Melody, an ENU mutation in Caspase 3, alters the catalytic cysteine residue and causes sensorineural hearing loss in mice

Progeny from the Harwell N-ethyl-N-nitrosourea (ENU) recessive mutagenesis screen were assessed for auditory defects. A pedigree was identified with multiple progeny lacking response to a clickbox test. Auditory brainstem response (ABR) analysis showed that homozygous mutant mice were profoundly deaf and the line was named melody. We subsequently mapped this mutation to a 6-Mb region on chromosome 8 and identified a point mutation in melody that results in a C163S substitution in the catalytic site of Caspase 3, a cysteine protease involved in apoptosis. Melody fails to complement a null Caspase-3 mutant. Scanning electron microscopy (SEM) has revealed disorganised sensory hair cells and hair cell loss. Histological analysis of melody has shown degeneration of spiral ganglion cells in homozygote mice, with a gradient of severity from apical to basal turns. Melody heterozygotes also show evidence of loss of spiral ganglion neurons, suggesting that the C163S mutation may show dominant negative effects by binding and sequestering proteins at the active site. The melody line provides a new model for studying the role of Caspase 3 in deafness and a number of other pathways and systems

Hand-in Hand, community music program : a case study

This thesis comprises a detailed study of the Hand-in-Hand (HiH) program, a community music program with a philosophy of making music for the benefit of others. In practical terms, the program involves school children visiting nursing homes to sing with, and activate the residents. This program is based in Canberra, in the Australian Capital Territory (ACT). It is organized and run by staff from the School of Music at the Australian National University, and from the ACT Education Department, and based primarily at Ainslie Primary School. The thesis explores and describes the HiH program including its philosophy, methodology, practical application and short-term outcomes. It discusses whether the program can be considered part of the intergenerational movement, music therapy or a behavior management program. Part 1 of the thesis focuses on a broad review of literature divided into three sections. Section 1 outlines the HiH program and its altruistic philosophy in the context of a broad range of literature pertaining to altruism. Section 2 explores HiH in relation to music therapy and intergenerational conventions. Section 3 discusses HiH as a music program with non-technical musical goals. Part 2 of the thesis is in three sections and describes the practical application of the program in three different situations. Section 1 examines the influence of the HiH program on the city-wide Music in Primary Schools program to which it belongs and specifically documents the Music Education Program at Ainslie Primary School. Section 2 outlines the methodology of an inclusive pilot project, involving students with special needs and mainstream children. Section 3 describes another pilot project involving high school children, with emotional and behavioral problems, visiting an aged-care facility and discusses the outcomes, and potential benefits of their involvement in the project. There is also a brief review of literature pertaining to the pilot projects at the beginning of both section two and three. In conclusion, the main findings are summarized and related to the literature. The many threads of the thesis are drawn together and suggestions are made regarding further in-depth study and research into the potential benefits of the program

Whalesong

Ballot decision upsets Regents -- UAS Library bids opened -- UAS logo selection questioned -- Knight greets year -- A Whaler welcome -- Enrollment up by 53 -- New page opens at bookstore -- Spring honor rolls announced -- NEWS BRIEF

The Clemlang, Spring 1978

https://tigerprints.clemson.edu/clemlang/1011/thumbnail.jp

Belle Voix Madrigal Singers Early Music Ensemble

Center for the Performing Arts Sunday Afternoon March 30, 2008 3:00p.m

The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation

Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis. Photorhabdus has an extensive secondary metabolism that is required for the interaction between the bacteria and the nematode. A major component of this secondary metabolism is a stilbene molecule, called ST. The first step in ST biosynthesis is the non-oxidative deamination of phenylalanine resulting in the production of cinnamic acid. This reaction is catalyzed by phenylalanine-ammonium lyase, an enzyme encoded by the stlA gene. In this study we show, using a stlA-gfp transcriptional fusion, that the expression of stlA is regulated by nutrient limitation through a regulatory network that involves at least 3 regulators. We show that TyrR, a LysR-type transcriptional regulator that regulates gene expression in response to aromatic amino acids in E. coli, is absolutely required for stlA expression. We also show that stlA expression is modulated by σS and Lrp, regulators that are implicated in the regulation of the response to nutrient limitation in other bacteria. This work is the first that describes pathway-specific regulation of secondary metabolism in Photorhabdus and, therefore, our study provides an initial insight into the complex regulatory network that controls secondary metabolism, and therefore mutualism, in this model organism