Global Climate Change And Its Impact On Indian Products: A Study On Patanjali Ayurved Limited

A decade ago Patanjali Ayurveda came with an influential dialogue “no chemicals & swadeshi alternative”. In a very few months, Patanjali has become a prime household name. Today, Patanjali Ayurveda Limited is as one of the main players in the Indian FMCG (Fast Moving Consumer Goods) company, and blindly trusted by the people across the country. Patanjali has now become the most prestigious brand in India and has a turnover of more than 2,000 crore in a very short span of time. Patanjali Ayurveda is offering the entire range of FMCG products such as food, grocery, nutrition, hair care, skin care and dental care at much competitive price than the other brands available in the market. The research “A Study of Brand Strategy and the Demand Inclination of the Patanjali Products in India” will provide the information of sales, marketing and the branding strategy of the Patanjali products. We will discuss the factor responsible for the growth of Patanjali Ayurvedic Limited and the future projects by Patanjali Ayurvedic Limited

PHYTOSYNTHESIS OF SILVER NANOPARTICLES USING HYGROPHILA AURICULATA LEAF EXTRACT AND ASSESSMENT OF THEIR ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES

Objective: In this study, the phytosynthesis of silver nanoparticles (AgNPs) using leaf extracts of Hygrophila auriculata (HA) and their biological activities was investigated.Methods: The synthesis of AgNPs was done by using the green synthetic method and analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and further characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The antibacterial activity of HA AgNPs was studied by agar well diffusion method and micro broth dilution method for determination of minimum inhibitory concentration (MIC). The antioxidant potentials of HA AgNPs were detected by Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay.Results: The prepared HA AgNPs showed characteristic absorption peak at 420 nm in the UV-Vis spectrum. FTIR spectra had shown that the biomolecules present in leaf extract were responsible for the reduction and capping material of silver nanoparticles. XRD study showed the particles found to be crystalline in nature, with a face-centered cubic (fcc) structure. TEM and DLS results revealed that the AgNPs were mostly spherical with an average size ranging from ~15-115 nm with a mean diameter of 40.96 nm. The HA AgNPs showed good antibacterial activity and MIC against Staphylococcus aureus (ATCC 6538), Bacillus cereus (NCIM 2106), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739). In agar well diffusion method, the maximum zone of inhibition was found against Pseudomonas aeruginosa (ATCC 9027) with 18 mm and minimum zone of inhibition was found to be against Escherichia coli (ATCC 8739) with 13 mm. The MIC of the HA AgNp was found to be 5µg against all the test organisms. In addition, the Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay revealed they can be used as the potential scavenger against deleterious damages caused by the free radicals.Conclusion: The present study explored that Hygrophila auriculata which are efficient producers of AgNPs and could act as safe and cost-effective with potential antibacterial and antioxidant activities. These findings encourage studying HA AgNP further for their potential biological applications

Phenomenon of Summer Apartments in the First Half of 20th Century

Summary: This work is dedicated to phenomenon of summer apartments in the 1st half of 20th century,especialy to village Senohraby, which was used mainly as an example. This work tries to show means of spending free time in summer resorts and means of entertainment specifically in the surroundings of Prague, because of it's nearness to capital city.Important part of this work is dedicated to question, what sorts of guests were spending times in this summer resorts. And this work also wants to show some aspects of life before First World War and during 1.st republic in Czechoslovakia

Stability and rheological study on carbon-based nanofluids

In this study, an organic derived nanofluid has been developed from bio-origin resources. Carbon nanopowder (CNP) is obtained from derived rice husk and was prepared via a simple two-steps thermal process with minimum energy (low temperature and reaction time) using solar assisted plasma furnace. Nanofluids comprised of CNP and EG/water binary mixture has been prepared at various concentration such as 0.02 – 0.10 vol% of CNP. Flow curve of nanofluids showed that at minimum inclusion of CNP improved the stress of the fluid significantly. More to the addition, dynamic viscosity measure possesses that addition of CNP stabilized the properties of the fluid compared to virgin base fluid. Moreover, the stability results showed that the nanofluids stabilized starting from 1 week onwards as evidenced by UV-Visible spectrophotometer analysis. Furthermore, little to no precipitate noticed even after 8 weeks. This work offers greener approach for nanofluids which organic derived and environmentally friendly (very low percentage of nanoparticle, 0.02 vol%

A method for high‐throughput production of sequence‐verified DNA libraries and strain collections

Abstract The low costs of array‐synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We have developed a cost‐effective method called Recombinase Directed Indexing (REDI), which involves integration of a complex library into yeast, site‐specific recombination to index library DNA, and next‐generation sequencing to identify desired clones. We used REDI to generate a library of ~3,300 DNA probes that exhibited > 96% purity and remarkable uniformity (> 95% of probes within twofold of the median abundance). Additionally, we created a collection of ~9,000 individually accessible CRISPR interference yeast strains for > 99% of genes required for either fermentative or respiratory growth, demonstrating the utility of REDI for rapid and cost‐effective creation of strain collections from oligonucleotide pools. Our approach is adaptable to any complex DNA library, and fundamentally changes how these libraries can be parsed, maintained, propagated, and characterized