Visualization of facet-dependent pseudo-photocatalytic behavior of TiO2 nanorods for water splitting using In situ liquid cell TEM

We report an investigation of the pseudo-photocatalytic behavior of rutile TiO2 nanorods for water splitting observed with liquid cell transmission electron microscopy (TEM). The electron beam serves as a “light” source to initiate the catalytic reaction and a “water-in-salt” aqueous solution is employed as the electrolyte. The observation reveals that bubbles are generated preferentially residing near the {110} facet of a rutile TiO2 nanorod under a low electron dose rate (9.3–18.6 e-/Å2·s). These bubbles are ascribed to hydrogen gas generated from the pseudo-photocatalytic water splitting. As the electron beam current density increases to 93 e-/Å2 ·s, bubbles are also found at the {001} and {111} facets as well as in the bulk liquid solution, demonstrating the dominant effects of water electrolysis by electron beam under higher dose rates. The facet-dependent pseudo-photocatalytic behavior of rutile TiO2 nanorods is further validated using density functional theory (DFT)calculation. Our work establishes a facile liquid cell TEM setup for the study of pseudo-photocatalytic water splitting and it may also be applied to investigation of other photo-activated phenomena occurring at the solid-liquid interfaces

Effects of different solutions consumed during exercise on cognitive function of male college soccer players

Background/Objectives: The present study aimed to investigate the effects of three solutions, i.e. carbohydrate-electrolyte-solution (CES), carbohydrate-electrolyte-protein-solution (CEPS), and placebo (PLA), on cognitive function of college soccer players. Methods: Sixteen male college soccer players completed three main trials in a randomized cross-over study design. In each main trial, participants completed 90 min Loughborough Intermittent Shuttle Test (LIST) protocol and consumed one of three solutions. The cognitive function tests were performed; blood glucose and lactate concentrations, and several subjective measurements were also recorded in each trial. Results: Compared with pre-exercise level, the accuracy of Rapid Visual Information Processing test (RVIPT) and the response time in Visual Search Test (VST, complex level) after LIST improved in CES and CEPS trials, but not in PLA trial. However, the accuracy of VST (complex level) decreased in both CES and CEPS trials, compared with PLA trial. CEPS consumption improved accuracy in VST (simple level), compared with CES consumption. Blood glucose concentrations were well maintained in CEPS trial, but not in CES and PLA trials. Conclusion: It seems that both CES and CEPS consumption show certain benefits on some aspects of cognitive function in male college soccer players in Hong Kong. However, these effects may be specific to the cognitive domain tested

Effects of carbohydrate and protein co-ingestion during short-term moderate-intensity exercise on cognitive function

BACKGROUND: This study aimed to investigate the effects of isocaloric carbohydrate-electrolyte solution (CES) and carbohydrates-electrolyte-protein solution (CEPS) consumed during moderate-intensity exercise on cognitive function. METHODS: Twenty-three healthy participants (age: 21.7±1.5 years, Body Mass Index: 21.8±2.0 kg/m2, and peak oxygen consumption (VO2peak): 42.4±6.9 mL/kg/min) were recruited in the study. Participants completed two main experiment trials using a cross-over study design. In each trial, they completed 30 minutes of 70% VO2peak cycling, and one of two solutions (CES or CEPS) was consumed. A battery of cognitive function tests (imPACT Package) was administrated at rest and immediately post-exercise. RESULTS: Blood glucose concentrations decreased in both trials. Heart rate and blood lactate concentrations increased in both trials. However, no significant main effects of the exercise on all cognitive function variables (verbal and visual memory, visual-motor speed, reaction time, impulse control, and cognitive efficiency index) were observed (all P>0.05). Also, there was no difference in any of the cognitive function variables between the CES and CEPS trials (all P>0.05). CONCLUSIONS: There was no difference in the cognitive performance when CES or CES-PRO solutions was consumed during 30 minutes moderate-intensity cycling

Clinical identification of bacteria in human chronic wound infections: Culturing vs. 16S ribosomal DNA sequencing

Background: Chronic wounds affect millions of people and cost billions of dollars in the United States each year. These wounds harbor polymicrobial biofilm communities, which can be difficult to elucidate using culturing methods. Clinical molecular microbiological methods are increasingly being employed to investigate the microbiota of chronic infections, including wounds, as part of standard patient care. However, molecular testing is more sensitive than culturing, which results in markedly different results being reported to clinicians. This study compares the results of aerobic culturing and molecular testing (culture-free 16S ribosomal DNA sequencing), and it examines the relative abundance score that is generated by the molecular test and the usefulness of the relative abundance score in predicting the likelihood that the same organism would be detected by culture.Methods: Parallel samples from 51 chronic wounds were studied using aerobic culturing and 16S DNA sequencing for the identification of bacteria.Results: One hundred forty-five (145) unique genera were identified using molecular methods, and 68 of these genera were aerotolerant. Fourteen (14) unique genera were identified using aerobic culture methods. One-third (31/92) of the cultures were determined to be < 1% of the relative abundance of the wound microbiota using molecular testing. At the genus level, molecular testing identified 85% (78/92) of the bacteria that were identified by culture. Conversely, culturing detected 15.7% (78/497) of the aerotolerant bacteria and detected 54.9% of the collective aerotolerant relative abundance of the samples. Aerotolerant bacterial genera (and individual species including Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis) with higher relative abundance scores were more likely to be detected by culture as demonstrated with regression modeling.Conclusion: Discordance between molecular and culture testing is often observed. However, culture-free 16S ribosomal DNA sequencing and its relative abundance score can provide clinicians with insight into which bacteria are most abundant in a sample and which are most likely to be detected by culture. © 2012 Rhoads et al.; licensee BioMed Central Ltd

Multi-seeded melt growth (MSMG) of bulk Y-Ba-Cu-O using thin-film seeds

Y-Ba-Cu-O (YBCO) and Sm-Ba-Cu-O (SmBCO) thin films have been used for the first time as heterogeneous seeds to multi-seed successfully the melt growth of bulk YBCO in a multi-seeded melt growth (MSMG) process. The use of thin film seeds, which may be prepared with highly controlled orientation (i.e. with a well-defined a-b plane and precisely known a-direction), is based on their superheating properties and reduces significantly contamination of the bulk sample by the seed material. A variety of grain boundaries were obtained by varying the angle between the seeds. Microstructural studies indicate that the extent of residual melt deposited at the grain boundary decreases with increasing grain boundary contact angle. It is established that the growth front proceeds continuously at the (110)/(110) grain boundary without trapping liquid, which leads to the formation of a clean grain boundary

Association between nutritional status, physical fitness and executive functions in preadolescents: a person-centered approach

Objective: In the current study, a person-centered approach was adopted to investigate the relationship between nutritional status and physical fitness profiles and executive functions (EF) in preadolescents. Methods: Participants (Mage = 10.8 years; Male = 50.8%) were recruited from two primary schools in Hong Kong. Nutritional status [body mass index (BMI)], physical fitness including cardiorespiratory fitness (CRF, predicted VO2max, multi-stage fitness test) and speed-agility (20-m sprint) were measured on school days. EF performance was measured using the Flanker task (inhibition) and the Sternberg task (working memory). Results: Data from 120 preadolescents were considered valid. Three distinct profiles were identified by a person-centered approach. Profile 1 was featured by high BMI (21.61 ± 3.38 kg/m2), poor VO2max (33.29 ± 23.96 ml/kg/min), and slow 20-m sprint (4.51 ± 0.13 s). Profile 2 was featured by low BMI (15.99 ± 3.38), fair VO2max (44.98 ± 23.96) and fast 20-m sprint (3.97 ± 0.13). Profile 3 was featured by low BMI (15.63 ± 3.38), poor VO2max (32.37 ± 23.96), and slow 20-m sprint (4.48 ± 0.13). Wald chi-square test revealed preadolescents in profile 1 and profile 2 performed better than profile 3 in accuracy of Flanker task (1 vs. 3: χ2 = 12.23, P < 0.001; 2 vs. 3: χ2 = 10.86, P = 0.001). That is, for normal weight preadolescents with poor CRF and speed-agility, those with superior nutritional status performed better in inhibition. For normal weight preadolescents with poor nutritional status, those with superior CRF and speed-agility had better inhibitory capacity. Conclusion: Compared to the commonly used variable-centered approach, this person-centered approach is a valuable addition that expands the understanding of the association between nutritional status, physical fitness and EF in preadolescents. Results are discussed with regards to maximizing health behaviors and implications for educational policy

Robust Detection of Hierarchical Communities from Escherichia coli Gene Expression Data

Determining the functional structure of biological networks is a central goal of systems biology. One approach is to analyze gene expression data to infer a network of gene interactions on the basis of their correlated responses to environmental and genetic perturbations. The inferred network can then be analyzed to identify functional communities. However, commonly used algorithms can yield unreliable results due to experimental noise, algorithmic stochasticity, and the influence of arbitrarily chosen parameter values. Furthermore, the results obtained typically provide only a simplistic view of the network partitioned into disjoint communities and provide no information of the relationship between communities. Here, we present methods to robustly detect coregulated and functionally enriched gene communities and demonstrate their application and validity for Escherichia coli gene expression data. Applying a recently developed community detection algorithm to the network of interactions identified with the context likelihood of relatedness (CLR) method, we show that a hierarchy of network communities can be identified. These communities significantly enrich for gene ontology (GO) terms, consistent with them representing biologically meaningful groups. Further, analysis of the most significantly enriched communities identified several candidate new regulatory interactions. The robustness of our methods is demonstrated by showing that a core set of functional communities is reliably found when artificial noise, modeling experimental noise, is added to the data. We find that noise mainly acts conservatively, increasing the relatedness required for a network link to be reliably assigned and decreasing the size of the core communities, rather than causing association of genes into new communities.Comment: Due to appear in PLoS Computational Biology. Supplementary Figure S1 was not uploaded but is available by contacting the author. 27 pages, 5 figures, 15 supplementary file

Rational Redesign of Glucose Oxidase for Improved Catalytic Function and Stability

Glucose oxidase (GOx) is an enzymatic workhorse used in the food and wine industries to combat microbial contamination, to produce wines with lowered alcohol content, as the recognition element in amperometric glucose sensors, and as an anodic catalyst in biofuel cells. It is naturally produced by several species of fungi, and genetic variants are known to differ considerably in both stability and activity. Two of the more widely studied glucose oxidases come from the species Aspergillus niger (A. niger) and Penicillium amagasakiense (P. amag.), which have both had their respective genes isolated and sequenced. GOx from A. niger is known to be more stable than GOx from P. amag., while GOx from P. amag. has a six-fold superior substrate affinity (KM) and nearly four-fold greater catalytic rate (kcat). Here we sought to combine genetic elements from these two varieties to produce an enzyme displaying both superior catalytic capacity and stability. A comparison of the genes from the two organisms revealed 17 residues that differ between their active sites and cofactor binding regions. Fifteen of these residues in a parental A. niger GOx were altered to either mirror the corresponding residues in P. amag. GOx, or mutated into all possible amino acids via saturation mutagenesis. Ultimately, four mutants were identified with significantly improved catalytic activity. A single point mutation from threonine to serine at amino acid 132 (mutant T132S, numbering includes leader peptide) led to a three-fold improvement in kcat at the expense of a 3% loss of substrate affinity (increase in apparent KM for glucose) resulting in a specify constant (kcat/KM) of 23.8 (mM−1 · s−1) compared to 8.39 for the parental (A. niger) GOx and 170 for the P. amag. GOx. Three other mutant enzymes were also identified that had improvements in overall catalysis: V42Y, and the double mutants T132S/T56V and T132S/V42Y, with specificity constants of 31.5, 32.2, and 31.8 mM−1 · s−1, respectively. The thermal stability of these mutants was also measured and showed moderate improvement over the parental strain

Trilobite extinctions, facies changes and the ROECE carbon isotope excursion at the Cambrian Series 2 - 3 boundary, Great Basin, western USA

The mass extinction of the olenellid trilobites occurred around the Cambrian Series 2 - Series 3 boundary. Like many other crises, it coincided with a negative carbon isotope excursion but the associated palaeoenvironmental changes remain unclear. To investigate the causal mechanism for this event, we report facies changes, pyrite framboid petrography and carbon isotope values from Cambrian Series 2 - Series 3 (traditionally Early - Middle Cambrian) boundary strata of the Carrara 20 Formation (Death Valley region, California) and Pioche Formation (Nevada). These data reveal regionally changing water depths from high-energy, nearshore facies (oolitic grainstone) to more offshore silty marl and finer-grained carbonate mudstone. In the Carrara Formation, the series boundary occurs within a deepening succession, transitioning from high-energy, nearshore facies (oolitic grainstone and oncolitic packstone) to offshore marl, the latter of which contains pyrite framboid populations indicative of low-oxygen (dysoxic) depositional conditions. Intermittent dysoxia persisted below sub-wave base settings throughout the early and middle Cambrian, but did not intensify at the time of extinction, arguing against anoxia as a primary cause in the olenellid trilobite extinction. Within both field areas, the extinction interval coincided with a minimum in d13Ccarb values, which we interpret as the regional manifestation of the Redlichiid-Olenellid Extinction Carbon isotope Excursion (ROECE). The Series 2 - Series 3 boundary is reported to closely coincide with a large-amplitude sea-level fall that produced the Sauk I/II sequence boundary, but the placement of the Series 2 - Series 3 boundary within a transgressive interval of the Carrara Formation shows that this is not the case. The main sequence boundary in the succession occurs much lower in the succession (at the top of the Zabriskie Quartzite) and therefore precedes the extinction of the olenellids and ROECE