Acceptability and Feasibility of Reiki for Symptom Management in Children Receiving Palliative Care

Complementary therapies are chosen by parents of children receiving palliative care to augment the use of traditional medications for symptom management without the increased side effects additional medications may bring. Pain and anxiety are common symptoms for children receiving palliative care. Reiki therapy is a light touch therapy that has been examined in adults but not with children until recently. This dissertation addresses the evidence for complementary therapies for children experiencing pain and anxiety, Reiki therapy for pain and anxiety in adults, and evidence based complementary therapies for young children considering developmental stage. The main study is a quasi-experimental mixed methods pilot study design examining the acceptability and the feasibility of a Reiki therapy intervention for children ages 7 to 16 years receiving palliative care. We measured pain, anxiety, and relaxation operationalized as heart and respiratory rates pre and post Reiki therapy interventions at each of two home visits. We completed a structured interview separately with parents and children to elicit their views on the Reiki therapy experience. Paired student t-tests or Wilcoxon signed rank tests were calculated comparing the pre and post Reiki scores separately for verbal and non-verbal children for each treatment, over the entire intervention, and independent sample t-tests or Mann-Whitney tests comparing children based on demographic variables. We approached 24 child-parent dyads, 21 (87.5%) agreed to participate and signed consents while 3 (12.5%) declined to participate. Of the 21 dyads, 16 completed the study (eight verbal and eight non-verbal children). Statistical significance was obtained for verbal children for heart rate for treatment two (t=3.550, p = 0.009) and for nonverbal children for pain for treatment two (Z = -2.023, p = 0.063); however effect sizes using Cohen’s d levels were medium to large for both verbal and non-verbal children for pain and anxiety. Children and their parents told us their experiences with Reiki therapy. Themes found in interviews augment the quantitative results. Themes included Feeling Better, Hard to Judge, and Still Going On, which helped clarify the quantitative results. Results support further study of Reiki therapy for symptom management in children

A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice

Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose

One fungus, one name: defining the <i>genus Fusarium</i> in a scientifically robust way that preserves longstanding use

In this letter, we advocate recognizing the genus Fusarium as the sole name for a group that includes virtually all Fusarium species of importance in plant pathology, mycotoxicology, medicine, and basic research. This phylogenetically guided circumscription will free scientists from any obligation to use other genus names, including teleomorphs, for species nested within this clade, and preserve the application of the name Fusarium in the way it has been used for almost a century. Due to recent changes in the International Code of Nomenclature for algae, fungi, and plants, this is an urgent matter that requires community attention. The alternative is to break the longstanding concept of Fusarium into nine or more genera, and remove important taxa such as those in the F. solani species complex from the genus, a move we believe is unnecessary. Here we present taxonomic and nomenclatural proposals that will preserve established research connections and facilitate communication within and between research communities, and at the same time support strong scientific principles and good taxonomic practice

Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity

The rapid development of a SARS-CoV-2 vaccine is a global priority. Here, we develop two capsid-like particle (CLP)-based vaccines displaying the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. RBD antigens are displayed on AP205 CLPs through a split-protein Tag/Catcher, ensuring unidirectional and high-density display of RBD. Both soluble recombinant RBD and RBD displayed on CLPs bind the ACE2 receptor with nanomolar affinity. Mice are vaccinated with soluble RBD or CLP-displayed RBD, formulated in Squalene-Water-Emulsion. The RBD-CLP vaccines induce higher levels of serum anti-spike antibodies than the soluble RBD vaccines. Remarkably, one injection with our lead RBD-CLP vaccine in mice elicits virus neutralization antibody titers comparable to those found in patients that had recovered from COVID-19. Following booster vaccinations, the virus neutralization titers exceed those measured after natural infection, at serum dilutions above 1:10,000. Thus, the RBD-CLP vaccine is a highly promising candidate for preventing COVID-19

One Fungus, One Name: Defining the Genus Fusarium in a Scientifically Robust Way That Preserves Longstanding Use.

In this letter, we advocate recognizing the genus Fusarium as the sole name for a group that includes virtually all Fusarium species of importance in plant pathology, mycotoxicology, medicine, and basic research. This phylogenetically guided circumscription will free scientists from any obligation to use other genus names, including teleomorphs, for species nested within this clade, and preserve the application of the name Fusarium in the way it has been used for almost a century. Due to recent changes in the International Code of Nomenclature for algae, fungi, and plants, this is an urgent matter that requires community attention. The alternative is to break the longstanding concept of Fusarium into nine or more genera, and remove important taxa such as those in the F. solani species complex from the genus, a move we believe is unnecessary. Here we present taxonomic and nomenclatural proposals that will preserve established research connections and facilitate communication within and between research communities, and at the same time support strong scientific principles and good taxonomic practice