of to what extent TQM can be applied and how relevant its practices are to higher education\u27s core business i.e. teaching and research. Despite having sufficient knowledge and research on TQM implementation in the scope of manufacturing practices and administration-related services, it is hard to find a research on TQM, which focuses on the scope of R&D at a university. This gap has to be filled because the management of research is a critical topic for universities worldwide. For developing countries such as Malaysia the need to have good management practice in R&D is even greater. Without effective research management, the task of becoming significant players in the global knowledge market will become harder. Thus, the first part of this paper will discuss the applicability of TQM and propose a theoretical framework or model of TQM to suit the need of R&D context. The constructs for the TQM framework are based on previous empirical studies and the evaluation criteria of world standard criteria such as MBNQA, EFQM, and QMS ISO 9000. The TQM constructs that will be proposed are leadership, strategic planning, student/stakeholder & industry focus, data & information management, staff management, process & system approach, partnership & resource and continuous improvement. The second part of the paper will discuss the performance indicators of R&D activities particularly in the context of public university. The review of International literatures stressed that the performance of R&D activities have to be measured. The current issue related to research performance at university is the level the research output that can be transferred to the stakeholders. Therefore, this study will use technology transfer framework to measure research performance such as publication, patents, royalty and Spin-off Company. Finally, this paper will conceptually develop a model that would show the relationship between the TQM practices in the area of research and the level of technology transfer

Structure and proteomic analysis of the crown-of-thorns starfish (Acanthaster sp.) radial nerve cord

The nervous system of the Asteroidea (starfish or seastar) consists of radial nerve cords (RNCs) that interconnect with a ring nerve. Despite its relative simplicity, it facilitates the movement of multiple arms and numerous tube feet, as well as regeneration of damaged limbs. Here, we investigated the RNC ultrastructure and its molecular components within the of Pacific crown-of-thorns starfish (COTS; Acanthaster sp.), a well-known coral predator that in high-density outbreaks has major ecological impacts on coral reefs. We describe the presence of an array of unique small bulbous bulbs (40–100 μm diameter) that project from the ectoneural region of the adult RNC. Each comprise large secretory-like cells and prominent cilia. In contrast, juvenile COTS and its congener Acanthaster brevispinus lack these features, both of which are non-corallivorous. Proteomic analysis of the RNC (and isolated neural bulbs) provides the first comprehensive echinoderm protein database for neural tissue, including numerous secreted proteins associated with signalling, transport and defence. The neural bulbs contained several neuropeptides (e.g., bombyxin-type, starfish myorelaxant peptide, secretogranin 7B2-like, Ap15a-like, and ApNp35) and Deleted in Malignant Brain Tumor 1-like proteins. In summary, this study provides a new insight into the novel traits of COTS, a major pest on coral reefs, and a proteomics resource that can be used to develop (bio)control strategies and understand molecular mechanisms of regeneration.journal articl

Aquaculture Breeding Enhancement: Maturation and Spawning in Sea Cucumbers Using a Recombinant Relaxin-Like Gonad-Stimulating Peptide

Wild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea cucumber reproductive processes. The neurohormone relaxin-like gonad-stimulating peptide (RGP) has been identified as the active gonad-stimulating peptide in sea stars (Asteroidea), which could also be present in other echinoderm groups. In this study, a sea cucumber RGP was identified and confirmed by phylogenetic analysis. A recombinant Holothuria scabra RGP was produced in the yeast Pichia pastoris and confirmed by mass spectrometry. To assess bioactivity, four levels of purification were tested in an in vitro germinal vesicle breakdown (GVBD) bioassay. The most pure form induced 98.56 ± 1.19% GVBD in H. scabra and 89.57 ± 1.19% GVBD in Holothuria leucospilota. Cruder levels of purification still resulted in some GVBD. Upon single injection into female H. scabra, the recombinant RGP induced head waving behavior followed by spawning within 90–170 min. Spawned oocytes were fertilized successfully, larvae settled and developed into juveniles. Our results provide a key finding for the development of a break-through new artificial breeding approach in sea cucumber aquaculture

A novel role for Teneurin C-terminal Associated Peptide (TCAP) in the regulation of cardiac activity in the Sydney rock oyster, Saccostrea glomerata

Teneurin C-terminal associated peptide (TCAP) is an ancient bioactive peptide that is highly conserved in metazoans. TCAP administration reduces cellular and behavioural stress in vertebrate and urochordate models, yet despite numerous studies in higher animals, there is limited knowledge of its role in invertebrates. In particular, there are no studies on TCAP’s effects on the heart of any metazoan, which is a critical organ in the stress response. We used the Sydney rock oyster (SRO) as an invertebrate model to investigate a potential role for sroTCAP in regulating cardiac activity, including during stress. sroTCAP is localized to the neural innervation network of the SRO heart, and suggested binding with various heart proteins related to metabolism and stress, including SOD, GAPDH and metabotropic glutamate receptor. Intramuscular injection of sroTCAP (10 pmol) significantly altered the expression of heart genes that are known to regulate remodelling processes under different conditions, and modulated several gene families responsible for stress mitigation. sroTCAP (1 and 10 pmol) was shown to cause transient bradycardia (heart rate was reduced by up to 63% and for up to 40 min post-administration), indicative of an unstressed state. In summary, this study has established a role for a TCAP in the regulation of cardiac activity through modulation of physiological and molecular components associated with energy conservation, stress and adaptation. This represents a novel function for TCAP and may have implications for higher-order metazoans

The neuropeptidome of the Crown-of-Thorns Starfish, Acanthaster planci

This study was supported by funds from the Australian federal government Department of the Environment Reef Rescue ‘Caring for Country’ program for funding the ‘The Crown-of-Thorns secretome: Towards a control technology’ project (M. R. H., S. C.). M.R.E. was supported by grants from the BBSRC (BB/M001644/1) and Leverhulme Trust (RPG-2013-351)

เซอร์เฟสพลาสมอนเรโซแนนซ์และอิเล็กโทรเคมีคัลแอฟฟินิตีไบโอเซนเซอร์

Thesis (Ph.D., Chemistry)--Prince of Songkla University, 200

Young coconut juice accelerates cutaneous wound healing by downregulating macrophage migration inhibitory factor (MIF) in ovariectomized rats: Preliminary novel findings

Estrogens play a crucial role in cutaneous wound healing by down-regulating macrophage migration inhibitory factor (MIF). We had previously reported the effect of young coconut juice (YCJ) known to contain the phytoestrogen, -sitosterol, on cutaneous wound healing in ovariectomized (ovx) rats. This research investigated the possible mechanisms of YCJ on cutaneous wound healing and it was found that it down regulated macrophage migration inhibitory factor (MIF). This resulted in ultrastructural changes that were observed using transmission electron microscopy (TEM). Four groups of female rats (6 in each group) were included in this study: sham-operated, ovariectomized (ovx), ovx that received estradiol benzoate (EB) injections intraperitoneally, and ovx that received YCJ orally. Two weeks after ovariectomy, two equidistant 1-cm full-thickness skin incisional wounds were made. At the end of the third week (7 day treatment) and the fourth week (14 day treatment) of study the rats were sacrificed, and their serum estradiol (E2) levels were measured by a chemiluminescent immunoassay. The skin from the wound was excised and examined by TEM and MIF immunohistochemical staining. The TEM study after 14 days of treatment showed that the size of the keratinocyte cells from the ovx+YCJ group was larger and these cells contained many more cytoplasmic processes than those of the ovx group. The MIF immunoreactivity was also lowest in the ovx+YCJ group. This study showed that there was an increased intercellular exchange via the cytoplasmic processes of the keratinocytes that could account for the promotion of cutaneous wound healing in the ovx rats receiving YCJ, and that the possible mechanism for this was via the down-regulation of MIF