Peroxide impact on the fate of veterinary drugs in fertilizers

The presence of veterinary medicines in organic manure causes soil contamination which contributes to increasing resistance of indigenous microflora to drugs and results in greater susceptibility of people to allergies. The main aim of the study was to assess the efficiency of inorganic peroxide mixtures (PM) with calcium peroxide content (CaO2) in the stabilization process of manure contaminated with antiparasitic agents: albendazole (ALB) and levamisole (LEV). As a solid, CaO2 is relatively stable against decomposition. In contact with water, however, it hydrolyzes with release of oxygen. The hydrolyzation of CaO2 proceeds very slowly in soil, which guarantees the constant release of hydrogen peroxide that subsequently becomes the source of free radicals (chemical oxidation) and oxygen (aerobic conditions for the microbes). It may contribute to continuous elimination of drugs from manure. The study has demonstrated that there were significant differences in ALB and LEV conversion stimulated by the PM addition. PM supplementation increased the drug availability (on average 15% and 25% increase in the initial concentration for ALB and for LEV, respectively), thereby increasing the initial rate of reaction. Elimination of ALB and LEV from the manure sorption complex is followed by Ca2+ saturation. The initial degradation rate was affected by PM for both drugs, but the mechanisms of decomposition have been modified only for ALB. The loss of ALB in the peroxide supplemented samples was 92%, and in the samples, without the PM, it did not exceed 61%. Loss of LEV was over 90% irrespective of PM supplementation.Web of Science74132231

A pretreatment method for analysing albendazole by HPLC in plant material

Albendazole (ALB) belongs to a group of benzimidazoles—classified as antiparasitic pharmaceuticals. Its widespread application results in the presence of this pharmaceutical in natural environment (water and soil). In this paper a suitable pretreatment method was established including sampling, freezedrying and extraction. Vicia faba was used as model organism. ALB accumulation by plant tissues was observed in hydroponic culture as well as in soil. The range of pharmaceutical concentrations was 1.7×10−5 mol/L (in hydroponic culture) and 1.7×10−5 to 1.7×10−4 mol/kg air dry soil (in soil). Observations were conducted for 14 days. After this time biological material was freezedried and after homogenization, dimethyl sulfoxide (DMSO) extraction was performed. The recovery of ALB for the roots was 93 % while for the shoots 86 %. After cleaning, the samples were subjected to further analysis by HPLC system. Phosphate buffer and acetonitrile (50:50) were used as a mobile phase. Drug retention time was 6.3 min. Results obtained in this experiment indicate higher drug accumulation in roots rather than in the hypocotyl part of the plant, cultivated both in soil and in hydroponic culture.Web of Science2248art. no. 164

Short and long term orange dye effects over AOB and anammox activities

"This is the pre-peer reviewed version of the following article: Val del Río, A., Stachurski, A., Méndez, R., Campos, J. L., Surmacz-Górska, J., & Mosquera-Corral, A. (2017). Short- and long-term orange dye effects on ammonium oxidizing and anammox bacteria activities. Water Science and Technology, 76(1), 79-86, which has been published in final form at [http://dx.doi.org/10.2166/wst.2017.186. This article may be used for non-commercial purposes in accordance with IWA Publishing."In this research work the effects of orange azo dye over ammonia oxidizing bacteria (AOB) and anammox bacteria activities were tested. Adsorption onto the biomass was detected with both types of biomass however no biological decolourization occurred. Performed batch tests indicated that concentrations lower than 650 mgorange/L stimulated AOB activity while anammox bacteria activity was inhibited at concentrations higher than 25 mgorange/L. Long-term performance of both processes was tested in the presence of 50 mgorange/L. In the case of the partial nitritation process both the biomass concentration and the specific AOB activity increased after 50 days of orange azo dye addition. Regarding the anammox process, specific activity decreased down to 58% after 12 days of operation; however, initial values were restored 54 days after stopping the dye additionThis work was funded by the European Union through the Cost Action (ES-10755) and by the Spanish Government through FISHPOL (CTQ2014-55021-R) and GRANDSEA (CTM2014-55397-JIN) projects co-funded by FEDER. The authors from the USC belong to CRETUS (AGRUP2015/02) and the Galician Competitive Research Group (GRC 2013-032), programs co-funded by FEDERNO

The effect of temperature on the efficiency of industrial wastewater nitrification and its (geno)toxicity

The paper deals with the problem of the determination of the effects of temperature on the efficiency of the nitrification process of industrial wastewater, as well as its toxicity to the test organisms. The study on nitrification efficiency was performed using wastewater from one of Polish chemical factories. The chemical factory produces nitrogen fertilizers and various chemicals. The investigated wastewater was taken from the influent to the industrial mechanical-biological wastewater treatment plant (WWTP). The WWTP guaranteed high removal efficiency of organic compounds defined as chemical oxygen demand (COD) but periodical failure of nitrification performance was noted in last years of the WWTP operation. The research aim was to establish the cause of recurring failures of nitrification process in the above mentioned WWTP. The tested wastewater was not acutely toxic to activated sludge microorganisms. However, the wastewater was genotoxic to activated sludge microorganisms and the genotoxicity was greater in winter than in spring time. Analysis of almost 3 years’ period of the WWTP operation data and laboratory batch tests showed that activated sludge from the WWTP under study is very sensitive to temperature changes and the nitrification efficiency collapses rapidly under 16°C. Additionally, it was calculated that in order to provide the stable nitrification, in winter period the sludge age (SRT) in the WWTP should be higher than 35 days

Detection of antibiotic resistance genes in wastewater treatment plant – molecular and classical approach

Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confirmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L−1. N-acetylo-sulfamethoxazole concentration was 3349 ± 719 in winter and 2933 ± 429 ng L−1 in summer. Trimethoprim was present in wastewater at concentrations 400 ± 22 and 364 ± 60 ng L−1, respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confirmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary

Treatment and valorization of bio-waste in the EU

Moving away from fossil feedstock requires industrial sectors to switch to renewable raw materials. One option is the processing of biomass. However, agricultural, forestry and marine production of biomass cannot be expanded indefinitely. The linear value chains established in the fossil-based economy, leading from primary raw material to products and then to waste, are therefore no sustainable option. Instead circular value chains that recycle waste and make it available again as a raw material are needed. This also applies to bio-waste, as it arises in the primary production of biomass, as well as residual and waste materials from their processing, use and disposal. This paper reports on the volumes and current processing of bio- and biogenic wastes in the EU, presents recycling options that can lead to higher value added and employment as well as a lower environmental footprint, and also discusses the research, infrastructure and framework conditions needed

Current challenges and future perspectives for the full circular economy of water in European countries

This paper reviews the current problems and prospects to overcome circular water economy management challenges in European countries. The geopolitical paradigm of water, the water economy, water innovation, water management and regulation in Europe, environmental and safety concerns at water reuse, and technological solutions for water recovery are all covered in this review, which has been prepared in the frame of the COST ACTION (CA, 20133) FULLRECO4US, Working Group (WG) 4. With a Circular Economy approach to water recycling and recovery based on this COST Action, this review paper aims to develop novel, futuristic solutions to overcome the difficulties that the European Union (EU) is currently facing. The detailed review of the current environmental barriers and upcoming difficulties for water reuse in Europe with a Circular Economy vision is another distinctive aspect of this study. It is observed that the biggest challenge in using and recycling water from wastewater treatment plants is dealing with technical, social, political, and economic issues. For instance, geographical differences significantly affect technological problems, and it is effective in terms of social acceptance of the reuse of treated water. Local governmental organizations should support and encourage initiatives to expand water reuse, particularly for agricultural and industrial uses across all of Europe. It should not also be disregarded that the latest hydro politics approach to water management will actively contribute to addressing the issues associated with water scarcity