Study on atomization mechanisms and spray fragmentation characteristics of water and emulsion butachlor

Agricultural chemicals are commonly used to control pests and weeds, but cause pesticide waste problems. Oil-based emulsions are often used as pesticide formulations to improve pesticide utilization. In this study, the spray visualization experiment of the water and oil-based emulsion butachlor is carried out using an ST flat fan nozzle at 0.1–0.5 MPa pressure. The dimensionless method is used to analyze the difference in liquid sheet fragmentation morphology and disintegration process and the influence of different fragmentation methods on droplet size. It is found that the hydrophobic components in pesticide have a significant effect on the morphology and process of atomization fragmentation. When spray liquid is water, the liquid sheet breaks up into liquid ligaments due to the Rayleigh instability, then the ligaments break up into droplets. The side view of a liquid sheet is a large-amplitude wave disturbance. When the spray liquid is the emulsion butachlor, holes are generated on the liquid sheet, then the holes break up into droplets. The fragmentation method of emulsion spray is the perforation mechanism. Compared with water spray, the presence of the pesticide butachlor increases the droplet size and spray angle and improves the uniformity of droplet size distribution but reduces the breakup length. The spray angle shows a power law dependence of the Weber number with a power of 0.17 for all conditions tested here. At 0.3 MPa, DV50 increases 25%, and span decreases from 1.187 to 1.172. This study could provide reference for the addition of agricultural additives, the improvement of spray operation efficiency, and the establishment of spray fragmentation mechanism

Ripk3 signaling regulates HSCs during stress and represses radiation-induced leukemia in mice

Receptor-interacting protein kinase 3 (Ripk3) is one of the critical mediators of inflammatory cytokine-stimulated signaling. Here we show that Ripk3 signaling selectively regulates both the number and the function of hematopoietic stem cells (HSCs) during stress conditions. Ripk3 signaling is not required for normal homeostatic hematopoiesis. However, in response to serial transplantation, inactivation of Ripk3 signaling prevents stress-induced HSC exhaustion and functional HSC attenuation, while in response to fractionated low doses of ionizing radiation (IR), inactivation of Ripk3 signaling accelerates leukemia/lymphoma development. In both situations, Ripk3 signaling is primarily stimulated by tumor necrosis factor-α. Activated Ripk3 signaling promotes the elimination of HSCs during serial transplantation and pre-leukemia stem cells (pre-LSCs) during fractionated IR by inducing Mlkl-dependent necroptosis. Activated Ripk3 signaling also attenuates HSC functioning and represses a pre-LSC-to-LSC transformation by promoting Mlkl-independent senescence. Furthermore, we demonstrate that Ripk3 signaling induces senescence in HSCs and pre-LSCs by attenuating ISR-mediated mitochondrial quality control

Experimental Study of the Droplet Deposition Characteristics on an Unmanned Aerial Vehicle Platform under Wind Tunnel Conditions

Unmanned aerial vehicles (UAVs) are widely used in field pesticide spray operations due to their wide applicability and high operational efficiency. However, their high spray height and fine pesticide droplets lead to a greater risk of drift and likely different droplet deposition outcomes compared to the expectation. So far, most of the previous studies have used direct field methods on UAVs’ droplet deposition characteristics and there have been few carried out in wind tunnels. Thus, in this paper, a simulated UAV platform equipped with TeeJet 80-015 VP fan nozzles was utilized to study the droplet deposition characteristics in a wind tunnel. The droplet deposition amount and drift potential reduction percentage (DPRP) under different spray parameters were obtained. The results showed that when the rotor was open, the deposition amount in the target area increased by 2.6 times and the drift deposition amount decreased by 7.3 times when spraying tap water at 3 m/s wind speed and 3 bar pressure. Faster wind speeds led to greater drift deposition amounts and a lower DPRP, but higher pressures resulted in greater drift deposition amounts and a larger DPRP. The 30 g/L PEG-20000 solution has a higher droplet size and smaller relative droplet spectrum width RS, resulting in the deposition amount in the target area increasing by 9.13% on average and the drift amount decreasing by 24.7% on average, and it can be used as an anti-drift additive when needed. The research results can provide reference and technical support for UAV wind tunnel tests and field operation specifications

Heat treatment, microstructure and mechanical properties of a Mg-Gd-Y alloy grain-refined by Al additions

The effect of additions of 0.5-1 wt% Al on grain refinement, heat treatment and mechanical properties of Mg-10Gd-3Y (wt%) alloy has been investigated. The additions of 0.6-1 wt% Al showed a dramatic grain refinement effect, which is associated with the formation of the Al(GdY) particles that act as nucleants for α-Mg grains. Compared to the Zr refined counterpart, the Al refined alloy needed to be solution treated at a higher temperature to dissolve the majority of the intermetallic particles. Subsequent ageing at low temperatures led to pronounced precipitation hardening and consequently a large improvement in strength. In the peak-aged condition, the strength of the Al refined alloy is comparable to that of the Zr refined counterpart

Effects of sprayer speed, spray distance, and nozzle arrangement angle on low-flow air-assisted spray deposition

Air-assisted spraying technology is widely used in orchard sprayers to disturb canopy leaves and force droplets into the plant canopy to reduce droplet drift and increase spray penetration. A low-flow air-assisted sprayer was developed based on a self-designed air-assisted nozzle. The effects of the sprayer speed, spray distance, and nozzle arrangement angle on the deposit coverage, spray penetration, and deposit distribution were investigated in a vineyard by means of orthogonal tests. The optimal working conditions for the low-flow air-assisted sprayer working in the vineyard were determined as a sprayer speed of 0.65m/s, a spray distance of 0.9m, and a nozzle arrangement angle of 20°. The deposit coverages of the proximal canopy and intermediate canopy were 23.67% and 14.52%, respectively. The spray penetration was 0.3574. The variation coefficients of the deposit coverage of the proximal canopy and intermediate canopy, which indicate the uniformity of the deposition distribution, were 8.56% and 12.33%, respectively

Mechanistic Basis for In Vivo Therapeutic Efficacy of CK2 Inhibitor CX-4945 in Acute Myeloid Leukemia

Protein Kinase CK2 (Casein Kinase 2 or CK2) is a constitutively active serine-threonine kinase overactive in human malignancies. Increased expression and activity of CK2 in Acute Myeloid Leukemia (AML) is associated with a poor outcome. CK2 promotes AML cell survival by impinging on multiple oncogenic signaling pathways. The selective small-molecule CK2 inhibitor CX-4945 has shown in vitro cytotoxicity in AML. Here, we report that CX-4945 has a strong in vivo therapeutic effect in preclinical models of AML. The analysis of genome-wide DNA-binding and gene expression in CX-4945 treated AML cells shows that one mechanism, by which CK2 inhibition exerts a therapeutic effect in AML, involves the revival of IKAROS tumor suppressor function. CK2 phosphorylates IKAROS and disrupts IKAROS’ transcriptional activity by impairing DNA-binding and association with chromatin modifiers. Here, we demonstrate that CK2 inhibition decreases IKAROS phosphorylation and restores IKAROS binding to DNA. Further functional experiments show that IKAROS negatively regulates the transcription of anti-apoptotic genes, including BCL-XL (B cell Lymphoma like–2 like 1, BCL2L1). CX-4945 restitutes the IKAROS-mediated repression of BCL-XL in vivo and sensitizes AML cells to apoptosis. Using CX-4945, alongside the cytotoxic chemotherapeutic drug daunorubicin, augments BCL-XL suppression and AML cell apoptosis. Overall, these results establish the in vivo therapeutic efficacy of CX-4945 in AML preclinical models and determine the role of CK2 and IKAROS in regulating apoptosis in AML. Furthermore, our study provides functional and mechanistic bases for the addition of CK2 inhibitors to AML therapy